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141.
Encarnación García Lucas Carlos García Izquierdo Mª Teresa Hernández Fernández 《Journal of Soils and Sediments》2018,18(4):1376-1388
Purpose
The aim of this study was to evaluate in the medium term (5 years) the effect of two organic amendments, which were spiked to a degraded soil as a strategy for bioremediation, on the amount and characteristics of soil humic acids (HAs) and their ability to associate with certain extracellular enzymes.Materials and methods
Soil samples were collected in an experimental field where 5 years earlier, a mixture of the organic fraction of household waste and sewage sludge (2:1 ratio), both composted (composted residue, CR) and non-composted (fresh residue, FR), had been added in triplicate at rates equivalent to 1 % (D1) and 3 % of organic carbon (D2) to 30-m2 plots as a strategy for degraded soil restoration. Humic substances (HSs) and HAs were extracted from the collected soil samples and submitted to chemical, biochemical, spectroscopic (FTIR), and chemical-structural (CPMAS 13C NMR) analyses.Results and discussion
After 5 years, the amended soils showed significantly higher HS and HA content than did the control soil, and the differences with respect to the control were greater with compost addition than with FR addition. The HA from the amended soils had higher H, N, and S contents than the HA from the non-amended soil in addition to a lower oxygen content and lower O/C ratio values. Furthermore, the FTIR spectra of the HA from the amended soils showed a higher absorption intensity in bands corresponding to aliphatic and amide-carboxylic groups and polysaccharide structures and a lower absorption intensity in bands corresponding to carbonyls and carboxylic groups than the HA from the control. These results were confirmed by 13C-NMR spectra, which showed a clear increase of aliphatic compounds in the HA from the amended soils with respect to the HA from the control. HA spectra were not greatly influenced by the maturity of the amendment or by the application dose.Conclusions
In general, the addition of organic amendments increased the quantity of enzymes immobilized in the humic colloid. Furthermore, the addition of the composted residues favored to a greater extent the immobilization of the abovementioned enzymes, which represent a biological reservoir in the soil. This is of great importance since these enzymes possess functional capacity even when the soils are under conditions that are stressful or unfavorable for microbial life. An increase in the quantity of immobilized enzymes such as that observed in amended soils supposes an important improvement in soil quality.142.
Juan Estefanell Javier Roo Rafael Guirao Juan Manuel Afonso Hipólito Fernández‐Palacios Marisol Izquierdo Juan Socorro 《Aquaculture Research》2012,44(1):93-105
The aim of this study was to evaluate growth, biochemical composition and dietary nutrients utilization in Octopus vulgaris fed on four diets based on bogue Boops boops, from different origin and in two presentations: fresh discarded bogue (aquaculture by‐product) (DB‐f), fresh wild bogue (low price trash species) (WB‐f), discarded bogue agglutinated moist diet (DB‐m) and wild bogue agglutinated moist diet (WB‐m). Diets based on DB showed higher lipid content (19–26% dw) than those based on WB (5–6% dw). Octopuses fed on DB‐based diets showed higher growth (1.5–1.9% day?1) and higher protein efficiency ratio (0.64–0.69) than those fed on WB‐based diet (1.1–1.5% day?1 and 0.36–0.37 respectively), which suggests good utilization of dietary lipids and also a possible protein sparing effect by lipids in O. vulgaris. Octopuses fed on diets presented fresh showed a higher growth (1.9–1.5% day?1) and a higher feed efficiency (62–65%) than those fed on agglutinated diets (1.1–1.5% and 52–60% day?1 respectively). Regarding fatty acids, the digestive gland clearly reflected dietary lipid and fatty acid profile, while muscle showed a more stable composition. Low dietary ARA content reflected in octopus tissues, especially in specimens fed on DB‐based diets, which did not seem to affect growth during the experimental period. 相似文献
143.
144.
L. Planes J. Catalán A. Tena J. L. Porcuna J. A. Jacas J. Izquierdo A. Urbaneja 《Journal of pest science》2013,86(2):321-327
Spirotetramat is a new systemic insecticide listed in Group 23 of the IRAC mode-of-action classification scheme as an inhibitor of lipid biosynthesis. Side effects assessment on key natural enemies is necessary before incorporating a pesticide in IPM programs. Herein, lethal and sublethal side effects of spirotetramat on adults and larvae of Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) were evaluated under laboratory conditions by topical application and by ingestion of treated individuals of Planococcus citri Risso (Hemiptera: Pseudococcidae). The lethal and sublethal effects of spirotetramat were compared to those of chlorpyriphos and pyriproxyfen, two insecticides commonly used in Spanish citrus. Spirotetramat resulted harmless: (1) when directly applied on larvae and adults of C. montrouzieri, since it did not affect survival, longevity, fecundity, egg hatching, and offspring survival. In contrast, chlorpyriphos was classified as moderately toxic for adults due to its effects on fecundity, egg hatching and offspring survival. Pyriproxyfen was classified as harmful for larvae due to the acute effect on pupal mortality. When larvae and adults of C. montrouzieri were fed with treated prey, spirotetramat was also classified as harmless. Adults of C. montrouzieri fed with pyriproxyfen-treated prey exhibited increased fecundity but no eggs hatched. Moreover, the larvae fed on pyriproxyfen-treated prey did not reach the adult stage. The results of this study indicate that spirotetramat may be compatible with augmentative releases of C. montrouzieri in citrus. 相似文献
145.
