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111.
J Tetens S A Barker M Waguespack G Hosgood 《Canadian journal of veterinary research》2001,65(2):133-135
The objectives were to use high performance liquid chromatography (HPLC) to validate an established method for adenine nucleotide separation in equine colonic mucosal tissue, to determine the inherent variability in the tissue and extraction method, and to determine the stability of ATP, ADP, and AMP in the tissue with time. Equine colonic mucosal tissue obtained from a single horse was immediately submersed in liquid nitrogen, and stored at -70 degrees C. Samples were lyophilized, extracted, and separated by HPLC. The limit of quantitation was 0.05 microg/mL. The coefficient of variation for the instrument was less than 10% for all nucleotides measured. When the tissue was not homogenized prior to sampling, there were significant differences in adenine nucleotide content between samples. However, when the tissue was homogenized prior to analysis, these differences were no longer significant. There was no significant decrease in ATP, ADP, or AMP content over a 54-day analysis period. 相似文献
112.
Transmission of porcine reproductive and respiratory syndrome virus from persistently infected sows to contact controls. 总被引:3,自引:0,他引:3 下载免费PDF全文
M D Bierk S A Dee K D Rossow S Otake J E Collins T W Molitor 《Canadian journal of veterinary research》2001,65(4):261-266
The objective of this study was to determine if porcine reproductive and respiratory syndrome virus (PRRSV) could persist in non-pregnant sows and if persistently infected sows could transmit virus to naive contact controls. Twelve PRRSV-naive, non-pregnant sows (index sows) were infected with a field isolate of PRRSV and housed in individual isolation rooms for 42 to 56 days postinfection. Following this period, 1 naive contact sow was placed in each room divided by a gate allowing nose-to-nose contact with a single index sow. Index sows were not viremic at the time of contact sow entry. Virus nucleic acid was detected by polymerase chain reaction, and infectious virus was detected by virus isolation in sera from 3 of the 12 contact sows at 49, 56, and 86 days postinfection. All 3 infected contacts developed PRRSV antibodies. Virus nucleic acid was detected in tissues of all of the 12 index sows at 72 or 86 days postinfection. Nucleic acid sequencing indicated that representative samples from index and infected contacts were homologous (> 99%) to the PRRSV used to infect index sows at the onset of the study. This study demonstrates that PRRSV can persist in sows and that persistently infected sows can transmit virus to naive contact animals. 相似文献
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目前农业信息化发展面临的问题与几点建议 总被引:3,自引:1,他引:2
本文探讨了农业信息服务特点及开展与推广所面临的主要问题,对完善农业信息服务、实现农业信息化提出相关建议。 相似文献
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造林新材料在岱山海岛造林中的应用 总被引:3,自引:0,他引:3
通过3种造林新材料在岱山海岛造林中的应用,观测它们对提高造林成活率的影响.结果表明:3种造林新材料对提高夹竹桃Nerium indicum造林成活率基本没有影响,科瀚98对枫香Liquidambar formosana和银杏Ginkgo biloba造林成活率影响较小.植树宝、科瀚98和GGR可显著提高女贞Ligustrum lucidum,重阳木Bischofia javanica,香樟Cinnamomum camphora和木荷Schima superba的造林成活率.图1表1参6 相似文献
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T D Butt A M Cruz J V Bailey W H Crawford 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(12):936-939
The object of this investigation was to determine the outcome of limb amputation in wapiti. Medical records of 13 wapiti that underwent limb amputation were reviewed to determine age, weight, sex, injury preceding amputation, limb amputated, amputation location, length of hospital stay, complications, and outcome. Ages ranged from 3 months to 8 years and weights from 70 kg to 280 kg. All animals were female. Eleven animals sustained catastrophic long bone fractures prior to amputation. Five animals had front limb amputations and 8 had hind limb amputations. Of these animals, 1 with a front limb amputaiton and 5 with hind limb amputations survived. Postsurgical inability to stand is associated with hospitalization of > 1 day. Of the 13 wapiti reviewed, 5 with hind limb amputations have produced and reared offspring. Hind limb amputation can be considered a viable alternative to euthanasia when catastrophic orthopedic injuries occur in female wapiti. 相似文献
120.
当压缩机内气体的流量较低时,气体容易出现旋转脱离现象,导致压缩机不能正常工作,采取增加压缩机内部液体的流量,在出口处设置单向阀防止气体倒流等措施,可有效地防止喘振的发生,保证输气管道的正常运行. 相似文献