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171.
Shiori Okuda Mitsuru Okuda Shohei Matsuura Shinichiro Okazaki Hisashi Iwai 《European journal of plant pathology / European Foundation for Plant Pathology》2013,136(2):355-362
The vector competence of Frankliniella occidentalis for Chrysanthemum stem necrosis virus (CSNV) was evaluated. Three vector strains with distinct competences for Tomato spotted wilt virus (TSWV) transmission were investigated, including an artificially selected strain (TsH) that has a particularly high competence (>90 %). Newly hatched larvae of F. occidentalis were given an acquisition access period of 5 days on CSNV-infected D. stramonium leaves, and reared to maturity. Their transmission efficiencies were examined using a leaf disk assay using Petunia x hybrida leaves. Following the leaf disk assay, the virus accumulation in the vectors was examined via a double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) of their bodies. The results showed that the CSNV acquisition and transmission efficiency of the TsH strain did not differ from those of the others, indicating that the competence of F. occidentalis as a vector for CSNV is not related to that for TSWV. The CSNV transmission and acquisition efficiencies of two F. intonsa strains (Hiroshima and Fukuoka) were also evaluated. In Hiroshima strain, 35 % of adults were viruliferous, but only two transmitters (3 %) were observed. In Fukuoka strain, 6 % were viruliferous, and no transmitters were observed. These results indicate that F. intonsa cannot be a major vector for CSNV. The accumulation of CSNV in the adults of F. occidentalis and F. intonsa evaluated using DAS-ELISA showed a significant difference in ELISA values among transmitter, viruliferous non-transmitter, and non-viruliferous individuals. These results clearly demonstrated that only transmitters that accumulated a threshold quantity of virus can transmit CSNV to plants. 相似文献
172.
The question of what determines plant community composition is fundamental to the study of plant community ecology. We examined
the relative roles of historical land use, landscape context, and the biophysical environment as determinants of plant community
composition in regenerating citrus groves in north-central Florida. Results were interpreted in light of plant functional
traits. Herbaceous and woody plants responded differently to broad-scale variables; herbs correlated most strongly with surrounding
land cover at a scale of 8 km, while the only significant determinant of woody species distributions was local land use history.
There were significant correlations between herbaceous species and spatial context, habitat isolation, environmental variables,
and historical variables. Partial Mantel tests indicated that each variable provided a unique contribution in explaining some
of the variation in the herbaceous dataset. The correlation between woody plants and local historical variables remained significant
even with other effects corrected for. In the herbaceous community, species composition was linked to functional traits much
as expected from classical theory. While spatial influences in our study system are important for both woody and herbaceous
plants, the primary determinant of plant community composition in regenerating citrus groves is historical land use. Our results
suggest that the fine-scale mechanisms of local competition, tolerance and facilitation invoked by many classical studies
may ultimately be less important than land use history in understanding current plant community composition in regenerating
agricultural areas. 相似文献
173.
The discipline of landscape ecology recognizes the importance of measuring habitat suitability variables at spatial scales
relevant to specific organisms. This paper uses a novel multi-scale hierarchical patch delineation method, PatchMorph, to
measure landscape patch characteristics at two distinct spatial scales and statistically relate them to the presence of state-listed
endangered yellow-billed cuckoos (Coccyzus americanus occidentalis) nesting in forest patches along the Sacramento River, California, USA. The landscape patch characteristics calculated were:
patch thickness, area of cottonwood forest, area of riparian scrub, area of other mixed riparian forest, and total patch area.
A third, regional spatial variable, delineating the north and south portions of study area was also analyzed for the effect
of regional processes. Using field surveys, the landscape characteristics were related to patch occupancy by yellow-billed
cuckoos. The area of cottonwood forest measured at the finest spatial scale of patches was found to be the most important
factor determining yellow-billed cuckoo presence in the forest patches, while no patch characteristics at the larger scale
of habitat patches were important. The regional spatial variable was important in two of the three analysis techniques. Model
validation using an independent data set of surveys (conducted 1987–1990) found 76–82% model accuracy for all the statistical
techniques used. Our results show that the spatial scale at which habitat characteristics are measured influences the suitability
of forest patches. This multi-scale patch and model selection approach to habitat suitability analysis can readily be generalized
for use with other organisms and systems. 相似文献
174.
