全文获取类型
收费全文 | 140篇 |
免费 | 10篇 |
专业分类
林业 | 9篇 |
农学 | 7篇 |
16篇 | |
综合类 | 26篇 |
农作物 | 7篇 |
水产渔业 | 12篇 |
畜牧兽医 | 63篇 |
园艺 | 3篇 |
植物保护 | 7篇 |
出版年
2023年 | 3篇 |
2021年 | 3篇 |
2020年 | 9篇 |
2019年 | 1篇 |
2018年 | 5篇 |
2017年 | 4篇 |
2016年 | 5篇 |
2015年 | 5篇 |
2014年 | 4篇 |
2013年 | 9篇 |
2012年 | 14篇 |
2011年 | 12篇 |
2010年 | 6篇 |
2009年 | 7篇 |
2008年 | 5篇 |
2007年 | 5篇 |
2006年 | 6篇 |
2005年 | 2篇 |
2004年 | 6篇 |
2003年 | 4篇 |
2002年 | 5篇 |
2001年 | 4篇 |
2000年 | 4篇 |
1999年 | 1篇 |
1996年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1980年 | 1篇 |
1975年 | 1篇 |
1972年 | 2篇 |
1967年 | 2篇 |
1966年 | 1篇 |
1947年 | 1篇 |
1900年 | 1篇 |
1880年 | 1篇 |
排序方式: 共有150条查询结果,搜索用时 15 毫秒
41.
Evidence of rapid transfer and bioaccumulation of Microcystin‐LR poses potential risk to freshwater prawn Macrobrachium rosenbergii (de Man) 下载免费PDF全文
Li‐Ping Liu Xiao‐Ming Su Tao‐Ying Chen Kang Li Jia Zhan Hillary Egna James Diana 《Aquaculture Research》2016,47(10):3088-3097
Microcystins accumulate in aquatic organisms and can be transferred to higher trophic levels, eventually affecting vector animals and consumers. We examined three levels of an aquatic food chain (Microcystis aeruginosa, Daphnia magna and Macrobrachium rosenbergii) to identify the transfer efficiency and risk of microcystin on prawns. Samples were analysed using ultra performance liquid chromatography‐mass spectrometry (MS)/MS and microcystin‐LR (MC‐LR) distributions in prawn tissues were studied. The results showed that prawns accumulate MC‐LR both directly from M. aeruginosa and indirectly through D. magna which was pre‐exposed to M. aeruginosa. MC‐LR was detected in the gills, digestive tracts and hepatopancreas of the prawns 2 h after exposure. MC‐LR accumulated in prawns to 0.49 ± 0.04 μg g?1 dry weight in hepatopancreas within 24 h, while it was not detected in muscle samples, and rarely appeared in blood samples in such a short period. Although MC‐LR was not detected in muscle, the head including hepatopancreas of the prawns accumulated troublesome amounts of MC‐LR. These results demonstrate that microcystis blooms in prawn farming potentially pose a risk to human consumers, although prawns may be exposed to the bloom for a very short time, hence regular monitoring of blue green algae population is recommended. 相似文献
42.
Richard M Hillary Ann L Preece Campbell R Davies Hiroyuki Kurota Osamu Sakai Tomoyuki Itoh Ana M Parma Doug S Butterworth James Ianelli Trevor A Branch 《Fish and Fisheries》2016,17(2):469-482
There is considerable international concern and scientific debate about the current state and future of tuna stocks worldwide and the capacity of Regional Fisheries Management Organisations to manage the associated fisheries effectively. In some cases, this concern has extended to predictions of imminent collapse with minimal chances of recovery, even under a commercial catch moratorium. As a viable alternative to a full fishery closure, the Commission for the Conservation of Southern Bluefin Tuna (CCSBT) has adopted a scientifically tested, adaptive rebuilding strategy for the depleted southern bluefin tuna (Thunnus maccoyii) stock. The management procedure (MP) adopted involves a harvest control rule that fully specifies the total allowable catch as a function of key indicators of stock status, adjusting future harvest levels every three years so as to meet the rebuilding targets agreed by CCSBT. It was chosen from a subset of candidate MPs selected following extensive simulation testing. This involved first selecting a wide range of plausible scenarios for stock status and input data, ranging from pessimistic to optimistic, against which the alternative candidate MPs were tested to ensure that they were robust to important uncertainties. This is the first time that a comprehensively evaluated MP has been adopted for an internationally managed tuna stock. Both the process and the outcomes have broad applicability to other internationally managed stocks. 相似文献
43.
