在常压和加压条件下直接合成萜烯马来酸二甲酯加合物

以工业双戊烯为原料,马来酸二甲酯为双烯加成试剂,在常压和加压两种条件下合成了萜烯马来酸二甲酯加合物。常压下,加热至185℃回流10 h,产率为55%;压力反应釜中,压力为1.5 MPa,反应温度为240℃,反应2 h即可进行完全,产率为78.6%。纯化产物用GC-MS、1H NMR和FT-IR进行了表征。GC-MS表明经蒸馏纯化的产物中分子离子峰质荷比为280.1的萜烯马来酸二甲酯同分异构体约有20个,纯度为95.2%。经产物的1H NMR计算得到产物中Diels-Alder加成物质量分数为35%。     

培养条件对仙鹤草愈伤组织诱导、增殖及总黄酮含量的影响

以仙鹤草(Agrimonia pilosa Ledeb)无菌苗的子叶为外植体,采用正交设计实验法,研究了不同培养条件对仙鹤草愈伤组织诱导、增殖及总黄酮含量的影响。结果显示:以1/2MS+6-BA 1.0(mg/L)+NAA 0.5(mg/L),在pH 6.5时,愈伤组织诱导率较高,且长势良好;以1/2MS+6-BA 2.0(mg/L)+NAA 1.0(mg/L),在pH 6.0时,愈伤组织增长最快且总黄酮含量最高。     

柴河林业局种苗工程建设的初步设想

为满足柴河林业局林业工程及生态建设的需要,以2006年森林资源二类调查资料为依据,对柴河林业局的林木种苗工作提出了建议。     

呼伦贝尔岭东南旱作丘陵区气候资源潜力分析

对呼伦贝尔市阿荣旗三道沟镇商品粮生产基地主要粮食作物的光合、光温、气候、气候-土壤资源潜力进行分析,结果表明热量条件是限制该地区粮食增产的主要非可控因素,如能改善热量条件,还有20%～30%的增产潜力;该地区的降水条件对玉米、大豆、马铃薯、水稻四种主要粮食作物的满足程度在80%左右,表明降水量及其季节分布是该镇粮食产量低的制约因素之一,存在的问题是水分利用率较低;土壤养分状况对马铃薯和水稻增产未表现明显的限制作用,而对玉米和大豆增产却是最主要限制因素。     

Effects of ibrolipim against atherosclerosis in minipigs

AIM: To explore the application mechanism of NO-1886 (ibrolipim), a synthetic compound, improving dyslipidemia and inhibiting atherosclerosis in Guizhou minipigs fed with high fat/high sucrose diet. METHODS: Fifteen Chinese Guizhou minipigs were randomized into three groups with similar body weight ［(n=5 in normal control group (CD); n=5 in high fat/high sucrose group (HFSD); n=5 in high fat/high sucrose supplemented ibrolipim group （HFSD+ibrolipim)］. Blood samples were withdrawn from the eyehole sinus venosus of the animals at the end of each month after fasting overnight. The animals were sacrificed at the end of 8 months. The concentrations of cholesterol ester in plasma HDL were analyzed by HPLC. The aortic fatty streak-lesions were quantified following lipid staining with Sudan IV. Lipid droplets in liver were observed by Oil red O staining. RESULTS: Compared with CD, fasting plasma TC, TG and FFA levels of HFSD were elevated significantly. The aortic fatty streak-lesions were clearly presented in the animals’ aortas. The intima became rougher and thicker. A lot of lipoid foam cells migrated to regions of intima and smooth muscle cells, which associated with the injuries of internal elastic lamina. Extensive fat deposited in the liver were observed. Supplementing of 1.0% ibrolipim into high fat/high sucrose diet induced the decrease in plasma TG and FFA concentrations and an increase in plasma HDL-C concentration compared with HFSD. A little fat deposited in the liver were observed. CONCLUSION: ibrolipim prevents AS in high fat/high sucrose diet feeding minipigs through decreasing the plasma TG and elevating the plasma HDL-C.     

一次用药与加强护理治疗马属动物破伤风病的研究报告

马属动物破伤风病1次性用药治疗和护理是将破伤风抗毒素30~35万单位、乌洛托品80~100 ml分别混合到500 ml 5％葡萄糖内1次缓慢静注；用药后将患畜用吊马器或绳索，保定在清洁温暖避光安静的厩舍内，使之免受任何不良刺激；畜前摆放水料，精心护理；护理期间用蝉蜕300~400 g分4次煎汤，连饮4 d效果更好。20年的研究结果表明，1次用药采取严格护理治疗的131例全部痊愈。     

畜禽数量性状基因座位的精细定位

数量性状基因座位(QTL)的精细定位是实施QTL克隆及标记辅助选择(MAS)的重要基础。然而就目前畜禽QTL定位的结果来看,除了通过候选基因法识别的少数基因外,大多数QTL定位的精度仍无法满足实际应用的要求。为进一步提高QTL定位的精度,缩小QTL定位的置信区间,人们相继提出并发展了一系列新的QTL定位方法。本文在分析畜禽QTL定位的基本方法及影响畜禽QTL定位精度的主要因素基础上,对提高QTL定位精确性的策略和方法进行了相应的探讨。     

口服ND弱毒苗对鸡十二指肠中2种神经多肽阳性细胞的影响

应用新城疫弱毒苗经消化道黏膜2次免疫鸡后,采用免疫组化的方法检测鸡十二指肠中生长抑素(Somatostatin,SS)阳性细胞和P物质(Substance P,SP)阳性细胞的分布及其数量变化。结果表明,动物首免后第3周,新城疫免疫组SS阳性细胞数量极显著增加(P<0.01),而SP阳性细胞数量显著减少(P<0.05);首免后第5周和第7周,新城疫免疫组和对照组中的2种阳性细胞数差异不显著。     

茶花鸡种群遗传结构分析及其保种措施的探讨

利用14个微卫星标记对云南茶花鸡12个家系的等位基因频率、平均基因杂合度、平均多态信息含量以及家系间的亲缘关系进行了分析。结果表明:共检测到99个等位基因,每个位点的等位基因分布并不均匀,再对12个家系进行分析,将其聚为3类,一类为Z-1,Z-2,Z-3,Z-4,Z-5,Z-6,B-1,一类为Z-7,Z-8,Z-9,Z-10,矮脚系B-2自成一类,这表明了家系间的遗传相似性,同时我们对特殊的矮脚系B-2进行了相关的探讨,最后我们提出了对茶花鸡保种的相关建议。     

灵芝多糖液体发酵条件优化

从一系列灵芝(Ganoderma sp.)菌种中筛选出多糖产量高、生长快的紫灵芝菌株(G.japoncium),研究了不同氮源、碳源及金属离子对紫灵芝产多糖的影响。结果表明,以2%的蔗糖为碳源,0.2%的豆饼粉为氮源,0.2%的FeSO4为培养基可获得较高多糖。发酵罐放大实验表明,采用同样的培养基,每100mL发酵液胞外粗多糖含量可高达181.7mg,每100mL发酵液菌丝体含量可高达151.0mg,发酵过程中pH值的变化比较缓和,相对摇瓶生长,发酵生产可获得更多的灵芝多糖。