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101.
Asensio L González I Fernández A Rodríguez MA Hernández PE García T Martín R 《Journal of agricultural and food chemistry》2001,49(4):1720-1723
A method of DNA analysis has been developed to verify the authenticity of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets. A short fragment (208 bp) of the mitochondrial 12S rRNA gene was amplified by the polymerase chain reaction and analyzed by single-strand conformation polymorphism to get species-specific patterns of single-stranded DNA (ssDNA). DNA strands were separated by native polyacrylamide gel electrophoresis and visualized by silver staining. Discrimination among the three fish species studied was possible, because each one expressed a specific ssDNA pattern. 相似文献
102.
103.
C.M. Morvillo E.B. de la Fuente A. Gil M.A. Martínez-Ghersa J.L. González-Andújar 《European Journal of Agronomy》2011,34(4):211-221
Annual wormwood interference on soybean crop growth and yield may result from competition and allelopathy, which are modulated by crop management. Allelochemicals released by annual wormwood (e.g. artemisinin) may affect the crop directly or indirectly through the effect on the nitrogen fixing symbiont, Bradyrhizobium japonicum. The objectives were (i) to quantify the crop response (i.e. biomass production, nodulation and yield) to weed interference and (ii) to determinate the relative change of competition and allelopathy interferences, when a sublethal dose of herbicide is applied. Two split plot field experiments with three replications were used. The experiment involved a factorial combination of five weed–crop density (soybean/annual wormwood, plants m?2) levels: D1, pure soybean, 40/0 plants m?2; D2, 40/2 plants m?2; D3, 40/4 plants m?2 and D4, 40/8 plants m?2, and D5, pure annual wormwood, 0/8 plants m?2, two activated carbon (allelopathy) levels: C?, with activated carbon (reduced allelopathy) and C+, non activated carbon applied (with allelopathy) and two herbicide levels: H?, untreated and H+, treated with a sub-lethal dose of glyphosate. Activated carbon to adsorb allelochemicals (with and without activated carbon) and glyphosate application (with and no herbicide) were assigned to sub-plots. Increasing weed density did not affect crop biomass at flowering, but changed nodule number and soybean yield with a different pattern depending on carbon and herbicide treatment. Relative crop yield decreased with increasing relative weed biomass. This decrease was particularly drastic when allelopathy was reduced by activated carbon and without herbicide application. The maximum yield losses of 33% in 2006 and 17% in 2007 were observed with the highest weed density (8 plants m?2). In contrast, without carbon (high allelopathy level), soybean yield remained stable within the explored range of annual wormwood biomass, despite the fact that weed biomass at high densities (D4) was high enough to generate competition. The lack of response to increasing weed density could be related to the indirect effect of allelochemicals interacting with soil microorganisms (i.e. B. japonicum) that positively affected the nodulation (e.g. larger nodules in 2006 and increased nodules biomass due to higher number of roots in 2007 at high densities). With herbicide application, soybean yield of both carbon treatments remained stable when biomass of annual wormwood increased. This research provided strong evidence in support of the existence of positive effect of allelopathic and competitive interactions between annual wormwood and soybean crop under field conditions that may be overridden under herbicide application. 相似文献
104.
The Penman–Monteith equation for reference evapotranspiration (ETo) estimation cannot be applied in many situations, because climatic records are totally or partially not available or reliable.
In these cases, empirical equations that rely on few climatic variables are necessary. Nevertheless, the uncertainty associated
with empirical model estimations is often high. Thus, the improvement of methods relying on few climatic inputs as well as
the development of emergency estimation tools that demand no local climatic records turns into a task of great relevance.
The present study describes different approaches based on multiple linear regression, simple regression and artificial neural
networks (ANNs) to deal with ETo estimation exclusively from exogenous records from secondary stations. This cross-station approach is based on a continental
characterization of the study region, which enables the selection and hierarchization of the most suitable ancillary data
supplier stations. This procedure is compared with different traditional and cross-station approaches, including methodologies
that also consider local temperature inputs. The proposed methods are also evaluated as gap infilling procedures and compared
with a simple methodology, the window averaging. The artificial neural network and the multiple linear regression approaches
present very similar performance accuracies, considerably higher than simple regression and traditional temperature-based
approaches. The proposed input combinations allow similar performance accuracies as ANN models relying on exogenous ETo records and local temperature measurements. The cross-station multiple linear regression procedure is recommended due to
its higher simplicity. 相似文献
105.
