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81.
De Decker S Gielen IM Duchateau L Saunders JH van Bree HJ Polis I Van Ham LM 《American journal of veterinary research》2011,72(11):1496-1504
82.
Hänel I Hotzel H Müller W Tomaso H 《Berliner und Münchener tier?rztliche Wochenschrift》2011,124(5-6):198-202
Campylobacter fetus subsp. venerealis is the causative agent of bovine genital campylobacteriosis and is transmitted by asymptomatic carrier bulls via contaminated semen during artificial insemination. The aim of the present study was to determine the in vitro susceptibility of Campylobacter fetus subsp. venerealis isolated from bovine specimens in the years from 2000 to 2009 in Germany to antibiotics generally used in semen treatment. The susceptibilities of 50 strains to spectinomycin (10 microg), gentamicin (10 microg), streptomycin (25 microg), penicillin (10 microg), lincomycin (10 microg), ciprofloxacin (5 microg), erythromycin (30 microg) and tetracycline (30 microg) were determined using a disk diffusion susceptibility test. All strains were susceptible to gentamicin. A considerably reduced susceptibility to one or more antimicrobial agents was detected in seven out of 50 isolates (14%) with the most frequent reduction in susceptibility to lincomycin and spectinomycin. Furthermore, strains with reduced susceptibility to more than one antimicrobial agent were always associated with reduced susceptibility to lincomycin. It is recommended to determine the antimicrobial susceptibility of Campylobacter fetus subsp. venerealis isolates in order to evaluate the efficacy of the generally used antibiotic treatment of bull semen and to detect possible resistances. 相似文献
83.
Snorre Stuen Lise Gr?va Erik G Granquist Karin Sandstedt Ingrid Olesen H?vard Steinshamn 《Acta veterinaria Scandinavica》2011,53(1):8
Background
It has been questioned if the old native Norwegian sheep breed, Old Norse Sheep (also called Norwegian Feral Sheep), normally distributed on coastal areas where ticks are abundant, is more protected against tick-borne infections than other Norwegian breeds due to a continuously high selection pressure on pasture. The aim of the present study was to test this hypothesis in an experimental infection study.Methods
Five-months-old lambs of two Norwegian sheep breeds, Norwegian White (NW) sheep and Old Norse (ON) sheep, were experimentally infected with a 16S rRNA genetic variant of Anaplasma phagocytophilum (similar to GenBank accession number ). The experiment was repeated for two subsequent years, 2008 and 2009, with the use of 16 lambs of each breed annually. Ten lambs of each breed were inoculated intravenously each year with 0.4 ml A. phagocytophilum-infected blood containing approximately 0.5 × 106 infected neutrophils/ml. Six lambs of each breed were used as uninfected controls. Half of the primary inoculated lambs in each breed were re-challenged with the same infectious dose at nine (2008) and twelve (2009) weeks after the first challenge. The clinical, haematological and serological responses to A. phagocytophilum infection were compared in the two sheep breeds. M73220Results
The present study indicates a difference in fever response and infection rate between breeds of Norwegian sheep after experimental infection with A. phagocytophilum.Conclusion
Although clinical response seems to be less in ON-lambs compared to NW-lambs, further studies including more animals are needed to evaluate if the ON-breed is more protected against tick-borne infections than other Norwegian breeds. 相似文献84.
