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41.
Nitrous oxide (N2O) is a high‐impact greenhouse gas. Due to the scarcity of unmanaged forests in Central Europe, its long‐term natural background emission level is not entirely clear. We measured soil N2O emissions in an unmanaged, old‐growth beech forest in the Hainich National Park, Germany, at 15 plots over a 1‐year period. The average annual measured N2O flux rate was (0.49 ± 0.44) kg N ha–1 y–1. The N2O emissions showed background‐emission patterns with two N2O peaks. A correlation analysis shows that the distance between plots (up to 380 m) does not control flux correlations. Comparison of measured data with annual N2O flux rates obtained from a standard model (Forest‐DNDC) without site‐specific recalibration reveals that the model overestimates the actual measured N2O flux rates mainly in spring. Temporal variability of measured N2O flux was better depicted by the model at plots with high soil organic C (SOC) content. Modeled N2O flux rates were increased during freezing only when SOC was > 0.06 kg C kg–1. The results indicate that the natural background of N2O emissions may be lower than assumed by most approaches. 相似文献
42.
Tanzania demarcated three prawn fishing zones along its coast to spread fishing pressure and help the prawn fishery to recover. However, it is unknown whether the demarcated zones correspond to the genetic stock structure of the world's most commercially important prawn, the giant tiger prawn (Penaeus monodon). Thus, this study used partial mitochondrial control region sequences (534 base pairs) to test the hypotheses that (1) giant tiger prawns in Tanzania's demarcated prawn fishing zones are a single stock and (2) the giant tiger prawns on the Tanzanian coast experienced a recent demographic expansion. The sequences showed high haplotype diversity (h = 0.998–1.0) and low nucleotide diversity (θπ = 1.89%–2.24%). The neutrality test and mismatch analysis showed that the hypothesis of recent demographic expansion could not be rejected. The analysis of molecular variance revealed low and insignificant fixation indices between the zones (FST = 0.00025, p > 0.05; ΦST = −0.00027, p > 0.05), suggesting that the three demarcated fishing zones constitute a single stock and that fishers may be targeting the same stock. Furthermore, it was discovered that Zone 2 has the potential to replenish depleted areas; thus, it should be prioritised in future conservation planning. 相似文献
43.
Mammalian fatty acid synthase is a large multienzyme that catalyzes all steps of fatty acid synthesis. We have determined its crystal structure at 3.2 angstrom resolution covering five catalytic domains, whereas the flexibly tethered terminal acyl carrier protein and thioesterase domains remain unresolved. The structure reveals a complex architecture of alternating linkers and enzymatic domains. Substrate shuttling is facilitated by flexible tethering of the acyl carrier protein domain and by the limited contact between the condensing and modifying portions of the multienzyme, which are mainly connected by linkers rather than direct interaction. The structure identifies two additional nonenzymatic domains: (i) a pseudo-ketoreductase and (ii) a peripheral pseudo-methyltransferase that is probably a remnant of an ancestral methyltransferase domain maintained in some related polyketide synthases. The structural comparison of mammalian fatty acid synthase with modular polyketide synthases shows how their segmental construction allows the variation of domain composition to achieve diverse product synthesis. 相似文献
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Determination of the conjugated linoleic acids in cow's milk fat by Fourier transform Raman spectroscopy 总被引:1,自引:0,他引:1
Meurens M Baeten V Yan SH Mignolet E Larondelle Y 《Journal of agricultural and food chemistry》2005,53(15):5831-5835
The collective term "conjugated linoleic acid" or "CLA" generally refers to a mixture of conjugated positional and geometric isomers of linoleic (cis-9,cis-12-octodecadienoic) acid. In nature, these isomers are mainly formed in the rumen by biohydrogenation of polyunsaturated fatty acids. This study concerns a first trial of CLA determination in cow's milk fat by Raman spectroscopy. The spectra of pure cis-9-oleic, cis-9,cis-12-linoleic, cis-9,trans-11-linoleic, and trans-10,cis-12-linoleic acids have been examined in comparison with the spectra of selected milk-fat samples containing between 0 and 3% of CLA. The trial of CLA determination by Raman spectroscopy on cow milk fat has reached its objective with the two following results. First, the examination of the Raman spectra allows to identify three specific Raman signals of the chemical bonds associated to the cis,trans conjugated C=C in the rumenic and trans-10,cis-12-octodecadienoic acids at 1652, 1438, and 3006 cm(-1). Second, the calibration of Raman spectrometer for the CLA determination has indicated that these three specific signals suit very well for the accurate and reliable measurement of CLA concentration in milk fat. To our knowledge, the present study is the first successful attempt to determine the CLA content of milk fat by a spectrophotometric method. 相似文献
46.
