全文获取类型
收费全文 | 673篇 |
免费 | 36篇 |
专业分类
林业 | 31篇 |
农学 | 37篇 |
基础科学 | 1篇 |
127篇 | |
综合类 | 25篇 |
农作物 | 128篇 |
水产渔业 | 51篇 |
畜牧兽医 | 266篇 |
园艺 | 9篇 |
植物保护 | 34篇 |
出版年
2023年 | 3篇 |
2022年 | 11篇 |
2021年 | 15篇 |
2020年 | 13篇 |
2019年 | 19篇 |
2018年 | 23篇 |
2017年 | 26篇 |
2016年 | 27篇 |
2015年 | 35篇 |
2014年 | 33篇 |
2013年 | 54篇 |
2012年 | 55篇 |
2011年 | 74篇 |
2010年 | 34篇 |
2009年 | 29篇 |
2008年 | 53篇 |
2007年 | 51篇 |
2006年 | 38篇 |
2005年 | 29篇 |
2004年 | 26篇 |
2003年 | 16篇 |
2002年 | 21篇 |
2001年 | 11篇 |
2000年 | 6篇 |
1999年 | 4篇 |
1997年 | 1篇 |
1989年 | 2篇 |
排序方式: 共有709条查询结果,搜索用时 31 毫秒
151.
152.
Kim CH Lillehoj HS Bliss TW Keeler CL Hong YH Park DW Yamage M Min W Lillehoj EP 《Veterinary immunology and immunopathology》2008,124(3-4):341-354
153.
Fazlurrahman Khan Min-Gyun Kang Du-Min Jo Pathum Chandika Won-Kyo Jung Hyun Wook Kang Young-Mog Kim 《Marine drugs》2021,19(11)
With the advancement of nanotechnology, several nanoparticles have been synthesized as antimicrobial agents by utilizing biologically derived materials. In most cases, the materials used for the synthesis of nanoparticles from natural sources are extracts. Natural extracts contain a wide range of bioactive components, making it difficult to pinpoint the exact component responsible for nanoparticle synthesis. Furthermore, the bioactive component present in the extract changes according to numerous environmental factors. As a result, the current work intended to synthesize gold (AuNPs) and zinc oxide (ZnONPs) nanoparticles using pure phloroglucinol (PG). The synthesized PG-AuNPs and PG-ZnONPs were characterized using a UV–Vis absorption spectrophotometer, FTIR, DLS, FE-TEM, zeta potential, EDS, and energy-dispersive X-ray diffraction. The characterized PG-AuNPs and PG-ZnONPs have been employed to combat the pathogenesis of Pseudomonas aeruginosa. P. aeruginosa is recognized as one of the most prevalent pathogens responsible for the common cause of nosocomial infection in humans. Antimicrobial resistance in P. aeruginosa has been linked to the development of recalcitrant phenotypic characteristics, such as biofilm, which has been identified as one of the major obstacles to antimicrobial therapy. Furthermore, P. aeruginosa generates various virulence factors that are a major cause of chronic infection. These PG-AuNPs and PG-ZnONPs significantly inhibit early stage biofilm and eradicate mature biofilm. Furthermore, these NPs reduce P. aeruginosa virulence factors such as pyoverdine, pyocyanin, protease, rhamnolipid, and hemolytic capabilities. In addition, these NPs significantly reduce P. aeruginosa swarming, swimming, and twitching motility. PG-AuNPs and PG-ZnONPs can be used as control agents for infections caused by the biofilm-forming human pathogenic bacterium P. aeruginosa. 相似文献
154.
Hyeong Rok Yun Sang Woo Ahn Bomin Seol Elena A. Vasileva Natalia P. Mishchenko Sergey A. Fedoreyev Valentin A. Stonik Jin Han Kyung Soo Ko Byoung Doo Rhee Jung Eun Seol Hyoung Kyu Kim 《Marine drugs》2021,19(11)
Atopic dermatitis (AD) is a chronic inflammatory skin disease in which skin barrier dysfunction leads to dryness, pruritus, and erythematous lesions. AD is triggered by immune imbalance and oxidative stress. Echinochrome A (Ech A), a natural pigment isolated from sea urchins, exerts antioxidant and beneficial effects in various inflammatory disease models. In the present study, we tested whether Ech A treatment alleviated AD-like skin lesions. We examined the anti-inflammatory effect of Ech A on 2,4-dinitrochlorobenzene (DNCB)-induced AD-like lesions in an NC/Nga mouse model. AD-like skin symptoms were induced by treatment with 1% DNCB for 1 week and 0.4% DNCB for 5 weeks in NC/Nga mice. The results showed that Ech A alleviated AD clinical symptoms, such as edema, erythema, and dryness. Treatment with Ech A induced the recovery of epidermis skin lesions as observed histologically. Tewameter® and Corneometer® measurements indicated that Ech A treatment reduced transepidermal water loss and improved stratum corneum hydration, respectively. Ech A treatment also inhibited inflammatory-response-induced mast cell infiltration in AD-like skin lesions and suppressed the expression of proinflammatory cytokines, such as interferon-γ, interleukin-4, and interleukin-13. Collectively, these results suggest that Ech A may be beneficial for treating AD owing to its anti-inflammatory effects. 相似文献
155.
