全文获取类型
收费全文 | 11720篇 |
免费 | 815篇 |
国内免费 | 4篇 |
专业分类
林业 | 999篇 |
农学 | 482篇 |
基础科学 | 91篇 |
3078篇 | |
综合类 | 355篇 |
农作物 | 553篇 |
水产渔业 | 1242篇 |
畜牧兽医 | 4507篇 |
园艺 | 215篇 |
植物保护 | 1017篇 |
出版年
2023年 | 90篇 |
2022年 | 153篇 |
2021年 | 289篇 |
2020年 | 286篇 |
2019年 | 254篇 |
2018年 | 584篇 |
2017年 | 539篇 |
2016年 | 538篇 |
2015年 | 423篇 |
2014年 | 484篇 |
2013年 | 730篇 |
2012年 | 849篇 |
2011年 | 801篇 |
2010年 | 487篇 |
2009年 | 432篇 |
2008年 | 681篇 |
2007年 | 678篇 |
2006年 | 531篇 |
2005年 | 540篇 |
2004年 | 466篇 |
2003年 | 400篇 |
2002年 | 354篇 |
2001年 | 254篇 |
2000年 | 264篇 |
1999年 | 215篇 |
1998年 | 50篇 |
1997年 | 50篇 |
1996年 | 45篇 |
1995年 | 40篇 |
1994年 | 23篇 |
1993年 | 26篇 |
1992年 | 72篇 |
1991年 | 54篇 |
1990年 | 63篇 |
1989年 | 61篇 |
1988年 | 44篇 |
1987年 | 55篇 |
1986年 | 54篇 |
1985年 | 54篇 |
1984年 | 43篇 |
1983年 | 45篇 |
1982年 | 26篇 |
1979年 | 36篇 |
1978年 | 35篇 |
1977年 | 36篇 |
1974年 | 29篇 |
1973年 | 48篇 |
1972年 | 34篇 |
1971年 | 27篇 |
1969年 | 23篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
961.
962.
Ingemar Alenäs B. Ingvar Andersson Hans Hultberg Arno Rosemarin 《Water, air, and soil pollution》1991,59(1-2):55-77
Long-term monitoring, 1973 to 1987, of reactions to liming and reacidification of a forest lake ecosystem near the Swedish west coast is reported in this study. Treatment of Lake Lysevatten with a slag product of limestone in 1974 resulted in neutralization and a positive alkalinity. Prolonged dissolution proceeded for about 7 yr whereby 86% dissolved. During 1984–86 Lake Lysevatten approached maximum reacidification with high Al concentrations and an affected biota. Asellus aquaticus L. decreased and dominance within chironomid groups approached preliming conditions. However, the most obvious biological change was the development of the filamentous algal genus Mougeotia and increased growth of Sphagnum. Populations of both plants increased notably when pH declined to about 5. Our study suggests that extensive reacidification (pH < 6.0) of limed lakes should be avoided by successive treatments to prevent development of destabilized lake ecosystems. 相似文献
963.
Velasco J Marmesat S Berdeaux O Márquez-Ruiz G Dobarganes C 《Journal of agricultural and food chemistry》2005,53(10):4006-4011
Major short-chain glycerol-bound compounds were investigated in olive oil (OO) and conventional sunflower oil (SO) during thermoxidation at 180 degrees C for 5, 10, and 15 h. These compounds included methyl heptanoate (C7:0), methyl octanoate (C8:0), methyl 8-oxo-octanoate (8-oxo-C8:0), methyl 9-oxononanoate (9-oxo-C9:0), dimethyl octanodiate (C8:0 diester), and dimethyl nonanodiate (C9:0 diester), which were analyzed by GC after derivatization of triacylglycerols to fatty acid methyl esters. An acceptable linear correlation (r = 0.967) was found between the total content of these compounds and the total content of polar compounds, suggesting that quantitation of the major short-chain glycerol-bound compounds provides a good indication of the total alteration level of oils heated at frying temperature. Samples with levels of polar compounds around 25% on oil showed total contents within 2-3 mg/g of oil. To determine the content of these compounds in used frying oils, 10 samples from restaurants and fried-food outlets in Spain were analyzed. Results showed total levels between 2.13 and 7.56 mg/g of oil in samples with contents of polar compounds ranging from 18.8 to 55.5% on oil. Samples with levels of polar compounds of approximately 25% showed total contents of the short-chain compounds similar to those found in the thermoxidized oils, that is, within 2-3 mg/g of oil. 相似文献
964.
