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41.
The effect of beta-cyclodextrin (beta-CD) on the removal of the herbicide norflurazon (NFL) from soils has been investigated. The interaction of NFL with beta-CD in solution yielded the formation of a water-soluble inclusion complex at 1:1 stoichiometric ratio, which gave an increase in NFL solubility. Desorption studies of NFL previously adsorbed on six soils of different characteristics have been performed in the presence of 0.01 M beta-CD or 0.01 M Ca(NO(3))(2) as extractant solutions. Positive hysteresis was observed in all soils when 0.01 M Ca(NO(3))(2) solution was used, indicating that desorption of NFL from these soils was not completely reversible. On the contrary, the application of beta-CD solutions to soils where NFL had been previously adsorbed increased very much its desorption, and a negative hysteresis was obtained for all soils studied; that is, more NFL was desorbed with respect to NFL adsorption isotherm. A clear relationship was observed between the physicochemical characteristics of the soils and the beta-CD concentration necessary to remove the herbicide, the percentages of desorption observed for each soil being inversely related to the values obtained for the Freundlich sorption capacity parameter of the herbicide, K(f). In general, high desorption yields can be obtained with very low beta-CD concentrations, which is an important advantage from an economic point of view, although in those soils that present an extremely high NFL adsorption, higher amounts of beta-CD should be used. The results obtained indicate the high extracting power of beta-CD toward the herbicide previously adsorbed on the soils and the potential use of beta-CD for in situ remediation of pesticide-contaminated soils. 相似文献
42.
Participatory landrace selection for on-farm conservation: An example from the Central Valleys of Oaxaca, Mexico 总被引:8,自引:1,他引:8
Mauricio R. Bellon Julien Berthaud Melinda Smale José Alfonso Aguirre Suketoshi Taba Flavio Aragón Jaime Díaz Humberto Castro 《Genetic Resources and Crop Evolution》2003,50(4):401-416
On-farm conservation is recognized as a key component of a comprehensive strategy to conserve crop genetic resources. A fundamental problem faced by any on-farm conservation project is the identification of crop populations on which efforts should be focused. This paper describes a method to identify a subset of landraces for further conservation efforts from a larger collection representing the diversity found in the Central Valleys of Oaxaca, Mexico. Mexico is a center of origin and diversity for maize (Zea mays L.). The 17 landraces selected from an initial collection of 152 satisfy two criteria. First, they represent the diversity present in the larger collection. Second, they appear to serve the interests of farmers in the region. Data for applying the method were elicited through participatory as well as conventional techniques. They incorporate the complementary perspectives of both men and women members of farm households, and of plant breeders and social scientists. 相似文献
43.
Aerosol samples were continuously collected at Rishiri Island, Japan and concentrations of trace metals and water-soluble ionic constituents in the aerosol samples were measured in this study. Trace metal concentrations were analyzed by using inductively coupled plasma mass spectrometry equipped with a laser ablation sample introduction (LA/ICP-MS), which is a rapid and simultaneous method for multi-element analysis. The average concentrations of trace metals and water-soluble ionic constituents in TSP at Rishiri Island from March 2001 to October 2003 were: Al: 251 ± 371 (n= 359), Ti: 27 ± 38 (n= 342), V: 1.3 ± 1.6 (n= 327), Cr: 2.0 ± 2.2 (n= 202), Mn: 8.6 ± 12.0 (n= 353), Fe: 356 ± 645 (n= 349), Co: 0.18 ± 0.25 (n= 250), Ni: 1.9 ± 3.4 (n= 232), Cu: 7.4 ± 10.2 (n= 123), Zn: 16 ± 20 (n= 242), As: 1.2 ± 2.1 (n= 312), Se: 0.49 ± 0.65 (n= 133), Cd: 0.19 ± 0.33 (n= 285), Sb: 0.46 ± 0.62 (n= 265), Pb: 16 ± 30 (n= 314), Na+: 1153 ± 1384 (n= 306), NH4 +: 247 ± 257 (n= 254), K+: 74 ± 63 (n= 284), Ca2+: 