Carrier rate of the c.235G>C mutation in the bovine isoleucyl-tRNA synthetase (IARS) gene of Japanese Black cows at Kagoshima prefecture, Japan,and analysis of the metabolic profiling and reproductive performance of heterozygous cows

Bovine isoleucyl-tRNA synthetase (IARS) disorder, a major cause of weak calf syndrome, is caused by a homozygous missense (c.235G>C) mutation in the bovine IARS gene of Japanese Black (JB) cattle, which was identified in 2013. However, the extent to which the carrier rate has changed at Kagoshima prefecture, Japan, and whether the carrier status is associated with any clinical or reproductive problems, have yet to be ascertained. In this study, using a real-time polymerase chain reaction-based genotyping assay, we determined the carrier rate in a regional JB cow population at Kagoshima prefecture. Comparative analyses were performed on the metabolic profile test (MPT) results and reproductive performance data obtained for heterozygous carrier and homozygous wild-type cows. In 2009 and 2018, DNA samples were collected from 130 and 462 clinically healthy JB cows, respectively, in Kagoshima prefecture. MPT results and reproductive performance data were evaluated for 62 cows, comprising four heterozygous carriers and 58 wild-type cows. Genotyping revealed that the carrier rate was 6.9% in 2009 and 1.5% in 2018, the difference of which was statistically significant (P<0.005). There were no statistically significant differences between the carrier and wild-type cows with respect to either MPT results or reproductive performance, indicating that the carrier cows have necessary IARS activity to maintain minimal health and reproductive potential.     

Fecal carriage of multi-drug resistant and extended spectrum β-lactamases producing E. coli in household pigeons,Bangladesh

Antibiotic resistance and ESBL constitute a risk to human and animal health. Birds residing close to humans could mirror the spectrum of human associated antibiotic resistance. Household pigeons were screened in Bangladesh to shed light on human associated, as well as, environmental antibiotic resistance. Escherichia coli from pigeons (n = 150) were tested against 11 antibiotics. 89% E. coli isolates were resistant to one or more critically important human antibiotics like ampicillin, cefadroxil, mecillinam, ciprofloxacin, gentamicin and tigecycline. No carbapenamase-producers were detected and the lower ESBL prevalence (5%) in pigeons. ESBL-producing E. coli isolates had bla_CTX-M-15 genes. Pigeons shared some bacterial clones and had bird associated sequence types like E. coli ST1408. Fecal carriage of bacteria resistance of critically important human antibiotics, together with examples of shared genotypes among pigeons, indicate the human-birds and bird to bird transmissions are important in the epidemiology of antibiotic resistance.     

Effect of intermittent heat treatment on crystallinity in wood cellulose

The effect of intermittent heat treatment on cellulose crystallites in wood was studied to evaluate quantitatively the changes of crystallinity induced by intermittent and continuous heating. The changes in crystallinity were found to be strongly affected by the intermittent heat treatment. The increased crystallinity, the width of the crystals, and the piezoelectric properties were the same for the first intermittent heating as for continuous heating. Further intermittent heating for the same time duration and temperature had no effect on the above properties, probably due to the stopping of the thermal reaction during the interval. Our results suggested that intermittent heat treatment has certain critical cooling temperatures that stop the thermal reaction and are closely related to the duration of the interval. Samples once exposed to a certain duration of heat treatment and then cooled need more time, about twice that of the first duration of intermittent heating compared with continuous heating, to reach maximum crystallinity in wood cellulose.     

Compressive deformation of wood impregnated with low molecular weight phenol formaldehyde (PF) resin II: effects of processing parameters

To obtain high-strength phenol formaldehyde (PF) resin-impregnated compressed wood at low pressing pressure, the effects of resin content, preheating temperature, pressing temperature, and pressing speed on the compressive deformation of oven-dried low molecular weight PF resin-impregnated wood was investigated. With an increase of PF resin content, the Youngs modulus of the cell wall perpendicular to the fiber direction decreases, and collapse-initiating pressure decreases linearly with the Youngs modulus. This indicates that the occurrence of cell wall collapse is strain-dependent. By increasing preheating temperatures, the collapse-initiating pressure increases due to the increment of the Youngs modulus of the cell wall. An increase in pressing temperature results in the thermal softening of the cell wall and causes collapse at a lower pressure. The wood is compressed effectively despite accelerated resin curing. The pressing speed significantly affects the viscoelastic deformation of the cell wall and the wood is well deformed with decreasing pressing speed, although the differences in density and mechanical properties are relatively small after a pressure-holding period of 30min. In all the parameters examined in this study, the Youngs modulus and bending strength increase with increasing density.     

