Amelioration of Sodic Soil for Wheat Cultivation Using Bioaugmented Organic Soil Amendment

A novel bioaugmented organic amendment (SF_OA) [consisted of vermicompost (pre‐enriched with plant growth promoting fungi) mixed with pressmud and Azadirachta indica (A. Juss.) seed cake] was developed to reclaim sodic soil and support wheat production. A field trial of the SF_OA application with/without chemical fertilizers conducted in completely randomized design with four replications to compare growth, yield and seed protein contents of wheat (Triticum aestivum L.) on sodic soil. The favourable changes occurred in different properties of amended soils were studied. A combined application of chemical fertilizer and SF_OA significantly (p < 0·05) increased the number of spikelet per plant (63%) and weight of grains per ear (65%) in the amended soil compared with the control. Likewise, the grain yield, weight of 1000 grains and seed protein contents of wheat were significantly (p < 0·05) increased in the combined application compared with other treatments. The expression of protein bands with molecular weights of 36, 52 and 66 kDa were higher in seeds of wheat under the combined treatment. The improvement in wheat production was attributed to significant favourable changes in different soil characteristics such as bulk density, total organic C, alkaline phosphatase, β‐glucosidase, dehydrogenase and cellulase activities that were increased by 234%, 181%, 234%, 176%, 189% and 150%, respectively, in case of amended soil under the combined treatment compared with the control. The tested SF_OA may be recommended as soil amendment for reclaiming sodic soil and supporting wheat cultivation with better crop growth and yield in combination with chemical fertilizers on sodic soil. Copyright © 2014 John Wiley & Sons, Ltd.     

Effect of in ovo threonine supplementation on early growth,immunological responses and digestive enzyme activities in broiler chickens

1.?The effects of injecting threonine in ovo on early growth, some immunological responses and the activity of digestive enzymes of broiler chicks were investigated. Fertile eggs were distributed into 6 groups, each of 60. These were: untreated control, sham control, 10, 20, 30 or 40 mg threonine. Threonine was dissolved in 0·5 ml sterile saline and inoculated into the yolk sac of the 14-d-old embryo through the narrow end of the egg.

2.?The ratio of chick to egg weight was 1·6% higher in the group given 30 mg threonine and at 28 d of age chicks receiving threonine were 29 to 79 g heavier than untreated controls.

3.?Food conversion ratio until 7 d after hatching was improved in those chicks receiving 10, 20 or 40 mg threonine but there was no significant effect on the activities of amylase, pepsin or trypsin.

4.?The humoral response to sheep red blood cells was significantly greater in those groups receiving 10, 20 or 30 mg threonine supplementation than in untreated controls.

5.?The response to phytohaemagglutinin-P, a measure of the cell-mediated immune response, was not affected, however.

6.?It is concluded that injections of 20 to 30 mg threonine into yolk sac can improve post-hatching growth and humoral responses of broiler chicks.     

Comparative analysis of multi-criteria probabilistic FR and AHP models for forest fire risk(FFR) mapping in Melghat Tiger Reserve(MTR) forest

A comparative study of Frequency Ratio(FR)and Analytic Hierarchy Process(AHP)models are performed for forest fire risk(FFR)mapping in Melghat Tiger Reserve forest,central India.Identification of FFR depends on various hydrometeorological parameters altitude,slope,aspect,topographic position index,normalized differential vegetation index,rainfall,air temperature,land surface temperature,wind speed,distance to settlements,and distance by road are integrated using a GIS platform.The results from FR and AHP show similar trends.The FR model was significantly higher accurate(overall accuracy of 81.3%,kappa statistic 0.78)than the AHP model(overall accuracy 79.3%,kappa statistic 0.75).The FR model total forest fire risk areas were classified into five classes:very low(7.1%),low(22.2%),moderate(32.3%),high(26.9%),and very high(11.5%).The AHP fire risk classes were very low(6.7%),low(21.7%),moderate(34.0%),high(26.7%),and very high(10.9%).Sensitivity analyses were performed for AHP and FR models.The results of the two different models are compared and justified concerning the forest fire sample points(Forest Survey of India)and burn images(2010-2016).These results help in designing more effective fire management plans to improve the allocation of resources across a landscape framework.     

