Pinus plantations have increased in Brazil, and native forest areas have been converted for timber production. The clearing and the long-term loblolly pine (Pinus taeda L.) land-use effects on soil carbon and nitrogen stocks were evaluated in a natural broadleaved forest and in loblolly pine sites cultivated for 29, 35, 38 and 49 years, as well the soil contribution as ecosystem carbon pool. According to the exponential-decay model fitted to changes in carbon stock, the initial soil carbon stock of 200 Mg ha?1 to a depth of 100 cm in the natural forest decreased by 36% over 49 years of pine cultivation (around 72.4 Mg ha?1 of C). Around two-thirds of this decrease occurred in the top 30 cm of the soil and intensively in the first 12 years of cultivation, but slowly faded as carbon stock tended to reach a new steady state after approximately 49 years of cultivation. The soil nitrogen stock in the natural forest was 14.2 Mg ha?1 to a depth of 100 cm and decreased by 36% over the 49 years. This decrease was linear according to the fitted model, especially in the top 30 cm where nitrogen decline was 83% and was proportionally more intense than the carbon decline. Despite the soil carbon decrease, soil remained the largest carbon reservoir in the ecosystem for the growing rotation time of loblolly pine in this region.
This is a review of the physiology and endocrinology of the estrous cycle and how ovarian physiology can be manipulated and controlled for timed artificial insemination (TAI) in beef and dairy cattle. Estrus detection is required for artificial insemination (AI), but it is done poorly in dairy cattle and it is difficult in beef cattle. Protocols that synchronize follicle growth, corpus luteum regression and ovulation, allowing for TAI, result in improved reproductive performance, because all animals are inseminated whether they show estrus or not. As result, TAI programs have become an integral part of reproductive management in many dairy herds and offer beef producers the opportunity to incorporate AI into their herds. Gonadotropin-releasing hormone-based protocols are commonly used in North America for estrus synchronization as part of a TAI program. Protocols that increase pregnancy rates in lactating dairy cows and suckling beef cows have been developed. Protocols that improve pregnancy rates in heifers, acyclic beef cows, and resynchronized lactating dairy cows are also discussed. 相似文献
Brazil has a herd of 212 million cattle and 171 million hectares of pastures that produce approximately 96 % of Brazilian beef. The Brazilian production system enables animal infection by endoparasites, which are considered one of the main obstacles for the development of this industry and are responsible for considerable economic losses. The control of parasitic diseases is performed via the administration of antiparasitic drugs, but they leave residues of the products in the treated animal, affect non-target organisms and select resistant strains of the parasites. The species D. flagrans and M. thaumasium are promising and sustainable alternatives for controlling gastrointestinal helminths of ruminants and other herbivores. In this study, we evaluated the efficacy of isolates of these species, formulated in a sodium alginate matrix and administered twice a week, to reduce the number of environmental infective larvae of gastrointestinal nematodes that affect prepubescent zebu females. The treated animals presented fewer eggs and a lower number of infective larvae per gram of faeces (p?<?0.05). The pastures occupied by treated animals showed a statistically significant reduction (p?<?0.05) of the number of L3 and, furthermore, the genera Cooperia sp., Haemonchus sp., and Oesophagostomum sp. were the most prevalent. The average weight of the animals did not differ statistically (p?>?0.05) among the treated and control groups. The use of sodium alginate pellets as vehicle for delivery of the fungus mycelia D. flagrans (isolate AC001) and M. thaumasium (isolate NF34A) proved effective in controlling trichostrongylids in prepubescent cows bred in the semi-arid region, with an effective reduction in the number of infective larvae in the pastures. 相似文献
