Uterine Expression of WNT7A and β-catenin After Induction of Oestrus in Sheep

WNT7A and β-catenin localisations and roles in regulating periimplantation ovine conceptus development under natural estrous conditions have been elaborated.However,their locations and expression patterns have not been reported under induction of oestrus.The localisation,expression and function of WNT7A and β-catenin in the uterine tissues of the early pregnant and non-pregnant sheep on days 10,12,14,16 and 18 following artificial induction of oestrus were investigated by means of in situ hybridisation,real-time RT-PCR,immuno-histochemistry and western blotting methods.WNT7A and β-catenin mRNA and protein were both restricted to the apical surfaces of the uterine luminal epithelium (LE) and glandular epithelium (GE).In pregnant sheep,protein localisation of WNT7A and β-catenin was observed both in the endometrial LE and GE.Their staining presented on day 10,increased between day 12 and day 16,and decreased on day 18.WNT7A and β-catenin mRNA and protein expression increased initially and then decreased from day 10 to day 18,peaking on day 16,and β-catenin reaching a peak on day 18 in the uterine tissues of pregnant sheep (p0.05).By contrast,no significant changes in WNT7A and β-catenin mRNA and protein expression levels were observed from day 10 to day 18 of the oestrus cycle in the uterine tissues of non-pregnant sheep (p0.05).Additionally,WNT7A and β-catenin mRNA and protein expression levels in the uterine tissues of the early pregnant sheep were significantly higher than those of non-pregnant sheep (p0.05).Treatment of endometrial epithelial cells with WNT7A increased the mRNA expressions of β-catenin,c-myc and Cyclin D1.These results provided an underlying mechanism of periimplantation ovine conceptus development under induction of oestrus.     

农药使用与无公害农产品生产

农药的使用是无公害农产品生产的重要环节.正确认识农药的毒性特点及农药残留,了解无公害农产品生产对农药使用的要求,做到科学合理,是无公害农产品生产健康稳定发展的保证,也是必须解决的问题.     

2018年我国农药进出口特点与2019年趋势分析

Li Lin;Jin Hejiang;Huang Xinhe;Cao Bingwei;Wu Lihui;Liu Pingping;Qu Mengmeng;Wu Houbin(The Institute for the Control of Agrochemicals,Ministry of Agriculture and Rural Affairs,Beijing 100125,China)     

作物地膜覆盖技术适宜性及其在东北春玉米上的应用

为从源头解决地膜覆盖技术泛用、滥用问题,提高作物地膜覆盖技术的合理利用性。研究提出作物地膜覆盖适宜性的概念,将其定义为"地膜覆盖技术对作物自身环境要素需求与所在地提供环境要素差异的补偿程度"。该研究以东北春玉米为例,构建春玉米地膜覆盖适宜性的评价体系,通过数据检索的方式收集东北地区田间试验中春玉米地膜覆盖功能数据,明确作物地膜覆盖与不覆盖农田土壤温度和水分、经济产量相关关系,量化作物地膜覆盖的增温保墒、增产功效,筛选春玉米地膜覆盖技术适宜性评价指标,并计算地膜覆盖技术适宜指数,参照作物需求阈值标准和经济效益,明确东北不同熟期春玉米地膜覆盖种植范围,形成春玉米地膜覆盖综合适宜性区划。东北地区春玉米不同生育阶段地膜覆盖耕作层土壤温度（土壤含水率）与裸地土壤温度（土壤含水率）及产量与≥10 ℃积温存在良好的线性关系（P < 0.01）。基于春玉米地膜覆盖适宜性的评价体系,计算了地膜覆盖适宜指数,完成东北地区春玉米地膜覆盖综合适宜分区。作物地膜覆盖适宜性相关研究可为中国地膜投入量零增长和地膜污染综合防控提供可靠技术支撑。     