银合欢落叶层对苗圃生长的森林一农田作物的他感作用(英文)

利用随机区组试验评估了孟加拉国吉大港大学苗圃内的银合欢落叶对森林作物种白格、黄金檀和农田作物种长豇豆、鹰嘴豆和木豆的他感作用。结果表明,凋落叶诱导抑制作物的萌发和生长,该抑制作用依赖于萃取物浓度、凋落物及受体物种类型。萃取物浓度越高则他感作用越强。受体植物的生长响应随应用的凋落叶而变化。低浓度(10g·m-2)凋落叶对农田作物嫩芽生长有促进作用,而其他浓度的凋落叶则呈现明显的抑制作用,施用的凋落叶浓度越大抑制作用也越大。但是不同处理所表现出的抑制趋势并不均一,根系生长受到的影响大于嫩芽。图3表4参34。     

The incidence of canine haematozoa in peninsular Malaysia

In 3 urban areas in Selangor, Peninsular Malaysia between 1973 and 1981, blood from 4080 dogs was examined for haematozoa. The following frequencies were found: Babesia gibsoni 17.7%; microfilariae of Dirofilaria immitis 9.6%; Hepatozoon canis 1.2%; B. canis 1.1%; Ehrlichia canis 0.2%; Trypanosoma evansi 0.1%. A detailed examination of B. gibsoni infections and microfilariasis due to D. immitis with regards to monthly distribution, breed frequency, sex and age, revealed that pedigree and non-pedigree dogs were equally susceptible to Babesia and microfilariae infections.     

Antimicrobial activity of Securinega leucopyrus

Extracts of the leaves of Securinega leucopyrus exhibited a broad spectrum of antimicrobial activity.     

孟加拉涝原地区村林居住的宗教社团偏爱的乔木和灌木树种及使用的造林材料研究

2002年1月至6月对孟加拉涝原地区两个不同的宗教部落(印度教和穆斯林教)的传统家园森林进行了探索性研究，包括印度教和穆斯林教社团对树种(乔木和灌木)的偏爱度、类似性、造林材料及来源、植树地点和间距。研究发现Mangifera indica作为乔木树种，Ocimum sanctum作为灌木树种为印度教社团最喜爱的树种；Artocarpus heterophyllus和Lawsonia inermis分别为穆斯林社团最喜爱乔木树种和灌木树种。两中不同宗教社团选择乔木数种的相同性为86．57％，灌木树种为78．48％。两个宗教部落使用种子和苗木作为造林材料最多。乔木树种的造林材料主要来自家园(39％)灌木树种主要来自自然界(40％)。表3参27。     

棕榈在孟加拉乡村传统利用——一种可持续的生物多样性管理手段(英文)

采用多级采样的方法研究了孟加拉国农村耕作区内棕榈树的传统利用模式和当地的管理经验。农民管理棕榈主要为获取树液产品,生产以糖为主的次生品。树液可直接用作饮料,也可加工成糖蜜或酒精饮料。7块不同地块为棕榈提供生长环境,20.40%的棕榈种植在果园内。尽管中等级的农民拥有多数棕榈(33%),但是主要管理棕榈的是没有土地的农民,他们靠棕榈维持生计。这些农民将自己的智慧应用到棕榈管理当中,从种植到开孔取树液再到产品加工。如果能更科学地管理棕榈,加上农民的智慧,棕榈业将会对当地经济发展起到重要作用,也会丰富当地生物多样性。     

Assessment of natural regeneration status and diversity of tree species in the biodiversity conservation areas of Northeastern Bangladesh

A study was conducted at two-biodiversity conservation areas of Northeastern Bangladesh (a part of Sylhet Forest Division) to assess the species composition, diversity and density of natural regeneration of tree species both indigenous and exotic species. Data were collected by stratified random quadrate method during January 2010 to July 2010. Totally 200 circular plots of 2 m×2 m in size had 5 different habitat types of plants namely; forest, roadside, homestead (surrounding forest dwellers house), fallow land and others (canals, streams and tea gardens side), which included a total of 55 regenerating species belonging to 28 families. Meliaceae is the dominant family and shows the highest family importance value (26.3), having six species, followed by Moraceae (24.24). Among the five habitat types, forest (43 species) possess the highest number of species, followed by roadside (23 species). Total 15 exotic species among 9 families and 40 indigenous species with 24 families were recorded. For exotic species, Tectona grandis possess the highest relative density (11.7%) and relative frequency (10.5%); Senna siamea had highest relative abundance (7.83%). In case of indigenous species, Chickrassia tabularis possess the highest relative density (4.23%) and relative frequency (4%); Dipterocarpus turbinatus had the highest relative abundance (3.92%). Tectona grandis (29.66) and Chickrassia tabularis (10.8) had the highest IVI for exotic and indigenous species respectively. Different diversity indices such as Shanon-Winner diversity index, species diversity index, species richness index, species evenness index, Simpson index and species dominance index, etc. were applied to quantify definite diversity. The regeneration of species associated with low levels of disturbance was in the exotic species. Study suggests that proper protection from human disturbances and scientific management of natural regeneration of two-study forests may lead a rich biodiversity site in the country.