Rodríguez R Jaramillo S Rodríguez G Espejo JA Guillén R Fernández-Bolaños J Heredia A Jiménez A 《Journal of agricultural and food chemistry》2005,53(13):5212-5217
Three different methods (antiradical activity, inhibition of primary oxidation, and ferric reducing power) have been used to evaluate the antioxidant activity of eight different asparagus cultivars and byproducts: white and green asparagus from Alcalá del Río (Guadalquivir Valley, Seville) and American hybrids, native spears, and their byproducts from Huétor-Tájar (Vega de Granada). The correlation between antioxidant activity and total phenol content was studied. Six standards were also tested to validate the modified methods for antioxidant activity determination. Results obtained for antiradical capacity and reducing power were very similar, and a high correlation with phenols was found (R > or = 0.9 for both tests). Sample origin was an important factor, spears from Huétor-Tájar having higher values (ARC between 7 and 10 and P(R) of 0.25-0.33) than those from Alcalá del Río (ARC 0.6-2 and P(R) of 0.05-0.07). Significant differences were found between spears with the same origin, suggesting that genetics are another factor to take into account. Asparagus inhibits lipid primary oxidation, but no correlation between the inhibition percentage and phenols was observed. Asparagus origin was the only factor that led to significant differences: samples from Huétor-Tájar had higher values (POIC between 18 and 32) than those from Alcalá del Río (POIC of 5-9). Byproducts from the canning industry at Huétor-Tájar were also assayed for antioxidant activity; the results obtained suggested that byproducts could be considered as an excellent source of natural antioxidants. 相似文献
965.
Rodríguez García JC Barciela García J Herrero Latorre C García Martín S Peña Crecente RM 《Journal of agricultural and food chemistry》2005,53(17):6616-6623
In the present work, direct methods for the determination of chromium, copper, and nickel in honey by electrothermal atomic absorption spectroscopy were developed using experimental design as an optimization tool. Once the optimum conditions for the individual methods were established, a direct method for the combined determination of the three elements was optimized using the response surface tool. Palladium was used as chemical modifier in all cases. Honey was diluted in water, hydrogen peroxide, and nitric acid. Triton X-100 was added to minimize the matrix effect and the viscosity of the sample. The RSD (better than 10%) and the analytical recovery (98-103%) were acceptable for all of the developed methods. Calibration graphs were used in the four methods to determine the concentration of the analytes in the sample. The detection limits of the combined method (0.21, 0.35, and 0.37 microg L(-)(1) for Cr, Cu, and Ni, respectively) were similar to those obtained for the individual methods (LOD = 0.17, 0.21, 0.33 microg L(-)(1) for Cr, Cu, and Ni, respectively). The direct-combined proposed method has been applied to the determination of chromium, copper, and nickel content in representative honey samples from Galicia (northwestern Spain). The concentrations found in the analyzed samples were in the range of (5.75 +/- 0.64)-(26.4 +/- 0.38) ng g(-)(1) of Cr, (79 +/- 7.8)-(2049 +/- 80) ng g(-)(1) of Cu, and (12.6 +/- 1.36)-(172 +/- 6.88) ng g(-)(1) of Ni. 相似文献
966.
Participatory landrace selection for on-farm conservation: An example from the Central Valleys of Oaxaca, Mexico 总被引:8,自引:1,他引:8
Mauricio R. Bellon Julien Berthaud Melinda Smale José Alfonso Aguirre Suketoshi Taba Flavio Aragón Jaime Díaz Humberto Castro 《Genetic Resources and Crop Evolution》2003,50(4):401-416
On-farm conservation is recognized as a key component of a comprehensive strategy to conserve crop genetic resources. A fundamental problem faced by any on-farm conservation project is the identification of crop populations on which efforts should be focused. This paper describes a method to identify a subset of landraces for further conservation efforts from a larger collection representing the diversity found in the Central Valleys of Oaxaca, Mexico. Mexico is a center of origin and diversity for maize (Zea mays L.). The 17 landraces selected from an initial collection of 152 satisfy two criteria. First, they represent the diversity present in the larger collection. Second, they appear to serve the interests of farmers in the region. Data for applying the method were elicited through participatory as well as conventional techniques. They incorporate the complementary perspectives of both men and women members of farm households, and of plant breeders and social scientists. 相似文献
967.
