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61.
Sserumaga Julius Pyton Kayondo Siraj Ismail Kigozi Abasi Kiggundu Muhammad Namazzi Clementine Walusimbi Kato Bugeza James Molly Allen Mugerwa Swidiq 《Genetic Resources and Crop Evolution》2021,68(7):2997-3010
Genetic Resources and Crop Evolution - Most orphan crops have not been fully sequenced, hence we rely on genome sequences of related species to align markers to different chromosomes. This hinders... 相似文献
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Hani A. Alhadrami Bathini Thissera Marwa H. A. Hassan Fathy A. Behery Che Julius Ngwa Hossam M. Hassan Gabriele Pradel Usama Ramadan Abdelmohsen Mostafa E. Rateb 《Marine drugs》2021,19(2)
Coculture is a productive technique to trigger microbes’ biosynthetic capacity by mimicking the natural habitats’ features principally by competition for food and space and interspecies cross-talks. Mixed cultivation of two Red Sea-derived actinobacteria, Actinokineospora spheciospongiae strain EG49 and Rhodococcus sp. UR59, resulted in the induction of several non-traced metabolites in their axenic cultures, which were detected using LC–HRMS metabolomics analysis. Antimalarial guided isolation of the cocultured fermentation led to the isolation of the angucyclines actinosporins E (1), H (2), G (3), tetragulol (5) and the anthraquinone capillasterquinone B (6), which were not reported under axenic conditions. Interestingly, actinosporins were previously induced when the axenic culture of the Actinokineospora spheciospongiae strain EG49 was treated with signalling molecule N-acetyl-d-glucosamine (GluNAc); this finding confirmed the effectiveness of coculture in the discovery of microbial metabolites yet to be discovered in the axenic fermentation with the potential that could be comparable to adding chemical signalling molecules in the fermentation flask. The isolated angucycline and anthraquinone compounds exhibited in vitro antimalarial activity and good biding affinity against lysyl-tRNA synthetase (PfKRS1), highlighting their potential developability as new antimalarial structural motif. 相似文献
64.
Julius J. Okello Carl Johan Lagerkvist Penina Muoki Simon Heck Gordon Prain 《Journal Of Agricultural & Food Information》2013,14(3):237-254
This study used a field experiment and the means-end chain approach to examine the effect of providing information on the biofortification process on consumers' acceptance of orange-fleshed sweetpotato (OFSP). Negative, positive, and neutral information about the biofortification were verbally provided. Images were used to reinforce the verbal information. Meaning representation (mental models) of various constructs relating to OFSP was assessed. We found that the structure of mental constructs differed depending on the type of information provided and concluded that the type of information consumers receive about the biofortification process affects OFSP acceptance. Implications of the findings for policy and development practice are discussed. 相似文献
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CASE DESCRIPTION: A 2-year-old Siberian Husky was evaluated because of a 2-week history of coughing and gagging and decreased appetite and activity level. CLINICAL FINDINGS: Radiography, surgery, and immunohistochemical examination revealed a solitary sclerosing mesothelioma extending from the left thoracic diaphragmatic surface that was adherent to the pericardium and the caudal mediastinum. TREATMENT AND OUTCOME: The tumor was resected along with most of the left hemidiaphragm, and the left transversus abdominis muscle was used to reconstruct the diaphragm. The 13th rib formed the base of the muscle flap. The muscle flap was transposed into the defect so that the mesothelium-lined surface faced the thoracic cavity and the deep aspect of the muscle formed the abdominal surface of the diaphragm. To minimize risk of adhesions, the exposed raw aspect of the abdominal surface was covered with porcine small intestinal submucosa. Recovery was uncomplicated, and the dog's appetite and activity level soon returned to normal. Evaluation 54 days after surgery revealed 2 subcutaneous masses on the thorax and masses in the liver and both kidneys; histologic and immunohistochemical analyses revealed metastasis of the original tumor. The dog was euthanatized. CLINICAL RELEVANCE: Hemidiaphragmatic reconstruction with a transversus abdominis muscle flap after resection of a diaphragmatic tumor was successful. The muscle flap was easily harvested and transposed into the diaphragmatic defect. 相似文献
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Nyangaga Julius N. Grace Delia Kimani Violet Kiragu Monica W. Langat Alfred K. Mbugua Gabriel Mitoko Grace Kangethe Erastus K. 《Tropical animal health and production》2012,44(1):47-51
A study was undertaken to investigate and mitigate the risk from zoonotic Cryptosporidium associated with dairy farming in Dagoretti division, Nairobi, Kenya. Outcome mapping (OM), a relatively new tool for planning and evaluation, was used to foster and then monitor changes in farmer management of health risks. Elements of the OM framework, including the vision, mission and expected progress markers, were developed in participatory sessions and a set of progress markers was used for monitoring behaviour change in farmers participating in the project (the boundary partners). Behaviour change (the outcome challenge) was supported by a range of awareness and educational campaigns, working with strategic partners (extension agents and administrative leaders). The farmers the project worked with made considerable progress according to the markers; they demonstrated an understanding of cryptosporidiosis, established or maintained clean and well drained cattle sheds, and took conscious effort to reduce possible infection. Farmers who did not participate in the project (non-contact farmers) were found to be less advanced on the progress marker indicators. Non-contact farmers who carried out risk-reducing practices had done so independently of the project team. The administration leaders, as strategic partners, had a positive attitude towards the project and confidence in their ability to support project objectives. The study demonstrates the utility of OM in helping to identify and support behavioural change. 相似文献
68.
