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251.
Sunscreen and sunblock are crucial skincare products to prevent photoaging and photocarcinogenesis through the addition of chemical filters to absorb or block ultraviolet (UV) radiation. However, several sunscreen and sunblock ingredients, mostly UV filters, have been associated with human and environmental safety concerns. Therefore, the exploration and discovery of promising novel sources of efficient and safer compounds with photoprotection-related activities are currently required. Marine invertebrates, particularly their associated microbiota, are promising providers of specialized metabolites with valuable biotechnological applications. Nevertheless, despite Actinobacteria members being a well-known source of bioactive metabolites, their photoprotective potential has been poorly explored so far. Hence, a set of methanolic extracts obtained from Cliona varians-derived actinomycetes was screened regarding their antioxidant and UV-absorbing capacities (i.e., photoprotection-related activities). The active extract-producing strains were identified and classified within genera Streptomyces, Micrococcus, Gordonia, and Promicromonospora. This is the first report of the isolation of these microorganisms from C. varians (an ecologically important Caribbean coral reef-boring sponge). The in vitro cytotoxicity on dermal fibroblasts of oxybenzone and the selected active extracts revealed that oxybenzone exerted a cytotoxic effect, whereas no cytotoxic effect of test extracts was observed. Accordingly, the most active (SPFi > 5, radical scavenging > 50%) and nontoxic (cell viability > 75%) extracts were obtained from Streptomyces strains. Finally, LC-MS-based characterization suggested a broad chemical space within the test strains and agreed with the reported streptomycetes’ chemodiversity. The respective metabolite profiling exposed a strain-specific metabolite occurrence, leading to the recognition of potential hits. These findings suggest that marine Streptomyces produce photoprotectants ought to be further explored in skincare applications. 相似文献
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253.
Manuel Soto-Dávila Danixa Martinez Ricardo Oyarzún Juan P. Pontigo Carolina Vargas-Lagos Francisco J. Morera Julia Saravia Fábio Zanuzzo Luis Vargas-Chacoff 《Journal of fish diseases》2020,43(1):111-127
Pathogen interactions with cultured fish populations are well studied, but their effects on native fishes have not been characterized. In Chile, the disease caused by bacterial species Piscirickettsia salmonis represents one of the main issues and is considered to be one of the important pathogens in the field of aquaculture. They have been found to infect native fish. Therefore, it is necessary to understand the impact of P. salmonis on native species of local commercial value, as well as the potential impact associated with the emergence of antibiotic-resistant strains of P. salmonis. Due to this purpose, the native fish Eleginops maclovinus was used in our study. Fish were randomly distributed in tanks and intraperitoneally inoculated with two strains of P. salmonis. No mortality was recorded during the experiment. Cortisol, glucose and total α-amino acid levels increased in fish injected with AUSTRAL-005 strain compared to sham-injected and LF-89-inoculated fish. Moreover, results showed an increase in the activity of carbohydrates and lipids metabolism in liver; and an increase in the carbohydrates, lipids and total α-amino acid metabolism in muscle after injection with AUSTRAL-005. Our results suggest that P. salmonis modulates the physiology of E. maclovinus and the physiological impact increase in the presence of the antibiotic-resistant strain AUSTRAL-005. 相似文献
254.
Luis Daniel Jimnez‐Martínez Dariel Tovar‐Ramírez Carlos Alfonso lvarez‐Gonzlez Emyr Pea‐Marín Susana Camarillo‐Coop Rafael Martínez‐García Elena Palacios‐Mechetnov Rosario Martínez‐Yaez Bartolo Concha‐Frías 《Aquaculture Research》2020,51(7):2629-2640
Five experimental diets containing different lipid sources, fish oil (D1), soybean lecithin (D2), corn oil (D3), canola oil (D4) and olive oil (D5), were evaluated in Atractosteus tropicus larvae for the relative gene expression of the enzymes fatty acid synthase (fas), acetyl‐CoA carboxylase 1 (acc1) and carnitine palmitoyltransferase 1C (cpt1c), in addition to their effects on larval growth, survival and cannibalism during a 30‐day feeding trial. Higher growth and survival were obtained in treatments D1 and D2, and lower performance in diets D3, D4 and D5. The highest levels of expression of fas and acc1 occurred in larvae fed with D1, which contained high amounts of n‐3 long‐chain polyunsaturated fatty acids (LC‐PUFA), mainly DHA and EPA FA are regulators of lipogenesis. The higher cpt1c expression in plant‐based diets is attributed to the fact that these diets are rich in α‐linolenic acid (ALA) and low DHA, EPA and ARA levels that favour ß‐oxidation. In conclusion, the diets with fish oil (D1) and soybean lecithin (D2) were the best treatments for larval growth, survival and cannibalism and thus appear to meet both lipid and energy requirements of A. tropicus larvae, meanwhile the use of vegetable oils influences the expression of intermediary lipogenic genes. 相似文献
255.
