全文获取类型
收费全文 | 4531篇 |
免费 | 434篇 |
国内免费 | 1篇 |
专业分类
林业 | 403篇 |
农学 | 183篇 |
基础科学 | 32篇 |
1138篇 | |
综合类 | 158篇 |
农作物 | 282篇 |
水产渔业 | 609篇 |
畜牧兽医 | 1675篇 |
园艺 | 97篇 |
植物保护 | 389篇 |
出版年
2024年 | 8篇 |
2023年 | 49篇 |
2022年 | 74篇 |
2021年 | 151篇 |
2020年 | 162篇 |
2019年 | 186篇 |
2018年 | 234篇 |
2017年 | 261篇 |
2016年 | 244篇 |
2015年 | 183篇 |
2014年 | 226篇 |
2013年 | 297篇 |
2012年 | 341篇 |
2011年 | 383篇 |
2010年 | 212篇 |
2009年 | 174篇 |
2008年 | 287篇 |
2007年 | 242篇 |
2006年 | 210篇 |
2005年 | 180篇 |
2004年 | 146篇 |
2003年 | 142篇 |
2002年 | 137篇 |
2001年 | 103篇 |
2000年 | 84篇 |
1999年 | 46篇 |
1998年 | 18篇 |
1997年 | 23篇 |
1996年 | 8篇 |
1995年 | 21篇 |
1994年 | 13篇 |
1993年 | 13篇 |
1992年 | 10篇 |
1991年 | 10篇 |
1990年 | 14篇 |
1989年 | 16篇 |
1988年 | 4篇 |
1987年 | 4篇 |
1986年 | 3篇 |
1985年 | 9篇 |
1984年 | 8篇 |
1983年 | 4篇 |
1982年 | 3篇 |
1980年 | 3篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1970年 | 2篇 |
1934年 | 2篇 |
1926年 | 1篇 |
1925年 | 1篇 |
排序方式: 共有4966条查询结果,搜索用时 15 毫秒
101.
102.
Oscar Goñi María T. Sanchez-Ballesta Carmen Merodio María I. Escribano 《Postharvest Biology and Technology》2010,55(3):169-173
In order to obtain a better understanding of the active defense strategy of cherimoya (Annona cherimola Mill.) fruit, hydrolytic and antifungal activity, as well as expression of proteins functionally and immunogenically related to the pathogenesis-related proteins chitinase (PR-Q) and 1,3-β-glucanase (PR-2), were estimated in fruit at different ripening stages. Increase in expression of the 27 kDa constitutive chitinase and the induction of two new proteins, a 26 kDa chitinase and a 51 kDa 1,3-β-glucanase were associated with enhanced in vitro hydrolytic and antifungal activity of the acidic protein extract in ripe fruit. Ripening modified the expression of constitutive basic isoenzymes, with a sharp decrease in both relative accumulation and hydrolytic activity. Likewise, a new basic 33 kDa chitinase was induced in the over-ripe fruit, concomitant with accumulation of a basic constitutive 76 kDa 1,3-β-glucanase. At this stage, the basic protein extract modified in vitro growth inhibition of Botrytis cinerea. Short-term high CO2 treatment delayed fruit ripening and maintained a similar distribution of activity and isoenzymatic pattern in both protein fractions to that in unripe fruit. These results indicate that the changes in the pattern of defense proteins and hydrolytic activity in cherimoyas appear to be associated with ripening. Moreover, unlike the constitutively expressed isoenzymes, only the transitorily induced chitinases and 1,3-β-glucanases were associated with an active defense-related response. 相似文献
103.
The present work evaluated the chemical composition of the essential oils (EO) obtained from Lippia origanoides and their DNA protective effect against bleomycin-induced genotoxicity. L. origanoides EO chemical composition was determined by gas chromatography–mass spectrometry (GC–MS). The major compounds of the L. origanoides EOs were thymol (34–58%) and carvacrol (26%). The antigenotoxic effects of the EOs, major compounds and standard compound (epigallocatechin gallate) were assayed in co-incubation procedures using the SOS chromotest in Escherichia coli. Both EOs and their major compounds protected bacterial cells against bleomycin-induced genotoxicity indicating that these two compounds were principally responsible for the antigenotoxicity detected in the oils. Thymol and carvacrol antigenotoxicity was lower than those observed with epigallocatechin gallate. The results were discussed in relation to the chemopreventive potential of L. origanoides EOs and their major components, carvacrol and thymol. 相似文献
104.
