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41.
X Y Wang M L Liao T H Hung P A Seib 《Journal of the Association of Official Analytical Chemists》1988,71(6):1158-1161
An accurate method was devised to assay L-ascorbic 2-polyphosphate esters (AsPP) in fish feed by phosphatase digestion followed by determination of the released L-ascorbic acid (AsA). Compressed yeast and dithiothreitol are added to the phosphatase reaction mixture to give 95-100% recovery of AsA, which is quantitated by reverse-phase liquid chromatography (LC) with electrochemical detection. Chromatograms of all feed digests showed baseline resolution of AsA. In 3 feeds, to which 75-125 ppm AsA equivalents in the form of AsPP were added, the assay procedure gave 98-100% recovery of AsA. 相似文献
42.
M. B. Barry J. L. Pham B. Courtois C. Billot N. Ahmadi 《Genetic Resources and Crop Evolution》2007,54(8):1675-1690
Rice genetic diversity partitioning between farms, varieties and, within-variety diversity, were analysed in two villages
of Maritime Guinea with contrasted agroecological conditions. One thousand and two hundred individual plants belonging to
45 accessions collected in eight farms were genotyped using 10 SSR markers. The molecular variance was evenly shared between
and within accessions, while the farm effect was almost nil. Local varieties had a multi-line genetic structure. The number
of multilocus genotypes was proportional to the utilisation rate of the variety in the village. The F
ST values between different accessions of each variety were significant which indicated low genetic consistency in the variety
names. This varietal structure could mainly be explained by the migration phenomenon and the high varietal turnover. Compared
to allelic diversity, multilocus genotypic diversity seemed to be the most suitable indicator of the quantitative distribution
of diversity at different management scales (accession, farm and village). The within- and between-farm F
ST values were in the same order of magnitude. The within-farm diversity was not farm-specific but quantitatively high, i.e.
up to 50% of the total genotypic diversity of a given village. Given the relative importance of the within-variety diversity,
the in situ approach stands out as the most effective solution. As farms do not host specific diversity the in situ approach
could be implemented by working with a small number of farms. 相似文献
43.
Yung-Tse Hung W. Wesley Eckenfelder Jr. Tom D. Reynolds 《Water, air, and soil pollution》1978,10(2):175-185
The laboratory bench-scale experiments were conducted to investigate the effects of varying degrees of waste treatment and the flow conditions in the receiving waters on the deoxygenation rate constant (k), and ultimate biochemical oxygen demand (L), and the total O2 utilisation of the receiving waters. Industrial wastewaters were collected from 10 major industrial plants located along the Houston Ship Channel in Texas. Two different degrees of treatment for industrial wastewaters were studied. Three different flow conditions studied were the high, the average and the low flow in the Houston Ship Channel. The k values increased for both further treatment and decreased flow in the reactor runs. The L values were higher for the average flow conditions than for the low flow conditions. The total O2 utilization was found to increase with a decrease in the flow of the receiving waters. 相似文献
44.
Junli Hu Fuyong Wu Shengchun Wu Junhua Wang Xiangui Lin Ming Hung Wong 《Journal of Soils and Sediments》2016,16(9):2195-2202
Purpose
Fruiting vegetables are generally considered to be safer than other vegetables for planting on cadmium (Cd)-contaminated farms. However, the risk of transferring Cd that has accumulated in the stems and leaves of fruiting vegetables is a major issue encountered with the usage of such non-edible parts. The objective of this study was to resolve the contribution of arbuscular mycorrhizal (AM) fungi to the production of low-Cd fruiting vegetables (focusing on the non-edible parts) on Cd-contaminated fields.Materials and methods
An 8-week pot experiment was conducted to investigate the acquisition and translocation of Cd by cucumber (Cucumis sativus L.) plants on an unsterilized Cd-contaminated (1.6 mg kg?1) soil in response to inoculation with the AM fungus, Funneliformis caledonium (Fc) or Glomus versiforme (Gv). Mycorrhizal colonization rates of cucumber roots were assessed. Dry biomass and Cd and phosphorus (P) concentrations in the cucumber shoots and roots were all measured. Soil pH, EC, total Cd, phytoavailable (DTPA-extractable) Cd, available P, and acid phosphatase activity were also tested.Results and discussion
Both Fc and Gv significantly increased (P?<?0.05) root mycorrhizal colonization rates and P acquisition efficiencies, and thus the total P acquisition and biomass of cucumber plants, whereas only Fc significantly increased (P?<?0.05) soil acid phosphatase activity and the available P concentration. Both Fc and Gv significantly increased (P?<?0.05) root to shoot P translocation factors, inducing significantly higher (P?<?0.05) shoot P concentrations and shoot/root biomass ratios. In contrast, both Fc and Gv significantly decreased (P?<?0.05) root and shoot Cd concentrations, resulting in significantly increased (P?<?0.05) P/Cd concentration ratios, whereas only Gv significantly decreased (P?<?0.05) the root Cd acquisition efficiency and increased (P?<?0.05) the root to shoot Cd translocation factor. Additionally, AM fungi also tended to decrease soil total and phytoavailable Cd concentrations by elevating plant total Cd acquisition and soil pH, respectively.Conclusions
Inoculation with AM fungi increased the P acquisition and biomass of cucumber plants, but decreased plant Cd concentrations by reducing the root Cd acquisition efficiency, and resulted in a tendency toward decreases in soil phytoavailable and total Cd concentrations via increases in soil pH and total Cd acquisition by cucumber plants, respectively. These results demonstrate the potential application of AM fungi for the production of fruiting vegetables with non-edible parts that contain low Cd levels on Cd-contaminated soils.45.
