核桃黑斑病综合防治技术

通过对核桃黑斑病发病侵染规律及原因分析,采取加强水肥土管理,合理修剪,减少密蔽,改善通风透光条件;谨防蛀果害虫;越冬管理;药剂防治的综合措施防治,可有效控制核桃黑斑病的发生危害。     

棉隆土壤消毒防除小麦田杂草的效果及对养分的影响

采用田间试验研究了98%棉隆微粒剂防除小麦田主要杂草的效果,及杂草防除后对氮、磷、钾及水分的影响。结果表明,施用98%棉隆微粒剂防除小麦田杂草效果显著,对菵草(Beckmannia syzigachne)、猪殃殃(Galium aparine L)、牛繁缕[Malachium aquaticum (L.) Fries]、大巢菜(Vicia sativa L.)均有良好防效,总鲜重防效达87.5%～99.5%,优于两种对照药剂。杂草防除后,显著降低了杂草对田间氮、磷、钾和水分的消耗,有效改善了田间的水肥条件。98%棉隆微粒剂处理的小麦田产量达2 970.01～3 123.77 kg/hm2,增产效果显著,产量较清水对照增加28.9%～35.6%,每公顷增收1 532.38～1 886.02元。     

辽宁省公众生态文明意识调查分析

通过对辽宁省不同人群进行问卷调查,结合层次分析法、专家打分法,获取评价指标权重,对辽宁省公众生态文明意识现状进行分析。结果表明,辽宁省公众生态文明意识整体较好,其中认同度践行度知晓度,表明辽宁省公民对生态文明的了解、认识与行为之间存在一定的差异。根据调查结果,提出相应建议,为辽宁省生态文明建设提供科学依据。     

Preparation of Atherosclerosis Model Using Guangxi Bama Mini-pig

The atherosclerosis (AS) model was prepared in Guangxi Bama mini-pig,and the atherosclerosis index (AI) was preliminarily identified in occurrence of AS to provide the basis for the preparation of related models.20 Guangxi Bama mini-pigs were randomly divided into control group and experimental group which were fed with high fat and cholesterol diet to prepare the AS model.Blood biochemical indexes were detected in the process of model preparation and the correlation between AI and the results of vascular slices was analyzed to initially draw up AI of AS.The results of vascular slices showed that the incidence rates of AS of Guangxi Bama mini-pigs in experimental group and control group were 20% and 0,respectively.The association analysis between the results of vascular slices and AI in the pathogenic Guangxi Bama mini-pigs preliminarily suggested that AI was above 3.8 and lasted for more than 3 months during the attack.     

Development of Nucleic Acid Lateral Flow Immunoassay Detection Method for Foot-and-mouth Disease Virus

In this article, through the combination of nucleic acid probes and immune chromatography, a simple, sensitive and specific detection system——nucleic acid lateral flow immunoassay (NALFIA) for amplifing foot-and-mouth disease virus (FMDV) 3D RT-PCR products was established.An ultrasensitive nucleic acid biosensor (NAB) based on streptavidin-labeled gold nanoparticles dual labels and lateral flow strip biosensor (LFSB) were used in this system.The biotinylated goat anti-rabbit IgG was marked to the NC membrane as the alleged strip and the anti-digoxin antibody was labeled to the NC membrane to capture the digoxin probe.After assemblying gold-labeled strip and detecting RT-PCR products, the detection limit of NALFIA was 0.3×10^-3 to 3×10^-3 μg/μL.The NALFIA was compared with agar gel electrophoresis analysis, the results showed that the sensitivity of NALFIA was higher than agar gel electrophoresis.There was an excellent agreement between the two methods.NALFIA was a method with high sensitive, low cost and short time.In conclusion, this method provided a good alternative to detect FMDV.     

酸性土壤中决明属牧草高效根瘤菌的分离及筛选

为提高豆科决明属（Chamaecrista spp.）牧草的结瘤能力和固氮效率，促进决明属牧草在酸性土壤中的应用及推广，本研究以圆叶决明（Chamaecrista rotundifolia）和羽叶决明（Chamaecrasta nictitans）为试验材料，通过分离、纯化、nodA基因鉴定等方法，从酸性土壤里决明属牧草的根瘤中分离到10株候选根瘤菌株系。16S rDNA测序及系统发育树分析表明，这10株根瘤菌均属于慢生型根瘤菌。回接试验表明，上述10株根瘤菌均能与圆叶决明共生，形成根瘤。与不接种的对照相比，其固氮酶活性为0.08~4.65 μmol·g^-1·h^-1，植株干重、SPAD值和氮含量分别提高了99.17%~372.14%，399.27%~789.05%，235.00%~1043.33%。其中，4株根瘤菌TXR2，TXN1，WYSR1，JYN6对圆叶决明氮营养贡献最大，并且TXN1与宿主共生形成的根瘤数目最多，而JYN6的固氮酶活性最高。说明本研究分离鉴定到4株决明高效根瘤菌，具有较好的应用前景。     

胡萝卜根中主要类胡萝卜素含量相关QTL的精细定位

以白色的野生胡萝卜‘松滋野生’(Ws)和橘色的栽培胡萝卜品种‘Amsterdam’(Af)为亲本构建的回交重组自交系(BIL)为试材,基于低倍重测序技术开发SNP标记,构建了由1 976个Bin标记组成,包含29 435个SNP标记的遗传图谱。图谱总距离834.28 c M,平均图距0.42 c M。通过对胡萝卜肉质根中类胡萝卜素含量相关QTL分析,在连锁群LG04和LG08中检测到调控α–胡萝卜素、β–胡萝卜素、ζ–胡萝卜素、叶黄素、玉米黄质和总类胡萝卜素含量的主效QTL(M-QTL)2、2、3、2、2和2个,表型贡献率为11.47%~19.18%;另检测到调控α–胡萝卜素、β–胡萝卜素、ζ–胡萝卜素、玉米黄质和总类胡萝卜素含量的上位性QTL(E-QTL)1、1、2、1和1个,表型贡献率为2.50%~3.66%。在M-QTL显著区间内共检索到36个有功能注释的预测基因,其中Dck018297为ζ–胡萝卜素脱氢酶2基因,与调控β–胡萝卜素合成和总类胡萝卜素含量有关;Dck008006为乙烯响应因子2.2的同源基因,与调控α–胡萝卜素、ζ–胡萝卜素合成有关;Dck029898为转录因子b HLH135的同源基因,与调控玉米黄质合成有关。