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961.
Tricaine as an anaesthetic for larvae and juveniles of Lophiosilurus alexandri,a carnivorous freshwater fish 下载免费PDF全文
962.
Pérez-Prior MT Manso JA García-Santos Mdel P Calle E Casado J 《Journal of agricultural and food chemistry》2005,53(26):10244-10247
A kinetic study of the alkylating potential of potassium sorbate (S)-a food preservative used worldwide-in 7:3 water/dioxane medium was performed. The following conclusions were drawn: (i) Potassium sorbate shows alkylating activity on the nucleophile 4-(p-nitrobenzyl)pyridine (NBP), a trap for alkylating agents with nucleophilic characteristics similar to those of DNA bases, (ii) The NBP alkylation reaction complies with the rate equation r = k(alk)[H+][S][NBP]/(K(a) + [H+]), K(a) being the sorbic acid dissociation constant and k(alk) the rate constant of NBP alkylation by the undissociated acid. In the range of pH 5-6, the alkylation time ranges between 18 days (pH 5.2) and >1 month (pH > or = 6). (iii) NBP alkylation occurs through a reaction with deltaH# = 78 kJ mol(-1), which is much higher than those of NBP alkylation by stronger alkylating agents. (iv) The absorption coefficient of the sorbate-NBP adduct was determined to be epsilon = 204 M(-1) cm(-1) (lambda = 580 nm), this value being rationalized in terms of the adduct structure. (v) The results can help to establish suitable expiration times for products preserved with potassium sorbate. 相似文献
963.
Tudela JA Hernández JA Gil MI Espín JC 《Journal of agricultural and food chemistry》2003,51(15):4296-4302
L-galactono-gamma-lactone dehydrogenase (GLDH) activity and vitamin C content as ascorbic acid (AA) plus dehydroascorbic acid (DHA) were evaluated in five potato tuber cultivars (Agata, Altesse, Franceline, Manon, and Monalisa). The effect of fresh-cutting and subsequent refrigerated storage of Manon potato under different atmospheres (air, 20% CO(2) + air, 100% N(2), and vacuum packaging) on GLDH activity and vitamin C content was also determined. GLDH from the five potato tuber cultivars showed typical inhibition kinetics by high substrate concentration in the synthesis of AA from its physiological precursor L-galactonic acid-gamma-lactone (GL). GLDH activity was not correlated with the corresponding vitamin C content in any potato tuber cultivar. GLDH from all the cultivars presented a major isoform with isoelectric point (IEP) 5, which changed to IEP = 4.3 after minimal processing. In addition, the GLDH-catalyzed synthesis of AA by the new isoform showed typical Michaelis kinetics, in which the enzyme became more efficient to catalyze the reaction. Whether the change in the isoform pattern was due to either post-translational modifications or de novo synthesis of a new isoenzyme remains unanswered. Fresh-cutting increased GLDH activity from 4.7-fold (vacuum packaging) to 11-fold (air) after 6 days. In addition, 100% of vitamin C content was retained in air and decreased in the rest of atmospheres after this storage period, following the sequence vacuum packaging (89%) > 100% N(2) (78%) > 20% CO(2) + air (63%). This tendency was correlated with the corresponding GLDH activity detected in each storage atmosphere, except in the case of 20% CO(2) + air. Vacuum packaging proved to be the best storage condition, because fresh-cut potatoes did not turn brown and retained 89% of initial vitamin C content. 相似文献
964.
Kuskoski EM Vega JM Rios JJ Fett R Troncoso AM Asuero AG 《Journal of agricultural and food chemistry》2003,51(18):5450-5454
Anthocyanin pigments in the berries of bagua?u (Eugenia umbelliflora Berg), a tropical fruit from Brazil, were extracted with 0.1% HCl in ethanol, and the crude anthocyanin extract was purified by Amberlite XAD-7 open-column chromatography. Six major anthocyanins were isolated by preparative HPLC, and their chemical structures were identified by spectroscopic methods (TLC, UV-vis, MS, and (1)H NMR). Delphinidin 3-O-beta-glucopyranoside, cyanidin 3-O-beta-glucopyranoside, petunidin 3-O-beta-glucopyranoside, pelargonidin 3-O-beta-glucopyranoside, peonidin 3-O-beta-glucopyranoside, and malvidin 3-O-beta-glucopyranoside were identified. On the basis of chromatographic data the total anthocyanin content was 342 mg/100 g of fresh bagua?u berries. Therefore, the concomitant presence of six anthocyanins in a single plant species makes this product promising as a new pigment source. 相似文献
965.
