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971.
Pertussis toxin S1 mutant with reduced enzyme activity and a conserved protective epitope 总被引:28,自引:0,他引:28
W N Burnette W Cieplak V L Mar K T Kaljot H Sato J M Keith 《Science (New York, N.Y.)》1988,242(4875):72-74
Pertussis toxin (PTX) is a major virulence factor in whooping cough and can elicit protective antibodies. Amino acid residues 8 to 15 of PTX subunit S1 are important for the adenosine diphosphate-ribosyltransferase activity associated with the pathobiological effects of PTX. Furthermore, this region contains at least a portion of an epitope that elicits both toxin-neutralizing and protective antibody responses in mice. The gene encoding the S1 subunit was subjected to site-specific mutagenesis in this critical region. A mutant containing a single amino acid substitution (Arg9----Lys) had reduced enzymatic activity (approximately 0.02% of control) while retaining the protective epitope. This analog S1 molecule may provide the basis for a genetically detoxified PTX with potential for use as a component of an acellular vaccine against whooping cough. 相似文献
972.
Hernández PA Notsu K Salazar JM Mori T Natale G Okada H Virgili G Shimoike Y Sato M Pérez NM 《Science (New York, N.Y.)》2001,292(5514):83-86
Magmatic carbon dioxide (CO2) degassing has been documented before the 31 March 2000 eruption of Usu volcano, Hokkaido, Japan. Six months before the eruption, an increase in CO2 flux was detected on the summit caldera, from 120 (September 1998) to 340 metric tons per day (September 1999), followed by a sudden decrease to 39 metric tons per day in June 2000, 3 months after the eruption. The change in CO2 flux and seismic observations suggests that before the eruption, advective processes controlled gas migration toward the surface. The decrease in flux after the eruption at the summit caldera could be due to a rapid release of CO2 during the eruption from ascending dacitic dikes spreading away from the magma chamber beneath the caldera. 相似文献
973.
Diana Stuart 《Agriculture and Human Values》2008,25(2):177-181
I explore the role of nature in the agrifood system and how attempts to fit food production into a large-scale manufacturing
model has lead to widespread outbreaks of food borne illness. I illustrate how industrial processing of leafy greens is related
to the outbreak of E. coli 0157:H7 associated with spinach in the fall of 2006. I also use this example to show how industry attempts to create the
illusion of control while failing to address weaknesses in current processing systems. The leafy greens industry has focused
efforts on sterilizing the growing environment and adopting new technologies, while neglecting to change the concentrated
structure of processing systems. Repeated breakdowns in these systems illustrate a widening fault line between attempted and
failed control of nature in industrial food production.
相似文献
Diana StuartEmail: |
974.
Shahzad Ali Amanullah Jan Jia Zhikuan Amir Sohail Cai Tie Wei Ting Zhang Peng Manzoor Irshad Ahmad Mati Ur Rahman Ren Xiaolong Liu Xiaoli Xu Yue Yue 《Russian Agricultural Sciences》2016,42(3-4):224-229
A field experiment was conducted on silty clay loam soil in the years 2011–2012. Two sesame (Sesamum indicum L.) cultivars (Local Black and Local White) were evaluated using various 3 different sowing dates (20th June, 10th and 30th July) and four agrotechnical level (0, 40, 80 and 120 kg N ha–1) at New Developmental Farm The University of Agriculture, Peshawar, Pakistan. The objective of this study was to evaluate the effects of sowing dates on growth, yield and oil fatty acid composition of two sesame cultivars grown under different nitrogen fertilization. Results showed that cv. Local Black was characterized by significantly higher content of oil (47%), seed yield (696 kg ha–1) and oil yield (335 kg ha–1) while cv. Local White had higher palmitic acid (8%) and linoleic acid (38.7%). Yield and its main components were positively affected by the earlier sowing date. With regard to fatty acid composition, a decrease in oleic and stearic acid and an increase in linoleic and palmitic acid were observed. At early sowing, oleic and palmitic acid decreased whereas linoleic and stearic acid increased. The decrease in the oleic/linoleic acid ratio observed at early sowing, suggests a possible role of temperature on the activity of oleate desaturase in the developing seeds. Intensive technology of cultivation (120 kg N ha–1), compared to the economical technology (40 kg N ha–1), significantly increased the seed yield of both sesame cultivars. This was due to higher number of branches, Capsules m–2, capsules plant–1, seeds capsule–1 and 1000 seed weight (g). The intensive technology of cultivation had a beneficial effect on the content of palmitic acid, linolenic acid and oleic acid in sesame seed. 相似文献
975.
