分子遗传技术在家畜遗传评定中的应用

基于表型信息和谱系信息估计基因加性效应值的种畜遗传评定方法在家畜遗传改良中发挥了很大作用,但因其无法真正了解控制经济性状的遗传本质,影响了家畜遗传改良的进一步进展。分子标记辅助选择可在一定程度上提高种畜遗传评定准确性,但在目前不能精确定位QTL或基因时,其效率受到很大影响。提高种畜遗传评定准确性的最有效途径应是直接利用控制经济性状的基因,后基因组时代的功能基因组研究的快速发展为实现这一目标提供了契机。同时,多个家畜品种基因组测序完成和大量SNP多态性的发现,使得利用覆盖全基因组多态性标记信息的基因组选择方法为家畜遗传评定开拓了又一条途径。     

Assessing whether dairy cow welfare is “better” in pasture-based than in confinement-based management systems

ABSTRACT

Consumers perceive pasture-based systems of milk production as natural and therefore better for cow welfare than confinement systems. However both systems are heterogeneous and continually evolving, varying from total confinement to total pasture with many hybrid intermediaries. To compare the welfare of dairy cows in these various systems, we use the three spheres framework, comprising biological functioning, natural behaviour and affective states. Considering biological functioning, pasture-based cows are less at risk of subclinical and clinical mastitis, claw lesions, lameness, metritis, early embryonic mortality, culling and mortality, but at more risk of internal parasitism, malnutrition and delayed onset of oestrous activity postpartum than confined cows. Regarding natural behaviours, pasture-based cows exhibit less agonistic behaviour, better lying behaviour, more normal oestrous behaviours and better synchronicity of behaviours than confined cows. They also have the opportunity to graze, which is one of the main features of the behavioural repertoire of dairy cows, but, they may also experience long periods away from pasture in larger herds, and severe climatic stresses which will become increasingly important as the climate changes. Our current ability to assess the affective state of dairy cows is poor. For example, hunger is an important subjective state that cannot be measured directly. The growing focus on ensuring that animals have lives worth living, means that dairy cows should garner some positive emotions from their lives, and it seems clear that pasture access is essential for this. Clearly measurement of affective state is an important challenge for future dairy cow welfare research. At the extremes of management systems, there can be major differences in animal welfare but in hybrid systems, dairy cows experience elements of both confinement and pasture which may ameliorate the negative effects of each on cow welfare. Ultimately, the optimal system gives cows an element of choice between both environments. Moreover management of the system, whether it is confinement or pastured-based, may be as important as the system of management in ensuring good dairy cow welfare and addressing societal concerns.

Abbreviations: BCS: Body condition score; TMR: Total mixed ration     

那曲东部土壤水分MODIS遥感反演研究

利用那曲东部2014年8月至2015年7月土壤水分观测资料与同期MODIS数据建立了研究区土壤水分遥感监测模型。表观热惯量法(ATI)反演土壤水分结果不理想,基于MODIS 8天合成数据以及晴空数据拟合结果的决定系数分别为0.4503和0.3753,晴空条件下ATI方法监测效果较差。基于单窗方法建立的四种模型中,三次多项式模型拟合效果较好,决定系数为0.5475,分析认为排除冬季数据后建模效果更好。结论:基于单窗方法的三次多项式模型能较好的反演研究区土壤水分,不足之处为对天气要求较高,若无晴空遥感数据,将影响土壤水分监测工作的开展。     

Use of azadirachtin as a larvicide or feeding deterrent for control of western flower thrips in orchard systems

Field trials were carried out to assess the efficacy of a 4% azadirachtin (aza)-containing neem seed extract EC, against damage by western flower thrips [Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae)] on nectarine crops in the Similkameen Valley, British Columbia, Canada. Several trials were conducted during the years 1993 to 1995 to assess the repellent and larvicidal properties of aza, specifically: whether it was effective against adult or larval thrips; how the effects differed with concentration of aza; and whether female WFT avoided aza-treated flowers. Our results showed that aza has only limited effectiveness as a control agent against WFT on nectarines and the current formulation does not appear to limit larval development sufficiently to be deemed a possible control agent in this field situation.     