Mei-Hua Hung Arun A. Bhagwath Fo-Ting Shen Rekha P. Devasya Chiu-Chung Young 《Pedobiologia》2005,49(6):36-584
Root-nodulating bacteria were isolated and characterized from seven native shrubby legumes growing in Taiwan. Phenotypic characteristics measured included growth rates in various media, colony morphology, and tolerances to extremes of temperature, salt and pH. The isolates were very diverse phenotypically. Among the 83 isolates that were screened, the majority were fast-growing rhizobia. Twenty eight strains tolerated high concentration of salt (4.5% NaCl) and grew well between temperatures of 37 and 45 °C. The majority of the strains also tolerated extreme pH in their medium from 3.5 to 12. All strains formed nitrogen fixing nodules, and the highest activity was detected in the legume Hedysarum crinita L. PCR restriction fragment length polymorphism (PCR-RFLP) and sequencing of the small subunit ribosomal RNAs revealed that the majority of the isolates belonged to the genera Rhizobium, Bradyrhizobium and Agrobacterium. Only a single strain represented the genus Sinorhizobium. In addition, a strain related to Burkholderia from the β-class of the Proteobacteria (CC-CC-5) was found within nodules of the legume Catenaria caudatum. The study contributes to the understanding of symbiotic nitrogen fixation in selected wild legumes that are native to Taiwan and provides insights into the distribution of nodulating and nitrogen-fixing bacteria from other distinct lineages. 相似文献
46.
Antioxidant properties of crocin from Gardenia jasminoides Ellis and study of the reactions of crocin with linoleic acid and crocin with oxygen 总被引:6,自引:0,他引:6
Pham TQ Cormier F Farnworth E Tong VH Van Calsteren MR 《Journal of agricultural and food chemistry》2000,48(5):1455-1461
Crocin-a water soluble carotenoid-is found in the fruits of gardenia (Gardenia jasminoides Ellis) and in the stigmas of saffron (Crocus sativus Linne). For crocin purification, gardenia fruits are extracted with 50% acetone, followed by ether washing, ion exchange, and separation by preparative HPLC. Purified crocin with purity of >99.6% has an antioxidative activity at concentrations up to 40 ppm. At 20 ppm the antioxidative activity of crocin is comparable to that of BHA. The antioxidant property of crocin as evaluated by the thiocyanate method was better than with the thiobarbituric acid method. The adduct between the linoleic acid radical and crocin was detected by LC-MS. When crocin reacted with oxygen in the presence of FeSO(4), intermediates such as monohydroperoxides and dihydroperoxides of crocin were formed and detected by LC-MS. 相似文献
47.
Effects of mycorrhizal inoculation of upland rice on uptake kinetics of arsenate and arsenite 下载免费PDF全文
We assessed the effect of mycorrhizal inoculation on short‐term uptake kinetics of arsenate and arsenite by excised roots of upland rice (Oryza sativa L. cv. Zhonghan 221). A concentration of 0.01–0.05 mM arsenic (As) differentially affected the influx rates of both arsenate and arsenite into rice roots non‐inoculated or inoculated with Glomus mosseae and G. versiforme. While Vmax for arsenate uptake by non‐mycorrhizal roots was 1.02 µmol g?1 fresh weight h?1, it was reduced by a factor of 2.4 for mycorrhizal roots (about 0.42 µmol g?1 fresh weight h?1) in the high‐affinity uptake system. However, at high concentrations of 0.5–2.5 mM As only G. versiforme was able to reduce As influx. The results show that mycorrhizal effects on As uptake of upland rice are both concentration and species‐specific. 相似文献
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Tran Lanh Danh Phung Luc Duc Pham Dung Viet Pham Dong Duy Nishiyama Masateru Sasaki Atsushi Watanabe Toru 《Paddy and Water Environment》2019,17(3):507-513
Paddy and Water Environment - Two consecutive pilot-scale experiments were conducted to investigate the potential reuse of treated municipal wastewater (TWW) as an irrigation source for cultivating... 相似文献