Montero D Socorro J Tort L Caballero MJ Robaina LE Vergara JM Izquierdo MS 《Journal of fish diseases》2004,27(5):297-306
An experiment was conducted to determine the effect of an essential fatty acid (EFA) deficient diet on growth, immune status and renal morphology of juvenile gilthead sea bream, using two diets: a control diet containing 2% (DW) of n-3 high unsaturated fatty acid (n-3 HUFA) and a diet formulated to be deficient in EFA for this species and containing 0.5% DW of n-3 HUFA (diet NFA). After 9 weeks of feeding the EFA-deficient diet fish showed a reduction in growth compared with the control group (107.48 +/- 9.14 and 123.14 +/-11.87 g final weight respectively). Fish fed the NFA diet showed a reduction in the erythrocyte volume together with increased erythrocyte fragility, haemoglobin content and red blood cell count in comparison with fish fed the control diet. EFA deficiency also reduced cellular immunity in terms of neutrophil activity and the number of circulating lymphocytes. The serum alternative complement pathway was markedly reduced in fish fed the EFA deficient diet. Fish fed the control diet showed glomeruli with a well-defined Bowman's space and normal renal tubes. However, up to 88% of fish fed the EFA-deficient diet showed alterations in renal morphology affecting at least 50% of the glomeruli, which showed extreme dilation of capillaries and occlusion of Bowsman's capsule. Mesangial proliferation and diffuse thickening of the capillary walls, as well as renal tube degeneration, were also observed. 相似文献
146.
Influence of dietary polar lipids' quantity and quality on ingestion and assimilation of labelled fatty acids by larval gilthead seabream 总被引:2,自引:0,他引:2
Dietary supplementation of phospholipids seems to be extremely important to promote growth and survival in fish larvae. Several studies also suggest the importance of n-3 highly unsaturated fatty acids (HUFA) rich phospholipids to further enhance larval performance. In the present study, four different diets were formulated in order to compare the effect of total dietary polar lipid contents, of soya bean lecithin supplementation and of feeding n-3 HUFA in the form of neutral or polar lipids on ingestion and incorporation of labelled fatty acids in gilthead seabream larvae. These diets were prepared including radiolabelled fatty acids from palmitoyl phosphatidylcholine, glycerol trioleate, free oleic acid (FOA) and free eicosapentaenoic acid (FEPA) and were fed to 25 day-old larvae. The results of these experiments showed that the elevation of the dietary polar lipid levels significantly improved microdiet ingestion, regardless of the origins of the polar lipids. This effect caused an improved incorporation of phosphatidylcholine fatty acids to the larval polar and total lipids (TL) as the dietary polar lipids increased. Nevertheless, a better incorporation of fatty acids from dietary polar lipids in comparison with that of fatty acids from dietary triglycerides into larval lipids was found in gilthead seabream, whereas a better utilization of dietary triglycerides fatty acids than dietary free fatty acids could also be observed. Besides, the presence of n-3 HUFA rich neutral lipids (NL) significanlty increased the absorption efficiency of labelled oleic acid from dietary triglycerides, but the presence of n-3 HUFA rich polar lipids, particularly improved the incorporation of FEPA. This fatty acid was preferentially incorporated into larval polar lipids in comparison with FOA. 相似文献
147.
Eyad Atalah Carmen María Hernández‐Cruz Eneko Ganuza Tibiabin Benítez‐Santana Rachid Ganga Javier Roo Daniel Montero Marisol Izquierdo 《Aquaculture Research》2011,42(9):1261-1268
Together with docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), arachidonic acid (ARA) is being considered to be an essential fatty acid in marine fish larval diets. The objective of the present study was to determine the importance of dietary ARA levels for larval European sea bass performance, when EPA and DHA are also present in the diet. Eighteen‐day‐old larvae were fed, for 14 days, gelatine‐based microdiets containing the following ARA levels: 0.3%, 0.6% or 1.2%. Elevation of dietary ARA up to 1.2% showed a positive correlation with larval survival and a significant improvement in the specific growth rates, body weight and total length. Arachidonic acid was efficiently incorporated into larval lipids, even at a higher proportion than that in the diets. Increased accumulation of ARA did not affect the incorporation of DHA or EPA from the diet into larval total lipids. A significant positive correlation was found between dietary ARA levels and survival after handling stress, indicating the importance of this fatty acid in sea bass larvae response to acute stressors. The results show the importance of ARA for sea bass larvae, but higher dietary levels should be tested to determine whether there is a negative effect of ARA in sea bass as reported for other species. 相似文献
148.
Linking coastal aquaculture of meagre Argyrosomus regius and Western Mediterranean coastal fisheries through escapes incidents 下载免费PDF全文
P. Arechavala‐Lopez J. M. Valero‐Rodriguez J. Peñalver‐García D. Izquierdo‐Gomez P. Sanchez‐Jerez 《Fisheries Management and Ecology》2015,22(4):317-325
Escape incidents in coastal aquaculture lead to economic losses for farmers and may have indirect socio‐economic effects on local fisheries. In this study, the relationship of meagre, Argyrosomus regius (Asso), production in open‐sea cages and coastal small‐scale fisheries was analysed through captures of escapes, which are easily detected because this species is considered locally absent in native communities in Western Mediterranean regions. Scale reading showed that 100% of captured meagre were escapees. The existence of a direct relationship, in terms of biomass, between the development of meagre coastal aquaculture and the increase of captures of this species by local fisheries was demonstrated. The spatial distribution of meagre captures suggested that there is a local environmental and economic interaction between meagre aquaculture and fisheries through escapees. Monitoring the presence of locally absent species such as meagre within landings might help to assess the magnitude of escapes, the potential economic effects on local aquaculture and fishery industries, and the potential adverse ecological impacts on local ecosystems. 相似文献