Disease-dependent differently methylated regions (D-DMRs) of DNA are enriched on the X chromosome in uterine leiomyoma

Uterine leiomyoma is the most common benign tumor in women. Although responsible gene mutations have not been found in leiomyomas, they represent a progressive disease with irreversible symptoms. To characterize epigenetic features of uterine leiomyomas, the DNA methylation status of a paired sample of leiomyoma and normal myometrium was subjected to a microarray-based DNA methylation analysis with restriction tag-mediated amplification (D-REAM). In the leiomyoma, we identified an aberrant DNA methylation status for 463 hypomethylated and 318 hypermethylated genes. Although these changes occurred on all chromosomes, aberrantly hypomethylated genes were preferentially located on the X chromosome. Using paired samples of normal myometrium and leiomyoma from 6 hysterectomy patients, methylation-sensitive quantitative real-time PCR revealed 14 shared X chromosome genes with an abnormal DNA hypomethylation status (FAM9A, CPXCR1, CXORF45, TAF1, NXF5, VBP1, GABRE, DDX53, FHL1, BRCC3, DMD, GJB1, AP1S2 and PCDH11X) and one hypermethylated locus (HDAC8). Expression of XIST, which is involved in X chromosome inactivation, was equivalent in the normal myometrium and leiomyoma, indicating that the epigenetic abnormality on the X chromosome did not result from aberration of XIST gene expression. Based on these data, a unique epigenetic signature for uterine leiomyomas has emerged. The 14 hypomethylated and one hypermethylated loci provide valuable biomarkers for understanding the molecular pathogenesis of leiomyoma.     

Cerebellar myxoid type meningioma in a Shih Tzu dog

A 6-year and 9 month-old, male, Shih Tzu dog showed ataxia and trembling. By MRI examination, a mass (1 cm) was found in the right cerebellum. As the dog did not respond to radiation therapy, and showed a rise of intracranial pressure, he was euthanized. The cerebellar mass was soft and hemorrhagic. Histologically, the mass contained vimentin-positive spindle- or polyhedral-shaped cells arranged in a cord-like pattern. Mucinous materials were observed in the intercellular spaces. Ultrastructural examination revealed cell processes, microtubule-like structures and desmosomes. The case was diagnosed as myxoid type meningioma.     

海水灌溉对Salicornia属两种盐生植物生长及离子吸收的影响

以Salicornin属的两个野生种为实验材料,在海水处理条件下对其地上部的生长状况及无机养分的吸收特性进行了分析.结果表明:随着海水处理浓度的增加,生物量表现出旺盛的生长趋势,100%海水处理区的生产量最高;两个野生种的地上部Na的含量和海水处理浓度之间有明显的正相关.Na在Salicornia属植物体内的积累阻碍K离子的吸收.淡水区S.bigelovii的Ca积累高于各盐水处理区,随着海水处理浓度的增加Ca的积累呈缓慢下降趋势.S.herbacea的Ca的积累呈相与S.bigelovii相反的趋势.Mg的积累和Ca的积累规律基本上相同.海水处理影响Salicornia属地上部氮和碳的积累过程.Salicornia属的两个野生种S.bigelovii和S.herbacea在海水处理下的生长及养分积累都表现出极高的趋同性.     

Termite feeding preference to four wood species after gamma irradiation

The effect of gamma irradiation at 100 kGy and at lower levels on termite resistance was examined in the laboratory by no-choice and choice feeding termite tests (Coptotermes formosanus Shiraki) using four wood species: sapwood of Cryptomeria japonica D. Don, and heartwoods of Pseudotsuga menziesii (Mirbel) Franco, Larix kaempferi (Lambert) Carriere, and Chamaecyparis obtusa Endl. The wood consumption rates in C. japonica and P. menziesii specimens were likely to increase with increases in gamma-irradiation levels, whereas little effect of gamma irradiation was seen in L. kaempferi and C. obtusa. Similar results were obtained in the two-choice test. The current results indicated that in the two-choice test with C. formosanus, 100-kGy-irradiated C. japonica and P. menziesii, which are not rich in antitermite substances, were eaten more than other wood samples with or without gamma irradiation. However, only C. japonica showed significant difference in termite feeding activity. The mass loss in 100-kGy-irradiated C. japonica was significantly higher in the multichoice test.     

Resistance of gamma-irradiated sapwood of <Emphasis Type="Italic">Cryptomeria japonica</Emphasis> to biological attacks

Any means helpful for the promotion of termite feeding activity has potential for use in a matrix in termite bait application. Therefore, energy transfer by gamma irradiation is worthy of consideration for converting wood into termite-accessible material. Wood specimens gamma-irradiated at 100 kGy and at lower levels were tested for their degrees of polymerization (DP) of cellulose and biological resistance. The DP of cellulose adversely decreased with increased doses of gamma irradiation. Termite wood consumption rates, which were determined by laboratory tests using undifferentiated larvae (workers) of Coptotermes formosanus Shiraki, were significantly higher at 100 kGy than at other doses. On the other hand, the decay resistance of gamma-irradiated wood against the fungi Fomitopsis palustris (Berkeley et Curtis) Murrill and Trametes versicolor (L. ex Fr.) Quel did not vary by irradiation dose.     

