Effect of scutellarin on resuscitating rat NHBMD livers with extracorporeal liver perfusion in vitro

AIM: To investigate the effect of scutellarin on resuscitating rat non-heart-beating marginal donor (NHBMD) livers with extracorporeal liver perfusion in vitro. METHODS: Twenty-four male Wistar rats were randomly divided into 3 groups: group A (n=8), the livers were subject to 15~20 min of warm ischemia; group B (n=8) and group C (n=8), the livers were subject to 45 min of warm ischemia. All the livers were treated by extracorporeal liver perfusion (ECLP), and scutellarin solution was add to the perfusion fluid in group C with the intervals of 1 h. The data of bile production and the markers of hepatocyte reperfusion injury of the livers,alanine aminotransferase (ALT) and endothelin-1 (ET-1), in each group were tested. The liver tissues in each group were obtained before perfusion and 1 h, 3 h and 6 h after perfusion, and then observed under transmission electron microscope to investigate the changes of ultrastructures. RESULTS: With the time of perfusion went on, the livers in group B and group C demonstrated recovery of synthe-tic function after 3 h and 6 h of perfusion, and the production of bile in group B and group C was not significantly different from that in group A after 6 h of perfusion (P>0.05). The levels of ALT and ET-1 in group B and group C were significantly higher than those in group A after 1 h, 3 h and 6 h of perfusion (P<0.05), and those in group C was significantly lower than those in group B after 3 h and 6 h of perfusion (P<0.05). The ultrastructural destruction of the liver cells in both group B and group C was more serious than that in group A. After 3 h and 6 h of perfusion, the destruction of the liver cells was partly recovered, and the recovery degree in group C was more obvious than that in group B. CONCLUSION: Scutellarin may play a significant role in resuscitating rat NHBMD livers with extracorporeal liver perfusion in vitro.     

染色体研究的新方法与新进展

从染色体显微分离与显微克隆、染色体连锁图谱、染色体原位杂交、染色体涂染、染色体的基因定位以及染色体的片段转移等几个方面,分别综述了染色体研究的新方法和新进展,并展示了分子细胞遗传学的发展前景。     

赤霉素对洋桔梗幼苗生长影响的研究

采用叶面喷施将不同浓度的赤霉素(GA)喷于洋桔梗幼苗上,分别在全日照、强光,25℃和弱光、常温(15～1 7℃)条件下进行培养,观察洋桔梗幼苗的生长动态,测量株高、茎粗、叶片大小及其根冠比。结果表明,在全日照、强光,25℃条件下,洋桔梗幼苗生长较好,且在浓度为200mg/L和400mg/L赤霉素处理下,株高、叶片大小及其根冠比等形态指标均显著高于对照,达到极显著水平,在600mg/L和800mg/L赤霉素处理下无明显变化。     

百合鳞茎解除休眠与花期调控的研究

探讨了亚洲百合杂种系(Asiatic hybrids)‘新中心(Nove Cento)’在12℃冷藏过程中冷处理时间长短与花芽分化、花期调控之间的关系。结果表明:从冷藏开始至60d,冷藏时间越长,鳞茎解除休眠所需时间越短,冷藏45d以上,可完全解除鳞茎休眠;百合的花芽分化从冷藏45d开始,栽植后30d基本完成;冷处理时间越长,百合植株开花越早,花茎越矮,而且还会出现二次抽薹开花的现象。     

铁皮石斛无土栽培基质的研制与优化

为筛选铁皮石斛组培苗的无土栽培基质,在温室内采用细锯末、苔藓、蛭石、松树皮配制成10种不同的栽培基质,栽培60 d后统计成活率;120 d后测定铁皮石斛苗在各基质中的生长指标。结果表明：铁皮石斛组培苗在细锯末∶松树皮∶苔藓=2∶4∶4的基质中成活率最高,移栽60 d时成活率达93.30%,植株高度达2.85 cm;移栽120 d后试管苗在细锯末∶松树皮∶苔藓=2∶4∶4的基质中平均株高12.49 cm、发根数量为8条、最长根系为3.49 cm,平均茎粗为0.93 cm、平均萌芽数为3个,且叶色浓绿、生长健壮,总体情况显著优于其他基质配比,为当地铁皮石斛组培苗栽培松树皮、锯末等废弃物的利用提供参考。     

