Acute BVDV-2 infection in beef calves delays humoral responses to a non-infectious antigen challenge

Immunosuppressive effects of an intranasal challenge with non-cytopathic bovine viral diarrhea virus (BVDV) 2a (strain 1373) were assessed through acquired and innate immune system responses to ovalbumin (OVA). Concurrent BVDV infection was hypothesized to delay and reduce the humoral response to ovalbumin (administered on days 3 and 15 post-inoculation). Infected animals followed the expected clinical course. BVDV titers, and anti-BVDV antibodies confirmed the course of infection and were not affected by the administration of OVA. Both the T-helper (CD4⁺) and B-cell (CD20⁺) compartments were significantly (P < 0.05) reduced in infected animals, while the gamma-delta T-cell population (Workshop cluster 1+, WC1⁺) decreased slightly in numbers. Infection with BVDV delayed the increase in OVA IgG by approximately 3 d from day 12 through day 21 post-inoculation. Between days 25 and 37 post-inoculation following BVDV infection the IgM concentration in the BVDV− group decreased while the OVA IgM titer still was rising in the BVDV+ animals. Thus, active BVDV infection delays IgM and IgG responses to a novel, non-infectious antigen.     

A minirhizotron imaging system to identify roots expressing the green fluorescent protein

The limited flexibility available in the configuration of commercial minirhizotron imaging systems makes it difficult to adapt these systems to new applications. It is also too expensive to introduce modifications, which are often very temporary to these systems at the end of the development process.In order to identify the roots of a single species in mixed plant stands, we developed a new minirhizotron imaging system that makes it possible to observe roots expressing green fluorescent protein (GFP). This system is based on affordable and easily obtainable components such as webcams. Here, we report a protocol to identify suitable webcams for constructing a minirhizotron imaging system and demonstrate the application of this protocol to build a minirhizotron imaging system that can identify the roots of a transformed maize plant expressing GFP.     

Spatial and temporal variability of soil water in drylands: plant water potential as a diagnostic tool

Arid and semi-arid regions are characterized by low rainfall and high potential evaporative demand. Here, water is the major limiting factor for plant growth and productivity. Soil and surface hydrology properties （e.g. field capacity, infikration rates） effectively control the water re-distribution in the ecosystem, a fact that is aggravated in arid environments. Information of the spatial and temporal accessibility of soil water in desert ecosystems is limited. The purpose of the studies is the application of plant water potential to estimate the spatial and temporal variations of soil water availability in different arid ecosystems of the Negev （Israel） and southern Morocco. As model plants the evergreen shrubs Retama raetam, Thymelaea hirsuta and trees （Acacia tortilis） were chosen. Seasonal and spatial variations of the pre-dawn water potential （ψpd） were examined as diagnostic tool to determine water availability on the landscape level. The seasonal differences in the pre-dawn water potential were less pronounced on the dune compared to the interdune. This showed a better water availability on the dune slope. Also in the investigated wadis systems spatial differences of the water potential could be detected and related to the vegetation pattern.     

Molecular Mechanisms by Which a Fucus vesiculosus Extract Mediates Cell Cycle Inhibition and Cell Death in Pancreatic Cancer Cells

Pancreatic cancer is one of the most aggressive cancer entities, with an extremely poor 5-year survival rate. Therefore, novel therapeutic agents with specific modes of action are urgently needed. Marine organisms represent a promising source to identify new pharmacologically active substances. Secondary metabolites derived from marine algae are of particular interest. The present work describes cellular and molecular mechanisms induced by an HPLC-fractionated, hydrophilic extract derived from the Baltic brown seaweed Fucus vesiculosus (Fv1). Treatment with Fv1 resulted in a strong inhibition of viability in various pancreatic cancer cell lines. This extract inhibited the cell cycle of proliferating cells due to the up-regulation of cell cycle inhibitors, shown on the mRNA (microarray data) and protein level. As a result, cells were dying in a caspase-independent manner. Experiments with non-dividing cells showed that proliferation is a prerequisite for the effectiveness of Fv1. Importantly, Fv1 showed low cytotoxic activity against non-malignant resting T cells and terminally differentiated cells like erythrocytes. Interestingly, accelerated killing effects were observed in combination with inhibitors of autophagy. Our in vitro data suggest that Fv1 may represent a promising new agent that deserves further development towards clinical application.     

