Contaminant and formulation analysis of MSMA using high-pressure liquid chromatography — graphite furnace atomic absorption spectrophotometry

High-pressure liquid chromatography with an ion-exchange column combined with graphite furnace atomic absorption spectrometry was used to analyse commercial formulations of sodium hydrogen methylarsonate (MSMA). No arsenite or arsenate salts or dimethylarsinic acid were detected as contaminants in the formulations, and the MSMA concentrations were found to be in accordance with the concentrations given on the containers.     

黄河流域机采棉植株性状的筛选

为筛选适合黄河流域棉区机采的品种,对16份棉花材料的果枝始节高度、株高、产量构成因素、纤维品质指标进行系统调查、分析与研究。结果表明:适合进一步培育机采品种的材料为590032、中棉所49。     

河北省冬小麦田杂草群落特征

为了明确河北省冬小麦田杂草的群落组成和结构,采用倒置"W"取样法对河北省7个地区146块冬小麦田的杂草进行了调查和物种多样性测度。结果显示,河北省冬小麦田有61种杂草,隶属于21科53属,播娘蒿、打碗花、荠菜、麦瓶草和麦家公是河北省冬小麦田的优势杂草。保定地区麦田杂草群落的物种丰富度最大;沧州地区麦田杂草群落的Shannon-Wiener指数和Pielou指数最大;廊坊地区麦田杂草群落的Gleason指数、Shannon-Wiener指数和Pielou指数最小,优势草种比较突出。经聚类分析和主成分分析,河北省冬小麦田杂草群落分为3组:石家庄、保定、邢台和廊坊的麦田杂草群落中耐旱杂草的相对多度较高;沧州和衡水的麦田杂草群落中耐盐杂草的相对多度较高;邯郸的麦田杂草群落中喜湿杂草的相对多度较高。说明土壤的盐度和湿度是影响河北省冬小麦田杂草群落组成的主要生态因子。     

活体动物中大环内脂类残留检测微生物抑制法的建立

[目的]建立活体动物中大环内酯类残留的检测方法。[方法]采用微生物抑制方法,对藤黄微球菌CMCC 28001的选择性和特异性、样品提取方法、平板制备条件、方法检出限等技术参数进行研究。[结果]建立的方法可用于活体动物中红霉素、泰乐菌素、螺旋霉素、林可霉素、吉他霉素和克林霉素等6种大环内脂类药物的残留检测。当菌悬液添加浓度为10~(-3)倍稀释液(5.5×106CFU/m L)以及培养基PH值为7.5且培养温度为30℃时,可产生直径最大,边缘光滑、完整、清晰的抑菌圈。[结论]该方法具有良好的敏感性和重复性,成本低,操作简便,是色谱分析、免疫分析等其它方法的有益补充。     

窥探我国果农的合作之路

改革开放以来,我国果业迅猛发展,但产销体制很不完善,产品本身不等于商品,更不等于有了稳定畅销的渠道和市场份额。分散的个体果农户面对竞争的市场,犹如汪洋大海中的叶叶扁舟,显得无能为力。果农协会组织和引领分散果农走向市场,作为利益共享、风险共担的非盈利组织形式,在国外已经是成功的经验和模式,应成为我们之鉴。为振兴我国果业,合作化是我国个体果农的必由之路。果农协会应当实行完全民主化运作,避免“权力”的介入而使之变为“半官方化”、“机关化”和“官僚化”。政府应在制订政策条例、协助开拓市场、科技服务等方面发挥其作用。果农的教育和科技水平亟待提高,民主意识亟待培养和增强。政府在这些方面大有可为。     

触角电位(EAG)引导方法的探讨

在菜粉蝶(Artogeia rapae)同一触角上施行不同处理时,触角的EAG 反应变化很大:在体、端部完整的状态反应最强,剪去端部会在一定程度上降低反应性,离体则反应微弱。而且,在体触角在2小时内反应性下降不很大,而绝大多数离体触角都在试验开始后60分钟内失活。     

Cyclooxygenase ‐2 Expression in Mammary Tumors in Dogs and its Correlation to Histologic and Biologic Behavior

