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611.
Renal function, insulin secretion, and glucose tolerance in mild streptozotocin diabetes in the dog.
J J Kaneko D Mattheeuws R P Rottiers A Vermeulen 《American journal of veterinary research》1978,39(5):807-809
A mild, stable diabetes mellitus was induced in dogs, using a small (20 mg/kg of body weight) dose of streptozotocin in a standard volume of 200 ml infused in 15 minutes. The stability of the diabetes was evidenced by the results of a standard intravenous glucose-tolerance test, insulin response to the glucose load, and urinary glucose excretion. The results of the urinary clearances for urea, creatinine, and phosphate indicated minimal renal toxicosis and that the toxicosis was localized to the tubules. 相似文献
612.
Hidetoshi Minamiyama Masafumi Shimizu Hitoshi Kunoh Tamotsu Furumai Yasuhiro Igarashi Hiroyasu Onaka Ryuji Yoshida 《Journal of General Plant Pathology》2003,69(1):65-70
An endophytic actinomycete, isolate R-5 of Streptomyces galbus Frommer, that has promising potential as a biocontrol agent was originally isolated from field-grown rhododendron. In this
study, the mode of entry of R-5 into leaves of tissue-cultured seedlings of rhododendron was investigated in connection with
its production of cell wall-degrading enzymes. Light and scanning electron microscopy (SEM) revealed that R-5 grew on leaf
surfaces and entered leaf tissues via stomata and that the internal mycelia grew out of stomata after colonization in host
tissues. Micromanipulation at the SEM level demonstrated a prominent depression in the host surface at the interfaces with
the mycelia, suggesting that such a depression could be caused by degradation of cell wall components by hydrolytic enzymes
secreted from R-5 mycelia. In subsequent plate assays, R-5 produced cellulase, pectinase, xylanase, and nonspecific esterase
when cultured in liquid medium. Moreover, R-5 multiplied in mineral medium containing cellulose, pectin, or xylan as a single
carbon source. Thus, R-5 mycelia could degrade host cell walls at contact sites and probably utilize the degradation products
as carbon sources.
Received: May 16, 2002 / Accepted: July 9, 2002 相似文献
613.
Taiko BANDA Dhanapati NEUPANEY Jin-bo KIM Makoto ISHIOROSHI Katsuji MARUKAWA Hitoshi CHIBA Tomoo ITO Kunihiko SAMEJIMA 《Animal Science Journal》2004,75(2):155-159
The present immunohistological study was conducted to investigate the localization of fatty acid translocase CD36 (FAT/CD36) in the skeletal muscle (Biceps femoris) fibers of bovine, swine and ovine. The results showed that CD36 was mainly localized in type I muscle fiber of these animals. In contrast, FAT/CD36 localization in type II fiber was insignificant in the types of muscle in the present experiments, suggesting that type II fiber of bovine, swine and ovine might lack fatty acid translocase FAT/CD36. 相似文献
614.
Makoto OTSUKA Aiko ISHIDA Yumi NAKAYAMA Mamoru SAITO Makoto YAMAZAKI Hitoshi MURAKAMI Yutaka NAKAMURA Mitsuto MATSUMOTO Katsuhiro MAMOTO Ryozo TAKADA 《Animal Science Journal》2004,75(3):225-229
Nondigestible oligosaccharides are not digested in the small intestine, but are fermented by bacteria colonizing in the large intestine. Physiological effects of non‐digestible oligosaccharides have been considered to be conferred by the fermentation of bacteria colonizing in the large intestine. Because cellooligosaccharide is a non‐digestible oligosaccharide, various physiological effects are expected. However, physiological functions of cellooligosaccharide are not well understood. This experiment was conducted to clarify the effect of dietary supplementation with cellooligosaccharide on the growth performance in weanling pigs. The result showed that average daily gain was significantly higher (P < 0.05) in pigs fed a diet supplemented with cellooligosaccharide than in pigs without cellooligosaccharide. There was a tendency to increasing average daily feed intake in pigs with cellooligosaccharide, though the significant difference was not detected (P = 0.18). Feed efficiency and nutrient digestibility of feces and ileum were not changed by feeding cellooligosaccharide. In addition, blood urea nitrogen was significantly higher (P < 0.05) in pigs fed the diet supplemented with cellooligosaccharide than in pigs without cellooligosaccharide. The concentrations of acetic and iso‐valeric acids in the cecum of pigs fed the diet with cellooligosaccharide tended to be higher (P < 0.10) than those without cellooligosaccharide. The results obtained in this study demonstrated that dietary supplementation with cellooligosaccharide improves growth performance in weanling pigs. 相似文献
615.