Digestibility of microbial and mussel meal for Arctic charr (Salvelinus alpinus) and Eurasian perch (Perca fluviatilis)

Apparent digestibility coefficients (ADCs) for four protein‐rich alternative feed ingredients, intact baker's yeast (Saccharomyces cerevisiae), extracted baker's yeast (S. cerevisiae), zygomycetes (Rhizopus oryzae) and blue mussel (Mytilus edulis), were determined for Arctic charr (Salvelinus alpinus) and Eurasian perch (Perca fluviatilis). Diets contained 30% of test ingredients, and ADCs were compared to a reference diet containing fish meal. For Arctic charr, ADCs for dry matter (DM, 71–99%), sum of amino acids (SAA, 84–99%) and gross energy (70–99%) were significantly lower for intact S. cerevisiae than for extracted S. cerevisiae, R. oryzae and M. edulis. The ADCs for the indispensable amino acids (IAA) in Arctic charr varied between 84% and 99%. Significant differences were found in ADCs for IAA between the test ingredients for Arctic charr, with higher values for extracted S. cerevisiae and M. edulis. The ADCs in Eurasian perch varied between 83% and 95% for DM, 89% and 98% for CP, 92% and 100% for SAA, 81% and 96% for gross energy. No significant differences were found for ADCs between the test ingredients in Eurasian perch, indicating a species effect on digestibility. Furthermore, the absence of intact cell walls had a positive effect on digestibility of S. cerevisiae for Arctic charr.     

Long‐term effects on soil microbial properties of heavy metals from industrial exhaust deposition

Soil samples were taken at 0—10 cm and 10—20 cm depth from 7 clay‐marsh sites used as grassland close to Nordenham in the north of Lower Saxony, Germany. The sites had been contaminated by deposition of heavy metals from industrial exhausts, the level of contamination varying according to their distances from a lead factory. The soils were analyzed to assess the depth‐specific effects of NH₄NO₃ extractable and total amounts of Zn, Pb, and Cu on basal respiration, adenylates, ergosterol, and biomass C estimated by fumigation extraction (FE) and substrate‐induced respiration (SIR). Most of the chemical and biological properties studied decreased with depth, but depth‐specific differences in the relationships between these properties rarely occurred. The biomass C/soil organic C ratio was at a relatively high level, but most consistently reflected pollution as a decrease with increasing heavy metal load, independently of the method used for biomass C estimation. However, the SIR estimates were on average 44 % lower than those of FE, mainly due to pH effects. The metabolic quotient SIR‐qCO₂ increased with increasing NH₄NO₃ extractable and total heavy metal contents, but also with decreasing pH, whereas the FE‐qCO₂ remained unaffected by heavy metals and pH. The ATP/FE‐biomass C ratio was on average 8.2 μmol g^—1 and negatively affected by soil pH, but also by total Zn, NH₄NO₃ extractable Zn and Cu. The ergosterol/FE‐biomass C ratio was on average 0.29 %, i.e. at a very low level, and increased with increasing heavy metal content. This indicates a change in the community structure towards fungi.     

Determination of sampling volumes needed for representative analysis of alpine soils

Alpine soils are characterized by a high variability of chemical and physical parameters. The spatial inhomogeneity of a specific parcel must be regarded as a nuisance factor for representative soil sampling. The only way to reduce the effect of the variation between the samples is to combine several samples taken within an area of a few square meters. The presented approach shows how much soil material has to be taken in order to obtain representative values of soil properties such as soil density, the proportion of soil skeleton and fine earth, organic C, N, and the root content. Bulk density can be determined with an error ≤ 10% by sampling c. 6300 cm³ of soil within an area of 6 × 4.5 m for 0—20 cm sampling depth. With the same volume, the accuracy of soil skeleton measurement is ≤ 20%. In our case study, the determination of organic C in the fine earth of the whole soil profile needs a sample volume of 15,800 cm³ in order to reduce the error tolerance below ± 10%. In 0—20 cm soil depth, the error tolerance of ± 10% is achieved with c. 4000 cm³ for N and c. 6300 cm³ for organic C. Nearly 16,000 cm³ were needed to determine the soil root mass in the top 20 cm of a soil with an accuracy of ± 10%. In order to yield reasonable results, large sampling volumes are needed for soils in alpine areas. These volumes depend on the parameters to be investigated. Our investigation makes an initial attempt at optimizing the sampling strategy within an area of a few square meters in order to overcome this nuisance factor variation.