144 ± 174 (n= 294), Mg2+: 150 ± 161 (n= 293), Cl?: 2703 ± 3151 (n= 180), NO3 ?: 339 ± 415 (n= 332), SO4 2?: 1648 ± 1398 (n= 362) (unit: ng m?3). The obtained trace metal concentration levels in the aerosol were 1/10–1/70 of those obtained at Beijing, China and 1/2–1/40 of those obtained at Tokyo, Japan. The correlation between Al, Ti, Mn, and Fe, which were derived mainly from soil dust, was high at Rishiri Island. High-concentration events (when the measured value exceeds the average +3σ for the entire observation period) for each chemical constituent were observed several times per year, although the chemical concentration levels in TSP at Rishiri Island were almost constant throughout the year. The high-concentration phenomenon, which occurred in only 2% of the measurement days, raised the average concentration of the atmospheric chemical constituents at Rishiri Island by about 10%. The average value of the Pb/Zn ratio at Rishiri Island during the entire period was 1.8 ± 2.9, which is higher than 1. This suggests that the atmosphere at Rishiri Island has constantly been receiving contaminants from the Asian Continent. We conclude that the chemical constituents in the aerosol at Rishiri Island have been regularly transported from a wide area across the Asian Continent. 相似文献
44.
Villaverde J Morillo E Pérez-Martínez JI Ginés JM Maqueda C 《Journal of agricultural and food chemistry》2004,52(4):864-869
The formulation of inclusion complexes of the herbicide norflurazon as guest and beta-cyclodextrin (beta-CD) as host has been studied as a first step in the use of cyclodextrins to obtain improved formulations of this herbicide. The interaction of norflurazon with beta-CD produced the formation of an inclusion complex in solution and in solid state. The inclusion of norflurazon in beta-CD in solution was studied by phase solubility, and an apparent stability constant of 360 M(-)(1), a 1:1 stoichiometric ratio for the complex, and up to 5-fold increase in norflurazon solubility were determined. Three processing methods (kneading, spray drying and vacuum evaporation) were used to prepare norflurazon-beta-CD solid inclusion complexes. X-ray diffraction, infrared spectroscopy, differential scanning calorimetry, and scanning electron microscopy techniques were used to study the solid complexes. From the different solid systems, an increase of norflurazon aqueous dissolution rate was obtained in comparison to the uncomplexed herbicide. This finding is a first step to obtain controlled release and/or protective formulations of norflurazon, which allow a more rational application of norflurazon, diminishing the use of organic solvents and increasing its efficacy. 相似文献
45.
46.
Pigatto JA Andrade MC Laus JL Santos JM Brooks DE Guedes PM Barros PS 《Veterinary ophthalmology》2004,7(3):205-208
The objective of this study was to examine the endothelial surface morphology and to perform morphometric analysis of the corneal endothelial cells of Yacare caiman (Caiman yacare) using scanning electron microscopy. Morphometric analysis with regard to polygonality, mean cell area, cell density and coefficient of variation of mean cell area was performed. Cell areas were measured using image analysis software. The normal corneal endothelium of Yacare caiman consisted of polygonal cells of uniform size and shape with interdigitations of the cell borders. Microvilli appeared as protrusions on the cellular surface. The average cell area was 270 +/- 24 microm(2) and the endothelial cell density was 3704 +/- 324 cells/mm(2). The coefficient of variation of cell area was 0.22. This study demonstrates that the Yacare caiman corneal endothelium is similar to those described in other vertebrates. 相似文献
47.
A case of a dwarf hamster with two progressively growing nodules on the right fore limb is described. These were excised following ineffective medical treatment and were submitted for histopathological examination, which revealed bacterial pseudomycetoma in both nodules. To the authors' knowledge this is the first reported case of bacterial pseudomycetoma in a dwarf hamster. 相似文献
48.
