miR-140-3p inhibits viability,invasion and migration of non-small-cell lung cancer A549 cells by targeting PD-L1

AIM: To explore the target relationship between microRNA-140-3p (miR-140-3p) and programmed cell death ligand 1 (PD-L1) and their effect on the viability, migration and invasion of non-small-cell lung cancer A549 cells.METHODS: RT-qPCR was used to detect the miR-140-3p expression in HLF-1, A549 and H1299 cells, and then the A549 cells with the most significant difference were selected as the subsequent research object. TargetScan software and dual-luciferase reporter assay were performed to predict and confirm the target relationship between miR-140-3p and PD-L1. RT-qPCR and Western blot were used to determine the effects of miR-140-3p mimic and inhibitor on PD-L1 expression level. MTT assay was used to detect the viability of A549 cells. Transwell assay was performed to detect the migration and invasion abilities of the A549 cells.RESULTS: miR-140-3p was significantly down-regulated in the A549 cells and H1299 cells (P<0.05). Transfection with miR-140-3p mimic decreased the expression of PD-L1 and inhibited the viability, migration and invasion of the A549 cells. Transfection with pcDNA3.0-PD-L1 reversed the inhibitory effect of miR-140-3p on the viability, migration and invasion of the A549 cells.CONCLUSION: miR-140-3p inhibits the viability, migration and invasion of A549 cells by targeting PD-L1.     

Regulatory effect of astragaloside IV on SDF-1α/CXCR4 in EPCs

AIM: To investigate the effects of astragaloside IV (AS-IV) on chemokine receptor 4 (CXCR4) and stromal cell-derived factor 1α (SDF-1α) in endothelial progenitor cells (EPCs) and its mechanism. METHODS: Rat bone marrow-derived EPCs were cultured in vitro. The proliferation, adhesion, migration, apoptosis and tube formation capacity of EPCs treated with AS-IV and AMD3100, a specific blocker of CXCR4, were observed. The effects of AS-IV on the expression of SDF-1α/CXCR4 at mRNA and protein levels and the protein level of p-CXCR4 in the EPCs were determined. RESULTS: AS-IV significantly enhanced the proliferation, adhesion, migration and tube formation abilities of EPCs, reduced the apoptosis of EPCs, and up-regulated the mRNA and protein expression of SDF-1α and CXCR4 and the p-CXCR4 protein level in the EPCs. On the other hand, AMD3100 blocked the up-regulating effect of AS-IV on the mRNA and protein expression of CXCR4 and the p-CXCR4 protein level in the EPCs, but did not affect the effect of AS-IV on the expression of SDF-1α. CONCLUSION: AS-IV might enhance the biological function of EPCs by regulating the expression of SDF-1α/CXCR in EPCs.     

棉花支持政策对新疆节水灌溉发展的影响

新疆农业用水占到全社会用水量的95%,面对水资源的紧缺,大力发展节水灌溉至关重要。棉花作为新疆种植面积最大的农作物有义务承担节水灌溉的重任。从棉花支持政策入手,运用最小二乘法,分析棉花支持政策对新疆节水灌溉发展的影响,结果发现财政支农投入和信贷支农投入的增加都对新疆节水灌溉发展有一定的促进作用。因此提出加大财政支农和信贷支农的相关对策,以加快新疆节水灌溉发展。     

三峡库区沉积物中重金属化学形态分布特征与相关性分析

为全面了解三峡库区干支流沉积物中重金属的赋存形态,更好地为评价及保护三峡库区水生态环境提供科学依据,于2015年6月在三峡库区采集沉积物样品67个,通过ICP-MS等仪器测定了沉积物中各重金属元素的不同赋存形态量,运用SPSS、Matlab统计分析软件对重金属赋存形态进行对比分析。结果表明,库区表层沉积物中Cr、Ni元素主要以残渣态为主(占总量的75%以上);Cu、Zn、As元素主要以残渣态和可还原态为主(合计占总量80%~95%);Cd元素主要以酸可提取态和可还原态为主(分别占总量的55%和23%);Pb元素主要以可还原态和残渣态为主(各占总量的71%和18%)。Cd和Pb元素生物可利用态量较高,均超过该元素的长江沉积物背景值;该种形态下的重金属元素容易进入水体,并且在地球化学条件变化时转化成能被生物吸收和利用的形态,对生物造成较大危害。干流上游段重庆主城区及下游段,渠系河、大宁河、磨刀溪、沿渡河应当是重金属生态危害研究与防治的重点区域。     

生物菌肥和钾肥配施对苹果钾素吸收及果实品质的影响

为了研究生物菌肥和钾肥对苹果果实品质及生长发育的协同作用,以7 a生'瓦里短枝'(Vallee spur Del)为研究对象,设置T1(K2O 420 kg·hm-2)、T2(生物菌肥2520 kg·hm-2)、T3(生物菌肥2520 kg·hm-2+K2O 294 kg·hm-2)、T4(生物菌肥2520 kg·hm...     

