陕西杨陵地区猪弓形虫病的血清学调查研究

应用间接血凝试验（ＩＨＡ）对采自杨陵区食品公司屠宰点、西北农业大学实验农场种猪场,西北农林科技大学兽医院的２１４份猪血样进行弓形虫抗体的定性检测,包括血清检测法（１７６份）,滤纸干血滴法（３８份）两种方法。结果检出阳性血样５份,阳性率为２．３４％。其中来自杨陵区食品公司屠宰点猪血样１３６份,全部用血清检测法测定结果,阳性５份,阳性率为３．６８％,来自西北农林科技大学农场种猪场血样７６份,３８份血清     

饮高碘水对肉仔鸡生产性能及组织器官碘含量的影响

试验用艾维茵肉仔鸡１２０只,随机分为４组,分别饮用每千克含碘量为１５ｍｇ,２５ｍｇ,４５ｍｇ和０ｍｇ的饮水,饮用３７ｄ。结果表明：不同的碘水平对肉鸡生产性能、器官发育均未产生不良影响。但高碘饮水组鸡的组织碘含量均显著高于对照组鸡。     

<Emphasis Type="Italic">Echinococcus granulosus</Emphasis>: Immunoprotection accompanyied by humoral and cytokine response against secondary hydatidosis in mice immunized with r<Emphasis Type="Italic">Eg.</Emphasis>myophilin

Objects:  

This study aimed to investigate the immunoprotection of the recombinant Eg.myophilin (rEg.myophilin) and tentatively analyze mechanism of this protection.     

Veterinary aspects of ecological monitoring: the natural history of emerging infectious diseases of humans,domestic animals and wildlife

Proliferation of disease pathogens capable of affecting humans, domestic livestock and wildlife increasingly threatens environmental security and biodiversity. Livestock and wild animals in proximity to human beings are often in the chain of transmission and infection. Globalization of industrial livestock production (especially poultry upon which so much of the burgeoning human population depends) often permits transcontinental disease spread. Rapidly expanding (and often illegal) international trade in wild and domestic animals and their products are increasingly involved in the emergence of new diseases that may have the ability to transmit among humans, livestock and wildlife. Rapidly increasing urbanization has led in many places to overcrowded townships that rely on “bushmeat” for sustenance and has contributed to the emergence of virulent zoonotic pathogens. The emergence and proliferation of pathogens are exacerbated by anthropogenic transformation of natural landscapes in order to increase agricultural and livestock production. This paper posits that data gathered by veterinary ecologists should be interpreted and used by other disciplines. The importance of a thorough knowledge of the “natural history” (ecology) of the disease agent and its human, domestic and wild hosts is stressed.     

一起猪球虫病感染诊断与防治措施

本文通过对湖南省岳阳市君山区某养猪场的10kg以下的发病仔猪进行临床症状观察和实验室诊断,确诊为猪球虫病。通过采用内服妥曲株利进行治疗,有效控制了病情。     

黄芩注射液的制备工艺及质量标准的初步研究

目的:研制以黄芩和柴胡为主药的注射剂,并建立质量控制标准。方法:以传统的水提醇沉法制备该注射剂,采用薄层色谱法对黄芩甙进行鉴定,并用紫外分光光度法测定黄芩甙的含量。结果:采用水提醇沉法制备得到的该注射剂符合药典关于注射剂的各项检查。实验结果表明:黄芩苷含量在1.6μg/mL～8.0μg/mL与吸光度成良好的线性关系,相关系数0.9996,平均回收率99.4%,相对标准偏差为0.6%(n=5)。黄芩甙的薄层色谱鉴别,薄层色谱斑点清晰、空白无干扰,重现性好。结论:该制剂生产工艺简单,质量标准简单易行,产品质量容易控制。     

Socio-economic factors influencing control of vector-borne diseases in the pastoralist system of south western Uganda

Research in control of tick-borne diseases and trypanosomosis, and their vectors, namely, ticks and tsetse flies respectively, has been on going for decades. However, very little attention has been paid to the socio-economic factors that are likely to influence the outcome of the interventions in the control of these diseases. Thus, this study was designed to investigate these factors, mainly the intra-household factors influencing decision-making in the control of Vector-borne diseases in the pastoralist areas of Uganda. These factors included: indigenous technical knowledge, household economic factors, and gender. Both qualitative and quantitative methods were used in the collection and analysis of data. The tools used for data collection included among others, participatory learning and action (PLA), and Case studies. The findings included the following: In pastoralist households, a big proportion of the household budget was allocated to vector-borne diseases control. In the male-headed households, men dominated decision-making on vector-borne diseases control, although the goals and priorities of men and women in these households were not the same. Also, vector-borne disease control was predominantly by use of modern veterinary drugs, and pastoralists treated sick cattle by themselves even in situations where there were veterinary personnel.     

Mitochondrial dysfunction influences apoptosis and autophagy in porcine parthenotes developing in vitro

Mitochondria are important regulators of both apoptosis and autophagy. One of the triggers for mitochondrial-mediated apoptosis is the production of reactive oxygen species (ROS), which include hydrogen peroxide, superoxide, hydroxyl radical, nitric oxide and peroxynitrite. Recently, several studies have indicated that ROS may also be involved in the induction of autophagy. In the present study, we used H(2)O(2) to induce mitochondrial stress, examined apoptotic- and autophagic-related gene expression and observed LC3 protein (autophagosome presence marker) expression in porcine parthenotes developing in vitro. In porcine four-cell parthenotes cultured for 5 days in NCSU37 medium containing 0.4% BSA, the developmental rate and mitochondrial distribution did not differ from that of the group supplemented with 100 μM H(2)O(2) but was significantly decreased in the group supplemented with 500 μM H(2)O(2) (P<0.05). Transmission electron microscopy (TEM) indicated that whereas normal shaped mitochondria were observed in blastocysts from the control group, abnormal mitochondria (mitophagy) and autophagic vacuoles were observed in blastocysts from the group that received 500 μM H(2)O(2). Furthermore, addition of H(2)O(2) (100 μM and 500 μM) decreased cell numbers (P<0.05) and increased both apoptosis (P<0.05) and LC3 protein expression in the blastocysts. Real-time RT-PCR showed that H(2)O(2) significantly decreased mRNA expression of anti-apoptotic gene Bcl-xL but increased pro-apoptotic genes, Caspase 3 (Casp3) and Bak, and autophagy-related genes, microtubule-associated protein 1 light chain 3 (Map1lc3b) and lysosomal-associated membrane protein 2 (Lamp2). However, the addition of H(2)O(2) had no effect on mRNA expression levels in nuclear DNA-encoded mitochondrial-related genes, cytochrome oxidase (Cox) 5a, Cox5b and Cox6b1, in blastocysts. These results suggest that H(2)O(2) leads to mitochondrial dysfunction that results in apoptosis and autophagy, which is possibly related to porcine early embryo development.