An electrochemical device was used to measure the fugacity of oxygen (fo(o2)) in holes drilled through the crust of Makaopuhi lava lake, Kilauea Volcano, Hawaii. Results obtained within 6 months of the lake formation show that log fo(o2) normally varies linearly with the reciprocal of the absolute temperature, and that chemical changes occurring in the cooling tholeiitic basalt are reflected in the fo(o2) values measured in the holes. 相似文献
976.
Wei Feng Wenying Shen Li He Jianzhao Duan Binbin Guo Yingxue Li Chenyang Wang Tiancai Guo 《Precision Agriculture》2016,17(5):608-627
In this study, we investigated the possibility of using ground-based remote sensing technology to estimate powdery mildew disease severity in winter wheat. Using artificially inoculated fields, potted plants, and disease nursery tests, we measured the powdery mildew canopy spectra of varieties of wheat at different levels of incidence and growth stages to investigate the disease severity. The results showed that the powdery mildew sensitive bands were between 580 and 710 nm. The best two-band vegetation index that correlated with wheat powdery mildew between 400 and 1000 nm wavelength were the normalized spectrum 570–590 and 536–566 nm bands for the ratio index, and 568–592 and 528–570 nm for the normalized difference index. The coefficients of determination (R 2) for both were almost the same. The optimum dual-green vegetation index was constructed based on a calculation of the ratio and normalized difference between the normalized spectrum within the two green bands. The coefficients of determination (R 2) of DGSR (584, 550) (dual-green simple ratio) and DGND (584, 550) (dual-green normalized difference) were both 0.845. The inverse models of disease severity performed well in the test process at the canopy scale, and indicated that, compared with the traditional vegetation indices of Lwidth, mND705, ND (SDr, SDb), SIPI, and GNDVI, the novel dual-green indices greatly improved the remote sensing detection of wheat powdery mildew disease. Following these results, combined disease severity and canopy spectra were shown to be of enormous value when applied to the accurate monitoring, prevention, and control of crop diseases. 相似文献
977.
Induction of inflammatory arthropathy resembling rheumatoid arthritis in mice transgenic for HTLV-I 总被引:28,自引:0,他引:28
Y Iwakura M Tosu E Yoshida M Takiguchi K Sato I Kitajima K Nishioka K Yamamoto T Takeda M Hatanaka 《Science (New York, N.Y.)》1991,253(5023):1026-1028
Human T cell leukemia virus type-I (HTLV-I) is the etiologic agent of adult T cell leukemia and has also been suggested to be involved in other diseases such as chronic arthritis or myelopathy. To elucidate pathological roles of the virus in disease, transgenic mice were produced that carry the HTLV-I genome. At 2 to 3 months of age, many of the mice developed chronic arthritis resembling rheumatoid arthritis. Synovial and periarticular inflammation with articular erosion caused by invasion of granulation tissues were marked. These observations suggest a possibility that HTLV-I is one of the etiologic agents of chronic arthritis in humans. 相似文献
978.
Most accounts of the effect of the global marketplace on deforestation in Africa, Asia, and Latin America emphasize the demand
for timber used in industrial processes and the conversion of tropical forests to pastures for beef cattle. In recent years,
numerous scholars and policymakers have suggested that developing a market for non-timber forest products (NTFPs) might slow
the pace of habitat destruction. Although increased demand for NTFPs rarely results in massive deforestation, the depletion
of the raw materials needed to make particular products is common.
