Evaluation of biofloc technology in pink shrimp Farfantepenaeus duorarum culture: growth performance, water quality, microorganisms profile and proximate analysis of biofloc

In a 210d experiment, the potential of biofloc technology (BFT) was evaluated for Farfantepenaeus duorarum. Water quality parameters, microorganisms profile and proximate analysis of biofloc were also assessed. BFT did not improve the growth performance in F. duorarum when compared to conventional clear-water water exchange system (final weight and survival of 13.3 g and 63.2 %; and 13.9 g and 81.4 %, respectively). Microorganism assessment suggested a higher presence of filamentous cyanobacteria followed by protozoa, nematodes and copepods. Proximate analysis of biofloc showed crude protein and crude lipid means levels of 25 and 0.6 %, respectively, and these values varied during the experiment. F. duorarum seemed to be susceptible to high stocking density and high levels of suspended solids (>15 mL L^?1).     

Effect of short-term fresh food supplementation on reproductive performance, biochemical composition, and fatty acid profile of Litopenaeus vannamei (Boone) reared under biofloc conditions

A 40-day trial was performed to evaluate the effect of short-term natural fresh food (FF) supplementation 20 days prior to ablation in Litopenaeus vannamei broodstock raised under biofloc conditions. Changes in biochemical composition and fatty acids profile were used as indicators of nutritional condition. Females that received FF supplementation (FLOC + FF) achieved better eggs production, spawned more promptly, and presented higher levels of HUFA in eggs as compared to those that did not received FF (FLOC). Proximate analysis of biofloc and microorganisms assessment showed a higher crude protein and lipid content in the FLOC + FF treatment (26.3 and 0.7 %) compared to FLOC treatment (18.4 and 0.3 %) and also demonstrated a higher concentration of filamentous cyanobacteria and nematodes. The better outcomes obtained in females that received short-term FF supplementation justified the FF use in L. vannamei broodstock.     

Mapping QTLs for Witches' Broom (Crinipellis Perniciosa) Resistance in Cacao (Theobroma Cacao L.)

Molecular markers (RAPD, AFLP and microsatellites) were used to generate a linkage map and to identify QTLs associated to witches' broom (Crinipellis perniciosa) resistance in cacao (Theobroma cacao), using 82 individuals of an F₂ population derived from the clones ICS-1 (susceptible) and Scavina-6 (resistant). Fifteen evaluations of the number of brooms have been carried out in six years (1997–2002). In order to increase the precision and accuracy in the measures of resistance, each F₂ plant was cloned in three replications in a randomized block design with single-tree plots and evaluated over 2 years. Three hundred and forty-two markers were obtained, being 33 microsatellites, 77 AFLPs and 232 RAPDs. The distribution of the number of brooms in the F₂ population was skewed to resistance, suggesting the involvement of major genes controlling resistance and the repeatability estimated for resistance was 44%. A strong putative QTL was detected as being related to witches' broom resistance. Associated to this QTL, the microsatellite mTcCIR35 explained 35.5% of the phenotypic variation in resistance. This marker is being used for marker-assisted selection in Scavina-6 progenies, including those selected in private plantations, as an auxiliary tool to the phenotypic selection.     

Organic residues and their impact on soil health,crop production and sustainable agriculture: A review including bibliographic analysis

Despite their potential as a sustainable source of soil nutrients and organic matter, waste organic residues are often discarded rather than utilized in agriculture. This review examines the current state-of-the-art in the application of organic residues in agricultural production systems by analysing trends and results in published research conducted on their use for soil health and crop production purposes. For this, a bibliographic analysis was conducted on 81 papers collected from the Web of Science database. Our research shows a growing trend in the field, particularly in recent years, with articles from India, China and the United States at the forefront. The most commonly evaluated organic residues were compost, animal manure, crop residues, municipal solid waste and biochar. Soil pH, bulk density and especially organic matter/total organic carbon (TOC) were the chemical, physical and biological attributes most frequently evaluated, respectively. This review indicates that the application of organic residues improves soil health, positively affecting crop productivity in crops such as maize, wheat and rice. However, further research is needed to address the potential risk of soil contamination from potentially toxic metals (PTMs) associated with organic residues, as well as to identify best practices to guarantee food safety and environmental preservation.     

Microbiological Quality and Antibiotic Resistance Analysis of a Brazilian Water Supply Source

Water samples of the Passaúna River, Curitiba/Paraná (Brazil), were analyzed to determine total and thermotolerant coliform counts and Escherichia coli in order to provide information on human impacts on the water supply. Samples were collected and analyzed monthly, from March 2006 to February 2007, at five different locations along the river, and the multiple tube method was used to obtain total and thermotolerant coliform counts. The results varied from 130 MPN/100 mL to 1.6?×?10⁶ MPN/100 mL for total coliforms, while for thermotolerant coliforms the variation was between 40 MPN/100 mL and 5?×?10⁵ MPN/100 mL. The E. coli strains isolated from the samples were tested with 13 different antibiotics to determine their antibiotic resistance. The isolated strains were constantly sensitive to seven of the 13 antibiotics tested, and resistant to at least one of the other antibiotics. The results indicated that two factors could influence the increased contamination on this river, viz., seasonality parameters and domestic wastewater discharges. The determination of antibiotic resistance indices aimed to provide information on the anthropogenic influence. Only one of the locations investigated was considered critical due to the anthropogenic influence, with significant impacts from irregular domestic wastewater discharges.     