3种药物对兴凯湖翘嘴鲌幼鱼的急性毒性试验

为了解烟酰苯胺、硫酸铜和扑草净对兴凯湖翘嘴鲌幼鱼的毒性影响,在水温(28±1)℃下,采用半静水式试验法开展了这3种药物对体质量(2.88±0.34)g、全长(7.30±0.30)cm的兴凯湖翘嘴鲌幼鱼的急性毒性试验。试验结果显示,烟酰苯胺对兴凯湖翘嘴鲌幼鱼的24、48、72、96 h半致死浓度分别为5.729、4.041、2.224、1.257 mg/L,安全浓度为0.126 mg/L;硫酸铜对幼鱼的24、48、72、96 h半致死浓度分别为3.187、2.116、1.354、0.984 mg/L,安全浓度为0.098 mg/L;扑草净对幼鱼的24、48、72、96 h半致死浓度分别为0.072、0.037、0.020、0.012 mg/L,安全浓度为0.001 mg/L。结果表明,烟酰苯胺和扑草净对翘嘴鲌幼鱼的毒性较大,应避免其污染养殖水体,硫酸铜在其常用质量浓度范围内会对兴凯湖翘嘴鲌幼鱼的成活率产生影响,应严格控制使用浓度并规范使用方法。     

乙醇脱氢酶应用研究现状

乙醇脱氢酶(ADH)是广泛分布于人和动物肝脏、植物及微生物细胞之中的含锌金属酶,具有广泛的底物特异性.ADH作为生物体内主要短链醇代谢的关键酶,近年来已经受到了国内外研究者的普遍关注,并对其同功酶的结构、理化性质、生物学功能、遗传学特性、分离提取技术等进行了较多的基础性研究工作,为其在科学研究和工农业生产中的应用奠定了良好基础.文章主要对ADH在工业分析、化工生产、食品和医药研究以及生物科学中的应用进行了概述,以便为其进一步研究开发提供参考.     

南方地区中国荷斯坦牛乳中体细胞数变化规律的研究

采用最小二乘法分析了南方地区某奶业集团2006年7个牛场2 063头中国荷斯坦牛22 377次乳中体细胞数(SCC)测定日记录,以揭示SCC的变化规律.结果表明乳中SCC为459.49×103士924.66×103个/mL,不同牛场、月份、泌乳月和胎次对乳中体细胞数的影响均达到极显著水平(P＜0.01),而产犊季节对乳中SCC的影响未达到显著水平(P＞0.05).SCC随着胎次和泌乳月的增加有升高的趋势.     

不同滴灌条件下土壤水分分布与运移规律

通过对相同规格的滴灌带在不同土壤含水量、不同滴灌方式和不同灌水量条件下灌水,研究水分在土壤中的下渗分布规律。结果表明,单管滴灌条件下,水分在不同含水量土壤中湿润体均呈坛状;双管滴灌条件下,水分在不同含水量土壤中湿润体依灌水量大小而异,灌水量较小时呈并放双碗状,随灌水量增大,湿润体逐渐呈坛状。不同滴灌方式下灌水,满足植物根系需要的灌水量不同,单双管滴灌灌水量分别为300 m3/hm2和450 m3/hm2时即可满足植物根系对水分的需求。单双滴在相同灌水量条件下,土壤表层湿润半径大小变化因土壤含水量不同而不同,土壤垂直湿润深度随灌水量增大而增加;在相同灌水量条件下,滴灌方式和初始土壤含水量对土壤水分湿润圈大小有很大影响。     

跌扩型底流消能工坎后立轴漩涡水力特性分析

跌扩型底流消能工坎后立轴漩涡直接影响消力池内的水力学指标。采用水力学试验方法,对跌扩型底流消能工坎后立轴漩涡水力特性进行初步研究。详细分析了立轴漩涡的运动形式以及运动区域的变化,并对漩涡与底板和边墙切点处流速的变化、漩涡区相对于消力池整体压力的变化情况进行了研究。经试验知,立轴漩涡对消力池内水力学指标影响较大。