Fungal biomass in pastures increases with age and reduced N input

Previous studies have shown that soil fungal biomass increases towards more natural, mature systems. Shifts to a fungal-based soil food web have previously been observed with abandonment of agricultural fields and extensification of agriculture. In a previous field experiment we found increased fungal biomass with reduced N fertilisation. Here, we explore relationships between fungi, bacteria, N input and grassland age on real dairy farms in the Netherlands. We hypothesised that also in pastures that are still in production there is a negative relationship between fungal biomass and fertilisation, and that fungal biomass increases with grassland age in pastures that are still in production. We expected the fungal/bacterial biomass ratio to show the same responses, as this ratio has often been used as an indicator for management changes. We sampled 48 pastures from eight organic dairy farms. Sites differed in age and fertilisation rate. We determined fungal and bacterial biomass, as well as ergosterol (a fungal biomarker). Fungal and bacterial biomass and ergosterol, showed a negative relationship with N application rate, and correlated positively with organic matter percentage. In old pastures, fungal biomass and ergosterol were higher than in younger pastures. Because bacterial biomass responded in the same way as fungal biomass, the F/B ratio remained constant, and can therefore—in our data set—not be used as an indicator for changing management. We conclude that the changes in fungal and bacterial biomass were driven by changes in organic matter quality and quantity. The negative relationship we found between N application rate and fungal biomass adds to earlier work and confirms the presence of this relationship in pastures with relatively small differences in management intensities. Earlier studies on shifts in fungal biomass focused on ex-agricultural fields or restoration projects. Here we show that fungal biomass is also higher in older agricultural pastures.     

Microbial use of organic amendments in saline soils monitored by changes in the C/C ratio

An incubation experiment was carried out to investigate whether salinity at high pH has negative effects on microbial substrate use, i.e. the mineralization of the amendment to CO₂ and inorganic N and the incorporation of amendment C into microbial biomass C. In order to exploit natural differences in the ¹³C/¹²C ratio, substrate from two C₄ plants, i.e. highly decomposed and N-rich sugarcane filter cake and less decomposed N-poor maize leaf straw, were added to two alkaline Pakistani soils differing in salinity, which had previously been cultivated with C₃ plants. In soil 1, the additional CO₂ evolution was equivalent to 65% of the added amount in the maize straw treatment and to 35% in the filter cake treatment. In the more saline soil 2, the respective figures were 56% and 32%. The maize straw amendment led to an identical immobilization of approximately 48 μg N g⁻¹ soil over the 56-day incubation in both soils compared with the control soils. In the filter cake treatment, the amount of inorganic N immobilized was 8.5 μg N g⁻¹ higher in soil 1 than in soil 2 compared with the control soils. In the control treatment, the content of microbial biomass C₃-C in soil 1 was twice that in soil 2 throughout the incubation. This fraction declined by about 30% during the incubation in both soils. The two amendments replaced initially similar absolute amounts of the autochthonous microbial biomass C, i.e. 50% of the original microbial biomass C in soil 1 and almost 90% in soil 2. The highest contents of microbial biomass C₄-C were equivalent to 7% (filter cake) and 11% (maize straw) of the added C. In soil 2, the corresponding values were 14% lower. Increasing salinity had no direct negative effects on microbial substrate use in the present two soils. Consequently, the differences in soil microbial biomass contents are most likely caused indirectly by salinity-induced reduction in plant growth rather than directly by negative effects of salinity on soil microorganisms.     