Influenza is a viral disease that affects human and several animal species. In Brazil, H1N1, H3N2 and 2009 pandemic H1N1 A(H1N1)pdm09 influenza A viruses (IAV) circulate in domestic swine herds. Wild boars are also susceptible to IAV infection but in Brazil until this moment there are no reports of IAV infection in wild boars or in captive wild boars populations. Herein the occurrence of IAV in captive wild boars with the presence of lung consolidation lesions during slaughter was investigated. Lung samples were screened by RT-PCR for IAV detection. IAV positive samples were further analyzed by quantitative real-time PCR (qRRT-PCR), virus isolation, genomic sequencing, histopathology and immunohistochemistry (IHC). Eleven out of 60 lungs (18.3%) were positive for IAV by RT-PCR and seven out of the eleven were also positive for A(H1N1)pdm09 by qRRT-PCR. Chronic diffuse bronchopneumonia was observed in all samples and IHC analysis was negative for influenza A antigen. Full genes segments of H1N2 IAV were sequenced using Illumina's genome analyzer platform (MiSeq). The genomic analysis revealed that the HA and NA genes clustered with IAVs of the human lineage and the six internal genes were derived from the H1N1pdm09 IAV. This is the first report of a reassortant human-like H1N2 influenza virus infection in captive wild boars in Brazil and indicates the need to monitor IAV evolution in Suidae populations. 相似文献
The presence of the non-indigenous species, the black-pygmy mussel Xenostrobus securis, is reported here for the first time in an intense shellfish farming area off Galicia (NW Spain). Very high concentrations
of this mytilid bivalve have colonized estuarine waters located at the inner part of the Ria de Vigo. The invasive role of
X. securis is discussed in the context of the wide ecological tolerance of the species and the recent finding of settlements of this
species on numerous colonies of the economically-important blue mussel Mytilus galloprovincialis. The mode of introduction of the black-pygmy mussel is also discussed in relation to human management activities. 相似文献
Landscape and site-scale data analyses aid the interpretation of biological data and thereby help us develop more cost-effective natural resource management strategies. Our study focused on environmental influences on stream assemblages and we evaluated how three classes of environmental variables (geophysical landscape, land use and cover, and site habitat), influence fish and macroinvertebrate assemblage richness in the Brazilian Cerrado biome. We analyzed our data through use of multiple linear regression (MLR) models using the three classes of predictor variables alone and in combination. The four MLR models explained dissimilar amounts of benthic macroinvertebrate taxa richness (geophysical landscape R2 ≈ 35 %, land use and cover R2 ≈ 28 %, site habitat R2 ≈ 36 %, and combined R2 ≈ 51 %). For fish assemblages, geophysical landscape, land use and cover, site habitat, and combined models explained R2 ≈ 28 %, R2 ≈ 10 %, R2 ≈ 31 %, and R2 ≈ 47 % of the variability in fish species richness, respectively. We conclude that (1) environmental variables differed in the degree to which they explain assemblage richness, (2) the amounts of variance in assemblage richness explained by geophysical landscape and site habitat were similar, (3) the variables explained more variability in macroinvertebrate taxa richness than in fish species richness, and (4) all three classes of environmental variables studied were useful for explaining assemblage richness in Cerrado headwater streams. These results help us to understand the drivers of assemblage patterns at regional scales in tropical areas. 相似文献
Abstract.— The tolerance of juvenile flatfish Paralichthys orbignyanus to acid stress, was studied in 30-L plastic tanks using ten flatfishes (95 ± 13 g) per treatment. Water pH was monitored every 3 h and re-adjusted to the test pH using either HCl or NaOH. The photoperiod was fixed at 12L:12D, water temperature at 23 ± 0.8 C, and salinity at 30 ppt. Fish survival and ventilatory rate were monitored daily. At pH 4.0, we observed 100% mortality and ventilation rates different from controls. At pH 5.2, there was 100% survival even after 96 h. However, ventilation rate was higher than in controls. At pH equal or higher than 6.0, we observed 100% survival and normal ventilation rates. The estimated pH which would kill 50% of test organisms were: 4.51, 4.50 and 4.40 after 48, 72, and 96 h of experiment, respectively. These results show that juvenile flatfish are relatively tolerant to acid stress. 相似文献
1 This paper lists 171 freshwater macroinvertebrate taxa from Tenerife (excluding chironomids) based on this study and other published information. Canarian endemism and a Mediterranean origin are dominant features in the data set. At least 14 species are Tenerife endemics. Endemism is especially high in the Diptera, Trichoptera and Coleoptera.
