全文获取类型
收费全文 | 1112篇 |
免费 | 87篇 |
国内免费 | 1篇 |
专业分类
林业 | 53篇 |
农学 | 40篇 |
基础科学 | 4篇 |
243篇 | |
综合类 | 62篇 |
农作物 | 51篇 |
水产渔业 | 127篇 |
畜牧兽医 | 530篇 |
园艺 | 22篇 |
植物保护 | 68篇 |
出版年
2024年 | 3篇 |
2023年 | 17篇 |
2022年 | 15篇 |
2021年 | 39篇 |
2020年 | 34篇 |
2019年 | 30篇 |
2018年 | 40篇 |
2017年 | 36篇 |
2016年 | 39篇 |
2015年 | 32篇 |
2014年 | 63篇 |
2013年 | 82篇 |
2012年 | 79篇 |
2011年 | 125篇 |
2010年 | 47篇 |
2009年 | 49篇 |
2008年 | 82篇 |
2007年 | 50篇 |
2006年 | 45篇 |
2005年 | 55篇 |
2004年 | 39篇 |
2003年 | 35篇 |
2002年 | 32篇 |
2001年 | 11篇 |
2000年 | 13篇 |
1999年 | 16篇 |
1998年 | 8篇 |
1997年 | 7篇 |
1995年 | 5篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 5篇 |
1991年 | 6篇 |
1990年 | 5篇 |
1989年 | 8篇 |
1988年 | 7篇 |
1987年 | 4篇 |
1986年 | 6篇 |
1984年 | 4篇 |
1983年 | 5篇 |
1982年 | 1篇 |
1980年 | 2篇 |
1978年 | 3篇 |
1976年 | 2篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1972年 | 1篇 |
1970年 | 1篇 |
1969年 | 1篇 |
1968年 | 2篇 |
排序方式: 共有1200条查询结果,搜索用时 31 毫秒
21.
F. Martinez Gómez S. Hernández Rodriguez I. Navarrete López-Cózar R. Calero Carretero 《Veterinary parasitology》1980,7(1):33-38
A serological study of intermediate hosts (cattle, sheep, goats and swine) of Echinococcus granulosus was carried out by means of the indirect haemaglutination (IHA) and flocculation with latex (LA) tests, relating them with the organs invaded, and the fertility and viability of the cysts.In cattle a sensitivity of 75% and 21.05% of non-specific reactions was given by IHA, while the LA gave 66.7 and 17.54%, respectively. In the sheep, the IHA gave 79.16 and 20%, and the LA, 75 and 15%. In the goat, the IHA gave 76 and 22%, and the LA 71.43 and 16%. Finally, in the pig, the IHA gave 75 and 22%, and the LA, 66.6 and 14.16%.The IHA was more sensitive in detecting hepatic cysts in the cow, and pulmonary cysts in the goat, while in the sheep and the pig there were no significant differences. The LA was more sensitive in detecting hepatic cysts in the cow, goat and pig, while there were no significant differences in sheep.The differences in serum titers by the IHA and the LA tests with respect to the viability and the fertility of the cysts in the hosts, were not significant. 相似文献
22.
23.
Martínez-Larrañaga MR Anadón A Fernandez-Cruz ML Díaz MJ Martinez MA Frejo MT Martínez M Tafur M 《Journal of veterinary pharmacology and therapeutics》2000,23(1):37-44
The potential cytotoxic effects of the compounds 8-quinolinol, chloramine-T and natamycin have been studied in isolated pig hepatocytes. The relative cytotoxicity of these compounds was evaluated on the basis of the leakage of cytosolic lactate dehydrogenase (LDH), 3-(4,5 dimethyl)thiazol-2-yl,-2,5-diphenyl tetrazolium bromide (MTT) reduction by mitochondrial dehydrogenases, uptake of neutral red (NR) by cytosolic lysosomes, glutathion (GSH) depletion and oxidized glutathion (GSSG) efflux after 24 h exposure. Evaluation of the 20%, 50% and 80% reduced absorbance data obtained from the parameters NR20, NR50, and NR80, and MTT20, MTT50 and MTT80 enabled us to rank these compounds in decreasing order of cytotoxicity: 8-quinolinol > natamycin > chloramine-T. Also for the parameters LDH and GSH, chloramine-T appears to be less cytotoxic than natamycin and 8-quinolinol. Our study demonstrated that pig hepatocytes may be a useful model for examining cytotoxic events of drugs to be used in pigs, therefore avoiding possible extrapolation problems due to species differences. 相似文献
24.
