全文获取类型
收费全文 | 2065篇 |
免费 | 121篇 |
国内免费 | 1篇 |
专业分类
林业 | 82篇 |
农学 | 42篇 |
基础科学 | 5篇 |
351篇 | |
综合类 | 295篇 |
农作物 | 106篇 |
水产渔业 | 107篇 |
畜牧兽医 | 1080篇 |
园艺 | 32篇 |
植物保护 | 87篇 |
出版年
2023年 | 18篇 |
2022年 | 18篇 |
2021年 | 32篇 |
2020年 | 45篇 |
2019年 | 54篇 |
2018年 | 47篇 |
2017年 | 44篇 |
2016年 | 53篇 |
2015年 | 37篇 |
2014年 | 60篇 |
2013年 | 94篇 |
2012年 | 119篇 |
2011年 | 188篇 |
2010年 | 73篇 |
2009年 | 69篇 |
2008年 | 140篇 |
2007年 | 122篇 |
2006年 | 116篇 |
2005年 | 144篇 |
2004年 | 143篇 |
2003年 | 115篇 |
2002年 | 115篇 |
2001年 | 30篇 |
2000年 | 17篇 |
1999年 | 11篇 |
1998年 | 15篇 |
1997年 | 15篇 |
1996年 | 14篇 |
1995年 | 17篇 |
1994年 | 21篇 |
1993年 | 10篇 |
1992年 | 17篇 |
1991年 | 15篇 |
1990年 | 10篇 |
1989年 | 5篇 |
1988年 | 5篇 |
1987年 | 9篇 |
1986年 | 9篇 |
1985年 | 10篇 |
1984年 | 8篇 |
1983年 | 8篇 |
1982年 | 7篇 |
1980年 | 6篇 |
1977年 | 5篇 |
1975年 | 5篇 |
1972年 | 6篇 |
1967年 | 5篇 |
1966年 | 7篇 |
1959年 | 4篇 |
1953年 | 5篇 |
排序方式: 共有2187条查询结果,搜索用时 15 毫秒
971.
Arias SL Theumer MG Mary VS Rubinstein HR 《Journal of agricultural and food chemistry》2012,60(22):5667-5675
Fusarium verticillioides is best known for its worldwide occurrence on maize resulting in highly variable disease symptoms, ranging from asymptomatic to severe rotting and wilting and fumonisin production. The aim of this study was to investigate the influence of hybrid genotypes in the early stages of F. verticillioides infection, and the role of fumonisins as effectors in the outcome of this complex interaction. Disease symptoms, growth parameters, root morphology, and fungal colonization were evaluated at 7, 14, and 21 days after planting in seedlings from maize seeds of resistant (RH) and susceptible (SH) hybrids inoculated with F. verticillioides or watered with solutions of fumonisins. F. verticillioides induced growth enhancement or retardation depending on the plant genetic background and the fungal colonization rate, while fumonisins caused severe reduction in biomass and fitness. Seedlings watered with high fumonisin concentrations displayed lesions similar to those seen in F. verticillioides maize seedling disease, and also elicited inhibitory effects on root growth and morphology and on functional properties. In summary, these data strongly suggest a dual role for fumonisins in the F. verticillioides-maize interaction, acting as pathogenic factors at high concentrations, or triggering the plant detoxification mechanisms at low levels. 相似文献
972.
Polavarapu S Oliver CM Ajlouni S Augustin MA 《Journal of agricultural and food chemistry》2012,60(1):444-450
The influence of EDTA on lipid oxidation in sugar beet pectin-stabilized oil-in-water emulsions (pH 6, 15% oil, wet basis), prepared from fish oil (FO) and fish oil-extra virgin olive oil (FO-EVOO) (1:1 w/w), as well as the spray-dried microcapsules (50% oil, dry basis) prepared from these emulsions, was investigated. Under accelerated conditions (80 °C, 5 bar oxygen pressure) the oxidative stability was significantly (P < 0.05) higher for FO and FO-EVOO formulated with EDTA, in comparison to corresponding emulsions and spray-dried microcapsules formulated without EDTA. The EDTA effect was greater in emulsions than in spray-dried microcapsules, with the greatest protective effect obtained in FO-EVOO emulsions. EDTA enhanced the oxidative stability of the spray-dried microcapsules during ambient storage (~25 °C, a(w) = 0.5), as demonstrated by their lower concentration of headspace volatile oxidation products, propanal and hexanal. These results show that the addition of EDTA is an effective strategy to maximize the oxidative stability of both FO emulsions and spray-dried microcapsules in which sugar beet pectin is used as the encapsulant material. 相似文献
973.
974.
