全文获取类型
收费全文 | 3712篇 |
免费 | 234篇 |
国内免费 | 242篇 |
专业分类
林业 | 335篇 |
农学 | 335篇 |
基础科学 | 182篇 |
591篇 | |
综合类 | 933篇 |
农作物 | 263篇 |
水产渔业 | 267篇 |
畜牧兽医 | 899篇 |
园艺 | 125篇 |
植物保护 | 258篇 |
出版年
2024年 | 16篇 |
2023年 | 52篇 |
2022年 | 112篇 |
2021年 | 158篇 |
2020年 | 150篇 |
2019年 | 135篇 |
2018年 | 128篇 |
2017年 | 172篇 |
2016年 | 117篇 |
2015年 | 144篇 |
2014年 | 169篇 |
2013年 | 227篇 |
2012年 | 240篇 |
2011年 | 239篇 |
2010年 | 207篇 |
2009年 | 177篇 |
2008年 | 230篇 |
2007年 | 205篇 |
2006年 | 170篇 |
2005年 | 151篇 |
2004年 | 96篇 |
2003年 | 66篇 |
2002年 | 87篇 |
2001年 | 83篇 |
2000年 | 83篇 |
1999年 | 80篇 |
1998年 | 34篇 |
1997年 | 52篇 |
1996年 | 40篇 |
1995年 | 49篇 |
1994年 | 25篇 |
1993年 | 42篇 |
1992年 | 22篇 |
1991年 | 26篇 |
1990年 | 19篇 |
1989年 | 19篇 |
1988年 | 18篇 |
1987年 | 14篇 |
1986年 | 14篇 |
1984年 | 8篇 |
1983年 | 7篇 |
1982年 | 8篇 |
1980年 | 5篇 |
1979年 | 8篇 |
1978年 | 7篇 |
1976年 | 6篇 |
1970年 | 5篇 |
1969年 | 5篇 |
1968年 | 6篇 |
1967年 | 8篇 |
排序方式: 共有4188条查询结果,搜索用时 421 毫秒
121.
122.
123.
124.
125.
126.
Thi Thanh Nhan Pham Thi Ngoc Lan Nguyen Thi Ha Bui Huu Quan Nguyen Thi Tam Nguyen Van Son Le 《The Journal of Horticultural Science and Biotechnology》2019,94(5):591-598
Catharanthus roseus contains vincristine and vinblastine, which are outstanding drugs for cancer. In the biosynthetic pathways of terpenoid indole alkaloids (TIAs) in C. roseus, deacetylvindoline 4-O-acetyltransferase (DAT) is a key enzyme that catalyses the last reaction of vindoline biosynthesis to form vinblastine and vincristine. In this study, the CrDAT transgene was transferred into the periwinkle by Agrobacterium-mediated transformation and generated transgenic periwinkle lines with an increase in vincristine accumulation. The C. roseus DAT gene was introduced into C. roseus plants and it was confirmed that CrDAT was successfully transferred into the genome of periwinkle plants and efficiently translated to synthesise recombinant DAT protein. Four transgenic periwinkle lines in T1 generation, T1-1, T1-3, T1-6, and T1-7, expressed recombinant DAT protein with the total protein content in the range of 2.86 μg.mg?1 to 5.12 μg.mg?1. Moreover, the vincristine contents of four transgenic lines increased by 1.63?2.48-fold compared to non-transgenic plants, ranging from 6.91 µg.g?1 (fresh weight) to 10.53 µg.g?1 (fresh weight). The T1-1 line had the highest vincristine content. Hence, the overexpression of the recombinant DAT protein can improve the vincristine accumulation of transgenic C. roseus plants.Abbreviation: CrDAT - Catharanthus roseus Deacetylvindoline-4-O-Acetyl Transferase; D4H - Deacetoxyvindoline 4-hydroxylase; ELISA - Enzyme-Linked Immunosorbent Assay Monoterpene indole alkaloid; T0, T1 - Generations of transgenic plants; TIAs - Terpenoid indole alkaloids; WT- The wild-type tobacco plants (non transgenic plant); 35S - Cauliflower mosaic virus 35S promoter 相似文献
127.
