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排序方式: 共有160条查询结果,搜索用时 31 毫秒
51.
Wu CM Chen ZW Chen TH Liao JW Lin CC Chien MS Lee WC Hsuan SL 《Veterinary microbiology》2011,151(3-4):372-378
Actinobacillus pleuropneumoniae exotoxins (Apx) are major virulence factors that play important roles in the pathogenesis of pleuropneumonia in swine. A previous study has demonstrated that native ApxI at low concentrations induces apoptosis in primary porcine alveolar macrophages (PAMs) via a caspase-3-dependent pathway. However, the molecular mechanisms underlying ApxI-induced apoptosis remain largely unknown. In this study, it was shown that ApxI treatment in PAMs rapidly induced phosphorylation of both p38 and JNK, members of the mitogen-activated protein kinase family. Application of a selective p38 or JNK inhibitor significantly reduced ApxI-induced apoptosis, indicating the involvement of p38 and JNK pathways in this event. Furthermore, activation of both caspase-8 and -9 were observed in ApxI-stimulated PAMs. Inhibition of caspase-8 and caspase-9 activity significantly protected PAMs from ApxI-induced apoptosis. In addition, Bid activation was also noted in ApxI-treated PAMs, and inhibition of caspase-8 suppressed the activation of Bid and caspase-9, suggesting that ApxI was able to activate the caspases-8-Bid-caspase-9 pathway. Notably, inhibition of p38 or JNK pathway greatly attenuated the activation of caspases-3, -8, and -9. This study is the first to demonstrate that ApxI-induced apoptosis of PAMs involves the activation of p38 and JNK, and engages the extrinsic and intrinsic apoptotic pathways. 相似文献
52.
Chen HH Chiang W Chang JY Chien YL Lee CK Liu KJ Cheng YT Chen TF Kuo YH Kuo CC 《Journal of agricultural and food chemistry》2011,59(12):6444-6452
Adlay has long been used in traditional Chinese medicine and as a nourishing food. The acetone extract of adlay hull had previously been demonstrated to possess potent antimutagenic activity. The aims of this study were to identify the antimutagenic constituents from adlay hull by using Ames antimutagenic activity-guide isolation procedures and to investigate their chemopreventive efficacies in cultured cells. The results demonstrated that six compounds showing great antimutagenic activity were identified by spectroscopic methods and by comparison with authentic samples to be p-hydroxybenzaldehyde, vanillin, syringaldehyde, trans-coniferylaldehyde, sinapaldehyde, and coixol. Two of them, trans-coniferylaldehyde and sinapaldehyde, exhibit relatively potent scavenging of DPPH radicals, inhibit TPA stimulated superoxide anion generation in neutrophil-like leukocytes, and induce Nrf2/ARE-driven luciferase activity in HSC-3 cells. Moreover, trans-coniferylaldehyde possesses cytoprotective efficacy against tert-butyl hydroperoxide-induced DNA double-strand breaks in cultured cells, and the chemopreventive potency induced by trans-coniferylaldehyde may be through the activation of kinase signals, including p38, ERK1/2, JNK, MEK1/2, and MSK1/2. In summary, we first identified six antimutagenic constituents from adlay hull. Among them, trans-coniferylaldehyde would be a highly promising agent for cancer chemoprevention and merits further investigation. 相似文献
53.
54.
A participatory planning process was applied to develop a media campaign to motivate rice farmers in the Mekong Delta to modify pest management practices together with seed and fertilizer inputs. Locally named ‘Ba Giam Ba Tang’ or ‘Three Reductions, Three Gains’, campaigns were launched in two provinces, Can Tho and Tien Giang. In both provinces, farmers' practices changed significantly. Their insecticide sprays reduced by 13–33% while their seed rates dropped ~10% and nitrogen rates, ~7% and proportion of farmers using insecticides declined by ~11%. These practices were supported by modifications in belief attitudes that favored high inputs. Farmers who reported significant reductions in the three inputs also changed their perception of yield loss. The campaigns in Can Tho and Tien Giang had significant multiplier effects. They stimulated several provincial governments as well as the Ministry of Agriculture and Rural Development to provide additional resources to reproduce the materials and campaign process for local use which eventually reached more than 3 million farmers in south and central Vietnam. 相似文献
55.