Laparoscopic-assisted incisional colopexy by two portals access in a domestic cat with recurrent rectal prolapse

A 2-year-old female mixed-breed cat weighing 2.7 kg presented with recurrent rectal prolapse. The following report describes its treatment by laparoscopic-assisted incisional colopexy using two portals. The procedure was effective and without trans- or postoperative complications. Recurrent prolapses did not recur in the 2 months of postoperative observation.     

Clinical and parasitological evaluation of dogs naturally infected by Leishmania (Leishmania) chagasi submitted to treatment with meglumine antimoniate

Aiming to evaluate the efficacy of the treatment of canine visceral leishmaniasis, to verify the occurrence of a possible disease relapse, and to search for the presence of the parasites after the end of the treatment, seven dogs naturally infected by Leishmania (Leishmania) chagasi were used. The dogs were subjected to a treatment with 75 mg/kg meglumine antimoniate subcutaneously every 12 h for 21 days, and followed-up for a period of 6 months. During the whole experimental period the animals wore deltamethrin collars and were kept in a screened kennel to avoid reinfection. Lymph node and bone marrow aspiration biopsy was carried out to search for the parasite at seven moments: before the treatment, 30, 60, 90, 120, 150 and 180 days after the start of the treatment. After the end of the experiment all dogs were humanely euthanized. Then, spleen and liver "imprints" and in vitro cultures were carried out to search for amastigote forms of the parasite. During the treatment all animals presented remission of symptoms. However, two dogs were observed to present new symptoms in the course of the experiment. At the end of the experiment, the presence of amastigote forms of the parasite was evidenced in five of the seven dogs. This enabled us to conclude that the treatment promoted clinical cure but did not eliminate the parasites completely.     

Enhanced susceptibility of channel catfish to the bacterium Edwardsiella ictaluri after parasitism by Ichthyophthirius multifiliis

Bacterium Edwardsiella ictaluri and parasite Ichthyophthirius multifiliis (Ich) are two common pathogens of cultured fish. The objective of this study was to evaluate the susceptibility of channel catfish Ictalurus punctatus to E. ictaluri and determine bacterial loads in different fish organs after parasitism by Ich. Fish received the following treatments: (1) infected by I. multifiliis at 5000 theronts/fish and exposed to E. ictaluri; (2) infected by I. multifiliis alone; (3) exposed to E. ictaluri alone; and (4) non-infected control. E. ictaluri in fish organs were quantified by quantitative real-time polymerase chain reaction and reported as genome equivalents per mg of tissue (GEs/mg). The results demonstrated that the Ich-parasitized catfish showed significantly (P<0.05) higher mortality (91.7%) when exposed to E. ictaluri than non-parasitized fish (10%). The bacterial loads in fish infected by 5000 theronts/fish ranged from 6497 to 163,898 GEs/mg which was between 40 and 2000 fold higher than non-parasitized fish (49-141 GEs/mg). Ich infection enhanced the susceptibility of channel catfish to bacterial invasion and increased fish mortality.     

Protein supplementation to early lactation dairy cows grazing tropical grass: Performance and ruminal metabolism

This experiment was designed to evaluate the effects of different concentrate crude protein (CP) concentration on performance, metabolism and efficiency of N utilization (ENU) on early-lactation dairy cows grazing intensively managed tropical grass. Thirty cows were used in a ten replicated 3 × 3 Latin square design. The treatments consisted of three levels of concentrate CP: 7.9%, 15.4%, and 20.5% offered at a rate of 1 kg (as-fed basis)/3 kg of milk. The cows fed low and medium CP had negative balance of rumen degradable protein and metabolizable protein. Increasing CP tended to linearly increase DMI, 3.5% FCM and milk casein, and linearly increased the yields of milk fat and protein. Increasing CP linearly increased the intake of N, the concentration of rumen NH₃–N, and the losses of N in milk, urine, and feces. Increasing dietary CP linearly increased the molar proportion of butyrate but had no effect on the other rumen VFAs and no effect on microbial yield. In conclusion, feeding a concentrate with 20.5% of CP to early-lactation dairy cows grazing tropical grasses, leading to a 17.8% CP diet, tended to increase DMI, increased the yield of 3.5% FCM and the milk N excretion, and decreased ENU by 32%.