The viability of metapopulations in fragmented landscapes has become a central theme in conservation biology. Landscape fragmentation
is increasingly recognized as a dynamical process: in many situations, the quality of local habitats must be expected to undergo
continual changes. Here we assess the implications of such recurrent local disturbances for the equilibrium density of metapopulations.
Using a spatially explicit lattice model in which the considered metapopulation as well as the underlying landscape pattern
change dynamically, we show that equilibrium metapopulation density is maximized at intermediate frequencies of local landscape
disturbance. On both sides around this maximum, the metapopulation may go extinct. We show how the position and shape of the
intermediate viability maximum is responding to changes in the landscape’s overall habitat quality and the population’s propensity
for local extinction. We interpret our findings in terms of a dual effect of intensified landscape disturbances, which on
the one hand exterminate local populations and on the other hand enhance a metapopulation’s capacity for spreading between
habitat clusters. 相似文献
175.
Previous comparative studies on patterns of reproduction in small-mammal species focus primarily on latitudinal differences
in average litter size. Few studies compare reproductive patterns among northern and southern populations at the landscape
scale. Our study compares differences in seasonal patterns of reproduction in northern and southern populations of the golden
mouse, Ochrotomys nuttalli, and the white-footed mouse, Peromyscus leucopus. These are remarkably similar species with regard to bioenergetics, body mass, feeding behavior, home-range size, natural
history, nest-site preference, and periods of activity. Both species also exhibit very similar intraspecific seasonal patterns
of reproduction across their respective geographic ranges. We found that O. nuttalli and P. leucopus switch from a summer breeding season, extending from late spring through early autumn in the north to a winter breeding season
extending from late autumn through early spring in the south, near the isotherm where mean annual temperature is 15.6°C (60°F),
or approximately 35° N latitude. This latitudinal isotherm provides a geographic benchmark to address future changes in patterns
of reproduction attributed to climate change. Findings also suggest that length of the breeding season and patterns of reproduction
between species partially explain why P. leucopus is typically more abundant than O. nuttalli in similar habitat types. 相似文献
176.
Brian R. Miranda Brian R. Sturtevant Jian Yang Eric J. Gustafson 《Landscape Ecology》2009,24(5):587-598
We demonstrate a method to evaluate the degree to which a meta-model approximates spatial disturbance processes represented
by a more detailed model across a range of landscape conditions, using neutral landscapes and equivalence testing. We illustrate
this approach by comparing burn patterns produced by a relatively simple fire spread algorithm with those generated by a more
detailed fire behavior model from which the simpler algorithm was derived. Equivalence testing allows objective comparisons
of the output of simple and complex models, to determine if the results are significantly similar. Neutral landscape models
represent a range of landscape conditions that the model may encounter, allowing evaluation of the sensitivity and behavior
of the model to different landscape compositions and configurations. We first tested the model for universal applicability,
then narrowed the testing to assess the practical domain of applicability. As a whole, the calibrated simple model passed
the test for significant equivalence using the 25% threshold. When applied to a range of landscape conditions different from
the calibration scenarios, the model failed the tests for equivalence. Although our particular model failed the tests, the
neutral landscape models were helpful in determining an appropriate domain of applicability and in assessing the model sensitivity
to landscape changes. Equivalence testing provides an effective method for model comparison, and coupled with neutral landscapes,
our approach provides an objective way to assess the domain of applicability of a spatial model. 相似文献
177.
Tsutomu MATSUMOTO Yuichiro NARA Hiromitsu FURUYA Harumi TAKAHASHI Kiichi TAIRAKO Hideki YAMAMOTO 《Journal of General Plant Pathology》2002,68(4):382-384
L11A-Fukushima (L11A-F) derived from attenuated isolate LuA of Tomato mosaic virus (ToMV) has the highest ability to cross protect against virulent ToMV among LuA and its derivatives and is stably inherited.