同源四倍体水稻三系亲本主要农艺性状遗传改良

从1996年至2006年对同源四倍体水稻亲本材料的结实率、每穗实粒数、穗长和有效分蘖等主要农艺性状进行了连续地选择和改良.结实率的改良效果显著,所有材料的平均结实率均呈上升趋势.每穗实粒数的改良上升趋势比较显著,除保持系的DTB之外,其余材料的每穗实粒数有显著提高.穗长和有效分蘖总体上有所提高,但不同年份有较大的波动.对株高、剑叶长等性状的选育效果不显著.研究表明:不同品系间农艺性状差异显著高于品系内,说明遗传力较显著地决定重要农艺性状,通过连续改良,重要农艺性状可以得到显著改善.该结果为同源四倍体水稻的进一步改良提供了一定的理论支持.

Abstract：

The autotetraploid rice three-line materials were created by using colchicine to induce diploid rice and, after that, successive selection and improvement on seed setting, total grains per panicle, panicle length, productive tillers per plant and some other traits were carried out from 1996 to 2007. The improvement of average seed setting was significant in all materials. Total grains per panicle increased significantly in all strains studied except DTB. On the whole, panicle length and the productive tillers per plant were improved, though fluctuations were recorded between different years. Other agronomic traits, including plant height and flag leaf length, were improved by selection, but not significantly. These results suggested that the major agronomic traits are mainly affected by heredity and that agronomic traits could be improved by successive selection.     

2011年江汉平原高温干旱天气对油菜千粒重的影响

2011年3～5月间江汉平原遭遇罕见的高温干旱天气。采用大范围多点田间取样和气候条件比对分析方法,探讨了该天气对江汉平原油菜(Brassica napus L.)千粒重的影响。结果表明,2011年4月下旬至5月中旬的日平均气温与前50年同期日平均气温相比提高3.8℃,4月上旬至5月上旬降雨量平均降低16.2 mm,4月中旬至5月上旬空气相对湿度平均降低5.7个百分点。在此条件下,江汉平原油菜千粒重普遍降低,降低田块数占被调查田块数的62%左右,水田和旱田千粒重分别降低0.527 g和0.390 g。千粒重降低的原因主要与油菜灌浆结实期间的气温异常升高有关,而与土壤水分关系不密切,但不能排除空气湿度明显降低的负面影响。     

威宁地八角与多年生黑麦草混播草地的生物量及种间竞争动态

为了给威宁地八角在人工混播草地中的推广应用价值提供参考,以威宁地八角和尼普顿多年生黑麦草为材料,采用混播方式研究其群落的生物量、株高及种间竞争力变化。结果表明:75%的多年生黑麦草+25%地八角为最佳混播组合;从混播生长群落看,多年生黑麦草对地八角可起扶持作用,使其不倒伏,利于刈割,而地八角的固氮效果对多年生黑麦草的生长也有促进作用,两者具有较好的共生关系,竞争力相似。     

滇东东紫苏资源的调查与评价

采用踏查、详查和访问调查的方法,对云南东部的东紫苏资源进行了资源调查,在此基础上评价了该种植物的资源动态和现状,为其深入研究提供了参考.调查结果表明:在所调查的地区中,只有楚雄市和武定县有极丰富的东紫苏资源分布；其余各地东紫苏资源均不容乐观,尤其是大理和弥勒的东紫苏野生资源破坏极为严重.因此认为云南东部的东紫苏植物资源分布带狭窄,生境要求严格；各地资源破坏严重,贮量正在缩减.     

云南高原特色农产品的目标营销战略

随着云南省第九次党代会做出了发展高原特色农业的战略部署,在云南省范围内,各地针对本区域地理、气候、物种等优势和特点,积极投身于高原特色农业的建设中。但是如何把优质的高原特色农产品推入市场,卖到好价格,实现其高价值,关乎高原特色农业的持续发展。本文认为采用目标市场营销战略的方法可以帮助优质产品实现其价值,促进农民增收。