Outbreak investigations and genetic characterization of foot-and-mouth disease virus in Ethiopia in 2008/2009

The study was conducted in three regional states of Ethiopia: Amhara, Oromia, and Addis Ababa from August 2008 to April 2009 with the objectives of identifying the genetic diversity of serotypes and topotypes in Ethiopia, and determining the attack rate and associations of potential risk factors with foot-and-mouth disease (FMD) seropositivity. A total of 496 cattle were clinically and serologically examined for presence of specific lesions and nonstructural protein for FMD, respectively. Of which, 140 (28.2%) manifested clinical signs and lesions suggestive of FMD, and 219 (44.2%) were seropositive. From a total of 7,781 animals observed and recorded on a designed format in six districts, 1,409 (19.6%) were infected, and 15 (0.12%) died during outbreaks of FMD. Epidemiological investigations revealed that the morbidity rate of the disease was 21.1% in Akaki-kality sub-city, but the mortality rate was <2% in all districts. Furthermore, the mortality and case fatality rates were relatively higher, 1.6% and 8.9% in calves than the other age groups, respectively. From a total of 33 bovine epithelial tissue-cultured samples, 19 (57.6%) showed CPE for FMD virus, in which 16 samples had serotype O and EA-3 topotype, while three samples had found serotype A, Africa topotype, and G-VII strain. Various strains of FMD viruses were isolated in Ethiopia in this study, and therefore, further detailed studies on the evaluation of available vaccines and the development of a vaccine which contains cocktails of antigens of FMD virus strains in the country should be encouraged.     

CRETINISM IN A NORTH AMERICAN BLACK BEAR(URSUS AMERICANUS)

Congenital hyperplastic goiter and cretinism were documented in a 16 month-old male North American black bear (Ursus americanus). The cub was captured at approximately 8 months of age and maintained for an additional 8 months in captivity. Clinical signs included growth retardation, clumsiness, and facial dysmorphism. Hypothyroidism was documented by determining serum triiodothyronine (T3) and thyroxine (T4) levels. Lysosomal storage disease was ruled out by measuring various lysosomal enzyme activities. Serologic, radiographic, computed tomographic, necropsy, and histopathologic findings were consistent with congenital hypothyroidism and cretinism.     

Measurement of vertical forces and temporal components of the strides of horses using instrumented shoes

Instrumented shoes were used to measure the vertical forces exerted by horses moving at a variety of gaits. Two types of shoes were used; one contained a single transducer positioned over the center of the frog and the second contained transducers located at the toe and both sides of the heel. Horses were shod with these instrumented shoes and walked and trotted over a force plate. Forces were simultaneously recorded from the transducers in the shoes and from the force plate. Comparisons were made between the amount and duration of the forces exerted on the transducers and the vertical and horizontal forces recorded from the force plate. Forces recorded from the single transducer shoes showed strong correlations with the forces recorded from the force plate for horses moving at the walk; however, at the trot only moderate correlations occurred between these forces. At both the walk and trot, forces recorded from each side of the heel and the total forces occurring on all three transducers from the front hooves of horses shod with three transducer shoes showed strong correlations to the vertical forces recorded from the force plate.Vertical forces were also recorded from the instrumented shoes as horses walked, trotted and galloped on a track straightaway. Forces recorded from normal horses shod with single transducer shoes on all four feet were greater on the forelimbs than the rear limbs at the walk and trot. At the gallop, forces were highest on the lead front followed by the nonlead front, lead rear and nonlead rearlimb, respectively. Forces recorded from a three transducer shoe on the right front hoof of a horse walking, trotting and galloping in a right lead were highest on the medial side of the heel and occurred during the middle of the support period. Peak forces on the toe occurred at or near the time of heel lift.The results of these studies indicate that these instrumented shoes have advantages over the methods previously used to measure locomotor forces. These instrumented shoes can be used to simultaneously record the temporal components and the amount and distribution of vertical forces exerted during consecutive strides of horses moving at a variety of gaits.     

Biomonitoring and Sourcing Toxic Elements Using Vascular Epiphytes of the Tillandsia Genus in the Mining Region of Taxco de Alarcón,Guerrero, Southern Mexico

Water, Air, &; Soil Pollution - The concentration of As, Pb, Cd, Zn, Fe, Mn, and Cu and the Sr and Pb isotopic compositions were determined in four species of the vascular epiphyte Tillandsia...     

Increase in phosphorylase activity during cold-induced sugar accumulation in potato tubers

Summary The accumulation of reducing sugars, sucrose and hexose phosphates in cv. Bintje and genotype KW77-2916 during storage at 2, 4, or 8°C was studied in relation to several catalytic activities. Bintje tubers accumulated sugars during storage at 2 or 4°C, whereas KW77-2916 showed reduced cold-sweetening at 2°C. The increase in glucose 6-phosphate and sucrose occurred concurrently and preceded the increase in reducing sugar concentration. Phosphorylase activity showed a strong interaction with temperature, storage duration and sugar accumulation in both genotypes. Invertase activity increased in Bintje concomitantly with the increase in reducing sugars, but this effect was less obvious in KW77-2916. The activities of other glycolytic and Krebs cycle enzymes showed no obvious correlation with sugar accumulation. It is suggested that the increase in phosphorylase activity acts as a triggering event in the sweetening of potato tubers during cold storage.     

