合生素对肉仔鸡生长性能的影响

为探讨合生素在肉仔鸡生产中的最适添加量及其对肉仔鸡生产性能的影响,本文采用0.05%和0.10%两个比例添加合生素,其余3组为益生素组、抗生素组和对照组。对6000只AA公雏鸡进行为期7周的试验。结果表明:合生素组(0.05%和0.10%)的平均日采食量、日增重和饲料转化率都优于益生素组和抗生素组,但差异不显著(P>0.05);平均采食量(0￣3、4￣6、6￣7周龄)和平均日增重(0￣3、6￣7周龄)显著高于对照组(P<0.05);添加0.05%的合生素组与0.10%的合生素组对肉仔鸡生产性能有相同的效果(P>0.05)。说明由益生素与植物提取物组成的合生素可以替代抗生素和益生素在肉仔鸡生产中应用,其最适的添加量为0.05%。     

饲料粮比重加大中国的粮食安全性会更高

一、关于粮食问题的几个判断1、口粮消费总量将维持稳定。在相当长一个时期,中国的口粮消费总量,基本维持稳定。随着人们生活水平的提高,城乡口粮直接消费下降。1981年城乡居民人均口粮消费分别为145公斤和256公斤;2005年城市居民口粮消费只有77公斤,农民全年平均口粮消费208.9     

蒽酮-硫酸比色法测定天然鸽乳中可利用糖含量的研究

采用蒽酮-硫酸比色法测定玉米中的可利用糖含量以确定试验测定的最佳条件,并以此条件为对照测定天然鸽乳中可利用糖含量.玉米及天然鸽乳可利用糖溶液经乙醚去脂,高峰氏糖化酶水解,乙醇提取,饱和乙酸铅脱色后制得.结果表明,葡萄糖标准溶液的测定量在0.1～0.6 mg/mL的浓度范围内有良好的线性关系,其回归方程为y=1.6771x 0.0737,相关系数R=0.9987.测定不同的天然鸽乳样品,试验精密度高,重复性好,其可利用糖含量,0～3日龄天然鸽乳为7.45%～7.64%,4～6日龄天然鸽乳为32.08～60.28 %.     

中药渣及添加剂对幼兔增重效果的研究

本试验利用药厂生产“增长乐”取汁后的中药渣，配合于家兔日粮中并对其生长效果进行观察研究。在小白鼠预试的基础上，添加３％的中药渣并配合２００ｐｐｍ铜、７５ｐｐｍ喹乙醇，２００ｐｐｍ铜＋０．３克四环素于幼兔日粮中。结果表明；添加３％中药渣较对照组提高日增重９．６％，节料７％；配合使用２００ｐｐｍ铜，则效果更佳。总的结果表明：中药渣及添加剂作为一种促生长剂增加到家兔日粮中是可行的     

小尾寒羊白介素1β全长cDNA序列克隆及生物信息学分析

本研究旨在克隆白介素1β(IL-1β)全长cDNA,为深入研究其生物学功能提供全长cDNA信息和试验材料.本试验利用前人构建的布鲁氏菌强、弱毒株感染小尾寒羊白细胞层抑制性差减杂交(SSH)cDNA文库,获得小尾寒羊IL-1β部分cDNA序列信息,利用cDNA末端快速扩增技术(RACE),克隆小尾寒羊IL-1β全长cDNA,并申请GenBank登录号KC425612.2.该cDNA全长1494 bp,含有801 bp开放阅读框(ORF),可编码266个氨基酸残基,预测其编码蛋白分子质量为30.622 ku,与林麝、牛、海豚、虎鲸的IL-1β一致性较高,亲缘关系较近.     

