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101.
Mano J Yanaka Y Ikezu Y Onishi M Futo S Minegishi Y Ninomiya K Yotsuyanagi Y Spiegelhalter F Akiyama H Teshima R Hino A Naito S Koiwa T Takabatake R Furui S Kitta K 《Journal of agricultural and food chemistry》2011,59(13):6856-6863
Because of the increasing use of maize hybrids with genetically modified (GM) stacked events, the established and commonly used bulk sample methods for PCR quantification of GM maize in non-GM maize are prone to overestimate the GM organism (GMO) content, compared to the actual weight/weight percentage of GM maize in the grain sample. As an alternative method, we designed and assessed a group testing strategy in which the GMO content is statistically evaluated based on qualitative analyses of multiple small pools, consisting of 20 maize kernels each. This approach enables the GMO content evaluation on a weight/weight basis, irrespective of the presence of stacked-event kernels. To enhance the method's user-friendliness in routine application, we devised an easy-to-use PCR-based qualitative analytical method comprising a sample preparation step in which 20 maize kernels are ground in a lysis buffer and a subsequent PCR assay in which the lysate is directly used as a DNA template. This method was validated in a multilaboratory collaborative trial. 相似文献
102.
The mammalian vomeronasal organ detects complex chemical signals that convey information about gender, strain, and the social and reproductive status of an individual. How these signals are encoded is poorly understood. We developed transgenic mice expressing the calcium indicator G-CaMP2 and analyzed population responses of vomeronasal neurons to urine from individual animals. A substantial portion of cells was activated by either male or female urine, but only a small population of cells responded exclusively to gender-specific cues shared across strains and individuals. Female cues activated more cells and were subject to more complex hormonal regulations than male cues. In contrast to gender, strain and individual information was encoded by the combinatorial activation of neurons such that urine from different individuals activated distinctive cell populations. 相似文献
103.
Thanh-Hao Huynh Su-Ying Chien Junichi Tanaka Zhi-Hong Wen Yang-Chang Wu Tung-Ying Wu Ping-Jyun Sung 《Marine drugs》2021,19(3)
Chemical investigation of the octocoral Briareum stechei, collected in the Ie Island, Okinawa, Japan, resulted in the isolation of a new briarane-type diterpenoid, briastecholide A (1), as well as the previously reported metabolites, solenolide C (2) and briarenolide S (3). The structures of briaranes 1–3 were characterized through spectroscopic analysis, and the absolute configuration of 2 was corroborated by a single-crystal X-ray diffraction analysis. Briarane 3 exhibited bioactivity against the protein expression of inducible nitric oxide synthase (iNOS). 相似文献
104.
Junichi Yamaguchi 《Soil Science and Plant Nutrition》2013,59(2):155-161
Four highland tillering varieties were grown on a high plateau in Mexico with three plant densities to examine the contribution of tillers to the grain yield and the following results were obtained: 1) At low densities tillers were as efficient as the main culm in grain production, and grain yield attributed to the tillers was nearly 50 per cent of the total grain yield. However, at high densities tillers were less effective. The contribution of tillers to the total yield was only a few per cent. 2) The proposition was discussed that tillering ability was an important character for obtaining a reasonably high yield at low densities, but was not an important character in seeking a maximum grain yield with a high plant density. 相似文献
105.
Junichi Yamaguchi 《Soil Science and Plant Nutrition》2013,59(2):367-383
Abstract It is well known that stem nodules are formed on the aerial parts of Aeschynomene spp. and Sesbania rostrata grown in the field (Yatazawa and Yoshida 1979; Dreyfus and Dommergues 1981; Yoshida et al. 1985). We have reported that stem nodules were successfully formed by inoculation of Rhizobium isolates derived from both stem and root nodules of A. indica (Yoshida et al. 1985; Sasakawa et al. 1986). The specific activity of nitrogen fixation in stem nodules is comparable to that of root nodules (Sasakawa et al. 1986; Sasakawa 1990). A red pigment, which suggests the presence of leghemoglobin, was detected in stem nodules as well as in root nodules (Yatazawa and Yoshida 1979; Yatazawa and Susilo 1980; Sasakawa et al. 1986). 相似文献
106.
