利用游离小孢子培养育成早熟大白菜新品种‘豫新5号’

 ‘豫新5号’是采用游离小孢子培养双单倍体育种技术选育的一代杂种，该品种生育期60 d，单球质量23 kg，球形指数1.35，高抗病毒病、霜霉病和软腐病，净菜产量56.7 t·hm^-2，可兼作早熟、晚熟栽培。     

Selection and amplification of the liver stem cell subset from rat bone marrow cells with a medium containing cholestatic serum in vitro

AIM: To explore the feasibility of direct separat and selective enlargement of the bone marrow-derived liver stem cells (BDLSC) from bone marrow cells with a culture system containing cholestatic serum in vitro. METHODS: Bone marrow cells of rats were cultured with selective media containing 2%, 5%, 7% and 10% cholestatic rat serum, respectively. The BDLSC were then induced to proliferate with the addition of hepatocyte growth factor (HGF) on the firth day. BDLSC were characterized using immunocytochemistry and RT-PCR for lineage markers, glycogen staining and urea synthetic assay for functions 2 weeks later. RESULTS: Bone marrow cells were unble to form colony in the presence of 2% cholestatic serum and apopotosis appeared gradually in 7% or 10% cholestatic serum. The BDLSC survived in the medium containing 5% cholestatic serum while the other types of cells did not. The survival cells proliferated with a high speed during the second week and then formed hepatocyte-like colony-forming units (H-CFU). Cells in the H-CFU expressed the characteristic proteins of fetal hepatocytes. Furthermore, they had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: The selective micro-environment effectively selected BDLSC from the bone marrow cell, and will be a new way to provide an abundant source of donor hepatocytes for clinical cell therapy.     

Effect of mutant β-catenin gene on the proliferation of hepatocytes

AIM:The β-catenin is a key molecule in the Wnt signal pathway, which plays a critical role in normal development and tumorigenesis. However, the mechanisms of the β-catenin on the cell growth control are still not completely defined. The aim of this study was to test the hypothesis that the mutant β-catenin may regulate the hepatocyte proliferation. METHODS: The immortalized murine hepatocyte cell line, AML12, was used for this study. A plasmid that contain mutant β-catenin S33Y was transfected into the AML12 cells and a stable cell line AML12S33Y was established. The cell growth property of this cell line and the parental cell were compared by flow cytometry analysis and direct cell count. The cells were also tested for the ability to form soft agar colonies, and the ability to form tumors in the severe immune deficient mice (SCID). RESULTS:1. The mutant β-catenin containing cell line AML12S33Y has higher proliferating index compared with the parental AML12 cells (P<0.01), suggesting that mutant β-catenin promotes cell growth. 2. The mutant β-catenin cells formed small colonies in soft agar after 4 weeks of culture, but did not generate tumor in SCID mice. CONCLUSION:The mutant β-catenin promotes liver cell growth.     

区域旅游资源开发定位研究——以内蒙古赤峰市为例

区域旅游资源开发是发展区域旅游的基础 ,开发的定位研究有助于为一地旅游业的发展指明方向。本文从区域角度和空间经济一体化思想出发 ,以赤峰市为例 ,通过对旅游资源的定量与定性研究 ,得到旅游资源的综合评价图式 ,结合区际分析归纳出赤峰市旅游资源开发利用的方向和层次。从而为区域旅游开发的定位研究进行了一般理论的探讨及例证分析     

几种除草剂药液表面张力、叶面接触角与药效的相关性研究

在除草剂氟磺胺草醚、灭草松和精喹禾灵药液中添加不同浓度的喷雾助剂ABS(十二烷基苯磺酸钠)和JFC ,以温室盆栽法测得各处理抑制杂草的效果,结果表明,在多数情况下添加喷雾助剂可使3种供试除草剂的药效显著提高。     

茭白纹枯病发生规律及产量损失测定

茭白纹枯病发生规律与产量损失的研究结果表明:高温高湿、种植过密、偏施氮肥、缺乏钾素是病害加重的主要原因;田间发病盛期在7月中旬至8月中旬;单茭重减低率与病级之间呈极显著线性相关;始病期越早,危害越重,产量损失也越大;病害危害程度与有效分蘖数(孕茭数)减少率、单茭重减低率、产量损失率之间呈高度正相关。在产量损失构成中,有效分蘖数的减少为主要因素。     

晚稻稻瘟病BP神经网络分区预报

应用相关分析方法分析了浙江省19个县1988～1999年晚稻稻瘟病发病与有关环境因子的关系,筛选了8个气象因子用于晚稻稻瘟病发生程度长期预报。根据各预报因子与稻瘟病发病程度相关性,采用邻接二维图论聚类分析法,将19个点(县)划分为4个生态区。每个生态区内运用BP神经网络技术建立模型,并进行拟合和试报。1997～1999年试报验证,在划分稻瘟病生态区的基础上,应用BP神经网络模型对稻瘟病进行长期预测预报是可行的,3年试报成功率分别是78.95％、84.21％和78.95％。文中还对该方法与过去常用的预报方法的试报结果作了比较。     

转Bt基因棉在安庆市大面积种植后害虫发生特点调查

根据调查,转Bt基因棉大面积种植后,棉铃虫、红铃虫发生减轻;棉叶螨发生加重,为害时间延长;甜菜夜蛾、斜纹夜蛾发生面积扩大,为害程度加重。据此初步提出了当地转Bt基因棉害虫防治中应注意的问题和管理对策。     

巴旦杏在甘肃河西地区引种培育问题的探讨

该文通过分析巴旦杏在甘肃河西地区引种培育过程中出现的问题,总结出用容器育苗和大田开沟点播相结合来培育接穗,以山桃为砧木嫁接巴旦杏的引种驯化技术.结果表明,容器培育接穗比直接大田点播出苗率、成活率分别提高了10%和15%;巴旦杏与山桃嫁接表现出极好的亲和性,嫁接成活率为90%.     

沙棘植物资源的综合利用

依据有关沙棘的研究文献,综述了沙棘的形态特征、生物学特征与分布及其生态效益、生化特性、医学用途、饮料、食品、化妆品生产中的应用、饲用价值等,提出了沙棘资源当前与今后开发利用的建议.