枯草芽孢杆菌制剂在奶牛生产上的应用研究

选取生理状况基本相似的黑白花奶牛24头,对照组A,试验组B、C、D,分别在基础日粮的水平上添加不同剂量的枯草芽孢杆菌微生态制剂,分别统计不同时期各组的产奶量和奶成份。结果表明,试验组B、C、D产奶量分别增加3.07%、23.30%和19.80%,C组、D组与对照组差异极显著(P<0.01);试验期间试验组比同期对照组脂肪增加,经t检验D组差异显著(P<0.05);蛋白质在试验后比试验前增加,其中D组增长3.09%,C组增长0.7%。     

Oxalate-degrading capacities of lactic acid bacteria in canine feces

In this study, lactic acid bacteria in canine feces were isolated and identified, and their oxalate-degrading capacities were evaluated. The oxalate-degrading capacities were determined for 24 of 47 (51.06%) lactic acid bacteria isolates. Of these, 8 isolates [Leuconostoc mesenteroides (RL75), Lactococcus garvieae (CD2), Lactococcus subsp. lactis (CS21), Enterococcus faecium (CL71 and CL72), and Enterococcus faecalis (CD14, CS62, and CD12)] degraded more than 5% of the oxalate present, while the others degraded less than 5% of the oxalate in vitro. Isolates that degraded more than 5% of the oxalate present were selected for further examination. The oxalate-degrading capacities of individual isolates, a mixture of Enterococcus, a mixture of Lactococcus, and a mixture of the eight isolates were evaluated in media containing different concentrations of glucose (sufficient, insufficient, or no glucose). In comparison with the control medium, all of the individual isolates and mixtures of isolates could degrade oxalate in all three groups (P<0.05). In most cases, the isolates growing in medium with 20 g/L of glucose had higher oxalate-degrading capacities than those growing in medium with 2.5 g/L of glucose or no glucose. The mixture of all isolates showed higher oxalate-degrading capacity than the individual isolates and other mixtures. The oxalate-degrading capacities of the isolates were isolate dependent.     

应用Excel软件分析敌敌畏农药对两广二号家蚕的安全间隔期

应用Excel软件中的曲线方程分析判断敌敌畏农药对两广二号家蚕的安全间隔期。结果表明：敌敌畏1000倍液的安全间隔期是6d多,其数学模型为幂函数曲线方：y=88．0653x-1834本方法克服以往家蚕的农药安全期无数学模型可循或使用的BASIC方法陈旧繁杂的缺点,也避免英文统计软件SPSS难以在农村普及的不足,是一种计...     

β-carotene attenuates weaning-induced apoptosis via inhibition of PERK-CHOP and IRE1-JNK/p38 MAPK signalling pathways in piglet jejunum

Weaning may cause oxidative injury, immune response impairment, apoptosis and other injuries in piglets. Oxidative and endoplasmic reticulum stress (ERS) can elicit inflammatory responses, and persistent oxidative and ERS also may lead to apoptotic cascades, which is associated with the pathogenesis of multiple diseases. β-carotene, a natural carotenoid, has potential anti-inflammatory and antioxidant functions. However, the effect of β-carotene on apoptosis in weaned piglets and the detailed molecular mechanism remain unclear. In this study, we found that β-carotene decreased malondialdehyde (MDA) levels and increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in piglet serum. β-carotene could inhibit the mRNA levels of caspase-3 significantly, but had no significant inhibitory effect of the mRNA levels of caspase-9 and caspase-12 in the piglet jejunum. In addition, β-carotene decreased the activation of GRP78, CHOP, and JNK/p38 MAPK and the ratio of Bax/Bcl-2. Furthermore, β-carotene had a significant influence on the activation of ERS and apoptosis-related signals in TG-induced IPEC-J2. In the present study, β-carotene pre-treatment attenuated the ratio of Bax/Bcl-2 and prevented TG-induced increases in the level of PERK-CHOP and IRE1-JNK/p38 MAPK pathway activation in a dose-dependent manner. Overall, these findings indicate that β-carotene may protect weaning-induced apoptosis through inhibiting ERS.     

微生态制剂对仔鸡生产性能和免疫功能的影响

研究表明，用不同的微生态制剂饲喂仔鸡，均能促进免疫器官的生长发育，提高免疫器官指数；能明显提高循环抗体的水平和红细胞的免疫功能，因而能促进仔鸡的生长发育，降低料肉比。