Comparison of antioxidant potency of commonly consumed polyphenol-rich beverages in the United States

A number of different beverage products claim to have antioxidant potency due to their perceived high content of polyphenols. Basic and applied research indicates that pomegranate juice (PJ), produced from the Wonderful variety of Punica granatum fruits, has strong antioxidant activity and related health benefits. Although consumers are familiar with the concept of free radicals and antioxidants, they are often misled by claims of superior antioxidant activity of different beverages, which are usually based only on testing of a limited spectrum of antioxidant activities. There is no available direct comparison of PJ's antioxidant activity to those of other widely available polyphenol-rich beverage products using a comprehensive variety of antioxidant tests. The present study applied (1) four tests of antioxidant potency [Trolox equivalent antioxidant capacity (TEAC), total oxygen radical absorbance capacity (ORAC), free radical scavenging capacity by 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP)]; (2) a test of antioxidant functionality, that is, inhibition of low-density lipoprotein (LDL) oxidation by peroxides and malondialdehyde methods; and (3) evaluation of the total polyphenol content [by gallic acid equivalents (GAEs)] of polyphenol-rich beverages in the marketplace. The beverages included several different brands as follows: apple juice (3), a?aí juice (3), black cherry juice (3), blueberry juice (3), cranberry juice (3), Concord grape juice (3), orange juice (3), red wines (3), iced tea beverages (10) [black tea (3), green tea (4), white tea (3)], and a major PJ available in the U.S. market. An overall antioxidant potency composite index was calculated by assigning each test equal weight. PJ had the greatest antioxidant potency composite index among the beverages tested and was at least 20% greater than any of the other beverages tested. Antioxidant potency, ability to inhibit LDL oxidation, and total polyphenol content were consistent in classifying the antioxidant capacity of the polyphenol-rich beverages in the following order: PJ>red wine>Concord grape juice>blueberry juice>black cherry juice, a?aí juice, cranberry juice>orange juice, iced tea beverages, apple juice. Although in vitro antioxidant potency does not prove in vivo biological activity, there is also consistent clinical evidence of antioxidant potency for the most potent beverages including both PJ and red wine.     

Considerations regarding the use of hyperspectral imaging data in classifications of food products, exemplified by analysis of maize kernels

Development of robust analytical procedures is critical when using hyperspectral imaging technology in food technology and agriculture. This study used near-isogenic inbred corn lines to address two basic questions: (1) To what extent is classification accuracy increased by grinding maize kernels? (2) Can the classification accuracy of two near-isogenic inbred lines be increased by using a spectral filter to classify only certain hyperspectral profiles from each image cube? Whole kernels and ground kernels in two particle intervals, 0.250-0.354 mm (size 1) and 0.354-0.841 mm (size 2), were examined. Spectral profiles acquired from ground kernels had higher spectral repeatability than data collected from whole kernels. The classification error of discriminant functions from whole kernels was >3 times lower than that of size 1 ground particles. Applying a spectral filter to input data had negligible effect on classifications of hyperspectral profiles from whole kernels and size 2 ground particles, but for size 1 ground particles a considerable increase in accuracy was observed. Independent validation confirmed that distinction between wild type and mutant inbred maize lines could be conducted with >80% accuracy after the proposed spectral filter had been applied to hyperspectral profiles of size 1 ground particles. A combination of discriminant analysis and regression analysis could be used to accurately predict mixture ratios of the two inbred lines. The use of spectral filtering to increase the level of spectral repeatability and the use of hyperspectral imaging technology in large-scale commercial operations are discussed.     

Potential chemical time bombs in the schelde estuary

The Schelde estuary is heavily polluted with many different (micro)pollutants. This results in high concentrations in the abiotic and biotic compartments of the Schelde estuary and in various effects. the present day suboxic and anoxic conditions in the upper estuary are probably responsible for the high distribution coefficients for some trace metals in the lower Schelde estuary (Western Schelde). a future reduction in discharges with a high biological oxygen demand will therefore not only result in higher oxygen concentrations, but probably also in lower distribution coefficients for trace metals in the Western Schelde. Simulations show that lower trace metal distribution coefficients will probably result in higher concentrations of dissolved (bioavailable) trace metals, even with substantially reduced discharge rates, due to the desorption of trace metals from resuspended bottom sediments.     