Pionnier E Chabanet C Mioche L Taylor AJ Le Quéré JL Salles C 《Journal of agricultural and food chemistry》2004,52(3):565-571
This study deals with the release kinetics of nonvolatile compounds (NVC) (leucine, phenylalanine, glutamic acid, citric acid, lactic acid, propanoic acid, sodium, potassium, calcium, magnesium, chloride, and phosphates) during the eating of a model cheese and the relationships to some oral (salivary and masticatory) parameters. The aroma release has previously been characterized in similar conditions [Pionnier, E.; Chabanet, C.; Mioche, L.; Le Quéré, J.-L.; Salles, C. J. Agric. Food Chem. 2004, 52, xxx-xxx (1)]. Saliva samples were collected from the tongues of eight assessors at different times during and after the chewing sequence. Atmospheric pressure ionization-mass spectrometry and/or high-performance liquid chromatography analyses have been performed on these samples in order to quantify the 12 NVC released in saliva. The maximum concentration (C(max)) in saliva varied significantly according to the compound. However, there was no significant effect of the compound on the time to reach maximum concentration (T(max)). Interindividual differences were observed for most of the parameters and for all of the NVC studied. The parameters extracted from the release profiles of the NVC were closely correlated. High T(max) and AUC (area under the curve) values could be related to high chewing time and low saliva flow rates, low chewing rates, low masticatory performances, and low swallowing rates. 相似文献
966.
Perales S Barberá R Lagarda MJ Farré R 《Journal of agricultural and food chemistry》2006,54(13):4901-4906
Calcium solubility, dialysability, and transport and uptake (retention + transport) by Caco-2 cells as indicators of calcium bioavailability have been estimated in the in vitro gastrointestinal digests of milk and calcium fortified milk. A significant linear correlation (p < 0.05) was obtained between calcium uptake and the amount of soluble calcium added to the cells, and also between percentage calcium uptake and the calcium measured in the analyzed samples. The solubility, dialysis, transport, and uptake values are higher (p < 0.05) for calcium fortified milks than for nonfortified milks; that is, calcium fortification increases not only calcium content but also its bioavailability. An inhibitory effect of calcium from fortified milks upon iron absorption was found. The observed effect of calcium from fortified milks upon zinc bioavailability depends on the in vitro method used, zinc solubility and dialysis decrease in calcium fortified milks, and percentage zinc uptake remains unchanged. 相似文献
967.
von Bernuth H Picard C Jin Z Pankla R Xiao H Ku CL Chrabieh M Mustapha IB Ghandil P Camcioglu Y Vasconcelos J Sirvent N Guedes M Vitor AB Herrero-Mata MJ Aróstegui JI Rodrigo C Alsina L Ruiz-Ortiz E Juan M Fortuny C Yagüe J Antón J Pascal M Chang HH Janniere L Rose Y Garty BZ Chapel H Issekutz A Maródi L Rodriguez-Gallego C Banchereau J Abel L Li X Chaussabel D Puel A Casanova JL 《Science (New York, N.Y.)》2008,321(5889):691-696
MyD88 is a key downstream adapter for most Toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs). MyD88 deficiency in mice leads to susceptibility to a broad range of pathogens in experimental settings of infection. We describe a distinct situation in a natural setting of human infection. Nine children with autosomal recessive MyD88 deficiency suffered from life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease. However, these patients were otherwise healthy, with normal resistance to other microbes. Their clinical status improved with age, but not due to any cellular leakiness in MyD88 deficiency. The MyD88-dependent TLRs and IL-1Rs are therefore essential for protective immunity to a small number of pyogenic bacteria, but redundant for host defense to most natural infections. 相似文献
968.