Calibration and performance of moisture sensors in soilless substrates: ECH2O and Theta probes

Reliable and affordable moisture sensors for measuring the water content in soilless substrates are limited. In this study, we examined the efficacy of two moisture sensors (ECH₂O-10 and Theta probe ML2X) for measuring water content in soilless substrates. We developed calibration equations and analyzed the effect of increasing electrical conductivity (EC) and substrate temperature on the voltage output of probes. We found that a single equation (one for each probe) could be used to adequately measure water content in different custom-made substrates maintained at low EC and a substrate temperature of ∼23 °C. The calibration equation developed for the Theta probe from substrates maintained at low EC could also be used in two commercial substrates with high EC (2.0–5.0 dS m⁻¹). Further measurements in substrates maintained at different water contents and EC levels indicated that the output of the ECH₂O-10 probe, but not the Theta probe, was significantly affected by substrate EC. The lack of a substrate EC effect on the Theta probe readings was surprising, because the probe was sensitive to EC when fertilizer solutions with different ECs were measured. Increasing the temperature of the substrate from 10 to 40 °C increased the voltage output of ECH₂O-10 probes by 1.88 mV °C⁻¹, or on average 0.0022 m³ m⁻³ water content per °C. There was no effect of increasing substrate temperature on the Theta probe. It was concluded that ECH₂O-10 probes can be used in greenhouse operations requiring less measurement precision (like irrigation), however for accurate measurements of water content, the Theta probe is preferred.     

Alteration in testicular cyclic AMP system in the rat following vasectomy.

Endogenous cyclic AMP levels and the activities of adenylate cyclase, cyclic AMP-dependent and independent protein kinases were examined in testes of mature rats bilaterally vasectomized for one, three and seven months. Although no significant alteration in testicular cyclic AMP was detected one month following vasectomy, marked decreases (by 55% and 32%, respectively) were seen three and seven months postvasectomy. Likewise, vasectomy also resulted in a significant decrease (by 25%) in the activity of testicular adenylate cyclase three and seven months after vasectomy. Although soluble cyclic AMP-dependent protein kinase activity remained unaffected three months postvasectomy, the activity of the cyclic nucleotide-dependent enzyme was significantly increased (by 21%) when compared to the sham-operated controls. Furthermore, while the protein kinase ratio (--cyclic AMP/+cyclic AMP) was decreased in animals vasectomized for three months, the ability of the enzyme to bind (3H) cyclic AMP in vitro was significantly enhanced (18%). Rats vasectomized for seven months showed similar biochemical alterations but the effects of this procedure were more pronounced. Moreover, while short-term vasectomy increased the responsiveness of seminiferous tubular adenylate cyclase to in vitro stimulation by follicle stimulating hormone, the activity of the enzyme was also increased (by 100%) in the presence of luteinizing hormone in vasectomized rats. These data raise the possibility that changes in testicular function seen following vasectomy may be related to the alterations in cyclic AMP metabolism as well as in the sensitivity of testicular adenylate cyclase to regulation by gonadotropins.     

Standardization of leaf-sampling technique for mineral composition of leaves of mango cultivar ‘Chausa’

The effects of leaf age, leaf position on the shoot, sampling-direction and sampling-height of leaves of mango cultivar ‘Chausa’ on their mineral composition were studied. The P and K contents decreased, while Ca, Mg, S and Mn contents increased significantly with advancing age of leaves. In general, N, P, Mg, S, Zn, Mn and Fe contents were stable in 6- to 7-months-old leaves. P, K, Ca, Mn and Fe contents varied with sampling-direction, while K, Ca, S, Zn, Cu and Fe contents were significantly higher in the leaves from the lower portion than from the upper portion of the tree. A continuous increase in Ca and Mg contents and decrease in K and Mn contents of leaves were observed from basal to terminal leaves on a shoot. It was concluded that 6- to 7-months-old leaves from the middle of non-fruiting shoots, sampled from all directions and heights, were most suitable for assessing the nutritional status of a mango tree.     