Mary Ruth McDonald Bruce D. Gossen Cezarina Kora Monica Parker Greg Boland 《European journal of plant pathology / European Foundation for Plant Pathology》2013,135(3):581-593
Modifying crop canopies can suppress plant diseases in some crops. For example, in carrot, lateral trimming of the canopy by 30–40 % after canopy closure reduced sclerotinia rot (Sclerotinia sclerotiorum) to zero under moderate disease pressure without the use of fungicides. Trimming reduced relative humidity within the carrot canopy and increased air and soil temperature, inhibiting the formation of apothecia of S. sclerotiorum. Trimming also severed infected petioles, which reduced the opportunity for infection to progress to the carrot crown. Trimming combined with application of foliar fungicide was even more effective. Trimming reduced carrot leaf blights (Alternaria dauci, Cercospora carotae) in 1 of 3 years, when disease pressure was low. However, there was no advantage of combining trimming and fungicide sprays for leaf blight control. Canopy modification also reduces disease in legume crops. Soybean cultivars with reduced height and lodging, and early maturity, had up to a 74 % reduction in apothecia of S. sclerotiorum within the crop, and up to an 88 % reduction in disease incidence at harvest. In field pea, artificially supporting plants to reduce lodging, in combination with fungicide application, reduced the severity of mycosphaerella blight (Mycosphaerella pinodes) on pods by 67 % and increased seed yield by 54 %. In chickpea, paired-row planting that opened the canopy increased seed yield by 12 %, likely by increasing fungicide deposition. Modifications of the crop canopy can reduce disease, the need for fungicide sprays, and sometimes improve fungicide efficacy, but the results are often pathosystem-specific. 相似文献
975.
Alejandro M. S. Mayer Mary L. Hall Michael Holland Cristina De Castro Antonio Molinaro Monica Aldulescu Jeffrey Frenkel Lauren Ottenhoff David Rowley Jan Powell 《Marine drugs》2014,12(4):1732-1756
Although human exposure to Gram-negative Vibrio vulnificus (V. vulnificus) lipopolysaccharide (LPS) has been reported to result in septic shock, its impact on the central nervous system’s innate immunity remains undetermined. The purpose of this study was to determine whether V. vulnificus MO6-24/O LPS might activate rat microglia in vitro and stimulate the release of superoxide anion (O2−), a reactive oxygen species known to cause oxidative stress and neuronal injury in vivo. Brain microglia were isolated from neonatal rats, and then treated with either V. vulnificus MO6-24/O LPS or Escherichia coli O26:B6 LPS for 17 hours in vitro. O2− was determined by cytochrome C reduction, and matrix metalloproteinase-2 (MMP-2) and MMP-9 by gelatinase zymography. Generation of cytokines tumor necrosis factor alpha (TNF-α), interleukin-1 alpha (IL-1α), IL-6, and transforming growth factor-beta 1 (TGF-β1), chemokines macrophage inflammatory protein (MIP-1α)/chemokine (C-C motif) ligand 3 (CCL3), MIP-2/chemokine (C-X-C motif) ligand 2 (CXCL2), monocyte chemotactic protein-1 (MCP-1)/CCL2, and cytokine-induced neutrophil chemoattractant-2alpha/beta (CINC-2α/β)/CXCL3, and brain-derived neurotrophic factor (BDNF), were determined by specific immunoassays. Priming of rat microglia by V. vulnificus MO6-24/O LPS in vitro yielded a bell-shaped dose-response curve for PMA (phorbol 12-myristate 13-acetate)-stimulated O2− generation: (1) 0.1–1 ng/mL V. vulnificus LPS enhanced O2− generation significantly but with limited inflammatory mediator generation; (2) 10–100 ng/mL V. vulnificus LPS maximized O2− generation with concomitant release of thromboxane B2 (TXB2), matrix metalloproteinase-9 (MMP-9), and several cytokines and chemokines; (3) 1000–100,000 ng/mL V. vulnificus LPS, with the exception of TXB2, yielded both attenuated O2− production, and a progressive decrease in MMP-9, cytokines and chemokines investigated. Thus concentration-dependent treatment of neonatal brain microglia with V. vulnificus MO6-24/O LPS resulted in a significant rise in O2− production, followed by a progressive decrease in O2− release, with concomitant release of lactic dehydrogenase (LDH), and generation of TXB2, MMP-9, cytokines and chemokines. We hypothesize that the inflammatory mediators investigated may be cytotoxic to microglia in vitro, by an as yet undetermined autocrine mechanism. Although V. vulnificus LPS was less potent than E. coli LPS in vitro, inflammatory mediator release by the former was clearly more efficacious. Finally, we hypothesize that should V. vulnificus LPS gain entry into the CNS, it would be possible that microglia might become activated, resulting in high levels of O2− as well as neuroinflammatory TXB2, MMP-9, cytokines and chemokines. 相似文献
976.
