Feline hip joint anatomy in magnetic resonance images

The aim of this study was to develop an anatomical model of the feline hip joint for low‐field magnetic resonance imaging (LF‐MRI) based on high‐field magnetic resonance imaging (HF‐MRI). The study was performed on six adult clinically healthy European shorthair cats, aged 1–3 years, with body weight of 2.8–4.4 kg. The animals were examined with the use of the Vet‐MRI Grande Esaote LF (0.25 T) scanner and high‐field Siemens Magnetom TRIO (3 T) MRI scanner. In the LF‐MRI, most satisfactory results in T1‐weighted images were obtained when TE was 26 ms in all three planes and when TR was 350–950 ms in the transverse plane, 950–1150 ms in the sagittal plane and 520–750 ms in the dorsal plane. In T2‐weighted images, TE was 90 ms in the transverse and dorsal plane and 120 ms in the sagittal plane. The results were presented as images acquired with LF‐MRI scanners in three planes. The slice thickness was 3 mm for each plane. In LF‐MRI, muscles in the hip joint region and round ligament were well visualized. Unlike in LF‐MRI, the cross section of the femoral nerve was identified in HF‐MRI scans. In examinations of the feline hip joint, the main limitations of LF‐MRI were a lack of reliable contrast between articular cartilage and synovial fluid as well as longer scan time. Despite the above, LF‐MRI images were characterized by good contrast between bones and the surrounding soft tissues.     

Functional response of <Emphasis Type="Italic">Quercus robur</Emphasis> L. to taproot pruning: a 5-year case study

Key message

Quercus robur seedling mass was affected more by planting density than by taproot pruning. Root pruning enhanced stem biomass at the expense of roots in later growth stages. Alteration of biomass allocation due to nursery practices may result in greater susceptibility to injury and death of the seedlings under unfavorable environmental conditions.

Context

Plants adjust their growth and modulate the resource allocation in response to applied treatments and environmental conditions.

Aims

The aim was to examine how taproot pruning in seedlings grown at different densities affected long-term growth of Quercus robur.

Methods

Seedlings, sown as acorns at two planting densities, with or without pruned roots were harvested in the second, fourth, and fifth years of growth. The effect of root pruning on biomass allocation was determined by measuring leaf, stem, and root mass fractions; carbohydrate concentrations in the roots; and C/N ratios. Specific leaf area and root length were also determined to assess morphological adaptations to growth conditions.

Results

Total seedling mass was affected more by planting density than by taproot pruning. After 4 years of growth, root mass fractions were lower and stem mass fractions were greater in seedlings planted at a higher density. Five-year old root-pruned seedlings also had a lower root mass fraction and higher stem mass fractions than unpruned seedlings. Specific root length was not affected by root pruning or planting density.

Conclusion

Decrease of relative root biomass with simultaneous increase of stem biomass may be a long-term consequence of taproot pruning of Q. robur, and the effects may manifest years after the seedling stage.

     

Application of reactive UV-absorbers for increasing protective properties of cellulose fabrics during standard laundering process

A number of water-soluble colourless fibre-reactive UV-absorbers derivatives of symmetrical triazine were used as additives during commercial laundering process. UV-protection factor (UPF) of such treated cotton tricot samples was measured. Changes in protection properties of modified textiles after repeated standard laundering process were determined. Carried-out experiments gave evidence of covalent bond formation between applied absorbers and cellulose fibre during standard washing process at 40 °C or 60 °C. Textiles modified by this method have provided UV-protective properties for prolonged time.     

Cut-flower life of dry transported carnations as influenced by different silver form pre-treatments

Twenty-four hours of pre-treatment with AgNO₃ increased the vase-life of flowers from 3 to 4 days for carnations kept in water or in preservative solutions after simulated transport conditions. A mixture of AgNO₃ with sodium thiosulphate or with EDTA was considerably better than AgNO₃, while such complexing agents as CDTA, EDDHA and EDPA were less effective. Determination of radioactive silver (Ag) in dissected carnation parts treated with different Ag complexes showed a positive correlation between vase-life and the amount of tracer Ag in the plants.     

Graduate students' teaching experiences improve their methodological research skills

Science, technology, engineering, and mathematics (STEM) graduate students are often encouraged to maximize their engagement with supervised research and minimize teaching obligations. However, the process of teaching students engaged in inquiry provides practice in the application of important research skills. Using a performance rubric, we compared the quality of methodological skills demonstrated in written research proposals for two groups of early career graduate students (those with both teaching and research responsibilities and those with only research responsibilities) at the beginning and end of an academic year. After statistically controlling for preexisting differences between groups, students who both taught and conducted research demonstrate significantly greater improvement in their abilities to generate testable hypotheses and design valid experiments. These results indicate that teaching experience can contribute substantially to the improvement of essential research skills.     

Tissue-specific responses of oxidative stress biomarkers and antioxidant defenses in rainbow trout Oncorhynchus mykiss during a vaccination against furunculosis

The present study was conducted to evaluate the effects of vaccination against furunculosis on responses of oxidative stress and antioxidant defenses in rainbow trout Oncorhynchus mykiss muscle, gills, liver, and brain tissues. The oxidative stress markers (malondialdehyde and carbonyl derivatives of protein oxidative destruction levels), antioxidant defenses (superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase), and total antioxidant capacity in different tissues of rainbow trout were measured. Our data showed that exposure of trout to vaccine against furunculosis produced changes (either increase or decrease) in oxidative stress and antioxidant enzymes responses, and these responses showed marked organ differences, associated with tissue patterns. Our study demonstrated that vaccinated trout showed alteration in antioxidant defenses and oxidative stress responses, with higher severity in the liver, compared with other tissues. Our data also suggest that vaccination against furunculosis induced lipid peroxidation in gill and liver tissues. However, muscle and brain tissue are capable of restoring its pro- and antioxidant balance after vaccination.     

Effect of temperature on growth and survival of maraena whitefish Coregonus maraena (Bloch 1779) larvae in controlled conditions

This 28‐day study investigated the effect of three rearing temperatures, 11, 15 and 19°C, on survival and growth of maraena whitefish fry in a recirculating aquaculture system. Three groups of larvae in three repetitions were reared in recirculating system. Each group comprised 200 larvae. Feeding level was fixed at 500–700 Artemia sp. metanauplii per fish per day. Larvae were fed fresh live brine shrimp at 10 ml/tank every 3 hr. Significantly higher body weight (p = 0.00), total length (p = 0.00), larval yield (p = 0.00) and condition factor (p = 0.00) were obtained at 19°C compared to 15 and 11°C, as well as at 15°C compared to 11°C. Significantly higher survival (p = 0.00) was observed in larvae reared at 11 and 15°C compare to 19°C and no significant differences were observed between 11°C compared to 15°C. No significant differences in size heterogeneity among treatments were found (p = 0.46). In larviculture, the optimal assessed temperature for growth of maraena whitefish was 19°C, with highest survival observed at 11°C, at the end of this 28 days trial. The findings in this study apply to the particular study location and may not be applicable more broadly.