Role of sulphide reduction by magnesium hydroxide on the sediment of the eutrophic closed bay

This study was focused on the effect of magnesium hydroxide (Mg(OH)₂) for improving the sediment of aquaculture field and eutrophic closed estuaries. Sediment samples were collected from Omura Bay, Nagasaki, Japan. The change in pH, acid volatile sulphides (AVS) and dissolved organic carbon (DOC) in interstitial water of the sediment samples were measured before and after 20‐day incubation under the condition of overlapping seawater at 25°C with 0.3–0.4 mg/L of dissolved oxygen (DO). Hydrogen sulphide (H₂S) and the dsrA gene copy number of Grp3 sulphate‐reducing bacteria (SRB) in the sediment samples were also measured at 7‐day incubation under the same condition. The pH of Mg(OH)₂ treatment group was significantly increased from 7.43 to 8.25; it was higher compared to the control group which pH was maintained around 7.6. Meanwhile, the AVS content of Mg(OH)₂ treatment group was significantly reduced and the value was lower than the control group after the 20‐day incubation. Each concentration of interstitial water DOC in the treatment group and the control group increased after the incubation from initial value of 15.4–40.1 and 29.0  mg/L respectively. Furthermore, simultaneous decrease in H₂S and the dsrA gene copy number of Grp3 SRB was observed at the end of the 7‐day incubation by adding Mg(OH)₂. It is concluded that the loss of activity of SRB was strongly related to the decrease in sulphide (H₂S and AVS) and the increase in DOC was due to the alkalization of the sediment by adding Mg(OH)₂.     

Development of new self-feeding system for mass rearing of ayu <Emphasis Type="Italic">Plecoglossus altivelis altivelis</Emphasis> under artificial and natural light-dark cycles

Self-feeding of grouped ayu Plecoglossus altivelis altivelis was examined under an artificial light-dark (LD) cycle and natural day length using a trigger with a photosensitive sensor. In Experiment 1, approximately 15 juvenile fish (mean body weight, 0.6 g) were kept in 60-L glass tanks (four tanks) under LD 12∶12 (lighting period 06∶00–18∶00 hours) and self-feeding and locomotor activities were simultaneously recorded. Self-feeding was detected within one day and feeding activities were strongly synchronized to the LD cycle, with almost strictly diurnal feeding (98.2%). Although locomotor activities in the upper part of the tank were also greatest during the light phase (76.8%), the activity profiles were different from those of self-feeding, suggesting the validity of the photosensitive sensor. In Experiment 2,25 fish (mean body weight, 15 g) were kept in outdoor 1000-L tanks (four tanks) under natural day length and water temperatures in May, and self-feeding was recorded from mid-August. Stable self-feeding was observed from early September in all the tanks. Nearly all food demands were diurnal, and usually crepuscular. Somatic growth was seen in all tanks. These results demonstrate that the self-feeding system using a photosensitive sensor is applicable for grouped juvenile ayu as small as 0.6 g and adult fish reared under natural conditions.     

Changes in the immunostaining intensities of follicle-stimulating hormone and luteinizing hormone during ovarian maturation in the female Japanese flounder

The role of gonadotropin (GTH) in the reproduction of the Japanese flounder, Paralichthys olivaceus, was studied by assessing the changes in the apparent activity of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in the pituitary gland during gonadal maturation by immunohistochemical analyses. Corresponding changes in plasma levels of testosterone (T), estradiol-17β (E₂), and 17α,20β-dihydroxy-4–pregnen-3-one (DHP) were also studied. Reared fish at the early spawning to termination stages were sampled from May to August and wild fish at the previtellogenic to termination stages were caught at 3- to 4-week intervals between April and September offshore from the northern mainland of Japan by gill nets. The gonadosomatic index of the reared fish decreased from the early spawning stage to the termination stage, while that of the wild fish increased significantly from the previtellogenic stage to the early spawning stage and decreased thereafter. In the reared fish, the immunostaining intensities of FSH and LH were high during the spawning period, accompanied by high plasma levels of T, E₂, and DHP. In the wild fish, the immunostaining intensities of FSH and LH were low during the previtellogenic stage but increased during the maturing and spawning stages. These results indicate that both FSH and LH are likely associated with oocyte maturation in the Japanese flounder.     

Detection of porcine circovirus 2 (PCV-2) DNA by nested PCR from formalin-fixed tissues of post-weaning multisystemic wasting syndrome (PMWS) pigs in Thailand

A retrospective study for the detection of porcine circovirus 2 (PCV-2) DNA was conducted by nested PCR method in 16 cases of swine post-weaning multisystemic wasting syndrome (PMWS) in Thailand. Histopathology showed characteristic lesions of PMWS and intracytoplasmic viral inclusion bodies in macrophages infiltrating in lymphoid tissues. PCV-2 DNA was detected from formalin-fixed and/or formalin-fixed paraffin-embedded tissues from all pigs with PMWS. The amplified products were digested with Hae III.