Global incidence of type 2 diabetes has escalated over the past few decades, necessitating a continued search for natural sources of enzyme inhibitors to offset postprandial hyperglycemia. The objective of this study was to evaluate coastal Alaskan seaweed inhibition of α-glucosidase and α-amylase, two carbolytic enzymes involved in serum glucose regulation. Of the six species initially screened, the brown seaweeds Fucus distichus and Alaria marginata possessed the strongest inhibitory effects. F. distichus fractions were potent mixed-mode inhibitors of α-glucosidase and α-amylase, with IC50 values of 0.89 and 13.9 μg/mL, respectively; significantly more efficacious than the pharmaceutical acarbose (IC50 of 112.0 and 137.8 μg/mL, respectively). The activity of F. distichus fractions was associated with phlorotannin oligomers. Normal-phase liquid chromatography-mass spectrometry (NPLC-MS) was employed to characterize individual oligomers. Accurate masses and fragmentation patterns confirmed the presence of fucophloroethol structures with degrees of polymerization from 3 to 18 monomer units. These findings suggest that coastal Alaskan seaweeds are sources of α-glucosidase and α-amylase inhibitory phlorotannins, and thus have potential to limit the release of sugar from carbohydrates and thus alleviate postprandial hyperglycemia. 相似文献
977.
James F Grutsch Patricia A Wood Jovelyn Du-Quiton Justin L Reynolds Christopher G Lis Robert D Levin Mary Ann Daehler Digant Gupta Dinah Faith T Quiton William JM Hrushesky 《Journal of circadian rhythms》2011,9(1):1-12
Background
Many previous studies have documented seasonal variation in suicides globally. We re-assessed the seasonal variation of suicides in Finland and tried to relate it to the seasonal variation in daylength and ambient temperature and in the discrepancy between local time and solar time.Methods
The daily data of all suicides from 1969 to 2003 in Finland (N = 43,393) were available. The calendar year was divided into twelve periods according to the length of daylight and the routinely changing time difference between sun time and official time. The daily mean of suicide mortality was calculated for each of these periods and the 95% confidence intervals of the daily means were used to evaluate the statistical significance of the means. In addition, daily changes in sunshine hours and mean temperature were compared to the daily means of suicide mortality in two locations during these afore mentioned periods.Results
A significant peak of the daily mean value of suicide mortality occurred in Finland between May 15th and July 25th, a period that lies symmetrically around the solstice. Concerning the suicide mortality among men in the northern location (Oulu), the peak was postponed as compared with the southern location (Helsinki). The daily variation in temperature or in sunshine did not have significant association with suicide mortality in these two locations.Conclusions
The period with the longest length of the day associated with the increased suicide mortality. Furthermore, since the peak of suicide mortality seems to manifest later during the year in the north, some other physical or biological signals, besides the variation in daylight, may be involved. In order to have novel means for suicide prevention, the assessment of susceptibility to the circadian misalignment might help. 相似文献978.
Mary Jo Frazier Gale E. Kleinkopf Richard R. Brey Nora L. Olsen 《American Journal of Potato Research》2006,83(1):31-39
The use of industrial-type linear accelerators may be an alternative treatment for controlling sprouts in potatoes during long-term storage. The technical aspect of treatment has been described and may include largescale treatment of bulk potatoes as they are moving along a system of conveyors either to or from storage. The objective of the study was to evaluate the use of a linear accelerator for long-term sprout control under commercial storage conditions and the effects on tuber quality. An 18-MeV linear accelerator was used to provide sprout-inhibiting irradiation to various lots of ‘Russet Burbank’ potatoes. Successful sprout suppression was achieved with doses of 40 to 50 Gy (0 g sprout weight after 9 months of storage at 7.2 C) while higher doses caused undesirable increases in reducing sugars in the tubers. Glucose concentrations of treated tubers (0.25% fresh tuber weight) were higher after treatment than the untreated controls (0.08%), but returned to control levels after 2 to 6 months in storage. Glucose concentrations in treated tubers were significantly reduced with storage management for reconditioning by increasing the storage temperature for one month (from 7.2 C to 14.4 C) resulting in glucose concentrations near control concentrations. By increasing the dose-rate from the accelerator, recovery of tuber glucose concentration due to radiation treatments was significantly faster than when the dose was given more slowly. Potato tubers irradiated with high rates (100 Gy) of high-energy electrons exhibited an increase in soft rot and dry rot incidence in storage. Disease development in potatoes treated with sprout inhibiting doses of radiation (50 Gy) increased in lots with a high rot potential. Treated lots with low rot potential were successfully stored for 8 months or more without an increase in rot potential or shrinkage. Selection of lots for irradiation treatment should include some preliminary evaluation for development of disease in long-term storage. 相似文献
979.
Helen H. Cunningham Mary V. Zaehringer Walter C. Sparks 《American Journal of Potato Research》1971,48(9):320-328
Idaho-grown Russet Burbank potatoes treated with a sprout inhibitor were stored at 42 F, 45 F, and 48 F for 11 months. Monthly quality evaluations showed that tubers stored at 42 F were less mealy, contained more reducing sugars, and were yellower in color than those stored at 45 F or 48 F. The latter two treatments were not significantly different in mealiness and were generally similar in yellowness of color. Tubers stored at 48 F had less reducing sugars than those stored at 45 F. Although all treatments resulted in a gradual darkening of the potatoes during storage, differences in grayness among the three storage treatments were minor from a practical standpoint. It is suggested that a storage temperature no lower than 45 F be used to maintain the internal quality of Russet Burbank potatoes. 相似文献
980.