Introduction:  Cyclooxygenase‐2 (Cox‐2) is the inducible form and the rate‐limiting enzyme, for conversion of arachidonic acid to prostaglandins. Cox‐2 overexpression, common in carcinomas, is associated with increased growth rate, resistance to apoptosis, angiogenesis, and overall, both local and distant aggressive behavior. Cox‐2 overexpression has been detected in human and canine mammary tumors (MTs). Histopathology of canine MT is not always predictive of biologic behavior, and anecdotally, only 50% of the malignant MTs are expected to metastasize. We hypothesize that Cox‐2 expression correlates with aggressive behavior.
Methods:  This retrospective study evaluated 48 bitches, presented for excision of MT between 2000 and 2003 at FMVZ de Botucatu‐UNESP, Brasil. Follow‐up varied from 18 months to 24 months and included physical examination and thoracic radiographs. Histopathologic examination was performed in all tumors, as well as in metastatic lesions when detected in the follow‐up period. Immunohistochemistry was used to detect expression of Cox‐2 in paraffin blocks (Rabbit polyclonal anti‐PGHS‐2. Oxford Biomedical). 10 adenomas, 10 carcinomas, 10 benign mixed tumors, 10 malignant mixed tumors and 8 cases of primary carcinomas and their metastatic lesions.
Results:  Expression of Cox‐2 varied among groups. Adenomas (32.1%), mixed benign tumors (38%), carcinomas (60.3%), malignant mixed tumors (65.8%), and metastatic carcinomas (81.25%) and their metastatic lesions (84.35%). Statistically significant differences (p < 0.05) were observed between the benign and malignant counterparts and between carcinomas and metastatic carcinomas.
Conclusions:  Cox‐2 expression correlates with both histologic and biologic behavior in mammary carcinomas, and may serve as a predictor of metastatic potential.     

Bovine herpesvirus type 1 (BHV-1) up-regulates telomerase activity in MDBK cells

The proliferative capacity of mammalian cells is regulated by telomerase, an enzyme uniquely specialised for telomeric DNA synthesis. The critical role of telomerase activation in tumor progression and maintenance has been well established in studies of cancer and of oncogenic transformation in cell culture. Experimental data suggest that telomerase activation has an important role in normal somatic cells, and that failure to activate sufficient telomerase also promotes disease. Evidence regarding the role of telomerase in the pathogenesis of several viruses including human immunodeficiency virus has led to an increased interest in the role of telomerase activity in other virus infections. In this research we evaluated the telomerase modulating activity of Bovine herpesvirus 1 (BHV-1) in MDBK cells. MDBK cells were infected at different multiplicity of infection with BHV-1 Cooper strain and telomerase activity at different times post-infection was measured by the TRAP assay. Our data indicate that BHV-1 significantly up-regulates telomerase activity at 3 and 6h post-infection decreasing after the 24h post-infection. Our data, showed that the effect was mediated by an immediate-early or early viral gene, and use of the protein translation inhibitor cycloheximide confirmed that an immediate early gene is primarily responsible.     

A true "triphasic" pattern: thoracolumbar spinal tumor in a young dog

An 8-month-old male Bernese Mountain Dog was referred with a history of hindlimb weakness that progressed to paresis on the right side. An intradural mass was detected in the spinal canal at the level of the 2nd and 3rd lumbar vertebrae. During surgical removal, 2 small fragments of the mass were prepared for cytologic examination by the squash technique. Cytologic examination revealed 3 different cell types: mesenchymal (stromal) cells, epithelial cells, and small undifferentiated hyperchromatic cells. On the basis of location and the triphasic cytologic pattern, a diagnosis of spinal nephroblastoma (thoracolumbar spinal tumor of young dogs) was made; histologic examination of the mass confirmed the cytologic diagnosis. To our knowledge, this is the first report of a triphasic pattern in a cytologic sample; recognizing this pattern is an important aid in reaching a definitive cytologic diagnosis.     

鄱阳湖平原土地利用变化对生态网络稳定性影响的模拟分析

土地利用变化是改变区域景观结构和生态特征的主要原因,也是影响区域生态稳定性的重要原因。以鄱阳湖平原地区为研究区,基于2000、2010和2020年的3期遥感影像数据,对鄱阳湖平原地区不同情景下2030年土地利用变化进行定量模拟,并采用连通鲁棒性方法对生态网络的稳定性进行分析。结果表明：1）鄱阳湖平原地区未来土地利用变化中耕地、建设用地面积变化最大,其中,城市快速增长情景（Fast Urban Growth, FUG）下的建设用地面积增长最多,较2020年增长了70.27%;耕地面积在3种未来情景中面积均有减少;林地面积在常规发展情景（Conventional Development Scenario, CDS）、FUG和生态保护情景（Ecological Conservation Scenario,ECS）下较2020年分别减少了6.90%、7.23%和6.70%。2）通过景观连通性评价得到鄱阳湖平原地区2030年CDS、FUG和ECS情景下的生态源地面积分别为2 464.2、2 666.72和3 141.88 km2;识别生态廊道169、255和299条;可以看出,ECS情景能保留更多的生态源地和生态廊道。3）3种未来模拟情景中,FUG下生态廊道\