全文获取类型
收费全文 | 307篇 |
免费 | 4篇 |
国内免费 | 2篇 |
专业分类
林业 | 34篇 |
农学 | 3篇 |
基础科学 | 2篇 |
48篇 | |
综合类 | 5篇 |
农作物 | 6篇 |
水产渔业 | 64篇 |
畜牧兽医 | 132篇 |
园艺 | 6篇 |
植物保护 | 13篇 |
出版年
2022年 | 5篇 |
2021年 | 13篇 |
2020年 | 3篇 |
2019年 | 5篇 |
2018年 | 3篇 |
2017年 | 3篇 |
2016年 | 3篇 |
2015年 | 10篇 |
2014年 | 20篇 |
2013年 | 16篇 |
2012年 | 17篇 |
2011年 | 21篇 |
2010年 | 14篇 |
2009年 | 20篇 |
2008年 | 12篇 |
2007年 | 26篇 |
2006年 | 20篇 |
2005年 | 20篇 |
2004年 | 15篇 |
2003年 | 20篇 |
2002年 | 13篇 |
2001年 | 2篇 |
2000年 | 7篇 |
1999年 | 2篇 |
1998年 | 3篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1988年 | 2篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1955年 | 1篇 |
1952年 | 1篇 |
1949年 | 1篇 |
排序方式: 共有313条查询结果,搜索用时 31 毫秒
231.
Tanaka T Abe Y Inoue N Kim WS Kumura H Nagasawa H Igarashi I Shimazaki K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(6):619-625
Trypanosoma brucei, the causative agent of sleeping sickness in humans, requires transferrin (TF) for growth. Therefore, T. brucei has a TF receptor that allows it to obtain iron from TF. Lactoferrin (LF), a member of the TF family protein, is an iron-binding protein that is found in most biological fluids of mammals. LF has been shown to interact with some bacteria species by specific receptor-ligand binding. We examined the ability of T. brucei to bind bovine LF (bLF) by using a fluorescence test, streptavidin-biotin (SAB) microplate analysis, and far Western blotting using a biotin-streptavidin system. We found that bLF bound to components of T. brucei, and that bLF hydrolysate disrupted the sites responsible for binding to parasite proteins. Furthermore, bLF, human LF, bovine TF, and ovotransferrin bound same proteins of T. brucei, which exhibited molecular masses of 40 and 43 kDa. The N-terminal amino acid sequence of the 40 kDa bLF binding protein was identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). 相似文献
232.
Verdida RA Hara OA Xuan X Fukumoto S Igarashi I Zhang S Dong J Inokuma H Kabeya H Sato Y Moritomo T Maruyama S Claveria F Nagasawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(12):1517-1521
The surface antigen P50 of Babesia gibsoni is an important candidate for the development of a diagnostic reagent for canine piroplasmosis. In order to establish an effective diagnostic method for practical use, the gene encoding truncated P50 (P50t) lacking a signal peptide and C-terminal hydrophobic regions were cloned and expressed in Escherichia coli as a fusion protein with glutathione S-transferase (GST). More than 90% portion of the GST-P50t was expressed as a soluble form, in contrast with GST-P50f (full-length), which was completely expressed as an insoluble form. This result indicates that removal of the hydrophobic signal peptide and C-terminus had dramatically improved its hydrophilicity. The purified GST-P50t was tested in an enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to B. gibsoni in dogs. The ELISA with GST-P50t clearly differentiated between B. gibsoni-infected dog sera and uninfected dog sera. In addition, the ELISA detected no cross-reactivity with sera from dogs experimentally infected with the closely related parasites, B. canis canis, B. canis vogeli, and B. canis rossi. Field serum samples collected from dogs in Japan and China were examined for the diagnosis of B. gibsoni infection by using the ELISA. 14.5% (9/62), 5.8% (7/120), and 5.4% (2/37) of tested samples were positive for dogs from Okinawa, Yamaguchi, and Osaka prefectures, Japan, respectively. On the other hand, 4.8% (2/41) of tested samples were positive for dogs from Nanjing, China. These results suggest that the GST-P50t could be a reliable reagent for practical use in ELISA for the serodiagnosis of canine piroplasmosis caused by B. gibsoni. 相似文献
233.