Determination of ephedrine alkaloids and synephrine in dietary supplements by column-switching cation exchange high-performance liquid chromatography with scanning-wavelength ultraviolet and fluorescence detection

An HPLC method with on-line cleanup coupled to the separation column is described for determination of (-)-norephedrine, (+)-norpseudoephedrine, (-)-ephedrine, (+)-pseudoephedrine, (-)-N-methylephedrine, (+)-N-methylpseudoephedrine, and (+/-)-synephrine in finished dietary supplement products. Test portions were extracted in acidified aqueous acetone. A filtered aliquot was cleaned up on a strong cation exchange (SCX) precolumn that later was automatically coupled to the SCX analytical column. Measurement was by full-scan UV spectra for confirmation of identity by spectral matching and real-time integration of three wavelength signals for multiple quantitation. (+/-)-Synephrine was also quantitated by native fluorescence. Recovery averaged 95-100%. Determination of the major ingredients (-)-ephedrine, (+)-pseudoephedrine, and (+/-)-synephrine compared favorably to findings by an independent LC-MS analysis for a set of 25 samples. The results of a survey were reported for total ephedrine alkaloid and synephrine content and were compared to content declaration, for approximately 48 finished products.     

Oomycete species associated with Theobroma cacao crops in Colombia

The study of oomycetes associated with crops is highly important due to the economic losses they might cause. In cacao, the genus Phytophthora has been extensively studied, but little is known about other genera and species of oomycetes associated with this plant. This study aimed to determine the oomycetes’ diversity present in Colombian cacao crops. A total of 146 isolates were obtained from diseased plants and soil in 11 departments. Analysis of internal transcribed spacer (ITS) and cytochrome oxidase subunit I (coxI) sequences was performed along with the assessment of morphological characteristics. Nine species were identified, distributed in four genera: Phytophthora (P. palmivora, 54%; P. nicotianae, 1%), Phytopythium (Phy. chamaehyphon, 15%; Phy. cucurbitacearum, 9%; Phy. vexans, 7%; Phy. helicoides, 1%), Globisporangium (G. splendens, 3%), and Pythium (Py. delicense, 1%; Py. inflatum,1%). Additionally, an unidentified and possibly new species of Phytophthora (5%) and three unidentified species of Phytopythium (3%) were found. This is the first report of Globisporangium, Phytopythium, and Pythium in cacao crops of Colombia and the first report of the species Phy. chamaehyphon in the country. Interestingly, some isolates of Phytopythium spp. were isolated from necrotic leaves and vascular section of stems, which may suggest a role in cacao diseases traditionally associated with Phytophthora. Also, it is proposed that the new species of Phytophthora may be contributing significantly to black pod disease in Colombian cacao crops, and we highlight that the study of P. palmivora is urgent because of its distribution all over the country.     

Effect of annealing from traditional nixtamalisation process on the microstructural,thermal, and rheological properties of starch and quality of pozole

Eleven maize landraces were evaluated for pozole quality. The microstructural, thermal and rheological properties of annealed starch granules determine most of the quality of pozole. Annealed starch in traditional nixtamalisation has an important role in increasing gelatinisation onset (To), peak (Tp) and final (Tf) temperatures; peak, setback and final viscosity as well as the stability of the starch granule, all of which significantly affect pozole quality. Annealed starch in Cacahuacintle nixtamal (pozole end-use) increased temperatures To, Tp and Tf by >5.2, >3.8 and >4.1 °C respectively, and narrowed the range Tf − To from 13.78 to 12.62 °C. The enthalpy was reduced from 6.76 to 5.85 J/g, while the nixtamal starch in tortilla maize landraces presented fewer annealing effects. The annealing effect in nixtamal starch seems to stabilize the starch granules and avoid their collapse, compared to native starch, as shown by the X-ray diffraction peak intensity and pattern that is similar to unprocessed maize. Starch in nixtamal changes from Type A to Type V pattern in pozole. Kernel physical parameters, although important, affected the quality to a lesser extent, with the exception of the flotation index. Cacahuacintle maize landrace showed the best quality and yield as well as a short pozole cooking time.     

Soil Physicochemical Changes Following Buffelgrass Establishment in Mexico

Clearing brush from rangeland and seeding it to buffelgrass (Cenchrus ciliaris L.) is a popular range improvement practice in Mexico, but no data are available on the effects of these practices on soil properties. Twenty-nine study sites were randomly selected across 3 major climatic regions in Mexico: 13 in the northwest, 11 in the northeast, and 5 in the southeast. Soils under buffelgrass stands more than 10 years old and on adjacent virgin rangelands were sampled at 0-10, 10-20, and 20-30 cm depths to quantify sand, silt, clay, organic C, total N, pH, EC, CEC, available P, and exchangeable Ca2+, Mg2+, Na+, and K+.Because soil variables were not significantly (P 0.05) different among depths, only data from the surface horizon received further analysis. Soils in the northwest had higher (P 0.05) sand content and were particularly low in total N (0.08%), Ca2+, and K+, while soils of wetter and warmer southeast Mexico were significantly (P 0.05) higher than the other two regions in measures of soil fertility such as organic C (10 times higher), total N, and CEC (both at least 3 times higher). Long-term buffelgrass establishment had the most effect on soils in the southeast and no significant effect (P0.1) on soils of northeast Mexico. Soil organic C and total N both declined (P 0.025) by about 40% under buffelgrass pastures in the southeast; excgangeable Ca2+ decreased (P=0.027) by 21% in the same region, and Mg2+ declined (P=0.03) by 36% under buffelgrass in the northwest. Soil P did not respond to buffelgrass establishment. The findings of this study suggest that high forage yields following conversion of rangeland to buffelgrass pasture will be less sustainable in subtropical regions with high rainfall (mean of 1070 mm in this study) than in the semiarid zone with inherently poorer soils.