全文获取类型
收费全文 | 134篇 |
免费 | 8篇 |
专业分类
林业 | 21篇 |
农学 | 6篇 |
基础科学 | 2篇 |
24篇 | |
综合类 | 16篇 |
农作物 | 11篇 |
水产渔业 | 13篇 |
畜牧兽医 | 44篇 |
园艺 | 2篇 |
植物保护 | 3篇 |
出版年
2022年 | 5篇 |
2021年 | 7篇 |
2020年 | 1篇 |
2019年 | 2篇 |
2018年 | 5篇 |
2017年 | 5篇 |
2016年 | 6篇 |
2015年 | 2篇 |
2014年 | 8篇 |
2013年 | 7篇 |
2012年 | 8篇 |
2011年 | 5篇 |
2010年 | 3篇 |
2009年 | 3篇 |
2008年 | 7篇 |
2007年 | 6篇 |
2006年 | 2篇 |
2005年 | 10篇 |
2004年 | 6篇 |
2003年 | 2篇 |
2002年 | 6篇 |
2001年 | 8篇 |
2000年 | 4篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1996年 | 3篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1967年 | 2篇 |
1966年 | 3篇 |
1960年 | 1篇 |
排序方式: 共有142条查询结果,搜索用时 0 毫秒
41.
Abnormal structure of the canine oncogene, related to the human c-yes-1 oncogene, in canine mammary tumor tissue. 总被引:1,自引:0,他引:1
N Miyoshi S Tateyama K Ogawa R Yamaguchi H Kuroda N Yasuda T Shimizu 《American journal of veterinary research》1991,52(12):2046-2049
Cellular oncogenes of genomic DNA in 6 canine primary mammary tumors were screened by Southern blot analysis, using 7 oncogene probes. A canine genomic oncogene related to the human c-yes-1 oncogene was detected as abnormal bands in solid carcinoma genomic DNA digested with EcoRI, HindIII, HindIII-EcoRI, or HindIII-BamHI. Comparison was made between other tumor specimens and control specimens obtained from 4 clinically normal dogs--1 mixed breed and 3 Shiba Inu dogs (the same breed as the dog from which the solid carcinoma was obtained). These abnormal bands were 0.1 to 1 kilobase shorter than the normal gene. However, digestion of genomic DNA obtained from normal WBC of this dog also produced all of the abnormal bands as observed in digested DNA from the solid carcinoma tissue. Therefore, in this dog, the genomic DNA of all somatic cells from the ontogenic stage still had the abnormal sequences related to the human c-yes-1 oncogene, and it is possible that this abnormal structure may have some role (eg, as an initiator) in tumorigenesis or the progression of this tumor. 相似文献
42.
Shiaki Takagi Lanlan Bai Tomomitsu Ozeki Hikaru Miyagi Kengo Kuroda Shunji Hayashi Hiroshi Yoneyama Tasuke Ando Emiko Isogai 《Animal Science Journal》2014,85(3):342-346
The cathelicidin family is one of the several families of antimicrobial peptides (AMPs). A bovine myeloid antimicrobial peptide (BMAP‐28) belongs to this family. Recently, the emergence of drug‐resistant bacteria such as methicillin‐resistant Staphylococcus aureus (MRSA) has become a big problem. AMPs are expected to be leading compounds of new antibiotics against drug‐resistant bacteria. In this study, we focused on the activity of BMAP‐28 against bacterial cell surfaces. First, we observed morphological change of MRSA caused by BMAP‐28 using a scanning probe microscope. We also studied activities of BMAP‐28 against adherence of S. aureus to fibronectin, collagen type I, collagen type IV. We confirmed whether BMAP‐28 can bind to lipoteichoic acid (LTA) of S. aureus. BMAP‐28 was indicated as damaging the cell surface of MRSA. In a particular range of concentrations, BMAP‐28 promoted adherence of S. aureus against fibronectin and collagens. It was revealed that BMAP‐28 and LTA of S. aureus bound with each other. Our study showed the potential of BMAP‐28 which can damage MRSA and interact with LTA of S. aureus but promote its adherence in some concentrations. This study provides new points of which to take notice when we use AMPs as medicines. 相似文献
43.
An increasing trend of Sr(90) concentration in rain observed at Fayetteville, Ark., after November 1959, indicates that the seasonal and global movements of stratospheric air masses, such as described by Brewer (I) and Dobson (2), play an important role in causing the spring peaks of the Sr(90) fallout. 相似文献
44.
Low-level activities of iodine-131, barium-140, and strontium-89 were found in a series of rain samples collected at Fayetteville, Arkansas, during the period from late June through August 1967. The ratios of these short-lived isotopes to strontium-90 were determined as accurately as possible. The data indicate that the debris from the Chinese nuclear explosion was injected primarily into the stratosphere. 相似文献
45.
Kuroda K Nishimura N Izumi A Dimmel DR 《Journal of agricultural and food chemistry》2002,50(5):1022-1027
Pyrolysis-gas chromatography in the presence of tetramethylammonium hydroxide (TMAH) was applied to the determination of the ratio of the abundances of the syringyl beta-aryl ether subunits to those of the guaiacyl equivalents (S/G) in lignin. Diazomethane-methylated kenafs (Hibiscus cannabinus and Hibiscus sabdariffa) and beech (Fagus crenata) in situ lignins were employed. Relative abundances of pyrolysis products derived from the guaiacyl and syringyl beta-aryl ether subunits were determined. The S/G ratios for in situ lignins were obtained with average 3.1% relative standard deviation for a minimum of six repeated runs. The S/G ratios determined by pyrolysis in the presence of TMAH agreed well with those determined by thioacidolysis, with a significant linear regression (R(2) = 0.9867). The results showed that pyrolysis with TMAH is an effective tool for obtaining information on the S/G ratio for in situ lignins. 相似文献
46.