Kaneko K Uehara K Hayashidani H Ogawa M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(10):1171-1173
The present study was made to know the morphology of the initial invasion and lesions involved in the intestinal colonization of Yersinia enterocolitica serovar O3 in the epithelium of Peyer's patches of mice. Microfold (M) cells formed a specific structure like a pseudopodium and the bacteria were observed on the surface of the pseudopodium-like structure 4 hr after oral administration of serovar O3. The colonies of serovar O3 were observed in the epithelium and the lamina propria of the Peyer's patches dome region, and the bacteria grown in the Peyer's patches were in direct contact with the lumen without covered with the host tissue 24 hr after the administration. 相似文献
616.
Takai H Miyoshi A Yamazaki M Adachi K Katagiri K Arakawa H Katsuyama K Ito T Fujii E Hayashi S Kato A Suzuki M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(9):943-950
Granulocyte colony-stimulating factor (G-CSF) is widely used to mobilize peripheral blood stem cells, and expected to restore cardiac function for patients with coronary artery diseases as a consequence of progression of atherosclerosis. Safety issues related to the administration of G-CSF to these patients, however, are still under study. The animal model for atherosclerosis was produced by feeding miniature swine a high-cholesterol diet for 3 months. G-CSF (5 or 10 microg/kg/day) was given to the animal model by daily subcutaneous injections for 10 days and 20 main arteries were evaluated pathologically. In addition, the general toxicological effects were studied on clinical signs, body weight, hematology, blood chemistry and pathology. In the G-CSF-treated groups, a variety of changes related to the major pharmacological activity of G-CSF including an increase in white blood cell (WBC) counts were observed. In many arteries, atherosclerotic lesions similar to Type I-V of the proposed classification by the American Heart Association were observed. No effects of the G-CSF treatment were seen on the histopathological findings, incidence, severity or distribution of atherosclerotic lesions. In addition, no infiltration of neutrophils to the lesions was observed. These findings suggest that the administration of G-CSF causes neither exacerbation or modification of atherosclerotic lesions nor adverse changes despite that a sufficient increase in WBC counts could be achieved in the peripheral blood. 相似文献
617.
618.
Saori Tatematsu Seiji Usui Takahiro Kanai Yuichi Tanaka Wataru Hyakunari Seiya Kaneko Kouki Kanou Mitsuhiko Sano 《Fisheries Science》2014,80(3):555-568
To determine the effects of artificial headlands (groins with an anchor-shaped head portion) on the surf-zone fish assemblage structure of a sandy beach with strong wave action, three study sites (headland site sheltered behind the head portion, pocket-beach site between two adjacent headlands and exposed reference site without headlands) were sampled on the Kashimanada Coast, central Japan, in summer and autumn in 2012 and 2013. Distinct differences in physical conditions were recognized between the headland site and the other two sites throughout the study period, the former being characterized by lower wave height and turbidity, finer sediment particles and richer detritus. The fish assemblage structures also differed among the sites, the headland site supporting greater species and individual numbers. Cluster analysis showed clear differences in species composition of fish assemblages between the headland site and the other two sites. In addition, small-sized fishes tended to be more abundant at the headland site compared with the other two sites, although no differences were apparent in fish assemblage structure between the pocket-beach and reference sites. These results suggested that the presence of headlands had marked effects on the surf-zone fish assemblage structure in the study area. 相似文献
619.
620.
Abnormal morphology in megalopae of the swimming crab,Portunus trituberculatus during seed production: causes and prevention
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Shigeki Dan Yuming Sui Takaaki Kaneko Satoshi Takeshima Daisuke Ojima Tatsuo Miyoshi Kaoru Hamano Masakazu Ashidate Katsuyuki Hamasaki 《Aquaculture Research》2016,47(10):3189-3202
The occurrence of morphologically immature megalopae, which retain zoeal features such as dorsal spines and furcae of telson, is closely correlated with larval mass mortality during seed production of the swimming crab Portunus trituberculatus in Japanese hatcheries. To determine the cause of immature megalopal morphology, zoeae were reared with various supplementary schedules and density of diets (rotifer, Artemia and phytoplanktons including Chlorella vulgaris and Nannochloropsis oculata). In addition, to assess the relationship between immature morphology and endocrine control, the effect of causative dietary factor was compared with the effect of eyestalk ablation at various timing during zoeal development. Megalopal immature morphology was more distinct in Chlorella‐supplemented groups than in Nannochloropsis‐supplemented groups. High density Chlorella supplementation was associated with the highest incidence of immaturity and resulted in larval mass mortality. The premoult of the third zoeal stage was identified as a critical period at which Chlorella supplementation led to the highest incidence of immaturity. This critical period coincided with the critical period at which larval metamorphosis was regulated by the eyestalk neurosecretory system. Our results suggested that the occurrence of immature megalopal morphology under culture conditions is most likely caused by phytoplankton (especially, Chlorella) supplementation, which disrupts the endocrine regulation. On the basis of our results, we successfully prevented the occurrence of immature megalopal morphology in 500 L tanks by excluding the influence of phytoplankton before the critical period (i.e. discontinuing phytoplankton supplementation and supplying rotifer cultured with non‐phytoplankton materials). 相似文献