Yahara Y Ohkubo Y Kariwa H Takashima I 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(7):583-588
To evaluate the immunofluorescent antibody (IFA) test and enzyme-linked immunosorbent assay (ELISA) for detecting the porcine reproductive and respiratory syndrome virus (PRRSV) antibody, conventional pigs in PRRSV-positive and -negative commercial farms were examined. Antibody development patterns in ELISA and IFA tests were compared in 3 week old piglets experimentally infected with the PRRSV. The virus was detected from 2 days post infection (PI) and then the antibody titers and S/P ratios rose by both methods. A total of 208 serum samples were collected from 4 PRRSV-negative farms and 210 samples from PRRSV-positive farms, and were tested for the PRRSV antibody by IFA and ELISA. The titer of 64 should be set as the cut-off point in IFA for field sera. Similarly, the cut-off S/P ratio should be set at 0.4 in ELISA. A high degree of correlation was observed between antibody titers by the two methods in these 418 samples, with a correlation coefficient of 0.84. The coincidence rate between the two tests was 84.7% (354/418). In non-coincident cases, ELISA was able to detect the antibody with a low titer in the serum samples which were negative in IFA but from PRRSV positive farms. ELISA was more sensitive than IFA to detect PRRSV infected animals or farms. 相似文献
234.
Battsetseg B Lucero S Xuan X Claveria F Byambaa B Battur B Boldbaatar D Batsukh Z Khaliunaa T Battsetseg G Igarashi I Nagasawa H Fujisaki K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(8):727-730
Babesia equi (EMA-1) and Babesia caballi (BC48) gene fragments were amplified by polymerase chain reaction (PCR), in blood samples, and partially fed-females and egg and larval progenies of Dermacentor nuttalli, collected from horses in Altanbulag, Tuv Province, Mongolia. While Babesia parasite DNA was detected in some horse blood samples during the first PCR, all positive cases in partially fed-female ticks, eggs and larvae were confirmed by nested PCR. Present study reinforces earlier similar findings in unfed D. nuttalli ticks collected from an open space vegetation in Bayanonjuul, Tuv Province in Central Mongolia, pointing to the most likely important role of D. nuttalli in the transmission of equine babesiosis in Mongolia. The detection of parasite DNA in eggs and larval progenies is likewise suggestive of transovarial parasite transmission in this tick species. 相似文献
235.
Kariwa H Noda H Nakauchi M Ishizuka M Hashiguchi K Hashimoto S Yoshii K Asano A Agui T Kogaki H Kurano Y Uchida Y Fujii N Okada M Takashima I 《The Japanese journal of veterinary research》2008,55(4):115-127
The sudden emergence of severe acute respiratory syndrome (SARS) at the end of 2002 resulted in 774 reported deaths from more than 8000 cases worldwide. As no effective vaccines or antiviral agents are available, the most effective measure to prevent the expansion of a SARS epidemic is the rapid diagnosis and isolation of SARS patients. To establish specific diagnostic methods, we generated nine clones of monoclonal antibodies to nucleocapsid protein (NP) of SARS-coronavirus (SARS-CoV). On immunofluorescent antibody assay and Western blotting analysis, none of the monoclonal antibodies showed cross-reactivity to authentic and recombinant NPs of human coronavirus (HCoV) 229E strain. To determine the region on the NP molecule where the monoclonal antibodies bind, we generated four truncated recombinant NPs and analyzed the reactivity between monoclonal antibodies and truncated NPs. Two monoclonal antibodies reacted with a truncated NP covering from amino acid residues 111 to 230, and seven reacted with another truncated NP covering from amino acid residues 221 to 340. Epitope mapping analysis indicated that monoclonal antibody SN5-25 recognized the amino acid sequence Q(245)TVTKK(250) On SARS-NP. Within the epitope, Q245, T246, V247, K249, and K250 appeared to form an essential motif for monoclonal antibody SN5-25 to bind. The information about binding sites and epitopes of monoclonal antibodies may be useful for the development of new diagnostic methods for SARS and for analyzing the function of N protein of SARS-CoV. 相似文献
236.
