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51.
Monoclonal antibodies to bovine viral diarrhea virus: cross-reactivities to field isolates and hog cholera virus strains. 总被引:2,自引:1,他引:1
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Monoclonal antibodies to bovine viral diarrhea virus (BVDV) were examined for binding with a large number of North American BVDV isolates and eight strains of the serologically related pestivirus, hog cholera virus (HCV). No single BVDV monoclonal antibody reacted with all BVDV isolates. The most cross-reactive monoclonal antibody was an anti-p80/p125 antibody which showed a positive reaction with 173 of 180 (96%) North American isolates. From a fewer number of isolates tested, one anti-gp53 monoclonal antibody also showed a high cross-reactivity (94%). All BVDV isolates showed a positive reaction with at least one of the seven monoclonal antibodies in the panel. Thus, the results indicated that a pool of these monoclonal antibodies may be used in place of polyclonal antisera for the detection of BVDV contamination of cell lines or for virus isolation. For HCV, all three anti-p80/p125 monoclonal antibodies reacted positively with all eight virus strains. In contrast, none of the anti-gp53 monoclonal antibodies were reactive to HCV strains. Thus, the anti-gp53 monoclonal antibodies may be useful for distinguishing between usually innocuous BVDV infections and the highly significant HCV infections in swine for foreign animal disease surveillance. 相似文献
52.
Comparative effects of mu and kappa opiate agonists on the cecocolic motility in the pony.
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The electrical and mechanical activity of the large intestine and its response to administration of opiate mu and kappa agonists were assessed from electrodes and inductograph coils chronically implanted on the cecocolic segment in six ponies given a diet of hay and concentrates. Before the drugs were given, migrating complexes propagating from the cecum into the colon occurred at the rate of 1.5 to 16/hour. During this propulsive activity, the cecocolic sphincter opened and closed allowing the outflow of cecal contents and preventing the backflow of colic contents. Each pony was used as its own control and was given fentanyl (0.01 and 0.05 mg/kg of body weight, IV) and U50488H (0.1 and 0.5 mg/kg, IV) at weekly intervals. The mu agonist fentanyl elicited a marked phase of inhibition of the propulsive activity and a closure of the cecocolic sphincter that lasted one to two hours depending on the dose. The kappa agonist U50488H induced an inhibition of the short spiking activity, i.e. of the resting muscle tone. It did not disturb the occurrence of migrating complexes nor that of the openings of the cecocolic sphincter. These kappa compounds may be drugs of choice to alleviate visceral pain in colic stases without inducing delay of transit unlike mu compounds. 相似文献
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葡萄二倍体与四倍体品种间杂交胚珠的离体培养 总被引:12,自引:2,他引:10
为了克服葡萄二倍体品种与四倍体品种之间的交配障碍,进行了杂交胚珠的离体培养研究。发芽胚珠的取样时期集中于授粉后35~75d,其中多数以二倍体作母本的组合在授粉后55d取样的发芽率最高,以四倍体作母本的组合授粉后70d取样的发芽率最高。基本培养模式为:发育培养基-(剥胚)萌发培养基-生长培养基。用二倍体品种作母本时,所有组合均获得了培养苗,在1号培养基上培养的胚珠发芽率高于2号培养基。反交对培养基的要求有相反趋势,其中2个组合未得到培养苗。授粉后60d,直接剥出杂种胚进行培养,得到了最高发芽率。综合分析初步认为,用二倍体品种作母本比用四倍体品种作母本容易获得杂交后代。 相似文献
56.
Pharmacokinetic evaluation of ceftiofur in serum, tissue chamber fluid and bronchial secretions from healthy beef-bred calves.
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S L Halstead R D Walker J C Baker R E Holland G E Stein J G Hauptman 《Canadian journal of veterinary research》1992,56(4):269-274
Ceftiofur is a new broad spectrum cephalosporin marketed for the treatment of acute bovine respiratory disease. In this investigation ceftiofur was administered by intramuscular injection, at 24 h intervals, to healthy beef-bred calves for four days at dosages of 2.2 and 4.4 mg/kg of body weight, with 4 wk intervals between dosing regimens. Serum, tissue chamber fluid (TCF), and bronchial secretion (BS) concentrations of ceftiofur were measured by microbiological assay after the first and fourth dose of each dosing regimen. Peak serum concentrations (Cmax) of 8.8 micrograms/mL and 17.3 micrograms/mL were obtained approximately 2 h (Tmax), the time of mean peak concentration) after single injections of 2.2 mg/kg and 4.4 mg/kg, respectively. The Cmax was increased approximately twofold following multiple doses of 2.2 mg/kg (Cmax = 13.1 micrograms/mL) and 4.4 mg/kg (Cmax = 24.1 micrograms/mL). Ceftiofur accumulated slowly into TCF and peak concentrations were found to be approximately 14% of those observed in serum after the first dose and approximately 24% after multiple dosing. Concentrations of ceftiofur in BS were obtained rapidly with peak concentrations reaching 45% of the serum Cmax after the first dose. After multiple dosing the Cmax for BS was approximately 25% of the serum Cmax. This study found that both the 2.2 mg/kg and 4.4 mg/kg dosing regimens resulted in continuous serum, TCF and BS concentrations of ceftiofur that exceeded the minimal concentration required to inhibit the bacteria most frequently isolated from calves with acute bovine respiratory disease. 相似文献
57.
越桔对长白山区酸性土壤的适应性 总被引:15,自引:1,他引:14
在长白山区3种类型的酸性土壤上栽培越桔,研究结果表明,`北空'、`美登'、`斯卫克'在暗棕色森林土上生长结果较好,树体营养关系合理;在草甸沼泽土上3个种生长结果均较差,`北空'在水湿地潜育土上生长旺盛,但产量低,综合分析认为,矮丛越桔类型品种对我国长白山区酸性土壤条件的适应性较强,半高丛越桔则适应性差,针对长白山不同类型的土壤,以美登最为适宜暗棕色森林土栽培,斯卫克次之;水湿地潜育土可栽培美登品种 相似文献
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