Fertilizing capacity of the ejaculate of nutria (Myocastor coypus) after the removal of the seminal vesicles as evaluated by the penetration test and natural mating

The fertility of male coypu sperm following seminal vesicle extirpation was investigated using the penetration test into the egg of Syrian golden hamster (Mesocricetus auratus). Ejaculates were obtained from five males by means of electro-ejaculation under halothane narcosis. The results of the zona-free hamster eggs (ZFHE) penetration test showed that the ejaculates of all the surgically treated coypu males were fertile and that ZFHE value fluctuated from 54 to 76.6%. The results obtained in experiments with natural mating revealed that the extirpation of male coypu seminal vesicles did not affect their fertility. In total 47 foetuses were found post mortem in ten coypu females covered by surgically treated males, which on average represented 4.7 foetuses per female.     

Analysis of In vitro Fertilizing Capacity to Evaluate the Freezing Procedures of Boar Semen and to Predict the Subsequent Fertility

A porcine in vitro fertilization (IVF) system and seminal quality parameters of frozen–thawed boar semen were used to assess the effectiveness of two different thawing rates of frozen boar semen, and to address the question of whether differences between fertility of ejaculates could be predicted in a limited field trial. In the first experiment, two thawing procedures were analysed (37°C, 30 s; 50°C, 12 s) and no differences in sperm quality were found. However, when the procedure was 50°C, 12 s the IVF results showed a higher number of sperm per penetrated oocyte and a near 10 points higher rate of pronuclear formation. In the second experiment, the fertility results obtained in the limited field trial show to be efficient enough for application in a commercial use, especially for three of the employed boars (fertility ≥80%). In this limited study, the conventional seminal parameters are not accurate enough to discriminate good and bad boars in relation to fertility. On the contrary, parameters of in vitro penetrability are more precise to predict subsequent fertilities. As conclusion, the IVF fertilization system seems to be a good tool to evaluate the quality of frozen–thawed boar semen previous to its commercial way, to verify the bank semen storage quality and a good way to assay new sperm freezing procedures, as it is the more precise evaluating method in estimating the potential fertilizing ability.     

规模猪场母猪繁殖障碍综合征的病因调查

作者采用9套试剂盒，检测了7种能引起猪繁殖障碍综合征的传染病，对来自有繁殖障碍症状的305场次进行猪瘟(HC)抗原检测，并对包括这305场次在内的978个猪场(次)病例的6346份血清样品及52698份田间血清样品进行HC、猪繁殖与呼吸综合征(PRRS)、伪狂犬病(PR)、日本乙型脑炎(JE)、细小病毒感染(DPV)、猪衣原体病(Chla)、布鲁氏菌病(Bruc)等7种传染病的抗体检测。结果表明，在有繁殖障碍症状的猪场，HC抗原检出率高达61．97％，并且在不使用疫苗的情况下，PR3RS、PR、JE、Chla和PPV的抗体阳性场分别为49．42％、34．29％、12．72％、31．71％、和48．08％。HC和：PR交叉感染率达23．81％；HC和：PRRS交叉感染率为9．52％，PR和PRRS交叉感染率高达59．65％；另外JE、PPV也同HC、PR、PRRS存在部分交叉感染。不使用疫苗的田间血清样品的PRRS、PR的抗体阳性率也高。HC与PRRS．PR、Chla、JE和PPV中的一种或几种混合感染可能是引起繁殖障碍造成严重损失的主要原因。加强综合防制，优化免疫程序、把握引种关、加强生物安全措施是防制猪繁殖障碍综合征的关键。     

2009年4月全国饲料生产形势分析

1全国总体概况 4月份，国内畜禽产品消费增加，禽类产品价格有所回升，饲料产量继续增长，水产饲料增长幅度尤为明显。根据对全国29个省（自治区、直辖市）167家饲料企业的调查显示，其总产量为270．4万t，同比上涨5．1％，环比上涨12．2％（表1）。     

生姜对鸡生长性能及血液生化指标的影响

用1日龄健康地方鸡300只按完全随机设计分成3个处理组,每组100只,分别饲喂添加新鲜生姜粉1％(处理2)、喹乙醇80mg/kg(处理3)、不添加生姜和喹乙醇,(处理1,对照组)的日 粮,观察至60日龄。添加生姜组的日增重、饲料增重比和成活率明显优于对照组(P<0.05)。血清 胆固醇含量较对照组和喹乙醇组均低,血清总蛋白含量较对照组和喹乙醇组略高。     

Estimating Combining Ability of 4x-2x Crosses in Cultivated Potatoes

Progenies derived from crosses between Solarium tuberosum and 2n pollen-Producing diploid hybrids, exhibit obvious hybrid vigor. The 2n pollen-producing clone can act as a bridge in crossing S. tuberosum and S. andigena with S. phureja. Populations from 4x-2x crosses show more unifomity and less segregation compared with that of 4x-4x crosses. The parent-offspring correlation for the traits, starch content and tuber number, is significant at 0.01 level. The regrssion equations are Y (mp-F1)=1.0 1.2x and Y (mp - F1) = 5.3 0.8x, respectively. The 2n pollen-producing clones play an impotant role in increasing tuber stach content. Estimates of the combining ability for the main yield components indicate that additive effect prodominatcs for such trais as plot yield, tuber weight per plant and starch content, whereas both additive and non-additive effects lay equal stress on mean tuber weight and non-additive effect is important for tuber number. In general, non-additive effect appears to be important in     

江苏毛竹林生产力类型区的划分及分析

根据毛竹生产力和环境指标将江苏省毛竹林区划分出高生产力、中生产力、一般生产力和低生产力类型区,并分析了各类型区的主要特点。同时,提出了各类型区的经营发展方向。     

甘薯块根组织感染黑斑病时产生的自发荧光现象及其应用

 1986、87两年分别采用上一年无病种薯28个品种/系各3块,取中部切决于切面上蘸接近等量和等浓度的黑疤病(Ceratocystis fimbriata)孢子悬液,恒温、恒湿培养,定期取样作侵染区组织徒手切片、乳酚油封片用BHZ型0lympus荧光显微镜(B+G滤镜激发)观察,取得以下资料。     

夏大豆亩产262.1kg生理指标研究

研究了夏大豆中油－８９Ｄ亩产２６２．１ｋｇ各项生理指标：叶面积系数，开花期、结荚期、鼓粒期分别为３．９８、５．８０、４．９１；总光合势１８．２万ｍ＾２·日，平均净光合生产率４．８１ｇ／ｍ＾２·日，氮磷总积累量分别为２２．２８ｋｇ和３．１３ｋｇ，每生产１００ｋｇ籽粒需吸收氮８．５０ｋｇ，磷１．１９ｋｇ，总干物质积累８７６．４７ｋｇ，粒茎比０．５１，经济系数０．３０。