Odugbo MO Ogunjumo SO Chukwukere SC Kumbish PR Musa A Ekundayo SO Okewole PA Nwankpa ND Itodo AE Haruna G 《Veterinary journal (London, England : 1997)》2009,181(3):340-342
Clinical signs of severe bronchopneumonia, including anorexia, coughing, nasal discharge, dyspnoea, diarrhoea, distension of the neck, lethargy, recumbency, lameness preceding collapse, and death were observed among a herd of Holstein–Friesian dairy cattle. The outbreak occurred over a 30-day period, and attack and case-fatality rates were 0.4% and 50%, respectively. At necropsy, extensive consolidation in the cranioventral parts of the lungs was observed. Histologically, a severe acute bronchopneumonia with slight pleuritis was present. Both pathological and bacteriological evaluation of the lungs incriminated Histophilus somni (heavy growth). Supplementary laboratory investigations also isolated Clostridium and Klebsiella species (scanty growth) from the lungs. Histophilosis in cattle was confirmed for the first time in Nigeria. 相似文献
44.
The experimental induction of pneumonic pasteurellosis in groups of conventionally reared lambs by 8 serovars (A1, A2, A6, A7, A8, A9, T10, and A11) and untypable (UT) strains of Mannheimia haemolytica (Mh) were examined and compared. The groups of lambs were inoculated intratracheally with 1.4 x 10(8) +/- 0.6 x 10(8) (mean +/- SD) colony-forming units of the Mh serovars or UT isolates in the 6-hour log phase of growth. The variables measured as indicators of disease severity were clinical score, percentage lung consolidation and microbiological re-isolation. The clinical parameters for each group were computed daily for 6 days post infection and the lambs which died were necropsied while the remaining lambs were killed on day 7 pi and the extent of lung consolidation was measured. Clinically, the mean scores for the M. haemolytica serovars were A1 (6.1), A2 (18.8), A6 (0.5), A7 (17.4) and A9 (8.5). The mean percent lung lesion scores for M. haemolytica serovars were A1 (12.5), A2 (66.3), A6 (5.0), A7 (51.3), A9 (33.8) and A11 (2.5). The percent mean pneumonic lung lesions recorded for groups inoculated with A2, A7 and A9 were significantly (P < 0.05) higher than the extent of lung lesions in the other groups. A statistically significant correlation was observed between clinical scores and the severity of the lung lesions (r = 0.96, P < 0.01). High titres of M. haemolytica were recovered from lung lesions, with 10 to 100 times the number of organisms inoculated being present in the lung lesions of lambs inoculated with serovars A2 and A7. These data indicate that although M. haemolytica serovars A1, A2, A6, A7, A9 and A11 are important primary lung pathogens of lambs, serovars A2, A7, and A9 are to be regarded as highly virulent strains that have a greater predilection than the other serovars for causing pneumonia in lambs. 相似文献
45.
46.
Polar P de Muro MA Kairo MT Moore D Pegram R John SA Roach-Benn C 《Veterinary parasitology》2005,134(1-2):159-167
Experiments were conducted to determine if Metarhizium anisopliae isolates which are capable of growth at cattle surface temperatures could produce pathogenicity to Boophilus microplus in laboratory and field studies. The diurnal temperature fluctuation on the surface of cattle was monitored. The temperature tolerance of M. anisopliae isolates (ARSEF3297 and IMI386697) was determined and their pathogenicity to B. microplus compared at a standard bioassay temperature (28 degrees C) and at a temperature similar to the cattle surface (31-35 degrees C). The effect of the two isolates on the B. microplus population on cattle under field conditions was determined. The temperature of the fore udder, rear udder, ribs and neck regions of the mixed Holstein cattle fluctuated between 30 and 35 degrees C, in a similar pattern to the prevailing environmental temperature. However, wider fluctuations were obtained on the ears (28-35 degrees C) and spine (30-41 degrees C). The colony radius of both isolates declined as temperature increased, however, the growth of IMI386697 was five times greater than ARSEF3297 at 34 degrees C. At 28 degrees C, the pathogenicity of both isolates to B. microplus was similar, however, at 31-35 degrees C, IMI386697 was more pathogenic than ARSEF3297. Both isolates reduced the B. microplus population on cattle in comparison to the control formulation. However, IMI386697 (8.5+/-0.64 ticks/animal) produced a greater reduction in tick numbers than ARSEF3297 (19.1+/-0.64 ticks/animal). M. anisopliae was re-isolated from 8.9% of the ticks collected from IMI386697 treated cattle as compared to 2.8% of ticks from ARSEF3297 treated cattle. 相似文献
47.
A common approach to land use change analyses in multidisciplinary landscape-level studies is to delineate discrete forest
and non-forest or urban and non-urban land use categories to serve as inputs into sets of integrated sub-models describing
socioeconomic and ecological processes. Such discrete land use categories, however, may be inappropriate when the socioeconomic
and ecological processes under study are sensitive to a range of human habitation. In this paper, we characterize the spatial
dynamic distribution of humans throughout the forest landscape of western Oregon (USA). We develop an empirical model describing
the spatial distribution and rate of change in historic building densities as a function of a gravity index of development
pressure, existing building densities, slope, elevation, and existing land use zoning. We use the empirical model to project
changes in building densities that are applied to a 1995 base map of building density to describe future spatial distributions
of buildings over time. The projected building density maps serve as inputs into a multidisciplinary landscape-level analysis
of socioeconomic and ecological processes in Oregon's Coast Range Mountains.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
48.