Effect of feeding sweet‐potato condensed distillers solubles on intake and urinary excretion of minerals in Japanese Black steers
下载免费PDF全文
![点击此处可从《Animal Science Journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Yuko Kamiya Misturu Kamiya Ikuo Hattori Yoshiro Hayashi Masayuki Funaba Tohru Matsui 《Animal Science Journal》2017,88(1):79-85
Four Japanese Black steers (16 months of age) were assigned to a 4 × 4 Latin square design to investigate the effect of graded levels of sweet‐potato condensed distillers solubles (SCDS) in their diets on intake and urinary excretion of minerals. The four diets consisted of 0%, 10%, 20% and 30% (dry matter (DM) basis) SCDS, with SCDS replacing commercial concentrate (CC). Intake of K, Cl, S, P and Mg increased linearly with increasing SCDS content. Urinary pH increased linearly with increasing dietary SCDS content. SCDS feeding increased urinary K concentrations (linear and quadratic effects). Urinary concentrations of Cl increased linearly with increasing SCDS content. In contrast, urinary concentrations of Mg decreased with increasing SCDS content. Feeding of SCDS did not apparently affect urinary NH3,P, Na or Ca concentrations. These results suggest that high SCDS feeding is not a risk for crystallization of minerals leading to the formation of magnesium‐phosphate type calculi: although SCDS contains large amounts of P and Mg, high SCDS feeding decreased the Mg concentration and did not affect the P concentration in urine. Additionally, high SCDS feeding had no apparent effects on plasma concentrations of Na, K, Cl, Ca or inorganic P. 相似文献
237.
238.
A convenient method which possessed simplicity and high sensitivity was designed to investigate the changes in free fatty acid (FFA) of rice during storage using a thin‐layer chromatography and flame‐ionization detection (TLC/FID) system. In this method, two different solvent systems for TLC were used according to the purpose of experiments. Solvent system A (hexane and diethyl ether and acetic acid 80:20:1) was suitable to obtain a chromatogram showing the overall state of rice lipid degradation. Using solvent system A, the degradation of triglyceride or the increase in FFA during storage was clearly visualized as changes in the chromatogram. Solvent system B (hexane and acetic acid 100:1) was used to improve the low reproducibility of the TLC/FID method. When methyl stearate was used as an internal standard with solvent system B, high reproducibility of the FFA value was obtained, and very small changes were detectable in stored white milled rice. This method has small sample size and simple operation and is more sensitive than the standard titration method. Therefore, this seems to be an especially convenient method for small‐scale storage tests or for experiments using many samples. 相似文献
239.
Yuna Han Gen Kaneko Reiko Nagasaka Hidehiro Kondo Ikuo Hirono Shin-Ichiro Takahashi Shugo Watabe Hideki Ushio 《Fisheries Science》2013,79(1):143-148
Adiponectin is one of the adipokines secreted mainly from adipocytes in mammals. In rainbow trout, however, adiponectin is highly expressed in skeletal muscle. Although it has been suggested that fish skeletal muscle contains adipocytes, their endocrine function and distribution are poorly understood. Recently, an EST analysis of rainbow trout found that heart-type fatty acid binding protein (H-FABP), a member of the intracellular fatty acid binding protein family, encodes an adipose-specific gene. In this study, we produced anti-adiponectin and H-FABP antibodies and investigated the distribution of adipocytes and related cells in skeletal muscle of rainbow trout. The adiponectin signal was detected at around 75 kDa in muscle in Western blotting. Since the molecular mass of rainbow trout adiponectin is around 25 kDa, this 75 kDa band would be a trimer. For H-FABP, the signal band was detected at around 15 kDa. Immunohistochemistry of skeletal muscle sections indicated that adiponectin and H-FABP signals were present outside of muscle cells and throughout the muscle tissues, suggesting the existence of adipocyte-related cells in these regions. These results will contribute to our understanding of energy metabolism in fish skeletal muscle. 相似文献
240.