5个日本枇杷品种植物学性状调查及果实品质比较

以大五星枇杷为对照,调查了引种四川的5个日本枇杷品种的植物学性状,测定果实维生素C、总酸、总糖及可溶性固形物等含量。结果表明,引进品种里见和有间综合性状表现最好,除可食率低于大五星外,单株产量与大五星无显著差异,单果重、维生素C、可溶性固形物含量等都高于大五星,适合在四川地区种植。     

典型黑土环境下的高、低镉积累白菜品种筛选及耐性比较

为探究不同白菜(Brassica pekinensis L.)品种在典型黑土环境中的镉敏感程度和镉积累特性,筛选出具备高镉、低镉积累特征的耐镉品种。试验以东北地区20种主栽白菜品种为材料进行盆栽模拟试验,研究基于不同白菜品种镉胁迫下的剂量-效应、镉积累特征,对得到的高、低镉积累品种的浓度积累效应及氧化损伤程度进行评估。结果显示:供试品种间剂量-效应差异明显,JF和BCCRQJ为镉耐性最强两个品种,耐受阈值分别为1.204 mg·kg~(-1)和1.036 mg·kg~(-1)。高镉浓度下(≥0.6 mg·kg~(-1))JF叶镉含量最低,根向叶迁移系数(TF)、叶富集系数(BCF)均小于1;BCCRQJ净化率最高。土壤镉含量与两者根、叶积累量、过氧化氢(H_2O_2)、超氧阴离子(O_2~-)、丙二醛(MDA)、游离脯氨酸(FPRO)均呈正相关,与净化率呈负相关(P0.05),此外高浓度下BCCRQJ氧化损伤程度较JF更高。综合试验结果表明,JF是镉耐性极高的低积累品种,而BCCRQJ为镉耐受力略逊于JF的高积累品种。     

安格斯牛与西藏犏牛杂交生产肉牛试验初报

[目的]犏牛可以生活在海拔2 800~4 200 m,对高寒缺氧的生态环境具有很强的适应能力,是“世界屋脊”的著名牛种,为了提高西藏犏牛资源的综合利用,其后代变废为宝。[方法]在试验区选择200头经产母犏牛通过引进安格斯牛的细管冻精,依靠人工授精技术,应用种间三元杂交技术生产优质商品肉牛试验,测定初生、3月龄的体重、体尺指标,观察高寒气候的适应性。[结果]结果显示:参配母犏牛200头,实配160头,其中104头母犏牛怀孕受胎,98头顺利产犊。实配率80%,人工授精受胎率65%,成活率94.23%。犏牛平均妊娠期(266.81±11.93) d。安格斯与西藏犏牛杂交后代的出生体尺、体重与传统杂交犊牛(多勒)相比,平均体重增加8.95 kg,提高73.23;体高、体斜长、胸围、管围平均分别比传统杂交犊牛(多勒)增加7.491 cm,1.394 cm,8.998 cm,0.063 cm,提高了13.94%,2.56%,15.70%,0.068%。安格斯与西藏犏牛杂交后代的3月龄与传统杂交犊牛(多勒)相比,平均体重增加43.3 kg,提高168.89;体高、体斜长、胸围、管围平均分别比传统杂交犊牛(多勒)增加15.67 cm,16.95 cm,26.31 cm,1.64 cm,提高23.27%,24.76%,34.31%,15.83%。[结论]弥补了犏牛传统杂交后代普遍存在的体躯发育差、生长发育慢等缺陷,且很好地适应西藏高寒生态环境和管理条件,杂种优势十分明显,因此安格斯牛可作为高原环境下繁殖犏牛生产肉牛的理想父本品种使用,并拥有开发高端产品的巨大潜力。     

铜、镉、铅、锌对4种豆科植物种子萌发的影响

筛选和研究适合当地气候与土壤条件的重金属耐性植物,是对重金属污染土壤进行植物修复的前提.以蒸馏水为对照,采用培养皿法研究了Cu2+、Cd2+、pb2+和Zn2+单一重金属对4种豆科植物种子萌发和幼苗生长的影响.结果表明,随着Cu2+、Cd2+、pb2+和Zn2+浓度的增大,红豆草(Onobrychis viciaefolia Scop.)、白三叶(Tri folium repens L.)、柠条(Caragana Korshinskii Kom.)、胡枝子(Lespedeza bicolor Turcz.)的发芽率、发芽势呈不同程度的降低趋势;但在Cu2+＜300 mg/L处理下,红豆草种子的发芽率并没受到明显抑制,反而有不同程度地促进;在Cd2+处理下,25 mg/L浓度对柠条种子萌发促进作用最为显著,柠条种子发芽率与对照相比达到显著性差异水平(p＜0.05);Pb2+对柠条和胡枝子种子的发芽率种子的发芽表现为“低促高抑”.Cu2+、Cd2+、pb2+和Zn2+对4种植物根系生长均表现出强烈的抑制作用.随着Cu2+、Cd2+、pb2+和Zn2+离子浓度的增大,红豆草、白三叶、柠条和胡枝子种子萌发后形成幼苗的根长和苗长都呈降低趋势,对4种植物均表现为随着浓度增加,抑制作用越明显.