Rate of infection and of reinfection by Echinococcus granulosus in rural dogs of the province of Rio Negro, Argentina

Hydatidosis (cystic echinococcosis) constitutes a serious public health problem in the Province of Rio Negro, Argentina. In the present work it was intended to evaluate the prevalence of the canine echinococcosis in rural areas of the Province of Rio Negro and studied the reinfection rate in dogs after treatment with Praziquantel during the period 1980-1997. A total of 496 dogs were studied in 18 canine concentrations in order to establish the initial prevalence rate which was 42.3%. From 1980 onward dogs should have been systematically treated with anthelmintic every 2 months in rural areas and every 6 months in urban areas. We estimated that approximately 65% of dogs were treated. To determine the reinfection rate, 476 dogs (1984) and 598 dogs (1996) were studied after anthelmintic treatment during two sequential time periods (0-45; 46-90 days). In both cases treated animals were compared with untreated dogs. Prevalences were 3.5%, 6.7% and 21.3% in 1984 and 0.8%, 4.0% and 10.0% in 1996. For the purpose of surveillance a total number of 21,444 dogs were studied during 18 years. Prevalence of Echinococcus granulosus decreased significantly in the first year from 42.3% to 6.1%.     

HCV persistence and immune evasion in the absence of memory T cell help

Spontaneous resolution of hepatitis C virus (HCV) infection in humans usually affords long-term immunity to persistent viremia and associated liver diseases. Here, we report that memory CD4+ Tcells are essential for this protection. Antibody-mediated depletion of CD4+ Tcells before reinfection of two immune chimpanzees resulted in persistent, low-level viremia despite functional intra-hepatic memory CD8+ Tcell responses. Incomplete control of HCV replication by memory CD8+ Tcells in the absence of adequate CD4+ Tcell help was associated with emergence of viral escape mutations in class I major histocompatibility complex-restricted epitopes and failure to resolve HCV infection.     

Penetration of human-induced warming into the world's oceans

A warming signal has penetrated into the world's oceans over the past 40 years. The signal is complex, with a vertical structure that varies widely by ocean; it cannot be explained by natural internal climate variability or solar and volcanic forcing, but is well simulated by two anthropogenically forced climate models. We conclude that it is of human origin, a conclusion robust to observational sampling and model differences. Changes in advection combine with surface forcing to give the overall warming pattern. The implications of this study suggest that society needs to seriously consider model predictions of future climate change.     

Optimization of simultaneous saccharification and fermentation for the production of ethanol from lignocellulosic biomass

Simultaneous saccharification and fermentation (SSF) of alkaline hydrogen peroxide pretreated Antigonum leptopus (Linn) leaves to ethanol was optimized using cellulase from Trichoderma reesei QM-9414 (Celluclast from Novo) and Saccharomyces cerevisiae NRRL-Y-132 cells. Response surface methodology (RSM) and a three-level four-variable design were employed to evaluate the effects of SSF process variables such as cellulase concentration (20-100 FPU/g of substrate), substrate concentration (5-15% w/v), incubation time (24-72 h), and temperature (35-45 degrees C) on ethanol production efficiency. Cellulase and substrate concentrations were found to be the most significant variables. The optimum conditions arrived at are as follows: cellulase = 100 FPU/g of substrate, substrate = 15% (w/v), incubation time = 57.2 h, and temperature = 38.5 degrees C. At these conditions, the predicted ethanol yield was 3.02% (w/v) and the actual experimental value was 3.0% (w/v).