Effects of dietary supplementation with probiotic live yeast Debaryomyces hansenii on the immune and antioxidant systems of leopard grouper Mycteroperca rosacea infected with Aeromonas hydrophila

Two trials were conducted to determine the efficacy of fish fed live yeast Debaryomyces hansenii strain CBS 8339 on immune and antioxidant systems in leopard grouper Mycteroperca rosacea infected with Aeromonas hydrophila. Juveniles (12±0.5 g) were fed with a control diet or a D. hansenii‐supplemented diet (10⁶ colony‐forming units per gram) for 5 weeks. The live weight of fish was registered on a weekly basis. After 4 weeks, fish from each treatment were immunocompromised with pathogenic A. hydrophila and further fed for 1 week in order to evaluate the effect on immunological and antioxidant parameters. Generally, the results showed enhanced growth performance in fish fed the diet containing yeast compared with the control. Addition of live yeast had no significant effect on the immunological parameters after 4 weeks of feeding. However, post infection with A. hydrophila fish fed the yeast‐supplemented diet resulted in a significant increase in the levels of plasmatic immunoglobulin M. Superoxide dismutase and catalase (CAT) activities were significantly higher in the yeast group. In this fish, CAT and heat shock protein 70 genes were up‐regulated before and after infection of A. hydrophila. The present study is the first one reporting that yeast (D. hansenii) can enhance immunity and resistance against A. hydrophila.     

Diverse migratory strategies for a hawksbill sea turtle population

相似文献   

Protein lipid ratio for the growth and survival of juvenile crayfish Procambarus acanthophorus (Hobbs 1972) reared under controlled conditions

The influence of dietary protein/lipid levels on growth and survival in juvenile crayfish (Procambarus acanthophorus) was evaluated during a 12‐week nutritional trial. Twenty experimental diets were formulated containing different protein (200, 250, 300, 350 and 400 g kg^?1) and lipid (60, 80, 100 and 120 g kg^?1) levels. A bifactorial model (5 × 4) was used with three replicates and 15 crayfish (0.014±0.006 g) per replicate, in a recirculation system with 60–15 L rectangular tanks and a biological filter, the culture conditions were a photoperiod of 12:12 h L:D and temperature 24.7±1.0 °C. Total survival ranged from 66.6% to 86.6%, with no differences attributed to treatments. Dietary protein levels had a significant effect on growth gain and nutritional efficiency; in contrast, lipid levels had no effect on juvenile performance. The 250/120 (protein/lipid) treatment promoted the most efficient growth (final weight: 3.09 g, weight gain: 21 995%, specific growth rate: 3.35% day^?1); the less efficient growth was observed in juveniles fed with 400 g kg^?1 protein diets. The quadratic regression model indicated 279 g kg^?1 as the optimal protein level for crayfish growth, while levels between 211 and 232 g kg^?1 could minimize the feeding costs without affecting growth.     

Reuse of water from biofloc technology for intensive culture of Nile tilapia ( Oreochromis niloticus ): effects on productive performance,organosomatic indices and body composition

During a growout period of 14 weeks, the productive performance, organosomatic indices and body composition of the Nile tilapia Oreochromis niloticus cultivated at high density reusing the water from systems with biofloc technology (BFT) were evaluated. Two treatments: tilapia cultured in biofloc (TB) and tilapia cultured in reused water biofloc (RW) were established. Mixed sex, juvenile Nile tilapia (average weight and length: 79.28 ± 14.44 g and 12.44 ± 0.70 cm) were stocked into 6 experimental units (0.2 m3) at a density of 100 fish/m3. Temperature, dissolved oxygen, total dissolved salts, pH, NH4–N, NH3–N and NO3–N were recorded daily, while NO2–N, general hardness, carbonate hardness and settleable solids were recorded weekly. The weight and length of the tilapias were recorded biweekly. Survival, productive performance, proximal composition and organosomatic indices of Nile tilapia were evaluated. The water parameters in RW treatment such as pH, total dissolved salts, NO2–N, NO3–N and carbonate hardness were significantly greater (p < 0.05), when compared to those obtained in TB treatment. Survival rate was > 98% under both treatments. The final weight (TB = 163.09 ± 42.34 g, RW = 159.23 ± 39.92 g), protein (19.1%) and lipid (2.2%) content of the tilapia were non-significantly different between treatments. In addition, no significant differences in the gonadosomatic and hepatosomatic indices were observed either between tilapia sex or between the treatments. The results suggest that the intensive cultivation of Nile tilapia in biofloc can be established using reuse water from BFT systems, without adverse effects on their survival, productive performance, proximal composition and gonadal development.     

In Vitro and In Vivo Feedstuff Digestibility for Snook,Centropomus undecimalis,Juveniles

The degree of hydrolysis (DH) of the ingredients was evaluated for Centropomus undecimalis juveniles. The in vitro experiment included wheat gluten (WG), corn gluten (CG), soybean meal (SBM) and pretreated phytase (SBM + phytase), soy protein concentrate (SPC), canola meal (CAN) and pretreated phytase (CAN + phytase), poultry byproduct meal (PBPM), chicken meal (CHKM), dried whey (DW), Pota meal (PM: mix of giant squid, fish soluble protein concentrate, crustacean meal, and fish oil), and Protiblend (PTB: aquatic and render mix). The highest acidic DH occurred with PTB (0.38 ± 0.06), CHKM (0.33 ± 0.3), and PBPM (0.25 ± 0.03). In the alkaline condition, PTB (1.6 ± 0.17 and 0.98 ± 0.05 for pyloric caeca and intestine, respectively) and CG (1.04 ± 0.4 and 0.75 ± 0.2 for caeca and intestine, respectively) provided the highest DH values. In vitro digestibility demonstrated that PTB was the highest (85.3%) followed by PBPM (51.4%), CAN + phytase (47.6%), CG (45.1%), and CHKM (46.5%). The in vivo experiment concerned the WG, CHKM, PBPM, PM, and PTB diets plus a reference diet with fishmeal (Ref diet). The total %DH was different (P < 0.05) with the lowest values for the WG diet (0.34 ± 0.09%) and Ref diet (0.34 ± 0.15). Free amino acid released during digestion was displayed for these diets and a bifactorial analysis produced no difference (P > 0.05). The apparent digestive coefficients ranged from 89.8 to 92.9% for protein and from 68 to 71.4% for energy.     

Fish biodiversity sampling in stream ecosystems: a process for evaluating the appropriate types and amount of gear

相似文献