Oscar González‐Davis Elizabeth Ponce‐Rivas M. Del Pilar Sánchez‐Saavedra María‐Enriqueta Muñoz‐Márquez William H. Gerwick 《Journal of the World Aquaculture Society》2012,43(3):387-399
Vibrio spp. are the most common and harmful shrimp pathogenic bacteria; however, microalgae and cyanobacteria have the ability to produce antimicrobial substances against these species. In this study, the organic and aqueous extracts of 28 species of marine microalgae and cyanobacteria were screened against Vibrio campbellii M1. Two of these phytoplankton species with antibacterial activity in aqueous extracts (Dunaliella tertiolecta and Skeletonema costatum) and nontoxic to brine shrimp Artemia franciscana nauplii were used to evaluate their anti‐Vibrio effect when used as green‐water cultures in Vibrio‐challenged white shrimp Litopenaeus vannamei cultures. No differences in mortality of juvenile L. vannamei were observed between treatments tested, suggesting that the pathogenicity of V. campbellii could be related to the growth stage of shrimp. The proximal composition of D. tertiolecta and S. costatum was in the recommended range for penaeid shrimp nutrition, allowing shrimp supplemented with these microalgae to have significantly greater total length and weight than control shrimp. Shrimp supplemented with S. costatum presented the highest values of organic mass (11.48 mg/organism) and growth rate (0.31 mg/d) in comparison to D. tertiolecta. These results indicate that microalgae are not only capable of producing antibacterial compounds against Vibrio but can also help shrimp nutrition. 相似文献
105.
Romualdo Muoz Antonio Martínez-Martínez Alfonso Ros-Barcel M. Angeles Pedreo 《Pest management science》1990,30(2):235-241
4-Amino-6-methyl-3-phenylamino-1,2,4-triazin-5(4H)-one alters the integrity of chloroplasts in barley (Hordeum vulgare L. var. Hassan) leaves as judged by their sedimentation profiles in linear gradients of sucrose. This effect may be related to the inhibition caused by 4-amino-6-methyl-3-phenylamino-1,2,4-triazine-5(4H)-one of both ascorbate peroxidase and superoxide dismutase activities, and could thus be explained by an accumulation of toxic reduced-oxygen species in illuminated chloroplasts. 相似文献
106.
Olby NJ De Risio L Muñana KR Wosar MA Skeen TM Sharp NJ Keene BW 《American journal of veterinary research》2001,62(10):1624-1628
OBJECTIVES: To develop and compare the reliability of 2 methods of scoring pelvic limb gait in dogs recovering from thoracolumbar spinal cord injuries and to use this scoring system to determine the rate and level of functional recovery of dogs with acute thoracolumbar intervertebral disk herniations. ANIMALS: 46 dogs with spinal cord injuries resulting from intervertebral disk herniations. PROCEDURE: Dogs' gaits were videotaped at different time intervals after injury. In phase 1 of the study, the stages of recovery of pelvic limb function were identified, and a numeric scoring system was devised to reflect that recovery. In phase 2, pelvic limb gait was scored by different observers, using a numeric and a visual analog scale. Intra- and interobserver coefficients of variability of both methods were compared. In phase 3, pelvic limb function was scored, using the numeric scale at various intervals after acute thoracolumbar disk herniations. RESULTS: The numeric scale was significantly more reliable than the visual analog scale when both intra- and interobserver coefficients of variability were evaluated. Dogs that were paraplegic with no deep pain sensation recovered at different rates during the first 3 months, whereas dogs that were paraplegic with deep pain sensation typically recovered within 1 month of injury. CONCLUSIONS: Pelvic limb gait of dogs recovering from thoracolumbar spinal cord injuries can be reliably quantified, using a numeric scale. This scale will facilitate the performance of clinical trials aimed at improving the outcome of acute spinal cord injuries. 相似文献
107.