Climate change has become a global issue influencing human survival and development while low‐carbon is the inevitable choice. As the world’s largest emitter of greenhouse gases, China has become an important force that influences the cooperation of climate change. China is a large agricultural country, and the rural carbon emissions have been gradually increasing. We consider rural neighborhood as an important space for the use of low‐carbon ideas to address climate change. There is no specific assessment system for a rural neighborhood. The studies of rural neighborhood low‐carbon mainly focus on the method of calculation carbon emissions. However, only quantifying rural carbon emissions is insufficient. In this paper, a low‐carbon evaluation indicator system has been proposed for rural neighborhood and Fuzzy Comprehensive Evaluation has been applied to get the low‐carbon degree. Considering the influence and feedback inside the indicator system, Analytic Network Process was applied to get the weights. Furthermore, a case study was carried out for the using of the proposed method in an eco‐village of China. Our practice has proved that the system is easy to operate in rural neighborhood. 相似文献
128.
Marine Ollier Vincent Talle Anne‐Laure Brisset Zo Le Bihan Simon Duerr Marc Lemmens Ellen Goudemand Olivier Robert Jean‐Louis Hilbert Hermann Buerstmayr 《Plant Breeding》2019,138(1):69-81
Fusarium head blight (FHB) is a cereal disease of major importance responsible for yield losses and mycotoxin contaminations in grains. Here, we introduce a new measurement approach to quantify FHB severity on grains based on the evaluation of the whitened kernel surface (WKS) using digital image analysis. The applicability of WKS was assessed on two bread wheat and one triticale grain sample sets (265 samples). Pearson correlation coefficients between Fusarium‐damaged kernels (FDK) and WKS range from r = 0.77 to r = 0.81 and from r = 0.61 to r = 0.86 for the correlation between deoxynivalenol (DON) content and WKS. This new scoring method facilitates fast and reliable assessment of the resistance to kernel infection and shows significant correlation with mycotoxin content. WKS can be automated and does not suffer from the “human factor” inherent to visual scorings. As a low‐cost and fast approach, this method appears particularly attractive for breeding and genetic analysis of FHB resistance where typically large numbers of experimental lines need to be evaluated, and for which WKS is suggested as an alternative to visual FDK scorings. 相似文献
129.
红肉猕猴桃中果皮和内果皮在果实发育不同时期色泽变化模式有显著差异,但目前对于其内在的分子调控机制尚不清楚。本研究中,利用高通量测序技术,对红肉猕猴桃(‘红实2号’)中果皮与内果皮4个不同发育时期(开花后0 d, 28 d, 75 d, 157 d,分别标注为T1时期, T2时期, T3时期, T4时期)的样本进行转录组测序。经去除低质量数据后,得到Clean reads数目为2 192~4 331万,检测到的基因数目为24 607~29 170个。在(log2 Ratio)≥1和FDR<0.05阈值下,中果皮和内果皮在T2、T3、T4时期检测到的差异基因数目分别为1 880个、4 799个、7 346个和4 31个、1 493个、7 700个。KEGG分析显示这些差异表达基因被富集到68个代谢途径,包括DNA复制、RNA转运、蛋白质输出、植物激素信号转导、类黄酮生物合成、花青素合成、淀粉与蔗糖的代谢等。此外我们还鉴定到13个花青素合成相关基因差异表达,大部分基因的表达量随果实的发育呈现下降趋势,这一结果与花青素含量变化模式相吻合。本研究不仅深化了对猕猴桃果实成色差异机理的认识,还对今后的彩色猕猴桃选育种工作有一定的帮助。 相似文献
130.
为了揭示陆地棉生长调控因子(growth-regulating factors, GRF)家族的功能,本研究利用全基因组信息在陆地棉基因组中鉴定了 33个GRF蛋白,并对这些家族成员进行了保守结构域分析、蛋白的基本理化性质分析、进化分析、基因的结构分析、染色体定位、保守基序分析和组织表达模式分析。研究表明,陆地棉GRF均含有QLQ和WRC 2个保守结构域,系统进化分为4个亚家族,基因定位在19条染色体上,基因上有2~19个蛋白编码区域,大多数的基因在柱头、子房和20 d的种子中高量表达。本研究弄清了棉花GRF家族的基本信息,为进一步研究棉花GRF家族成员的功能提供了理论指导。 相似文献