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971.
Ponder J Yoo BH Abraham AD Li Q Ashley AK Amerin CL Zhou Q Reid BG Reigan P Hromas R Nickoloff JA LaBarbera DV 《Marine drugs》2011,9(11):2397-2408
Type IIα DNA topoisomerase (TopoIIα) is among the most important clinical drug targets for the treatment of cancer. Recently, the DNA repair protein Metnase was shown to enhance TopoIIα activity and increase resistance to TopoIIα poisons. Using in vitro DNA decatenation assays we show that neoamphimedine potently inhibits TopoIIα-dependent DNA decatenation in the presence of Metnase. Cell proliferation assays demonstrate that neoamphimedine can inhibit Metnase-enhanced cell growth with an IC(50) of 0.5 μM. Additionally, we find that the apparent K(m) of TopoIIα for ATP increases linearly with higher concentrations of neoamphimedine, indicating ATP-competitive inhibition, which is substantiated by molecular modeling. These findings support the continued development of neoamphimedine as an anticancer agent, particularly in solid tumors that over-express Metnase. 相似文献
972.
Ronald M Bonett Kenneth H Kozak David R Vieites Alison Bare Jessica A Wooten Stanley E Trauth 《BMC ecology》2007,7(1):7
Background
In most regions of the world human influences on the distribution of flora and fauna predate complete biotic surveys. In some cases this challenges our ability to discriminate native from introduced species. This distinction is particularly critical for isolated populations, because relicts of native species may need to be conserved, whereas introduced species may require immediate eradication. Recently an isolated population of seal salamanders, Desmognathus monticola, was discovered on the Ozark Plateau, ~700 km west of its broad continuous distribution in the Appalachian Mountains of eastern North America. Using Nested Clade Analysis (NCA) we test whether the Ozark isolate results from population fragmentation (a natural relict) or long distance dispersal (a human-mediated introduction). 相似文献973.
Zusammenfassung Knollen einer krebsresistenten Sorte wurden mit γ-Strahlen, Nitrosomethylharnstoff und mit beiden Mutagentien kombiniert behandelt.
Im Ergebnis traten Nachkommen mit unterschiedlichem Anf?lligkeitsgrad auf. Bei einem Teil ‘normalisierte’ sich die Resistenz
in der vM3 bzw. vM5. Unter 550 untersuchten Subklonen waren 3 Mutanten (0,55%), die noch nach 6 Prüfjahren (vM8) Defekte im Resistenzmechanismus zeigten. 相似文献
974.
Zusammenfassung Die Nachkommenschaften eines Diallels mit 10 Eltern, ohne reziproke Kreuzungen, wurden gegen Pathotyp 1 (Dl) des Kartoffelkrebses
geprüft. Aufgrund der Aufspaltung in resistent und anf?llig lassen sich 5 genotypische Gruppen unterscheiden. Zwei Gruppen
entsprechen in ihrer Resistenzvererbung dem einfaktoriellen Modell bei Autotetraploiden auf duplexer bzw. simplexer Basis.
Der Nachweis von zwei weiteren Genotypen, die sich nicht einordnen lassen, l?\t eine h?here genetische Varianz erkennen, als
nach dem autotetraploiden Modell theoretisht zu erwarten ist. Die M?glichkeit der Identifizierung weiterer Genotypen wird
nicht ausgeschlossen.
Summary The progeny of a diallel cross between 10 clones of table potatoes were tested for resistance toSynchytrium endobioticum Pathotype 1. Of the 10 parent clones, 9 were resistant, based on the characteristic defence necrosis, and one was very susceptible. In total 45 hybrid and 10 selfed progenies were tested with 60–100 genotypes in each case. The mean performance of each clone was assessed using the percentages of resistant genotypes for all 9 combinations with other clones, and the selfs. The 10 clones are arranged in Table 1 according to their rankings for this parameter. Five groups of genotypes (I–V) can be recognized; these are significantly different from one another. The mean values of the selfs for individual groups, and the combinations with the susceptible parent are also given. From these results it can be concluded that the clones in Groups I–III inherited resistance as if controlled by a single major dominant gene in the simplex or duplex condition in the autotetraploid. The susceptible parent gave a nulliplex-type. The corresponding test results given in Table 2 confirm these conclusions. According to Table 1, clones (4) and (9) do not fit into Groups I and III. They have another genotype. Since the parents used here cover only a small range of possible genotypes, no generally valid statements can be made about the number of groups and the frequency of the genotypes in the groups for all cultivars and clones ofSolanum tuberosum. Inheritance studies of wart resistance by other authors confirm the assumption that further genotype groups may be identified. Greater genetic variation can be expected than is theoretically possible in the single factor model of autotetraploidy.相似文献
975.