Abstract It is generally considered that the larger the volume of soil in which the root system of a crop develops, the better the development of roots and the growth of the crop. This trend is attributed to (a) the larger quantity of water and nutrients available to the crop and (b) better supply of oxygen to the roots with the increase of the soil volume. There is, however, no information on the effect of the physical space itself on root development and plant growth. 相似文献
107.
Kyohei Yasuno Haruka Sakashita Ryosuke Kobayashi Saori Araki Rio Saito Mariko Shirota Junichi Kamiie Kinji Shitora 《Journal of toxicologic pathology》2013,26(2):203-208
Membranous glomerulonephropathy can be experimentally induced in rats, but spontaneous
cases have been rarely reported. In this report, we present a typical case of spontaneous
membranous glomerulonephropathy in a rat. A male Hatano low-avoidance (LAA) strain rat had
a tumor mass on the right auricle, and was sacrificed at 41 weeks of age. Urinary
screening by reagent strips revealed intense proteinuria. Histological tests revealed
frequent presence of irregularly sized eosinophilic hyaline materials on the capillary
wall and in the mesangium of renal glomeruli. Immunofluorescence revealed granular
deposits of IgG, IgM, and C3 in the glomeruli. Subepithelial dense deposits were observed
by electron microscopy accompanied by podocyte foot process effacement and occasional
irregular thickening of the glomerular basement membrane. The rat also developed chronic
lymphocytic pancreatitis, and the tumor mass on the right auricle was diagnosed as a
fibrosarcoma. Screening tests for antibodies against major infectious agents and
antinuclear antibody were negative. Western blot and indirect immunofluorescence analyses
suggested the presence of an autoantibody against the pancreatic component. The
glomerulopathy was considered an early stage of membranous glomerulonephropathy. 相似文献
108.
109.
Shogo Matsumoto Yui Furusawa Hiromitsu Komatsu Junichi Soejima 《The Journal of Horticultural Science and Biotechnology》2013,88(5):634-637
SummaryWe examined S-allele genotypes of ten apple cultivars and species to determine their possible usefulness as pollenizers for all apple cultivars. ‘Dolgo’ did not contain any known S-RNases encoded at the S-locus, suggesting its possible usefulness as a pollenizer for almost all apple cultivars. We also identified and confirmed the S-allele genotypes of 18 apple cultivars by polymerase chain reaction (PCR)-digestion analysis. The S-genotype of ‘Kiou’ (S1S7), ‘Korei’ (S3S28), ‘Korin’ (S1S9), ‘Kotoku’ (S1S28), ‘Kyokkou (S7S25), ‘Lobo’ (S1S7), ’Mahe 7’ (S2S7), ‘Mellow’ (S2S3), ‘Takahara’ (S3S9) and ‘Warabi’ (S9S28) were confirmed by pollination results. These cultivars seemed not to have originated from the expected seed or pollen parents or, in the case of ‘Lobo’, might have been mislabelled. Finally, we identified the S-allele genotypes of ‘Prima’ (S2S10), ‘Querina’ (S3S9) and ‘Yoko’ × ‘Prima’ (S3S10), which are resistant to scab. 相似文献
110.
We exposed Akoya cultured pearls separately to heat (60–120 °C) and artificial light to investigate changes to fluorescence in the visible range and yellowing. We found that for both heat-treated and light-treated pearls, the fluorescence peak shifted from 480 to 430 nm with an increase in fluorescence intensity. This change in intensity was more prominent in heat-treated pearls, with the initial speed of increase rising with treatment temperature; treatment at 100 °C caused the greatest increase in fluorescence intensity. However, aminoguanidine suppressed the heat-induced change in the fluorescence of an ethylenediaminetetraacetic acid–soluble nacreous layer matrix. These results suggest that the heated-induced changes in the fluorescence of Akoya cultured pearls were caused largely by a buildup of fluorescent advanced glycation end products through the Maillard reaction. Although heat treatment led to a large increase in fluorescence intensity of the peak at approximately 430 nm in a deoxygenized environment, hardly any change in fluorescence intensity was observed after light treatment in this environment. Moreover, a new shoulder peak appeared at about 460 nm after light treatment. These results suggest that the Maillard reaction was not a major factor in the light-induced changes in the fluorescence of Akoya cultured pearls. 相似文献