Mastrorilli C Spangler EA Christopherson PW Aubry OA Newton JC Smith AN Kennis RA Weismann JL Moore PF 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2012,41(3):412-418
A 9‐month‐old male Great Dane had progressive generalized nodular dermatopathy for several months. There were > 100 raised, alopecic, firm, painful nodules throughout the skin. Aspirates from several lesions yielded moderate numbers of irregularly round or polygonal to spindle‐shaped cells with mild to moderate anisocytosis and few inflammatory cells, and the cytologic interpretation was proliferation of mesenchymal or histiocytic cells. On histopathologic examination, nodules were composed of densely packed sheets of round to spindle‐shaped cells with mild anisokaryosis and low mitotic activity. Multifocal histiocytic sarcoma with a spindle‐cell pattern was diagnosed based on morphologic features and intense expression of CD18. Additional immunophenotypic analysis on frozen sections of tissue confirmed the diagnosis of histiocytic sarcoma; expression of CD18, CD45, CD1a, CD11b, and CD11c, limited expression of Thy‐1 (CD90) and CD80, and lack of expression of CD4, CD11d, and CD86 indicated that the cells were likely interstitial dendritic cells; a review of reactive and neoplastic dendritic cells is provided. Based on staging, internal organs were not affected. Sequential treatment with lomustine and doxorubicin failed to prevent progression of the cutaneous lesions, and the dog died 3 months after initial diagnosis. At necropsy, a focus of neoplastic cells was present in one lymph node, but except for skin other organs were not involved. The clinical presentation of histiocytic sarcoma may be unusual, and neoplastic cells may lack overt features of malignancy on cytologic and histopathologic examination. In some instances, immunophenotyping is required to differentiate histiocytic sarcoma from other histiocytic disorders. 相似文献
49.
Oliet Juan A. Artero Francisco Cuadros Simón Puértolas Jaime Luna Lourdes Grau Jose M. 《New Forests》2012,43(5-6):925-939
New Forests - Increasing plant size has been considered a way to improve survival and growth of planted containerized stock under dry conditions. Additionally, deep planting provides advantages to... 相似文献
50.
Jaime Viscasillas Christopher J Seymour David C Brodbelt 《Veterinary anaesthesia and analgesia》2013,40(2):212-219
Objective To compare the success by inexperienced anaesthetists of using a modified infraorbital approach to the maxillary nerve with the traditional percutaneous approach. Study design Prospective, randomized, blinded controlled study. Animals Heads from 37 euthanized Beagle and Beagle cross dogs. Methods Four anaesthetists were recruited to perform two different approaches to block the maxillary nerve of the cadavers. The infraorbital (I) approach advanced an intravenous catheter along the infraorbital canal. Earlier measurements from scans of similar heads were used to assess suitable catheter size. The percutaneous (P) approach introduced a needle percutaneously just below the ventral border of the zygomatic arch. The side of the head where the technique was to be performed was randomized. A total volume of 0.5 mL methylene blue was injected in each approach. After completion of injections, head dissections were performed by an investigator unaware of the approach used and staining of the maxillary and pterygopalatine nerves was evaluated. Chi squared analysis examined the relationship between the methods (p < 0.05). Complications related to the techniques, such as intravascular/intraneural injection and location of the dye, were evaluated macroscopically. Results Maxillary nerve staining >6 mm was found in 64.9% (I) versus 21.6% (P) attempts; staining <6 mm was found in 27% (I) versus 21.6% (P); and no nerve staining 8.1% (I) versus 56.8% (M). Pterygopalatine nerve staining was found in 70% (I) versus 21% (P). The infraorbital approach demonstrated significantly higher maxillary and pterygopalatine nerve staining compared to the percutaneous approach (p = 0.001 for both nerves). No evidence of intravascular/intraneural injections was found. Conclusion and clinical relevance The infraorbital approach was more successful than the percutaneous approach when performed by inexperienced anaesthetists. No macroscopic complications were observed. 相似文献