In Taiwan, agricultural water is frequently reduced or suspended for reallocation of water resources during drought. To cope with such a situation, the irrigation district of the Shihmen Reservoir in the northern Taiwan fully utilizes the functions of water ponds to regulate water resources, so that industrial and domestic water demands can be met. Constructing new water ponds is indeed able to increase water storage for irrigation. However, when it is compared with the method of dredging or excavating the existing water ponds, constructing new water ponds is not easily accepted by the public because of environmental protection and land acquisition. Dredging water ponds is adopted to obtain a larger storage capacity, so that more flexibility may be acquired in water allocation during drought. Furthermore, it is more practical from engineering point of view. This study presents a formulation of the pond-dredging problem for an irrigation system. The formulation is implemented as optimization programs and applied to the area irrigated by the eighth lateral of the Taoyuan Irrigation Association. The irrigation area is a paddy field of 2,283.8 hectares and consists of 32 water ponds storage capacity of which is analyzed as dredging is conducted. Two models are presented; the first minimizes the water extracted from the Reservoir in a cropping season; while the second maximizes agricultural financial net benefits. Various allowable dredging ratios are used in the two models for different case studies. The first model shows that the water supplied by the Reservoir decreases as the ratio increases, and it reaches the minimum (2,520.3?×?104m3) when the ratio is 0.5. The second model displays that the maximum agricultural financial net benefit increases as the ratio increases and it reaches the highest value (NT$ 6,996?×?104) when the ratio is 0.5, a result similar to that of the first model. However, the dredging priorities of 32 water ponds for the two models are different because of the optimization objectives. The simulation results provide references to the performance improvement of water ponds. With such improvement, water ponds can possess an optimal capacity, and an optimal regional water allocation during drought can be achieved. 相似文献
56.
Shu‐Yun Chen Szu‐Chien Liu Tzu‐Chuan Lo Andi Wahyono Sz‐Yun Chen Hsin‐Mei Ku 《Plant Breeding》2014,133(2):291-297
Non‐pungent bell pepper (Capsicum annuum L.) lacks the cytoplasmic male sterility (CMS) nuclear restorer allele, Rf, and CMS cannot be employed in its F1 hybrid seed production. To demonstrate that the genic male sterility (GMS) system in non‐pungent bell pepper can be converted to the CMS male sterility system, the conversion of GMS to CMS for non‐pungent bell pepper line GC3 was conducted by introgression of S‐type cytoplasm and the Rf allele from tropical pungent donors. After morphological traits were evaluated, two lines from BC1F1 containing S‐type cytoplasm and four lines from BC2F2 containing Rf allele, phenotypically similar to GC3, were obtained and could be employed as CMS male sterile lines and restorer lines for non‐pungent bell pepper. Four molecular markers potentially linked to traits of interest were also evaluated in BC1F1 and BC1F2 populations. This is the first time that GMS has been successfully converted to CMS in bell pepper, a significant contribution for bell pepper hybrid seed production. 相似文献
57.
Jyh-Horng Wu Yu-Tang Tung Chiou-Fung Chyu Shih-Chang Chien Sheng-Yang Wang Shang-Tzen Chang Yueh-Hsiung Kuo 《Journal of Wood Science》2008,54(5):383-389
The methanolic extract of the root of Garcinia multiflora and its derived soluble fractions, namely those soluble in ethyl acetate (EtOAc), n-butanol, and water, were screened for their antioxidant activities. Among them, the EtOAc-soluble fraction exhibited the
highest scavenging activity against the 1,1-dipheny1,2-picrylhydrazyl radical, the highest superoxide radical scavenging activity,
and the strongest reducing power. In addition, according to the bioactivity-guided isolation, 26 specific phytocompounds,
including 3 aromatics, 3 benzophenones, 3 flavonoids, 3 isocoumarins, 1 phloroglucinol, 6 steroids, and 7 xanthones, were
isolated from the EtOAc-soluble fraction and identified. Of these, 2,4,3′,4′-tetrahydroxy-6-methoxybenzophenone and 1,3,6,7-tetrahydroxyxanthone
were found to be the major bioactive constituents, present in the crude extract in concentrations of 3.9 and 15.6 mg/g, respectively.