Márcia Rufini Michele Aparecida Pereira da Silva Paulo Ademar Avelar Ferreira Alice de Souza Cassetari Bruno Lima Soares Messias José Bastos de Andrade Fatima Maria de Souza Moreira 《Biology and Fertility of Soils》2014,50(1):115-122
Cowpea (Vigna unguiculata (L.) Walp.) is one of the most important crops in the north and northeast regions of Brazil, where it is grown under the subsistence regime, mainly because of its adaptation to the local edaphoclimatic conditions. This species is an important legume in Brazil because of its significant nutritional value in the diet of low-income populations. Moreover, it is able to benefit from the process of biological nitrogen fixation, which leads to a significant reduction in production costs. The objective of this research was to evaluate the agronomic efficiency of bacterial strains approved by the Ministry of Agriculture, Livestock and Supply for commercial inoculant production when in symbiosis with cowpea in a Rhodic Eutrudox in Lavras, MG, as well as to identify and verify the efficiency of the native populations that nodulate cowpea. Agronomic efficiency of nitrogen-fixing nodulating bacterial strains (UFLA 03-84, INPA 03-11B, and BR 3267) in symbiosis with cowpea was evaluated in the field. Subsequently, the efficiency and diversity of isolates from the nodules obtained in the field were evaluated under greenhouse conditions. The genetic diversity of the efficient isolates was determined by partial sequencing of the 16S rRNA gene. Grain yields obtained by the inoculation of seeds in the field with the strains were similar to those of the controls without inoculation with and without mineral N addition indicating the existence of efficient native populations. In the native community, we identified isolates with similar or higher efficiency than the strains approved for cowpea inoculation and the control that received mineral N. The isolates subjected to partial sequencing of the 16S rRNA gene were phylogenetically related to the Bradyrhizobium genus. 相似文献
969.
Bruno Araujo Furtado de Mendonça Elpídio Inácio Fernandes Filho Carlos Ernesto Gonçalves Reynaud Schaefer Anôr Fiorini de Carvalho José Frutuoso do Vale Jr. Guilherme Resende Corrêa 《Journal of Soils and Sediments》2014,14(3):525-537
Purpose
The vegetation of the Campinaranas occurs in humid areas with hydromorphic sandy soils at the Amazon region. Thus, the determination and in situ monitoring of moisture content in Campinarana soils, besides the detection of subsurface layers are key measures for studying these soil–vegetation systems. Also, the application of ground penetrating radar (GPR) in deep sandy sedimentary sequence of Amazonia is a promising tool to enhance the knowledge on depositional and soil formation features.Materials and methods
We studied representative soils of the Campinaranas at the National Park of Viruá, state of Roraima (Brazilian Amazonia), through the use of geophysical methods (soil moisture sensors and GPR). The study was applied in four sandy soils. Besides chemical and physical analysis of soils, soil moisture sensors were installed for monitoring during an entire hydrological year (2010/2011), and performed the calibration of sensors , coupled with imaging of the soil along transects with GPR.Results and discussion
As a result of calibration of the soil moisture sensors we obtained a general equation with an R 2 greater than 0.9. There is an influence of soil moisture content and soil temperature in the distribution of vegetation types in Campinaranas. The use of GPR identified some determinants characteristics in these soils for the differentiating the Campinaranas, represented by spodic and C horizons.Conclusions
The spodic horizons in soils under Forest Campinarana provided potential errors in the determination of soil moisture, requiring calibration data for the precise use of this device. The investigation of the soil through the GPR showed interesting results, which allowed continuous visualization of the main soil horizons along transects in the phytophysiognomies of Campinaranas. 相似文献970.
Judite S. Vieira José C. M. Pires Fernando G. Martins Vítor J. P. Vilar Rui A. R. Boaventura Cidália M. S. Botelho 《Water, air, and soil pollution》2012,223(9):5549-5561
This study presents the application of multivariate statistical tools for the evaluation of spatial variations and the interpretation of water quality data obtained in a monitoring program of Lis river basin surface water, Portugal. Twenty-seven physicochemical and microbiological parameters were determined in six water sampling campaigns at 16 monitoring sites during the period from September 2003 to November 2006. Correlation analysis, principal component analysis, and cluster analysis were performed to evaluate the main water pollution sources and to characterize the spatial distribution of water pollution profiles in river basin. The results achieved with the statistical methodologies led to distinguish natural and anthropogenic pollution sources. Additionally, monitoring sites with similar water pollution profile were identified, indicating that some monitoring locations can be changed to improve the spatial characterization of water quality in the river basin. CBO, CQO, P, and N were identified as significant variables affecting spatial variations, namely in the Lis river middle reach. Besides the identification of main pollution sources, the applied statistical tools were able to identify spatial patterns of water pollution in Lis river basin, which further helps in the reassessment of the number and location of monitoring sites. 相似文献