Oluwatoyosi Oyetola Oyebiyi Julius Olayinka Ojetade Sikiru Adekoya Muda Adeagbo Alani Amusan 《Communications in Soil Science and Plant Analysis》2018,49(18):2338-2344
The cation exchange capacity (CEC) of three benchmark soils in the rain forest region of Southwestern Nigeria, were measured by three standard CEC methods. Results obtained were compared with a view to selecting the best suited CEC method for the soils. The study sites were the Teaching and Research Farm of Obafemi Awolowo University, Ile-Ife, and Itagunmodi settlement. Two representative soil profile pits each were established in soils developed in coarse-grained granite and gneiss, fine-grained biotite gneiss and schist, and the amphibolite. The three methods gave different CEC values in the order of CEC-pH 8.2 > CEC-pH 7 > ECEC. There was a significant correlation between soil organic matter (SOM) and the CEC obtained by each of the three methods (P ≤ 0.05), meanwhile, total clay showed no significant correlation. ECEC provided the best estimate of the CEC for the soils and adequate SOM management is crucial to enhance sustainable productivity of the soils. 相似文献
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Liptak JM Dernell WS Ehrhart N Lafferty MH Monteith GJ Withrow SJ 《Veterinary surgery : VS》2006,35(6):518-533
OBJECTIVE: To compare surgical and oncologic outcome in dogs with osteosarcoma (OSA) of the distal aspect of the radius treated with limb-sparing surgery, using either a cortical allograft or endoprosthesis, and postoperative chemotherapy; and to evaluate predictive factors for outcome. STUDY DESIGN: Prospective cohort study. ANIMALS: Dogs (n = 20) with spontaneous, non-metastatic OSA of the distal aspect of the radius. METHODS: Dogs were prospectively randomized for limb-sparing surgery with either a cortical allograft (n = 10) or endoprosthesis (10) and full-course adjuvant chemotherapy using single or dual agent protocols of cisplatin, carboplatin, and/or doxorubicin. Surgical (intraoperative findings, postoperative infection, construct failure) and oncologic (local tumor recurrence, metastasis, survival) outcomes were compared. The influence of intraoperative and postoperative variables on surgical and oncologic outcome were evaluated. RESULTS: No clinically significant differences in surgical and oncologic outcome were detected between groups. The percentage of radius replaced by the implant was significantly greater in the endoprosthesis group (60.9% compared with 48.6%, P = .008). Median survival time (MST) for dogs with construct failure, regardless of implant type, was 685 days and significantly greater than MST of dogs without construct failure (322 days, P = .042; hazard ratio [HR] 16.82). Median metastasis-free interval and MST (685 days versus 289 days; P = .034, HR 24.58) were significantly greater in dogs with postoperative infection. Disease-free and overall limb-salvage rates were 70% and 85%, respectively. Overall MST was 430 days. CONCLUSIONS: For dogs with OSA of the distal aspect of the radius, a cortical allograft or endoprosthesis can be used for limb-sparing surgery. Construct failure and postoperative infection significantly improve survival time regardless of implant type. CLINICAL RELEVANCE: An endoprosthesis is an attractive alternative to cortical allografts for limb-salvage of the distal aspect of the radius in dogs because surgical and oncologic outcomes are similar, but the endoprosthesis is an immediately available off-the-shelf implant which is not complicated by the bone harvesting and banking requirements associated with cortical allografts. Mechanisms whereby postoperative infection improves survival time requires further investigation and, if elucidated, may provide the opportunity to improve the outcome of dogs and humans with OSA. 相似文献