Live L Nesse Kristin Berg Lene K Vestby Ingrid Olsaker Berit Dj?nne 《Acta veterinaria Scandinavica》2011,53(1):44
Background
In Gram-negative bacteria, the most commonly studied quorum sensing signals are the N-acylhomoserine lactones (AHLs). In Salmonella, AHLs are recognized by SdiA, which is believed to be a sensor of AHLs produced by other bacteria, since Salmonella does not produce AHLs itself. It has been speculated that AHLs produced by the gastrointestinal flora may influence the regulation of virulence traits in Salmonella. The aim of the present work was to study the effect of AHLs on epithelial cell invasion by Salmonella in vitro.Methods
Invasion by Salmonella enterica subspecies enterica serovar Typhimurium (S. Typhimurium) strain and its isogenc sdiA mutant was studied using a conventional gentamycin invasion assay with HEp-2 cells at 37°C. Gene expression was studied using a semi-quantitative PCR.Results
The S. Typhimurium strain, but not its isogenic sdiA mutant, displayed increased in vitro invasion after addition of both N-hexanoyl-DL-homoserine lactone (C6-AHL) and N-octanoyl-DL-homoserine lactone (C8-AHL). Increased expression of two of the genes in the SdiA regulon (rck and srgE) was observed in the wild type strain, but not in the sdiA mutant.Conclusions
The results from the present study show that S. Typhimurium can respond to two different AHL quorum sensing signals (C6-AHL and C8-AHL) with increased cell invasion at 37°C in vitro, and that this response most likely is sdiA mediated. These results indicate that if AHLs are present in the intestinal environment, they may increase the invasiveness of Salmonella. 相似文献85.
Calfhood diseases have a major impact on the economic viability of cattle operations. A three part review series has been developed focusing on calf health from birth to weaning. In this paper, the last of the three part series, we review disease prevention and management with particular reference to pneumonia, focusing primarily on the pre-weaned calf. Pneumonia in recently weaned suckler calves is also considered, where the key risk factors are related to the time of weaning. Weaning of the suckler calf is often combined with additional stressors including a change in nutrition, environmental change, transport and painful husbandry procedures (castration, dehorning). The reduction of the cumulative effects of these multiple stressors around the time of weaning together with vaccination programmes (preconditioning) can reduce subsequent morbidity and mortality in the feedlot. In most studies, calves housed individually and calves housed outdoors with shelter, are associated with decreased risk of disease. Even though it poses greater management challenges, successful group housing of calves is possible. Special emphasis should be given to equal age groups and to keeping groups stable once they are formed. The management of pneumonia in calves is reliant on a sound understanding of aetiology, relevant risk factors, and of effective approaches to diagnosis and treatment. Early signs of pneumonia include increased respiratory rate and fever, followed by depression. The single most important factor determining the success of therapy in calves with pneumonia is early onset of treatment, and subsequent adequate duration of treatment. The efficacy and economical viability of vaccination against respiratory disease in calves remains unclear. 相似文献
86.
87.
Adrian Boswood Sonya G. Gordon Jens Häggström Martin Vanselow Gerhard Wess Rebecca L. Stepien Mark A. Oyama Bruce W. Keene John Bonagura Kristin A. MacDonald Mark Patteson Sarah Smith Philip R. Fox Karen Sanderson Richard Woolley Viktor Szatmári Pierre Menaut Whitney M. Church M. Lynne O'Sullivan Jean-Philippe Jaudon Jan-Gerd Kresken John Rush Kirstie A. Barrett Steven L. Rosenthal Ashley B. Saunders Ingrid Ljungvall Michael Deinert Eric Bomassi Amara H. Estrada Maria J. Fernandez Del Palacio N. Sydney Moise Jonathan A. Abbott Yoko Fujii Alan Spier Michael W. Luethy Roberto A. Santilli Masami Uechi Anna Tidholm Christoph Schummer Philip Watson 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(3):1108-1118
88.