The induction of endometrial prostaglandin (PG) F2 synthesis by oxytocin is dependent upon activation of phospholipase (PL) A2 and mobilization of arachidonic acid. The objective of this study was to determine if oxytocin stimulates PGF2 synthesis by inducing synthesis of cytosolic PLA2 (cPLA2). In Experiment 1, 15 ovariectomized ewes were given progesterone and estradiol to simulate an estrous cycle. Ewes were then given an injection of oxytocin on Day 14 of the simulated estrous cycle. Jugular blood samples were collected and assayed for 13,14-dihydro-15-keto-prostaglandin F2 (PGFM). Uteri were collected at 0, 7.5, 25, 90, or 240 min postinjection (n = 3 ewes/time point). Total RNA was isolated from caruncular endometrium and subjected to dot-blot analysis. Oxytocin induced a rapid and transient increase in serum PGFM (P < 0.01). However, endometrial concentrations of cPLA2 mRNA did not change following oxytocin administration (P > 0.10). In Experiment 2, 11 ovary-intact ewes were given oxytocin (n = 5) or saline (n = 6) on Day 15 after estrus. Jugular blood samples were collected and assayed for serum concentrations of PGFM. Uteri were collected at 15 min postinjection. Homogenates were prepared from caruncular endometrium and subjected to Western blot analysis. Concentrations of PGFM were higher in oxytocin treated ewes compared to saline treated ewes at 15 min postinjection (P < 0.01). Endometrial concentrations of cPLA2 protein were greater in the cytosolic than in the microsomal fraction (P < 0.01). Oxytocin did not affect the amount of cPLA2 protein in either fraction (P > 0.10). In conclusion, oxytocin did not effect expression of either cPLA2 mRNA or protein in ovine endometrium. Oxytocin may stimulate PGF2 synthesis by activating cPLA2 protein that is already present in an inactive form. 相似文献
25.
García ME Caballero J Toni P Garcia I Martinez de Merlo E Rollan E Gonzalez M Blanco JL 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2000,47(4):243-249
We describe a case of canine mycoses initially diagnosed by clinical signs and enzyme-linked immunosorbent assay anti-fungal test, and later confirmed by the isolation of Paecilomyces sp. during the post-mortem examination. The fungus was isolated from lesions in the kidneys, mitral valve, abdominal aorta and vertebral discs. In this kind of process, it is important to identify the responsible agent early in order to make a study of anti-fungal susceptibility and establish effective treatment. 相似文献
26.
FJ Pena JA Gines J Duque V Vieitez R Martinez –Pérez L Madejón I Nuñez Martinez JM Moran S Fernández-García 《Reproduction in domestic animals》2006,41(2):189-190
A 6-year-old female Alaska Malamute dog was presented for evaluation of abdominal enlargement referred by a local veterinarian. On the history, the owner complained of chronic abdominal enlargement initiated more than 4 months ago, reduced appetite, occasional vomiting and general dullness. He also complained of greenish mucous intermittent vaginal discharge starting 10 days ago. The bitch was chronically treated with medroxiprogesterone acetate. A laparatomy was performed and fluid in the abdomen was found and aspirated during the surgery. Also a very fluid-filled distended uterus and a mass in the distal part of the left uterine horn were found. The mass was encapsulated by the omentum, but areas of necrosis and calcification were identified. Histopathological diagnosis was endometrial adenocarcinoma. 相似文献
27.
Hygienic and ecological risks connected with utilization of animal manures and biosolids in agriculture 总被引:2,自引:0,他引:2
In recent years the fate of human and animal pathogen microorganisms as a potential pollutant of the environment has been paid increased attention. Substantial quantities of these compounds and their metabolites are excreted, flushed down the drain, discarded as waste, or left over in animal feedlots. After passing to the sewer, several of these compounds are not adequately eliminated by the methods that are currently used in sewage treatment. Substantial quantities of biosolids and livestock manure end up on agricultural land. Effective sanitation of the environment, particularly of some of its special parts, which can be a source of spreading of diseases, plays an important role in prevention of infectious diseases. In this respect special attention should be paid to the disinfection of infected farm animal excrements. Sanitation of excrements should, on the one hand, ensure effective inhibition of infectious agents and, on the other hand, comply with the requirement of preserving the composition of the manure so it can be used in agricultural production. 相似文献
28.
29.
Noelia Ibez María Antonía Santacreu Maria Martinez Agueda Climent Agustín Blasco 《Livestock Science》2006,101(1-3):126-133
An experiment of selection for ovulation rate was carried out. Animals were derived from a synthetic line first selected 12 generations for litter size, then 10 generations for uterine capacity. Selection was relaxed for 6 generations. Selection was based on the phenotypic value of ovulation rate with a selection pressure on does of 30%. Males were selected from litters of does with the highest ovulation rate. Males were selected within sire families in order to reduce inbreeding. Ovulation rate was measured in the second gestation by a laparoscopy, 12 days after mating. Each generation had about 80 females and 20 males. Results of three generations of selection were analyzed using Bayesian methods. Marginal posterior distributions of all unknowns were estimated by Gibbs sampling. Heritabilities of ovulation rate (OR), number of implanted embryos (IE), litter size (LS), embryo survival (ES), fetal survival (FS), and prenatal survival (PS) were 0.44, 0.32, 0.11, 0.26, 0.35, and 0.14, respectively. Genetic correlation between OR and LS was 0.56, indicating that selection for ovulation rate can augment litter size. Response to selection for OR was 1.80 ova. Correlated responses in IE and LS were 1.44 and 0.49, respectively. Selection for ovulation rate may be an alternative to improve litter size. 相似文献
30.
In order to offer hints for new research, the agronomic techniques used to manageOrobanche crenata in Sicily in the late 19
th
and early 20
th
Century are examined; the publications in which they were originally described can be obtained only with difficulty. Hand
pulling, resistant cultivars and late sowing are the techniques used nowadays. Screening for resistance to broomrape of the
traditional cultivars of broad bean, and for the influence of sowing density on broomrape infestations, is still to be done. 相似文献