Martín-Cabrejas MA Ariza N Esteban R Mollá E Waldron K López-Andréu FJ 《Journal of agricultural and food chemistry》2003,51(5):1254-1259
The effect of different conditions of pea germination on dietary fiber (DF) composition was studied. Insoluble dietary fiber (IDF) and soluble dietary fiber (SDF) were subjected to acid hydrolysis, and the resultant neutral sugars, uronic acids, and Klason lignin were quantified. Germinated peas exhibited significantly higher contents of total dietary fiber (TDF) than the raw sample, due to the increases of both DF fractions. Under darkness conditions, germination exhibited the highest contents of IDF and SDF. Decreasing IDF/SDF ratios showed that the carbohydrate changes did not take place to the same extent during germination, the SDF fraction being the most affected. The detailed chemical composition of fiber fractions reveals increases of cellulose in the IDF of germinated samples, whereas SDF exhibits a decrease of pectic polysaccharides and also increases of polysaccharides rich in glucose and mannose. The DF results were corroborated by a comparative examination of the cell wall carbohydrate composition. 相似文献
968.
Gallardo-Guerrero L Hornero-Méndez D Mínguez-Mosquera MI 《Journal of agricultural and food chemistry》2002,50(23):6746-6751
The pectic and pigment compositions and Ca and Cu contents of the alcohol-insoluble solid (AIS) residues were determined in cv. Gordal olives treated with NaOH solution and kept at different constant pH values (3.5-6.5). The same controls were made in table olives presenting green staining alteration. The ratio between the various pectin fractions of the more acid pH experiment samples remained similar in fruits not showing green staining. In altered fruits, the protopectin fraction was lower, and the calcium pectate or EDTA soluble pectins were higher. Regarding the presence of Ca and Cu in the AIS, it was observed that, whereas Ca levels fell at the most acid pH values, those of Cu increased. The concentration of Ca was higher in the AIS of altered olives than in nonaltered ones. The same trend was seen for the zone with or without green staining of an altered fruit. In the case of Cu, the relationship was the opposite: a decrease in the levels of AIS Cu in fruits and zones of fruits with green staining. This result was correlated with the highest concentration of Cu-chlorophyll complexes found in such samples and suggested that pectins might act as a reservoir of Cu involved in the alteration. 相似文献
969.
Duarte-Vázquez MA García-Almendárez BE Regalado C Whitaker JR 《Journal of agricultural and food chemistry》2001,49(9):4450-4456
A neutral peroxidase isozyme (pI 7.2) from turnip roots (TNP) was purified to homogeneity and partially characterized. TNP is a monomeric glycoprotein with 9.1% carbohydrate content and a molecular weight of 36 kDa. Optimum pH values for activity using 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) and guaiacol as H donors were 4.5 and 5.5, whereas the K(m) values were 0.7 and 3.7 mM, respectively. The ABTS K(m) was approximately 7 times higher than that reported for basic commercial horseradish peroxidase (HRP-C). TNP retained approximately 70% activity after 11 min of heating at 65 degrees C, whereas the activation energy for inactivation (132 kJ/mol) was higher than or comparable to that of other peroxidases. The low ABTS K(m) and high specific activity (1930 units/mg) gave a high catalytic efficiency (500 M(-1) s(-1)). These properties make TNP an enzyme with a high potential as an alternative to HRP in various applications. 相似文献
970.
Katia Duquesne Aurlie Lieutaud Jeanine Ratouchniak Andrs Yarzbal Violaine Bonnefoy 《European Journal of Soil Biology》2007,43(5-6):351
Weathering of the arsenopyrite-rich tailings of the Carnoulès abandoned mining site (Gard, France) results in the formation of acid mine waters heavily loaded with arsenic. Dissolved arsenic present in the seepage waters is precipitated within a few meters from the bottom of the tailing dam by microorganisms. From these effluents, we have isolated and characterized a Gram negative bacterium involved in this bioremediation process. This bacterium, which belongs to the Thiomonas genus, is very efficient in removing arsenite from contaminated acidic waters by oxidizing it to arsenate, which precipitates. 相似文献