Hydrogen sulfide ameliorates high glucose-induced endothelial cell senescence by suppressing oxidative stress

AIM：To explore the effect of hydrogen sulfide on the senescence of human umbilical vein endothelial cells (HUVECs) induced by high glucose. METHODS：Senescence model was established by treating HUVECs with 33 mmol/L glucose for 48 h. The parameters were detected to demonstrate the effect of hydrogen sulfide on senescence and the mechanism involved was also investigated. RESULTS：In the cells treated with high glucose, the proliferation was attenuated with a higher number of senescence-associated β-galactosidase (SA-β-Gal) positive cells, and plasminogen activator inhibitor 1 (PAI-1) protein expression, malondialdehyde (MDA) production and NF-κB p65 activity were increased significantly, but the expression of superoxide dismutase 1 (SOD1) was decreased. However, the cell number and SOD1 expression were increased, and the number of SA-β-Gal positive cells, PAI-1 protein expression, MDA production and the activity of NF-κB p65 were decreased after sodium hydrosulfide (100 and 200 μmol/L) treatment.CONCLUSION：Exo-genous hydrogen sulfide prevents HUVECs against high glucose-induced senescence by suppressing oxidative stress and NF-κB p65 activity.     

New Concepts for Administration of Construction Quality in China in the 21st Century

The 21st century is a century with focus on quality. The concept of construction engineering quality will greatly extended. The theory and method of construction quality administration will have great advancement in the 21st century. It will be shown in many respects, such as TQ, QR, CS, IC and QE. After the more and more enterprises tend to use ISO, it will naturally result in the general trend that three ISO systems will be combined into one.     

思茅松良种利用方法的探讨

思茅松良种利用,根本是对思茅松优良性状资源的利用。思茅松资源收集是进行性状改良的前提;通过有性改良可获得优良性状;无性是对优良性状的直接利用。比较各类无性繁殖方法,目前,思茅松良种利用最有效的方式是通过嫁接方式建立采穗圃,生产幼化穗条,扦插利用。     

吡唑醚菌酯对两种优稀水果病原菌的毒力测定及田间防治效果

为推荐登记优稀水果重要病害的防治用药,有效缓解小宗作物病害防治缺药或无药可用的现状。采用室内毒力测定及田间药效试验,评价30%吡唑醚菌酯悬浮剂防治黄皮炭疽病和番石榴叶斑病的防治效果及安全性。试验结果表明,吡唑醚菌酯对黄皮炭疽病菌和番石榴叶斑病菌菌丝生长均有较好的抑制作用,EC₅₀分别为 0.0321 μg/mL 和 2.0167 μg/mL,均显著优于对照药剂嘧菌酯的抑菌效果;30%吡唑醚菌酯悬浮剂有效成分用量 250 mg/kg 对黄皮炭疽病和番石榴叶斑病具有较好的田间防治效果,施药处理3次后对2种果树病害的防治效果分别达到78.68%和77.45%,显著优于对照药剂25%吡唑醚菌酯悬浮剂200 mg/kg的处理。30%吡唑醚菌酯悬浮剂对黄皮炭疽病和番石榴叶斑病均具有较好的防治效果,值得应用于两种果树病害的防治与示范推广。     

普通小麦品种小偃54中α/β-醇溶蛋白编码基因的克隆与序列分析

醇溶蛋白是小麦籽粒贮藏蛋白的重要组分,其组成与含量对小麦加工品质具有重要影响。本研究建立了利用PCR从普通小麦基因组BAC文库中筛选含有α/β-醇溶蛋白基因序列BAC克隆的方法,并获得9个不同的含有α/β-醇溶蛋白基因的BAC克隆。从其中鉴定出17个α/β-醇溶蛋白基因,其编码区序列长度为852~957 bp。12个序列在编码区内存在提前终止密码子,推测为假基因。其他5个成员(Gli-Xy54-1、Gli-Xy54-2、Gli-Xy54-3、Gli-Xy54-7和Gli-Xy54-13)分别编码291、310、311、287和317个氨基酸残基,都具有α/β-醇溶蛋白一级结构的典型特征。根据推导的氨基酸序列中乳糜泻病诱发因子的分布情况及多聚谷氨酰胺重复区的长度差异,推测Gli-Xy54-7可能定位于6A染色体,Gli-Xy54-2、Gli-Xy54-3和Gli-Xy54-13可能定位于6B染色体,Gli-Xy54-1可能定位于6D染色体。基因聚类分析支持了上述推论。这是第一次从普通小麦中筛选到包含α/β-醇溶蛋白基因的BAC克隆,并从中得到目标基因全长,对进一步研究普通小麦基因组中α/β-醇溶蛋白编码基因的组成、表达与功能有较好的参考价值。     