消费者生鲜水果线上购买迁徙行为及其影响因素

为探索消费者生鲜水果线上购买迁徙行为的影响因素和机制路径,本研究基于PPM理论,构建结构方程模型分析了消费者生鲜水果线上购买迁徙行为及其影响因素,并运用多群组分析不同消费者行为影响因素的差异性。结果表明：1）价格、信息、服务不满意构成正向影响消费者生鲜水果线上购买迁徙行为的推力因素;感知有用性、搜索、消费体验感构成正向影响迁徙行为的拉力因素;程序转移成本构成负向影响迁徙行为的锚定因素。2）锚定因素负向调节推力因素对迁徙行为的作用效果。3）性别、年龄、学历水平三个人口统计特征在不同假设路径中的影响较为显著。其中女性、30岁及以下、学历水平大专以上的群体更愿意在线上渠道购买生鲜水果。结论丰富了生鲜水果领域消费者迁徙行为的研究成果,并为生鲜电商可持续发展提供了启示。     

柑橘溃疡病拮抗菌的分离筛选及其田间防效

从湖南衡阳、常德、长沙、永州等地的柑橘园采集标本,分离、筛选柑橘叶表微生物,共分离到225株细菌,9株真菌.平板拮抗试验结果表明,其中6个菌株有较好的防效,占分离菌株的13.7%.通过对菌株过滤液和定殖力的测定,菌株Kx15和Kx48效果明显,在温室对柑橘溃疡病的防治效果分别达到45.6%和55.6%,在重病果园中的防治效果分别为40.3%,47.7%,且Kx48要优于Kx15.菌株Kx15初步鉴定属于葡萄糖杆菌属,Kx48属于乳酸杆菌属.     

关于大庆市河蟹养殖情况的调查

文章通过对大庆市杜蒙、林句、肇源三县的实地调查,全面介绍了这些地方河蟹养殖的基本情况、突出特点,分析了河蟹养殖发展过程中存在的制约因素和问题,结合大庆市的实际情况,提出了今后发展河蟹养殖业的相关对策建议。     

An ecosystem-based assessment of the Korean blue crab trammel net fishery in the Yellow Sea and management implications

Landings in the blue crab, Portunus trituberculatus, fishery in Korean waters of the Yellow Sea have declined substantially from 11,000 t in the 1980s to 2,300 t in 2004. Blue crab habitat quality in the Yellow Sea has been degraded by anthropogenic activities including sand mining, land reclamation, and coastal pollution. Various traditional management measures have been implemented, including closed seasons during spawning and size limits, but these measures alone have been unsuccessful to conserve blue crab stocks. Consequently, a total allowable catch and a stock-rebuilding program using an ecosystem-based management approach were implemented in 2003 and 2006, respectively to rebuild blue crab stocks and restore habitats. This program involved assessment of both blue crab stock status and trammel-net fishery impacts at an ecosystem-level using an ecosystem-based fisheries assessment method ( [Zhang et al., 2009] and [Zhang et al., 2010]), which considered fishery data from catch and effort time-series, crab population biology, and ecosystem characteristics, including habitats and environmental conditions. Recent (2008) management status indices have shown significant positive change compared to conditions in 2000 with respect to sustainability of the stock and fishery and with regards to biodiversity and ecosystem habitat quality.     

天津重点水域底栖生物调查分析

于2018年9月,对天津大港,宝坻,华苑等地区的12个站点的大型底栖生物的群落结构及生物多样性进行了调查分析。结果表明,调查区共鉴定出大型底栖生物3门10种,其中软体动物4种,环节动物4种,水生昆虫2种,各站位丰度波动范围为20～840 ind/m^2,平均为205.33 ind/m^2,生物量范围为0.012～308.63 g/m^2,平均为59.69 g/m^2;囊螺为该地区绝对优势种。丰富度指数Margalefs.index在1.48～3.334之间波动, Goodnight修正指数在0.523～1之间波动。     

以法兴渔、促进渔业又好又快发展——就《河北省渔业条例》实施采访河北省农业厅副厅长、河北省水产局局长王进

为加强渔业资源保护、增殖、开发和合理利用,维护渔业生产者的合法权益,保障渔业生产安全和水产品质量安全,促进渔业发展,经修订后的《河北省渔业条例》,于2007年11月23日省十届人大常委会第三十一次会议审议通过,2008年1月1日起施行。