2 Extensive investigations were carried out at 57 sites in six different habitat categories and from at least two seasons. The most species-rich habitats were those with permanently running water, a biotope that is rare and diminishing in Tenerife.
3 The principal threat to freshwater environments is an increase in human exploitation of the water resources, which, if it is not moderated, will cause extinctions in the near future. Taxa that do not favour man-made canals are especially at risk—a risk that is increased by the small number of populations. These include the Canarian endemics Wormaldia tagananana, Tinodes canariensis (Trichoptera), Dixa tetrica (Diptera) and Baetis pseudorhodani (Ephemeroptera). Thirty-nine species were found at five or fewer sites.
4 Some species can survive in intermittent streams, although we found less than half of the taxa present in this habitat, indicating the extent of the diversity reductions that might be expected to follow the drying up of permanent streams.
5 Species living in the thin film of water in the madicolous habitats are similarly at risk. Three Canarian endemics were found exclusively in this habitat: Hydrotarsus pilosus, Limnebius punctatus (Coleoptera) and Satchelliella binunciolata (Diptera).
6 As a first step, protective measures are proposed for five of the most species-rich streams. If these were to be implemented, they would make a significant contribution towards maintaining biodiversity in the fresh waters of Tenerife.
Pesticide formulations based on organoclays have been proposed to prolong the efficacy and reduce the environmental impact of pesticides in soil. This research addressed the question of whether atrazine in organoclay-based formulations is irreversibly sorbed or is bioavailable for bacterial degradation in soil. Different cations of l-carnitine (CAR), tyramine (TYRAM), hexadimethrine (HEXADIM), phenyltrimethylammonium (PTMA), hexadecyltrimethylammonium (HDTMA), and Fe(III) were incorporated into Na-rich Wyoming montmorillonite (SWy-2) and Ca-rich Arizona montmorillonite (SAz-1) at 100% of the cation exchange capacity (CEC) of the clays as a strategy to enhance the affinity of the clay minerals for atrazine. A Buse loam soil from Becker, MN, was treated with three organoclay-based formulations of 14C-atrazine or free herbicide and incubated for 2 weeks. To determine the bioavailability of 14C-atrazine, the soil was inoculated with Pseudomonas sp. strain ADP, which rapidly mineralizes atrazine. At day 0, and after a 2 week incubation, mineralization and the amount of 14C-atrazine residues distributed between the aqueous-extractable, methanol-extractable, and bound fractions in the soil were determined to characterize the availability of nonaged and aged atrazine residues. By the end of the 2 week incubation, the microorganisms had mineralized >80% of the initial readily available (water-extractable) and >70% of the less readily available (methanol-extractable) 14C-atrazine in the soil. Bound residues increased from <4% at day 0 to ~17% after the 2 week incubation for both the formulated and free forms of atrazine. The results of these incubation experiments show that the bioavailabilities of atrazine were similar in the case of the organoclay formulations and as free atrazine. This indicated that whereas more atrazine was sorbed and less likely to be transported in soil, when formulated as organoclay complexes, it was ultimately accessible to degrading bacteria, so that the herbicide is likely to be naturally attenuated by soil microorganisms. 相似文献
We evaluated the use of MALDI-TOF MS for the identification of 3 major, dairy-associated Prototheca species, namely, Prototheca bovis (formerly P. zopfii genotype 2), P. blaschkeae, and P. ciferrii (formerly P. zopfii genotype 1). The MALDI-TOF MS spectra established for those species were introduced into the reference spectra library of the Bruker Biotyper MALDI-TOF MS analysis software. Next, 31 Prototheca isolates from Holstein cows with mastitis, from herds located in the midwestern area of São Paulo State, Brazil, were subjected to MALDI-TOF MS profiling. MALDI-TOF MS allowed identification of 22 of 27 P. bovis and 3 of 4 P. blaschkeae isolates with scores >2.0, with 5 of 27 P. bovis and 1 of 4 P. blaschkeae isolates identified only to the genus level. With our extended algae database, MALDI-TOF MS can contribute to quick and effective speciation of Prototheca from mastitis cases. 相似文献