玉米D亚族bZIP转录因子基因的数据库挖掘、分析、克隆与表达

碱性亮氨酸拉链(basic leucine zipper, bZIP)是真核生物特有的转录因子家族,在高等植物基因表达与调控中起重要作用。本文通过对玉米基因组数据库中收录的全长cDNA序列全基因组范围的bZIP分析,发现其中25个序列编码D亚族bZIP转录因子。针对这些序列进行生物信息学分析、染色体分布和直系同源组的分类分析,发现部分序列可能是由同一基因座编码,但不同方式剪切形成的。对部分基因克隆和测序发现了更多的剪切形式。定量检测其中3个基因在ABA和干旱胁迫条件下的表达发现,其中1个受ABA诱导,2个受ABA抑制,但均不受干旱胁迫影响。表明玉米中D亚族基因可能参与ABA响应。     

Development of integrated reactors for domestic garbage and town sludge

Aiming at domestic garbage aerobic compost and town sludge anaerobic treatment, on the basis of reactor TISTD (the integrated of sludge thickening and digestion)which is developed by the authors’ team, a TIDGTS (the integrated of domestic garbage and town sludge)reactor is designed.The reactor is made up of garbage and sludge chambers.Aerobic compost is adopted in the garbage chamber, while anaerobic fermentation is used in the sludge chamber, thus garbage and sludge can be treated in the same reactor.32~36 ℃ of sludge digestion can be ensured by the heat from sludge digestion, and 20 L sludge and 15 kg garbage can be treated per day.While inlet sludge moisture content is 97.2%~99.8% and VS/TS is 0.45~0.69, the discharged sludge moisture content is 92.7%~96.1% and VS/TS is 0.28~0.42.When the inlet garbage moisture content is 73.5% and VS/TS is 0.65~0.88, the discharged garbage moisture content is 48.6% and VS/TS is 0.32~0.44.The results show that the efficiency of concentration and digestion is near to TISTD which is heated by heating wire.     

Microscopical static properties of the confined fluids in nanometer tubes

The static properties of the confined in the nanometer cylinder tubes are investigated by molecular dynamics simulation. The modified Lennard Jones potential function is adopted to describe the interaction between fluid fluid and fluid solid. In which, the introduced parameter α can be adjusted to change the wetting intensity between the fluids and solid solid wall. Furthermore, the microscopic properties of the fluids in the nanometer tube are characterized by the density distribution, the pair distribution function and the axial velocity distribution. The results show that the interaction strength of the atoms between the fluids and solid wall has a significant influence on the microscopic properties of the fluids as well changes the microscopic state in the different radial region and the motional properties of the atoms consisted of the fluids in the tube.     

思茅松松香歧化反应活性

通过对思茅松松香理化指标和成分进行分析,与马尾松松香对比,探讨其岐化反应活性。结果表明,思茅松松香异海松酸含量为3.57%,高于马尾松松香;歧化反应后产品质量达到LY/T 1357-2008标准的要求,不同厂家生产的思茅松松香歧化的效果差异较大,脱氢枞酸含量在52.4%~73.5%。     

基于ALOS PALSAR数据的森林蓄积量估测技术研究

以吉林省汪清林业局为研究区,基于ALOS PALSAR和森林资源二类清查固定样地数据,利用非线性回归方法建立了固定样地蓄积量与所对应的PALSAR像元后向散射系数之间的关系,结果表明,除杨树(Populus us-suriensis)等树种组外,PALSAR的HV后向散射系数与蓄积量呈良好的正相关关系,对多数树种而言,交叉极化方式(HV)后向散射系数与蓄积量的决定系数比同极化方式(HH)的略高。若以林场为单位统计,采用回归方法得到的估测结果与直接利用固定样地估测的结果相差很小。