Katherine M.Halloran Emily C.Hoskins Claire Stenhouse Robyn M.Moses Kathrin A.Dunlap M.Carey Satterfield Heewon Seo Gregory A.Johnson Guoyao Wu Fuller W.Bazer 《畜牧与生物技术杂志(英文版)》2021,(3):952-971
Background: Administration of progesterone(P4) to ewes during the first 9 to 12 days of pregnancy accelerates blastocyst development by day 12 of pregnancy, likely due to P4-induced up-regulation of key genes in uterine epithelia responsible for secretion and transport of components of histotroph into the uterine lumen. This study determined if acceleration of blastocyst development induced by exogenous P4 during the pre-implantation period affects fetal-placental development on day 125 of pregnancy. Suffolk ewes(n = 35) were mated to fertile rams and assigned randomly to receive daily intramuscular injections of either corn oil vehicle(CO, n = 18) or 25 mg progesterone in CO(P4, n = 17) for the first 8 days of pregnancy. All ewes were hysterectomized on day 125 of pregnancy and: 1) fetal and placental weights and measurements were recorded; 2) endometrial and placental tissues were analyzed for the expression of candidate mRNAs involved in nutrient transport and arginine metabolism; and 3) maternal plasma, fetal plasma, allantoic fluid, and amniotic fluid were analyzed for amino acids,agmatine, polyamines, glucose, and fructose.Results: Treatment of ewes with exogenous P4 did not alter fetal or placental growth, but increased amounts of aspartate and arginine in allantoic fluid and amniotic fluid, respectively. Ewes that received exogenous P4 had greater expression of mRNAs for SLC7 A1, SLC7 A2, SLC2 A1, AGMAT, and ODC1 in endometria, as well as SLC1 A4, SLC2 A5, SLC2 A8 and ODC1 in placentomes. In addition, AZIN2 protein was immunolocalized to uterine luminal and glandular epithelia in P4-treated ewes, whereas AZIN2 localized only to uterine luminal epithelia in CO-treated ewes.Conclusions: This study revealed that exogenous P4 administered in early pregnancy influenced expression of selected genes for nutrient transporters and the expression of a protein involved in polyamine synthesis on day 125 of pregnancy, suggesting a ‘programming' effect of P4 on gene expression that affected the composition of nutrients in fetal-placental fluids. 相似文献
49.
Denwood MJ Love S Innocent GT Matthews L McKendrick IJ Hillary N Smith A Reid SW 《Veterinary parasitology》2012,188(1-2):120-126
The faecal egg count (FEC) is the most widely used means of quantifying the nematode burden of horses, and is frequently used in clinical practice to inform treatment and prevention. The statistical process underlying the FEC is complex, comprising a Poisson counting error process for each sample, compounded with an underlying continuous distribution of means between samples. Being able to quantify the sources of variability contributing to this distribution of means is a necessary step towards providing estimates of statistical power for future FEC and FECRT studies, and may help to improve the usefulness of the FEC technique by identifying and minimising unwanted sources of variability. Obtaining such estimates require a hierarchical statistical model coupled with repeated FEC observations from a single animal over a short period of time. Here, we use this approach to provide the first comparative estimate of multiple sources of within-horse FEC variability. The results demonstrate that a substantial proportion of the observed variation in FEC between horses occurs as a result of variation in FEC within an animal, with the major sources being aggregation of eggs within faeces and variation in egg concentration between faecal piles. The McMaster procedure itself is associated with a comparatively small coefficient of variation, and is therefore highly repeatable when a sufficiently large number of eggs are observed to reduce the error associated with the counting process. We conclude that the variation between samples taken from the same animal is substantial, but can be reduced through the use of larger homogenised faecal samples. Estimates are provided for the coefficient of variation (cv) associated with each within animal source of variability in observed FEC, allowing the usefulness of individual FEC to be quantified, and providing a basis for future FEC and FECRT studies. 相似文献
50.
Bhowmick NA Chytil A Plieth D Gorska AE Dumont N Shappell S Washington MK Neilson EG Moses HL 《Science (New York, N.Y.)》2004,303(5659):848-851
Stromal cells can have a significant impact on the carcinogenic process in adjacent epithelia. The role of transforming growth factor-beta (TGF-beta) signaling in such epithelial-mesenchymal interactions was determined by conditional inactivation of the TGF-beta type II receptor gene in mouse fibroblasts (Tgfbr2fspKO). The loss of TGF-beta responsiveness in fibroblasts resulted in intraepithelial neoplasia in prostate and invasive squamous cell carcinoma of the forestomach, both associated with an increased abundance of stromal cells. Activation of paracrine hepatocyte growth factor (HGF) signaling was identified as one possible mechanism for stimulation of epithelial proliferation. Thus, TGF-beta signaling in fibroblasts modulates the growth and oncogenic potential of adjacent epithelia in selected tissues. 相似文献