Ferreira-Dias GM Serrão PM Durão JF Silva JR 《American journal of veterinary research》2001,62(4):526-530
OBJECTIVE: To document uterine growth and microvascular development in the endometrium of uteri with differing degrees of fibrosis as well as uterine growth throughout the estrous cycle of mares. ANIMALS: 30 mares. PROCEDURE: Uterine tissue was obtained during the breeding season from a slaughter facility. Stage of estrous cycle of the mares was assessed on the basis of ovarian structures and plasma progesterone concentrations. Endometrium was characterized by use of light microscopy, and blood vessel walls were marked by histochemical techniques. Microvascular development was evaluated by a computerized image analysis system. Growth of uterine tissue was based on cellular content of DNA and RNA, RNA:DNA, and protein:DNA. RESULTS: Significant differences in vascular density were not observed in the endometrium of uteri obtained from mares euthanatized during the follicular or luteal phase of the estrous cycle, regardless of whether endometrial classification of degree of fibrosis was considered. There was a 3-fold increase in amount of DNA and RNA of endometrial cells in the follicular phase when compared to myometrium. Hypertrophy of endometrial tissue during the luteal phase was reflected by a significant increase in cell protein content and protein:DNA. CONCLUSIONS AND CLINICAL RELEVANCE: Endometrial growth of vascular tissues during the estrous cycle may be coordinated with development of nonvascular tissue. Estrogen and progesterone may play a role in regulation of uterine growth and angiogenesis. 相似文献
108.
Crude protein (CP) of forages can be separated into fractions of differentiated abilities to provide available amino acids in the lower gut of ruminants. This knowledge is critical to develop feeding systems and to predict animal responses. We have measured during two growing seasons (1996 to 1997 and 1997 to 1998) the CP fractions of common vetch fresh forage with the objective being to assess the influence of maturity on concentration of CP fractions (as a percentage of total CP) and fraction yields. Fraction B2, which represents true protein of intermediate ruminal degradation rate, was the largest single fraction in common vetch forage (about 40% of CP across seasons and maturity stages). Soluble fractions (A plus B1) were less than 50% of total CP while the unavailable fraction C ranged from 4 to 8% of total CP. As a result, the remaining fraction B3 (true protein of very low degradation rate) only represented 2 to 9% of total CP. Concentration and yield of fraction B3 increased (P < 0.05) from flowering to pod-filling. Results showed that undegraded dietary protein represented a small proportion of total CP in common vetch forage. Moving the harvesting stage from flowering to the pod filling phase allowed for greater yield of undegraded dietary protein. 相似文献
109.
Ortiz J Ruiz de Ybañez MR Abaigar T Goyena M Espeso G Cano M Alonso F 《The Veterinary record》2001,149(1):12-15
Seven different methods of administering ivermectin to gazelles were compared: subcutaneous injection, direct oral administration, administration in individual feeds, administration in a herd feed, direct oral administration of a second ivermectin formulation, administration in individual water supplies, and administration in the herd's water supply. The first five treatments were effective, as monitored by faecal egg count reduction tests, and administration in individual feeds or in a herd feed avoided the need to capture the animals, with the attendant risk of mortality. Of the factors associated with the recipients (species, sex, age and inbreeding coefficient) age was the only significant factor for the efficacy of the treatment. Oral or subcutaneous, individual or collective, and direct or indirect administrations were equally satisfactory for the treatment of all the parasite groups studied. Only when parasitic problems were due to Nematodirus species did direct administration to individual animals appear to be preferable. 相似文献
110.
An outbreak of Fusobacterium necrophorum-induced septicemia occurred in a group of 40 captive wild-caught pronghorns (Antilocapra americana). Primary pododermatitis or necrotic stomatitis progressed to produce fatal septicemia with metastatic lesions in the forestomachs, lung, liver, and cecum in 38 of the animals. Two remaining animals were euthanatized because of chronic pododermatitis. Housing the animals in a pasture previously used by bovids and heavy rains with persistence of ground water pools in the pasture were contributing factors in the pathogenesis of this outbreak. 相似文献