Spectrophotometric method for differentiation of cardiac and hepatic lactate dehydrogenase activities in dogs

To differentiate the origin of high total lactate dehydrogenase (LD) activity in canine sera, a spectrophotometric method based on the preferential inhibition of cardiac LD isoenzymes by pyruvate was performed. Comparison with the electrophoretic separation of LD isoenzyme activities and determination of the hydroxybutyrate dehydrogenase-to-LD ratio indicated that the method proposed gave a better discrimination between cardiac and hepatic LD activities than did the other tests.     

Effect of clear-felling on runoff in two small watersheds in Central Sweden

Small watersheds were used in a study of the effect of clear-felling on runoff from coniferous forest areas in Central Sweden. After a 3-year calibration period, two watersheds were clear-felled (on one of them the slash was left and on the other slash was removed) and a third was left as a reference.Runoff under forested conditions was 271 and 246 mm year^?1, respectively for the treated watersheds, while the precipitation measured 732 mm year^?1 as a mean for the investigated period. Clear-felling resulted in an increase in runoff of 119 and 75% (371 and 215 mm per year), respectively. The increase occurred during the period of snowmelt (April–May) and the period without snow (June–October). During winter (November–March) a very small increase in runoff was noted. The effect of clear-felling on runoff appears to be greater than has been reported from most other Scandinavian investigations.The increase in runoff during snowmelt is probably due to an increased snow accumulation in the clear-felled area as compared to the forest. The increased runoff for the period without snow is explained by reduced transpiration from the vegetation and reduced evaporation caused by an increased aerodynamic resistance.No effects of slash removal could be detected, probably because of the natural variation between the two treated watersheds.     

Economic evaluation of heterosis and culling policies for lifetime productivity in Hereford, Angus, shorthorn, and crossbred cows.

Experimental lifetime performance data obtained from 156 straightbred Hereford, Angus, and Shorthorn and 172 first-cross heifers were used to estimate heterosis for economic efficiency in a 100-cow herd at age equilibrium under three culling policies and at terminal ages from 6 to 12 yr. All nonpregnant heifers and cows greater than 9 yr of age were culled. The culling policy for removal of nonpregnant cows from second parity through 9 yr of age were 1) no culling, 2) after two consecutive years (actual), and 3) all (imposed). Efficiency was calculated as input cost per unit of output value. A 10-yr average was used for costs of replacement heifers, cow units, and the ratio of calf:cull cow prices (PR), plus higher and lower PR. Input included costs for both cow units and purchased replacements. Output value included both weaned calves and cull cows. Optimum terminal age was mainly a function of PR: 9 yr for average and high PR, but 6 through 9 yr when PR was low, regardless of culling policy or breed groups. Efficiency differences among culling policies were small for high or average PR, but more culling for infertility was beneficial when PR was low. Estimated reductions in unit costs of output value under any culling policy or terminal age were approximately 6% from crossbred cows plus another 6% from crossbred calves, or a total of 12% from specific three-breed crossing of these British breeds. Cost reductions would be somewhat less for rotation crossbreeding but greater for mating smaller crossbred cows with sires of superior growth-carcass breeds.     

An ELISA with Brucella lipopolysaccharide antigen for the diagnosis of B. melitensis infection in sheep and for the evaluation of serological responses following subcutaneous or conjunctival B. melitensis strain Rev 1 vaccination.

An indirect enzyme-linked immunosorbent assay (ELISA) with unpurified Brucella melitensis smooth lipopolysaccharide (S-LPS) as antigen was evaluated for the serological diagnosis of B. melitensis infection in sheep in comparison with the Rose Bengal (RB), complement fixation (CF), radial immunodiffusion (RID), microplate agglutination (MA) and rivanol agglutination (RIV) tests. Tests RB and CF detected as positive each of the 77 sera from B. melitensis-infected animals tested, the RID (74), MA (76) and the RIV (72) were less sensitive. However, all tests compared were negative when 77 sera from Brucella-free rams were tested. While subcutaneous Rev 1 vaccination induced high response levels in any of the tests, low level responses were obtained upon conjunctival vaccination, particularly in ELISA and RID tests.     

Studies on the antifungal action of potential steroid inhibitors

Structure-activity relationships were studied with macrocyclic esters of N,N′-ethylenebis-(2,2′-amino-1,1′-cyclopentene-1,1′-dithiocarboxylic acid), with compounds related to α-(2,4-dichlorophenyl)-α-phenyl-5-pyrimidinemethanol (triarimol) and with N-substituted imidazoles, all known or supposed to exert antifungal action by inhibiting the function or biosynthesis of fungal sterols.