The abundance of eastern white pine (Pinus strobus L.) has been significantly reduced across its distribution range over the past few centuries. The species’ regeneration dynamics is well documented in the centre of its range, but is poorly understood at the northern limit of continuous distribution. To address this knowledge gap, we quantified natural white pine regeneration in unmanaged mature stands, identified the most important variables influencing it, and evaluated the impact of damaging agents, namely white pine blister rust (Cronartium ribicola J.C. Fisch.), white pine weevil (Pissodes strobi Peck), and herbivory. We also quantified the influence of remnant stands and residual trees on the spatial distribution of regeneration in logged sites. The results reveal continuous but low recruitment in mature stands. The basal area of balsam fir (Abies balsamea (L.) Miller) had a strong negative effect on white pine regeneration. Regeneration was more abundant than expected on moister substrates, including moss, decaying wood and organic matter. White pine regeneration was noted in recently logged areas, where distance from remnant stands had a significant effect on the abundance of white pine regeneration. The northern limit of continuous distribution holds potential for white pine restoration, for example by preserving remnant white pine stands that can provide seed sources for natural regeneration in a shelterwood cut system, or in adjacent clearcut areas. This study illustrates that different management strategies should be used near northern range limits, where effects of site conditions and disturbance agents are different than in the center of a species’ range. 相似文献
47.
Stone JO Balco GA Sugden DE Caffee MW Sass LC Cowdery SG Siddoway C 《Science (New York, N.Y.)》2003,299(5603):99-102
Surface exposure ages of glacial deposits in the Ford Ranges of western Marie Byrd Land indicate continuous thinning of the West Antarctic Ice Sheet by more than 700 meters near the coast throughout the past 10,000 years. Deglaciation lagged the disappearance of ice sheets in the Northern Hemisphere by thousands of years and may still be under way. These results provide further evidence that parts of the West Antarctic Ice Sheet are on a long-term trajectory of decline. West Antarctic melting contributed water to the oceans in the late Holocene and may continue to do so in the future. 相似文献
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Mohammad Monirul Islam Eef Meerschman Timothy Saey Philippe De Smedt Ellen Van De Vijver Marc Van Meirvenne 《Precision Agriculture》2012,13(3):384-392
Every growing season, paddy fields are kept both flooded and drained for a significant period of time. As a consequence, these
soils develop distinct physico-chemical characteristics. For practical reasons, these soils are mostly sampled under dry conditions,
but the question arises how representative the results are for the wet growing conditions. Therefore, the apparent electrical
conductivity (ECa) of a 1.4 ha alluvial paddy field located in the Brahmaputra floodplain of Bangladesh was measured in both dry and wet conditions
by a sensing system using the electromagnetic induction sensor EM38, which does not require physical contact with the soil,
and compared both surveys. Due to the smooth water surface under wet conditions which ensured increased stability of the sensing
platform, the results of the survey showed considerably reduced micro-scale variability of ECa. Furthermore, the wet survey results more reliably furnished soil-related information mainly due to the absence of soil moisture
dynamics. The differences between ECa under wet and dry conditions were attributed to differences in soil texture, mainly the sand content variation having considerable
effect on soil moisture differences when flooded following drainage. Accordingly, the largest differences between ECa under wet and dry conditions were found in those parts of the field with a large sand content. Hence, the conclusion was
that an ECa survey on flooded fields has an added value to precision soil management. 相似文献