Soil erosion and redistribution on cultivated and uncultivated land near las bardenas in the central Ebro river Basin,Spain

The semiarid regions of Spain, including the central part of the Ebro River basin, are under threat due to desertification. Severe erosion, as a result of poor land management, has led to degradation of the soil resource, and there is a clear need for quantitative erosion rate data to evaluate the problem. This study aimed to examine the potential for using caesium-137 to identify the patterns and rates of soil erosion and redistribution within this semiarid environment. Samples for the determination of caesium-137 were collected from uncultivated slopes and cultivated valley floor sites near the head and outlet of a small representative basin in the Las Bardenas area. The measured patterns of caesium-137 mobilization, redistribution and export provide a semiquantitative indication of the variation in erosion within the study site. Calibration of the caesium-137 measurements, taking account of the differing behaviour of radiocaesium on cultivated and uncultivated land, allows estimation of the actual rates of erosion and deposition involved. The results show (1) the erosion rates on the cultivated land (1.6–2.5 kg m⁻² yr⁻¹) are typically more than five times those seen on the uncultivated land (0.2–0.4 kg m⁻² yr⁻¹), and (2) erosion on the uncultivated land is significantly less severe at the head of the basin than at the outlet. Study of the vegetation cover suggests that lower growing shrubs and grasses may be more effective in reducing erosion in this environment than trees.     

Agroforestry techniques for the rehabilitation of degraded salt-affected lands in India

The development of appropriate technologies for the judicious use of India's 8.11 million ha of salt-affected lands would give increased food, fodder and fuelwood production. A sizeable portion of the salt-affected lands of the Indo-Gangetic plain have been reclaimed through chemical amendments and are being commercially used for arable farming. However, large areas of salt-affected common lands, village and government lands and waste lands near cities, along railway tracks and roads do not have any productive use. Owing to their sparse vegetative cover such lands are vulnerable to further degradation and can be a source of runoff causing floods, especially where cows congregate. Research at the Central Soil Salinity Research Institute, Karnal has generated agroforestry techniques which could enable the economic exploitation of such marginal lands. This paper deals with various aspects of these techniques, which have been applied to over 50 000 ha by 1994. Based on eight to ten years of growth and biomass figures, the most salt-tolerant woody species identified were: Prosopis juliflora, Acacia nilotica, Casuarina equisetifolia, Tamarix articulata, Leptochloa fusca (a palatable forage grass which was found to be a promising primary colonizer of salt lands). Agronomic practices for the successful establishment of trees such as planting methods, amendment use, irrigation, spacing and lopping schedules are discussed. A Prosopis juliflora-leptochloa fusca silvipastoral model was found to be excellent for fuelwood and forage production and for the amelioration of high pH soils. This system, when followed for little more than four years, reclaims alkali soils to such an extent that normal agriculture crops such as Trifolium alexandrinum and T. resupinatum can be grown successfully. A ridge-trench system of tree planting was found to be helpful in alkali soils, the in situ rainwater conservation it led to assisting biomass production. Eucalyptus tereticornis, Populus deltoides and Tectona grandis based agroforestry were promising for reclaimed salt-affected lands. The short- and long-term effects of various tree plantations on the physicochemical properties of the soil and on soil-water relations are also discussed. Agroforestry options for the development of salt-affected lands found in various agroclimatic zones of India are explored.     

Future of land use in Australia: An economic perspective

It is proposed that the standard economist's model of optimum land use is extended to include the interaction with land cover and land condition. Such a model allows consideration of the influence of market factors and government policies and programmes on land use patterns and management practices, and the feedback effect on land cover and land condition. Recent developments in the approach to modelling land condition, which include the joint consideration of economic factors and physical processes, are discussed. Factors affecting the pattern of agricultural land use in Australia are then reviewed. The importance of considering economic and physical interactions when assessing land use patterns is increasingly being recognized in research and policy development. If research is to meet the needs of land managers in the future then economists and physical scientists will need to integrate their data modelling capabilities in order to address natural resource management issues.