Jeong‐Hyun Seo Ki‐Seung Kim Jong‐Min Ko Man‐Soo Choi Beom‐Kyu Kang Soon‐Wook Kwon Tae‐Hwan Jun 《Plant Breeding》2019,138(1):95-104
Soybean seed protein and oil concentrations are important traits that directly affect the quality of soyfoods. Many studies and breeding programmes have been conducted to find major quantitative trait loci (QTL) that regulate protein and oil concentrations and to develop soybean cultivars with high protein and/or oil content. The purpose of this study was to identify these QTL using a selected breeding population. The population was tested in field conditions over a period of 3 years. Seed protein and oil concentrations were measured each year. Single‐nucleotide polymorphisms (SNPs) were used to construct genetic map using a 180K SoyaSNP array, which identified 1,570 SNPs. We identified 12 QTL for seed protein, 11 for seed oil concentration and four for both traits. Among these, 17 QTL were closely mapped to previously reported QTL, whereas ten sites were novel. Several QTL were detected across at least two experimental years. These loci are good candidate QTL for optimal seed protein and oil concentrations. Our results demonstrate that favourable target QTL can be successfully identified using selected breeding populations. 相似文献
156.
Rauf Muhammad Yoon Hyemyeong Lee Sukyeung Shin Myoung-Jae Ko Ho-Cheol Lee Myung-Chul Oh Sejong Hyun Do-Yoon Hur Onsook Choi Yu Mi 《Journal of Crop Science and Biotechnology》2019,22(5):495-507
Journal of Crop Science and Biotechnology - Oat (Avena sativa L.) is one of the world’s most important cereal crops, owing to its use as an important source of essential nutrients for both... 相似文献
157.
Young Chul Kim Woo Ju Kwon Joon Gyu Min Kwang Il Kim Hyun Do Jeong 《Journal of fish diseases》2019,42(4):519-531
We determined the complete genomic RNA sequence of a new type of betanodavirus Korea shellfish nervous necrosis virus (KSNNV) isolated from shellfish. Compared with other isolates representing four genotypes of betanodaviruses, the identity of the whole nucleotide sequence of the virus was in the range of 76%–83% with the presence of specific genetic motifs and formed a separate new branch in the phylogenetic analysis. In pathogenic analysis by immersion method, KSNNV‐KOR1 shows 100% cumulative mortality like SFRG10/2012BGGa1 (RGNNV) in newly hatched sevenband grouper and mandarin fish, which is clearly different from those found in negative control groups. There were no significant differences in increasing rates of mortality and viral intra‐tissue concentration of larval fishes infected with KSNNV‐KOR1 at both 20 and 25°C water temperature. Histopathological examination of each fish species in the moribund stage revealed the presence of clear vacuoles in both brain and retinal tissues similar to typical histopathology features of RGNNV. In the present study, we first report a new betanodavirus from shellfish as the aetiological agent of viral nervous necrosis disease in fish with complete genomic nucleotide sequence and pathogenic analysis. 相似文献
158.
ABSTRACT: As part of our study of the isolation of antihypertensive agents derived from natural marine products, the bioactivity of 10 edible Korean seaweeds were screened by angiotensin converting enzyme (ACE) inhibitory and peroxynitrite assays. Among the crude extracts of selected seaweeds, including five Phaeophyta ( Ecklonia stolonifera , E. cava , Pelvetia siliquosa , Hizikia fusiforme , and Undaria pinnatifida ), four Rhodophyta ( Gigartina tenella , Gelidium amansii , Chondria crassicaulis , and Porphyra tenera ) and one Chlorophyta ( Capsosiphon fulvescens ), the ethanol extracts of E. stolonifera , E. cava , P. siliquosa , U. pinnatifida , and G. tenella exhibited significant inhibitory properties against ACE at more than 50% inhibition at a concentration of 163.93 µg/mL. Phloroglucinol 1 , eckstolonol 2 , eckol 3 , phlorofucofuroeckol A 4 , and dieckol 5 had been isolated previously, and triphlorethol-A 6 and fucosterol 7 were isolated for the first time from E. stolonifera. Also, the ACE inhibitory and peroxynitrite scavenging properties of phlorotannins 1–6 were evaluated, along with fucosterol 7 obtained from E. stolonifera . Among profound peroxynitrite scavenging compounds 1–6 , phlorotannins 3 , 4 and 5 were also determined to manifest marked inhibitory activity against ACE, with 50% inhibition concentration (IC50 ) values of 70.82 ± 0.25, 12.74 ± 0.15, and 34.25 ± 3.56 µM, respectively. 相似文献
159.
160.
Kim Hyun Kyung Kim Young Ho Kim Yun Ji Park Hyun Jin Lee Nam Hyouck 《Fisheries Science》2012,78(2):485-490
In this study we investigated the effects of ultrasonic wave treatment on the extraction yield of acid-soluble collagen from
sea bass skins. Two extraction methods were compared: a 24 h acid treatment using 0.5 M acetic acid (1:200 sample/acid, w/v)
and an extraction using ultrasonic treatment after the addition of a 0.5 M acetic acid solution. The results indicated that
the extraction yield of collagen increased with the ultrasonic treatment, with the extraction rate increasing rapidly at higher
amplitudes of ultrasonic treatment. The subunit compositions of the collagen extracted by ultrasonic treatment were analyzed
by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, which revealed that the α1(α3), α2, and β chains of collagen
were present early in the ultrasonic treatment. An unknown component, believed to be a product of collagen degradation induced
by the ultrasonic treatment, was detected only after a longer treatment time. The component extracted by the ultrasonic treatment
was determined to be collagen based on the finding that there were no changes in the main components of collagen, specifically,
the α1(α3), α2, and β chains, following pepsin treatment. 相似文献