Summary The activities of lipolytic acyl-hydrolases (LAH) and lipoxygenases (LOX) were compared in stored tubers of potato cultivars
resistant (Acresta, Eba, Pentland Envoy) and susceptible (Kastor, Pana, Tasso) to post-wounding autolysis. In most cultivars,
LAH activities had reached a maximum by the end of December but in cv. Kastor activity continued to increase throughout the
storage period. LOX activities increased during most of the storage period except in cv. Tasso. The level of the fatty acid
hydroperoxides, assumed from determinations of the malonaldehyde level, also increased during storage.
This work was supported by Project CPBP 05.02.2.10 financed by the Polish Academy of Science. 相似文献
976.
Maria Mayer de Scurrah W. F. Mai R. L. Plaisted 《American Journal of Potato Research》1973,50(2):58-61
Thirteen resistant selections ofS. tuberosum hybrids bred in the U.S.A. and Europe and 44 diploid clones derived fromS. vernei, S. spegazzinii, S. neohawkesii, andS. sanctaerosae each with resistance to one or more pathotypes ofH. rostochiensis were tested for their resistance to three Peruvian populations of this nematode. Only one diploid clone ofS. sanctatrosae was found to be resistant to the three populations of nematodes, but several clones were resistant to one or another of the nematode populations. More clones were found to resist the nematode population collected near Cuzco than those populations collected near Huancayo or Otuzco. Morphometric measurements of body length, stylet length, and distance of median valve to excretory pore, of second stage larvae showed great variation and no clear difference among the three populations. The measurements obtained were closer to pathotype E from England than to any other recognized pathotypes. 相似文献
977.
Brito Giovani G. Concenço Germani Costa Vladimir E. Fagundes Paulo Ricardo R. da Silva-Filho João Luis Parfitt José Maria B. Magalhães Ariano Silva Giovana T. Jardim Thaís M. Luccas Nathália Furtado Scivittaro Walkyria B. 《Journal of Crop Science and Biotechnology》2022,25(1):19-32
Journal of Crop Science and Biotechnology - Phenotyping is the major bottleneck in the effort to develop varieties of rice (Oryza sativa L.) suitable for growing under a water-saving irrigation... 相似文献
978.
Flávia E. de Mello Valéria S. Lopes-Caitar Sheila A. Xavier-Valencio Helen P. da Silva Sören Franzenburg Andreas Mehl Joseph-Alexander Verreet Maria I. Balbi-Peña Francismar C. Marcelino-Guimaraes Cláudia V. Godoy 《Plant pathology》2022,71(2):373-385
Fungicide sprays on soybean in Brazil have contributed to the selection of less sensitive isolates of Corynespora cassiicola. We collected 59 isolates of C. cassiicola from three Brazilian states and two isolates from Paraguay. We investigated their EC50 to quinone outside inhibitors (QoI) and methyl benzimidazole carbamate (MBC), any cross-resistance to compounds within QoI and MBC groups, and characterized the polymorphisms in their cytb and β-tubulin genes. Local associations of polymorphisms identified in each gene were statistically correlated with assays results. In total, 79% and 74% of the isolates were classified as resistant to QoI and MBC fungicides, respectively. There was positive cross-resistance to active ingredients within QoI and MBC groups. For QoI, all isolates presented heteroplasmy in G143A of cytb gene; the mutations F129L and G137R were not found. For MBC, 63% of isolates possessed E198A and 21% possessed F200Y mutations, associated with reduced control by MBC fungicides. Heteroplasmy was identified in two and one isolates from Brazil with E198A and F200Y mutations, respectively. The resistance factor for isolates with E198A (10.9) was statistically similar to the isolate with F200Y (8.8) mutation. Genic association analysis of the in vitro assays using discriminatory doses proved them to be accurate. Reduced sensitivity of C. cassiicola to QoI and MBC was also identified in isolates from Paraguay and resistance to QoI and MBC was widely present in C. cassiicola isolates from the main soybean-producing states in Brazil. Thus, integrated management measures should be adopted to manage soybean target spot in these countries. 相似文献
979.
Barbosa Douglas Rafael e Silva dos Santos Rebeca Barbosa Vieira Santos Flavia Mayara Pereira da Silva Junior Pedro Justino de Oliveira Neto Francisco Marques Silva Gutierres Nelson de Andrade Dutra Kamilla do Amaral Ferraz Navarro Daniela Maria 《植物病害和植物保护杂志》2022,129(1):125-136
Journal of Plant Diseases and Protection - This study aimed to investigate the effects of the essential oils of Cymbopogon flexuosus and Alpinia zerumbet in the treatment of cowpea [Vigna... 相似文献
980.
Journal of Plant Diseases and Protection - In the present study we examined the effect of the presence of wheat bugs (Eurygaster spp. and Aelia spp.) on wheat on the mortality, progeny production... 相似文献