Many rural households in the Mexican state of Oaxaca have prospered over the past three decades through the sale of brightly-painted,
whimsical wood carvings (alebrijes) to international tourists and the owners of ethnic arts shops in the United States, Canada, and Europe. This paper examines
a promising project aimed at providing Oaxacan alebrije-makers with a reliable, legal, and sustainable supply of wood. The
ecologists, artisans, merchants, and forest owners involved in the project face formidable obstacles. Gaining permission to
harvest wood from land belonging to Oaxacan communities requires the negotiation of a complex social, legal, economic, and
political landscape. Artisans’ decisions about where to obtain wood rest largely on price, quality, and reliability of the
supplier; they are willing to pay a premium for ecologically sustainable wood only if the additional cost can be passed on
to consumers. Nonetheless, a group of carvers has begun to buy sustainably harvested wood. This arrangement has economic advantages
for both the alebrije-makers and the owners of the forests where the wood is produced.
Michael
Chibnik is Professor of Anthropology at the University of Iowa. He has conducted fieldwork in Belize, Peru, Mexico, and in various
parts of the United States. His research interests include economic anthropology, artisans, work organization, agricultural
decision-making, and political ecology. He is the author of Crafting Tradition: The Making and Marketing of Oaxacan Wood Carvings
(University of Texas Press, 2003) and Risky Rivers: The Economics and Politics of Floodplain Farming in Amazonia (University of Arizona Press, 1994), and editor of Farm Work and Fieldwork: American Agriculture in Anthropological Perspective (Cornell University Press, 1987).
Dr. Silvia E. Purata is a Mexican ethnoecologist based in Xalapa, Veracruz. She is a member of People and Plants International, an organization
that works to integrate conservation and the use of natural resources. Purata has conducted research on the methods indigenous
peoples use to extract non-timber forest products (NTFPs) in tropical forests and the fate of such systems in varying socioeconomic
circumstances. She has also been working on the promotion of forest certification in the Selva Maya. 相似文献
979.
Stefano Ponte 《Agriculture and Human Values》2007,24(2):179-193
Contemporary regulation of food safety incorporates principles of quality management and systemic performance objectives that
used to characterize private standards. Conversely, private standards are covering ground that used to be the realm of regulation.
The nature of the two is becoming increasingly indistinguishable. The case study of the Ugandan fish export industry highlights
how management methods borrowed from private standards can be applied to public regulation to achieve seemingly conflicting
objectives. In the late 1990s, the EU imposed repeated bans on fish imported from Uganda on the basis of food safety concerns.
However, the EU did not provide scientific proof that the fish were actually “unsafe.” Rather, the poor performance of Uganda’s
regulatory and monitoring system was used as justification. Only by fixing “the system” (of regulations and inspections) and
performing the ritual of laboratory testing for all consignments for export to the EU did the Ugandan industry regain its
status as a “safe” source of fish. Yet, gaps and inconsistencies abound in the current Ugandan fish safety management system.
Some operations are by necessity carried out as “rituals of verification.” Given the importance of microbiological tests and
laboratories in the compliance system, “alchemic rituals” provide an appropriate metaphor. These rituals are part and parcel
of a model that reassures the EU fish-eating public that all is under control in Uganda from boat to point of export. As a
consequence, actual non-compliance from boat to landing site allows the fishery to survive as an artisanal operation.
Stefano Ponte
is Senior Researcher at the Danish Institute for International Studies, Copenhagen. His research focuses on the role of standards,
regulation and quality conventions in the governance of agro-food value chains, with particular focus on Africa. He is co-author
of Trading Down: Africa, Value Chains and the Global Economy and The Coffee Paradox: Global Markets, Commodity Trade and the
Elusive Promise of Development. 相似文献
980.
We examine changing production relations in the Mexican tequila industry to explore the ways in which large industrial firms
are using “reverse leasing arrangements,” a form of contract farming, to extend their control over small agave farmers. Under
these arrangements, smallholders rent their parcels to contracting companies who bring in capital, machinery, labor, and other
agricultural inputs. Smallholders do not have access to their land, nor do they make any of the management decisions. We analyze
the factors that have led some producers to participate in reverse leasing arrangements, while allowing other producers to
continue farming independently. In addition, we look at the ways in which farmers are responding to these new production relations
and constraints and the strategies that they are using to regain control over the production process.
相似文献
Sarah BowenEmail: |