Growth, yield, fruit quality and symptom attenuation of inoculated tomato plants did not differ significantly between L11A-F and L11A. The infectivity of progeny viruses in tomato infected with LuA-F was less than 4% of that with virulent ToMV. From these
results, L11A-F appears to possess the properties necessary for practical use. To manage L11A-F strictly, a PCR-based assay to detect trace contamination of virulent ToMV in L11A-F preparations was established.
Received 10 June 2002/ Accepted in revised form 30 October 2002 相似文献
178.
M. Lodovica Gullino Riccardo Savigliano Giuliana Gasparrini Corrado Clini 《Phytoparasitica》2007,35(4):321-329
Methyl bromide (MB) was phased out on January 1, 2005, and a Critical Use Exemption (CUE) process has been established in
order to define the rules for allocating the needed amounts of the fumigant. This paper deals with the effects of the CUE
process on Italian horticulture. The actual usage of the amounts of MB allocated to Italy for the year 2005 in the different
areas and on the economically most important crops was monitored and critically evaluated. The usage pattern monitored shows
that already in the first year after MB phaseout, Italy was able to replace it completely on crops such as zucchini, salad
greens, basil and watermelon. However, on crops such as tomato, pepper, melon, eggplant, strawberry, and cut flowers, further
work is required in order fully to replace MB. The emerging problems and research needs are discussed briefly. 相似文献
179.
Judith Hübschen Lilo Kling Ulrike Ipach Volker Zinkernagel Derek Brown Roy Neilson 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(9):883-891
The nematode species Longidorus attenuatus, L. elongatus, L. macrosoma and Paralongidorus maximusare economically important pests to the viticulture industry due to their ability to vector two nepoviruses (Raspberry Ringspot Virus and Tomato Black Ring Virus) to grapevines. In Germany, these species occur in vineyard soil with other non-vector but morphologically similar longidorid species, L. helveticus, L.profundorum and L. sturhani. Species-specific primers were designed from ribosomal DNA for all seven species to facilitate taxonomic identification for non-specialists. Primers were assessed for their reliability by screening, where possible, a number of populations of each species. Furthermore, their selectivity and sensitivity were determined when challenged with closely related longidorid species and general nematode communities typical of vineyard soil. A multiplex approach using a common forward primer combined with species-specific reverse primers enabled three target nematode species to be detected in the same PCR reaction. All primers were highly specific, detecting all nematode developmental forms from disparate populations and were sufficiently sensitive to detect a single target nematode within a whole nematode community typical of a vineyard soil comprising of a range of non-target species. Given their specificity, sensitivity and reliability, these diagnostic primers should be of great benefit to both phytosanitary/quarantine services related to the viticulture industry and also as a decision management tool for growers. 相似文献
180.
Toghyani M Toghyani M Shahryar HA Zamanizad M 《Tropical animal health and production》2011,43(6):1183-1189
The objective of this study was to evaluate the effects of two methods of early-age water restriction on performance, some
immune responses, serum metabolites, and prevalence of leg weakness in broiler chicks. One-hundred-eighty-seven-day-old chicks
(Ross 308) were allocated to three treatments with four replicates based on a completely randomized design. Treatments included
the control group, where birds had free access to water, and water-restricted groups, where access to water was limited to
three 2-h periods (WRT1) or a 12-h period (WRT2) per day from days 7 to 17. Chicks in the control group were significantly
heavier (P < 0.05) at 17 and 46 days of age compared to the restricted birds. In the WRT2 group, feed intake decreased significantly
over the 17- to 28-day period while feed conversion ratio was poorer during water restriction (P < 0.05). Antibody titers against Newcastle and SRBC were higher (P < 0.05) for chicks with ad libitum access to water. Birds in the restricted groups exhibited a higher heterophil to lymphocyte
ratio compared to the control group (P < 0.05). The treatments had no significant impact on serum metabolites including protein, triglyceride, total cholesterol,
high-density lipoprotein and low-density lipoprotein cholesterols, calcium, and phosphorus concentrations. Walking ability,
tibial dyschondroplasia, foot pad, hock burn, and valgus/varus angulation were not significantly influenced by water restriction.
In conclusion, the current results indicate that early-age water restriction negatively influenced productive traits and immunological
responses of broiler chicks and failed to have any favorable impacts on leg health. 相似文献