These two compounds had similar antioxidant activities to (+)-catechin, a well-known antioxidant. 相似文献
58.
H. P. Ritzema Le Dinh Thinh Le Quang Anh Dang Ngoc Hanh Nguyen Viet Chien Trinh Ngoc Lan R. A. L. Kselik Bui Thi Kim 《Irrigation and Drainage Systems》2008,22(1):19-34
The irrigation and drainage systems in the Red River Delta in Vietnam were designed and constructed in the 1950s and 60s.
These systems are well established and provide water to virtually all of the irrigable land in the Delta. The land is cropped
intensively: on average just over two crops a year. The irrigation and drainage systems are complex: dual purpose channels
and pumping stations are used. In the 1990s, the systems were rehabilitated and upgraded. A review showed that irrigation
projects performed reasonably well, but the two core drainage projects performed less than anticipated. In the Red River Delta,
with its low elevations, drainage rather than irrigation is often the limiting factor affecting agricultural production. To
investigate these constraints in more detail, a participatory research study on the effectiveness of drainage was conducted
in two drainage areas in the Red River Delta. The study started with a participatory pre-investigation to identify and quantify
the constraints in the functioning of the drainage systems. Next, the drainage system was modelled and computer simulations
were used to develop conceptual designs to improve the functioning of the systems. Finally, recommendations to improve the
institutional capacity of the drainage system management were formulated, again in close cooperation with the stakeholders.
These recommendations cover a whole range of technical issues: small-scale improvements in the farmers’ fields, modifications
in the main and secondary drainage systems, including the control structures, up to recommendations to increase the efficiency
of the pumping stations. Next to these technical innovations, recommendations to reform the complex institutional setting
have been formulated. 相似文献
59.
Ching-Chu Tsai Li-Fen Hung Jeng-Der Chung Shiang-Jiuun Chen Ching-Te Chien Wen-Yuan Kao Ling-Long Kuo-Huang 《Annals of Forest Science》2018,75(2):52
Key message
The radial wood growth curves of Cinnamomum kanehirae Hayata (an endangered species of subtropical Taiwan) exhibit an S shape. The dominant trees displayed a larger radial growth than the codominant trees, and their growth was more sensitive to air temperature.Context
Knowledge of wood radial growth is important for evaluating the factors that limit tree growth performance. The relevant experiments have mostly been conducted in cold and temperate ecosystems, but rarely in subtropical ecosystems.Aims
In this study, we aimed to construct a unified radial growth model for Cinnamomum kanehirae Hayata and to identify its sensitivity to temperature.Methods
The wood radial increments were quantified for 3 years by either pinning or microcoring. The radial wood growth curves were modelled integratively by semiparametric regression and individually by curve fitting. The effects of tree social class, interannual and environmental factors on radial growth were analysed quantitatively.Results
A unified S-shaped growth model for C. kanehirae was successfully constructed. By including the social class effect, the model was significantly improved. The maximum radial increment (A) was significantly correlated with the maximum growth rate (μ); both A and μ were significantly higher in dominant than in codominant trees. The time-varying radial growth rate was more sensitive to air temperature in dominant than in codominant trees.Conclusion
Semiparametric models revealed an S-shaped growth curve of C. kanehirae and confirmed the higher temperature sensitivity of dominant trees compared to codominant trees in humid subtropical areas.60.
Chien EY Liu W Zhao Q Katritch V Han GW Hanson MA Shi L Newman AH Javitch JA Cherezov V Stevens RC 《Science (New York, N.Y.)》2010,330(6007):1091-1095
Dopamine modulates movement, cognition, and emotion through activation of dopamine G protein-coupled receptors in the brain. The crystal structure of the human dopamine D3 receptor (D3R) in complex with the small molecule D2R/D3R-specific antagonist eticlopride reveals important features of the ligand binding pocket and extracellular loops. On the intracellular side of the receptor, a locked conformation of the ionic lock and two distinctly different conformations of intracellular loop 2 are observed. Docking of R-22, a D3R-selective antagonist, reveals an extracellular extension of the eticlopride binding site that comprises a second binding pocket for the aryl amide of R-22, which differs between the highly homologous D2R and D3R. This difference provides direction to the design of D3R-selective agents for treating drug abuse and other neuropsychiatric indications. 相似文献