Peeraporn Varinthra Shun-Ping Huang Supin Chompoopong Zhi-Hong Wen Ingrid Y. Liu 《Marine drugs》2021,19(1)
Age-related macular degeneration (AMD) is a progressive eye disease that causes irreversible impairment of central vision, and effective treatment is not yet available. Extracellular accumulation of amyloid-beta (Aβ) in drusen that lie under the retinal pigment epithelium (RPE) has been reported as one of the early signs of AMD and was found in more than 60% of Alzheimer’s disease (AD) patients. Extracellular deposition of Aβ can induce the expression of inflammatory cytokines such as IL-1β, TNF-α, COX-2, and iNOS in RPE cells. Thus, finding a compound that can effectively reduce the inflammatory response may help the treatment of AMD. In this research, we investigated the anti-inflammatory effect of the coral-derived compound 4-(phenylsulfanyl) butan-2-one (4-PSB-2) on Aβ1-42 oligomer (oAβ1-42) added to the human adult retinal pigment epithelial cell line (ARPE-19). Our results demonstrated that 4-PSB-2 can decrease the elevated expressions of TNF-α, COX-2, and iNOS via NF-κB signaling in ARPE-19 cells treated with oAβ1-42 without causing any cytotoxicity or notable side effects. This study suggests that 4-PSB-2 is a promising drug candidate for attenuation of AMD. 相似文献
89.
Evaluation of DRAINMOD using saturated hydraulic conductivity estimated by a pedotransfer function model 总被引:1,自引:0,他引:1
Direct measurement of soil saturated hydraulic conductivity (Ks) is time-consuming and therefore costly. The ROSETTA pedotransfer function model is able to estimate Ks from soil textural data, bulk density and one or two water retention points. This study evaluated the feasibility of running the DRAINMOD field-scale hydrological model with Ks input produced using ROSETTA. A hierarchical approach was adopted to estimate Ks using ROSETTA, with four limited-more extended sets of soil information used as inputs: USDA textural class (H1); texture (H2); texture and bulk density (H3); texture, bulk density, water retention at −33 kPa (θ33 kPa) and −1500 kPa (θ1500 kPa) (H4). ROSETTA-estimated Ks values from these four groups (H1-H4) were used in DRAINMOD to simulate drain outflows during a 4-year period from a conventional drainage plot (CD) and two controlled drainage plots (CWT1 and CWT2) located in south-east Sweden. The DRAINMOD results using ROSETTA-estimated Ks values were compared with observed values and with model results using laboratory-measured Ks values (H0). Deviations in simulated drainage outflow (D), infiltration (F) and evapotranspiration (ET) resulting from the use of ROSETTA-estimated rather than laboratory-measured Ks values were evaluated. During the study period, statistical comparisons showed good agreement on a monthly basis between observed and DRAINMOD-simulated drainage rates using five soil datasets (H0, H1, H2, H3 and H4). The monthly mean absolute error (MAE) ranged from 0.57 to 0.82 cm for CD, 0.38 to 0.41 cm for CWT1, and 0.15 to 0.22 cm for CWT2. On a monthly basis, the modified coefficient efficiency (E′) values were in the range of 0.62 to 0.74 for CD, 0.72 to 0.74 for CWT1, and 0.79 to 0.86 for CWT2. The modified index of agreement (d′) for monthly predictions ranged from 0.80 to 0.86 cm for CD, 0.87 to 0.88 cm for CWT1, and 0.89 to 0.93 cm for CWT2. The absolute values of the percent-normalised error (NE) on an overall basis when using ROSETTA-estimated rather than laboratory-measured Ks values were less than 3% in E, less than 1% in F, and less than 15% in D. The results suggest that ROSETTA-estimated Ks values can be used in DRAINMOD to simulate drainage outflows as accurately as laboratory-measured Ks values (H0) in coarse-textured soils. 相似文献
90.
Ingrid Allgoewer DVM Susanne Lucas DVM Stephan Albert Schmitz MD 《Veterinary radiology & ultrasound》2000,41(5):413-418
The magnetic resonance imaging appearance of the feline middle ear is described in three healthy cats and in five cats with middle ear disease. Owing to the good spatial resolution, multiplanar slice orientation as well as display high contrast resolution of soft tissue, in particular fluids, MR imaging was helpful prior to surgery. It is superior to radiography which failed to allow identification of the abnormality in two of our five cats. MR imaging for middle ear disease should include dorsal and transverse plane images using T1- and T2-weighted sequences. In the presence of a mass within the bulla or the external ear canal application of contrast medium is helpful. 相似文献