106.
Marta Crivos María Rosa Martínez María Lelia Pochettino Carolina Remorini Cynthia Saenz Anahí Sy 《Agriculture and Human Values》2004,21(2-3):111-125
Through the ethnographic record of the subsistence activities partially or completely performed in the domestic sphere in two Mbyá-Guaraní settlements in Misiones (Argentina), we outline factors important in describing the local natural environment. Data was collected through systematic observation and also through semi-structured interviews. Analysis indicates that the natural environment of the area is characterized by the indigenous community in several different ways. Thus, local people view the environment as made up of different “micro-environments,” and they consequently think of the elements that compose these “micro-environments” as having different, distinct characteristics. In the context of their daily activities, both these “micro-environments” and the elements that compose them are regarded as resources, in that they are viewed in terms of what is in them relevant to the subsistence of these groups in the rainforest. It is the intention of this study to initiate the systematic recording and processing of information on how these indigenous communities know and manage the natural resources available to them in their daily life. 相似文献
107.
108.
In the experiments described the following observations have been made: (1) The toxic and diabetogenic action of alloxan increases in rats fed a low protein diet and even more so in the case of a high lard or ox fat diet. (2) The action of these fat diets was corrected by the addition of methionine, thiouracil, or coconut oil, but there was no modification either by addition of choline or sulfonamide or by increase in the protein proportion. (3) In rats fed other high fat diets (olive oil, butter) the actions of alloxan were not modified, but there was a slight diminution when high oleomargarine or corn oil diets were fed. However, complete protection was observed when a high coconut oil diet was administered. (4) The unfavorable effect of the high lard diet was observed also on the initiation and evolution of diabetes due to subtotal pancreatectomy. Contrarily, feeding a high protein diet and especially treatment with thiouracil had a favorable effect. (5) Diabetes appeared first in subtotal pancreatectomized rats which were overfed, then in those which ate ad libitum, and finally in those which were underfed. Diabetes appeared in rats fed a single meal before it did in those eating the same amount of food divided into three meals. 相似文献
109.
Muñoz J Felicísimo AM Cabezas F Burgaz AR Martínez I 《Science (New York, N.Y.)》2004,304(5674):1144-1147
Anisotropic (direction-dependent) long-distance dispersal (LDD) by wind has been invoked to explain the strong floristic affinities shared among landmasses in the Southern Hemisphere. Its contribution has not yet been systematically tested because of the previous lack of global data on winds. We used global winds coverage from the National Aeronautics and Space Administration SeaWinds scatterometer to test whether floristic similarities of Southern Hemisphere moss, liverwort, lichen, and pteridophyte floras conform better with (i) the anisotropic LDD hypothesis, which predicts that connection by "wind highways" increases floristic similarities, or (ii) a direction-independent LDD hypothesis, which predicts that floristic similarities among sites increase with geographic proximity. We found a stronger correlation of floristic similarities with wind connectivity than with geographic proximities, which supports the idea that wind is a dispersal vehicle for many organisms in the Southern Hemisphere. 相似文献
110.
McEwen AS Hansen CJ Delamere WA Eliason EM Herkenhoff KE Keszthelyi L Gulick VC Kirk RL Mellon MT Grant JA Thomas N Weitz CM Squyres SW Bridges NT Murchie SL Seelos F Seelos K Okubo CH Milazzo MP Tornabene LL Jaeger WL Byrne S Russell PS Griffes JL Martínez-Alonso S Davatzes A Chuang FC Thomson BJ Fishbaugh KE Dundas CM Kolb KJ Banks ME Wray JJ 《Science (New York, N.Y.)》2007,317(5845):1706-1709
Water has supposedly marked the surface of Mars and produced characteristic landforms. To understand the history of water on Mars, we take a close look at key locations with the High-Resolution Imaging Science Experiment on board the Mars Reconnaissance Orbiter, reaching fine spatial scales of 25 to 32 centimeters per pixel. Boulders ranging up to approximately 2 meters in diameter are ubiquitous in the middle to high latitudes, which include deposits previously interpreted as finegrained ocean sediments or dusty snow. Bright gully deposits identify six locations with very recent activity, but these lie on steep (20 degrees to 35 degrees) slopes where dry mass wasting could occur. Thus, we cannot confirm the reality of ancient oceans or water in active gullies but do see evidence of fluvial modification of geologically recent mid-latitude gullies and equatorial impact craters. 相似文献