Luis Artur Vales Bezerra Matheus Oliveira Freitas Vanessa Salete Daga Thiago Vinicius Trento Occhi Larissa Faria Ana Paula Lula Costa Andre Andrian Padial Viviane Prodocimo Jean Ricardo Simes Vitule 《Fish and Fisheries》2019,20(4):620-639
While environmental alterations have made Homo sapiens the hyperkeystone species of the globe, biotic homogenization initiated a new era, the “Homogenocene.” Still, some terrestrial and aquatic ecosystems in South America are considered pristine and wild, which can lead to a general faith that economic progress is consistent with conservation strategies, even without scientific support. We compiled anthropogenic threats to fish biodiversity in a hierarchical meta‐analysis, along with an evidence synthesis of threats related to biological invasions, based on peer‐reviewed research with the aim to represent the actual conservation status of the South American ichthyofauna. We highlighted human‐related threats and synergistic effects of biological invasions, climate change, environmental alterations (e.g., pollution, aquaculture and damming) and fisheries. Considering measures that reinforce novel alien fish (e.g., artificial hybrids or genetically modified) introductions, it became clear why an eventual increase in local or regional species richness is not always beneficial to aquaculture, biodiversity, human well‐being or nature. In fact, citizens in all societal roles, including scientists, should revise their concepts about threats to fish biodiversity. Environmental policies require more than taxonomic diagnostics to achieve conservation goals under an incompatible scenario of a multiplying number of fish species and biotic homogenization. We advocate for countries in South America using science‐based strategies useful to maintain their social and economic growth along with their “remaining nature.” We live a crucial moment when the government overlooks threats to biodiversity and uses agribusiness as the most acceptable manner of fuelling the economy. 相似文献
256.
Vitamin A (VA) is an essential dietary nutrient for fish and plays an important role in a range of physiological processes including vision, reproduction, embryogenesis, growth and differentiation and maintenance of epithelial cells. Knowledge of effects of VA on aquatic organisms is limited, but new information continues to be published. The aim of the present review is to present an overview of the dietary requirements and functions of VA in fish of commercial importance in aquaculture production. The effects of VA on reproduction and early embryonic development are described, and the estimated dietary requirements for VA of several species of fish are discussed. New research findings on how VA requirements vary with the life stages of fish are explored that will expand understanding of the complex role of VA. Sources of VA in feeds are described, and the potential need to consider how changes in fish feed formulations towards higher use levels of sustainable ingredients may affect dietary VA supplementation recommendations is discussed. 相似文献
257.
258.
Celia Barbero-Sierra Manuel Ruíz Pérez María José Marqués Pérez Ana María Álvarez González José Luis Cruz Maceín 《Arid Land Research and Management》2018,32(1):111-129
Farmers’ land management is a key factor for sustainable agriculture, yet the traditional knowledge which constitutes its base is seldom incorporated in the land and soil academic literature. The need for a hybrid approach that combines local (farmers’) and scientific knowledge is formally recognized. Plot quality criteria are a component of both sources of knowledge, playing an important role in shaping land management practices. Yet there are few case studies comparing the criteria used by farmers and soil scientists when assessing plot quality, particularly in developed countries. We compare the plot quality assessment done by a sample of 31 farmers in a semiarid area of Spain with an edaphic quality index (EQI) obtained through laboratory analyses of soil samples from 61 farm plots. There is a coincidence between farmer and laboratory assessments in 70% of cases. Plots identified by farmers as being of good quality have a significantly higher EQI than those identified as bad quality. The two key differentiating parameters were clay content (average 21% in good versus 18% in bad quality plots) and soil organic carbon content (1.6 and 1.3%, respectively), both closely related to soil water retention ability. A holistic land vision, a higher level of education and large differences in EQI between good and bad plots as assessed by farmers have a positive influence in the degree of coincidence between farmers’ and laboratory assessments. 相似文献
259.
Eduardo Luis Cupertino Ballester Fabrício Martins Dutra Tito Luís Pisseti Ronaldo Olivera Cavalli Paulo César Abreu Wilson WasieleskyJr 《Aquaculture International》2018,26(3):713-726
The objective of the present work was to determine the influence of artificial substrates that increase the area for biofilm development on the production performance of Farfantepenaeus paulensis juveniles in pens. Shrimp were stocked at a density of 20/m2 in pen structures (bottom area?=?50 m2) that were installed in the Patos Lagoon estuary. Two treatments with three repetitions were analyzed, where artificial substrates (polyethylene nets—1-mm mesh size) were added to increase the area for biofilm development by 100%, and where no substrates were added. During the experimental period, the biomass and the composition of the biofilm were assessed. After 86 days of rearing, no significant differences were found in shrimp performance between the treatments (p?>?0.05). However, the examination of the chlorophyll a, dry weight, and composition of the biofilm indicated that the shrimp were actively consuming the biofilm attached to the artificial substrates. Significant decreases in the abundances of nematodes >?500 μm after the 56th day and of tintinnids and rotifers between day 28 and day 42, indicated that the shrimp were selectively predating on these organisms. Moreover, a decrease in the chlorophyll a concentration in the biofilm suggests that the shrimp were consuming the microalgae. Although the increase in the area for biofilm development did not improve shrimp performance, the shrimp presented the highest growth rates when they consumed most of the biofilm microorganisms. 相似文献
260.
Roselene Nogueira Rodrigues Marcos Sidney Brito Oliveira Marcos Tavares-Dias 《Journal Of Applied Aquaculture》2018,30(1):29-38
Fish farming has grown rapidly in Brazil over the last two decades, due in part to the availability of water and species with potential for cultivation, such as Colossoma macropomum. This farmed fish, however, can be affected by parasitic infections with high mortality rates (>35%). The main objective of the present study was to describe the first occurrence of the Trypanosoma species in Colossoma macropomum, which occurred in fish cultured in the Brazilian Amazon region, as well as to morphologically characterize the trypomastigotes and the impact of these hemoparasites on the body condition of the hosts. The trypanosomes found in the blood of Colossoma macropomum were morphologically characterized by the size of the trypomastigote form, indicating the presence of only one morphotype. Of the 39 hosts examined from one fish farming, 41.0% were infected by Trypanosoma sp., with low levels of infection (mean blood density of 3.7/μL). The condition factor of the asymptomatic hosts was not affected by hemoparasitism. There was no correlation between the abundance of Trypanosoma sp. and the condition factor and size of the hosts. Finally, our understanding of host-parasite interactions and the detection of emerging diseases are fundamental for aquaculture. 相似文献