Kuroda M Ohtake R Suzuki E Harada T 《Journal of agricultural and food chemistry》2000,48(12):6317-6324
To confirm the formation of gamma-glutamyl-beta-alanylhistidine and related peptide, a model solution (amide-containing amino acids and carnosine) has been heated, and the products are investigated. Spectroscopical analysis indicates that the major product from asparagine and carnosine is beta-aspartyl-beta-alanylhistidine, and that from glutamine and carnosine is gamma-glutamyl-beta-alanylhistidine. Furthermore, to confirm the increase of the above peptides during the heating process of food, an HPLC method for the determination of these isopeptides in food protein is constructed. The isopeptides are liberated by proteolytic digestion and fractionated by solid-phase extraction using Toyopack IC-SP cartridges. The fraction containing the isopeptides is derivatized with phenylisothiocyanate (PITC) and separated and quantified by HPLC using an octadecyl-silica column. As a result of quantification, an increase of the gamma-glutamyl-beta-alanylhistidine isopeptide in the macromolecular fraction of heated beef soup stock solution has been observed. These results suggest that the formation of the isopeptide occurs in the heating of various foods containing carnosine. 相似文献
47.
Summary Three breeding lines (F8) of rice, derived from cross between Brimful X Koshihikari were tested for protein, amino acid, growth test with rats and yield parameters in field trials. The breeding lines had higher protein and amino acid concentrations than the leading Japanese cultivar Koshihikari, which was grown as a control. Grain yield in the breeding lines was lower than that of Koshihikari (4.5 t/ha). The grain-protein yield of the breeding lines was higher (500 kg/ha) than that of Koshihikari (440 kg/ha). The average gain in weight of rats fed brown and milled rices of the breeding line was 19 and 21 g, respectively: in control rats fed Koshihikari average gain was –1 and 8 g. These results showed that the breeding lines of rice can be developed which possess higher food value than the available varieties. 相似文献
48.
49.
Kazuya Takeuchi Yusuke Kuroda Takamasa Numano Masayuki Kimura Seigo Hayashi Satoshi Furukawa 《Journal of toxicologic pathology》2021,34(3):269
Recently, intratracheal instillation has been focused on as a simple, low-cost alternative to the inhalation method. In this study, intratracheal instillation of sulfuric acid, a typical acidic compound, was performed to compare the acute toxicity of acidic compounds that could cause damage to the respiratory system between intratracheal instillation and inhalation. Sulfuric acid was administered to male rats at doses of 0.7, 2, 7, 20, and 60 mg/kg by dividing the total dose into four doses. General condition and body weight were examined up to 14 days after administration, and macropathological and histopathological examinations were performed. The half-lethal dose was then estimated. All animals administered 20 and 60 mg/kg sulfuric acid and one animal administered 2 mg/kg sulfuric acid died within 4 h after administration. No abnormalities were observed in other animals. At 20 and 60 mg/kg, multiple red foci or diffuse red areas were macroscopically observed in the lungs. In these lesions, histopathologically, clefts between the mucosal epithelium and basement membrane and necrosis of the alveolar epithelium were observed. Deaths in these groups may have resulted from lung injury. No notable changes were observed in other animals. Therefore, the half-lethal dose of sulfuric acid by intratracheal instillation was estimated as 7–20 mg/kg. The acute toxicity by intratracheal instillation was evaluated with two-fold sensitivity since the exposure at the half-lethal sulfuric acid concentration in inhalation studies was calculated as 43.2 mg/kg. 相似文献
50.
Kohei Matsushita Aki Kijima Yuji Ishii Shinji Takasu Meilan Jin Ken Kuroda Hiroaki Kawaguchi Noriaki Miyoshi Takehiko Nohmi Kumiko Ogawa Takashi Umemura 《Journal of toxicologic pathology》2013,26(1):19-27
In this study, the potential for development of an animal model (GPG46) capable of rapidly detecting chemical carcinogenicity and the underlying mechanisms of action were examined in gpt delta rats using a reporter gene assay to detect mutations and a medium-term rat liver bioassay to detect tumor promotion. The tentative protocol for the GPG46 model was developed based on the results of dose-response exposure to diethylnitrosamine (DEN) and treatment with phenobarbital over time following DEN administration. Briefly, gpt delta rats were exposed to various chemicals for 4 weeks, followed by a partial hepatectomy (PH) to collect samples for an in vivo mutation assay. The mutant frequencies (MFs) of the reporter genes were examined as an indication of tumor initiation. A single intraperitoneal (ip) injection of 10 mg/kg DEN was administered to rats 18 h after the PH to initiate hepatocytes. Tumor-promoting activity was evaluated based on the development of glutathione S-transferase placental form (GST-P)-positive foci at week 10. The genotoxic carcinogens 2-acetylaminofluorene (2-AAF), 2-amino-3-methylimidazo [4,5-f] quinolone (IQ) and safrole (SF), the non-genotoxic carcinogens piperonyl butoxide (PBO) and phenytoin (PHE), the non-carcinogen acetaminophen (APAP) and the genotoxic non-hepatocarcinogen aristolochic acid (AA) were tested to validate the GPG46 model. The validation results indicate that the GPG46 model could be a powerful tool in understanding chemical carcinogenesis and provide valuable information regarding human risk hazards. 相似文献