N2O fluxes from a Haplic Luvisol under intensive production of lettuce and cauliflower as affected by different N‐fertilization strategies

Vegetable‐production systems often show high soil mineral‐N contents and, thus, are potential sources for the release of the climate‐relevant trace gas N₂O from soils. Despite numerous investigations on N₂O fluxes, information on the impact of vegetable‐production systems on N₂O emissions in regions with winter frost is still rare. This present study aimed at measuring the annual N₂O emissions and the total yield of a lettuce–cauliflower rotation at different fertilization rates on a Haplic Luvisol in a region exposed to winter frost (S Germany). We measured N₂O emissions from plots fertilized with 0, 319, 401, and 528 kg N ha^–1 (where the latter three amounts represented a strongly reduced N‐fertilization strategy, a target value system [TVS] in Germany, and the N amount fertilized under good agricultural practices). The N₂O release from the treatments was 2.3, 5.7, 8.8, and 10.6 kg N₂O‐N ha^–1 y^–1, respectively. The corresponding emission factors calculated on the basis of the total N input ranged between 1.3% and 1.6%. Winter emission accounted for 45% of the annual emissions, and a major part occurred after the incorporation of cauliflower residues. The annual N₂O emission was positively correlated with the nitrate content of the top soil (0–25 cm) and with the N surpluses of the N balance. Reducing the amount of N fertilizer applied significantly reduced N₂O fluxes. Since there was no significant effect on yields if fertilization was reduced from 528 kg N ha^–1 according to “good agricultural practice” to 401 kg N ha^–1 determined by the TVS, we recommend this optimized fertilization strategy.     

Stimulation of acidic reduction of nitrite to nitric oxide by soybean phenolics: possible relevance to gastrointestinal host defense

This study aimed to evaluate the potential of soybean-promoted acidic nitrite reduction and to correlate this activity with the content of phenolics and with the bactericidal activity against Escherichia coli O157:H7. Extracts of embrionary axes and cotyledons enriched in phenolics increased ?NO formation at acidic pH at values that were 7.1 and 4.5 times higher, respectively, when compared to the reduction of the nonenriched extracts. Among the various phenolics accumulated in the soybean extracts, five stimulated nitrite reduction in the following decreasing order of potency: epicatechin gallate, chlorogenic acid, caffeic acid, galic acid and p-coumaric acid. Extracts of embrionary axes presented higher contents of epicatechin gallate and caffeic acid, compared to that of cotyledons, indicating a positive correlation between activity of the extracts and content of phenolics with regard to nitrite reducing activity. Soybean extracts enriched in phenolics interacted synergistically with acidified nitrite to prevent E. coli O157:H7 growth. The results suggest that soybean phenolics may interfere with the metabolism of ?NO in an acidic environment by accelerating the reduction of nitrite, with a potential antimicrobial effect in the stomach.     

In silico structural characteristics and α-amylase inhibitory properties of Ric c 1 and Ric c 3, allergenic 2S albumins from Ricinus communis seeds

The major Ricinus communis allergens are the 2S albumins, Ric c 1 and Ric c 3. These proteins contain a trypsin/α-amylase inhibitor family domain, suggesting that they have a role in insect resistance. In this study, we verified that Ric c 1 and Ric c 3 inhibited the α-amylase activity of Callosobruchus maculatus, Zabrotes subfasciatus, and Tenebrio molitor (TMA) larvae as well as mammalian α-amylase. The toxicity of 2S albumin was determined through its incorporation in C. maculatus larvae as part of an artificial diet. Bioassays revealed that 2S albumin reduced larval growth by 20%. We also analyzed the tridimensional structures of Ric c 1 and Ric c 3 by (a) constructing a comparative model of Ric c 1 based on Ric c 3 NMR structure and (b) constructing the theoretical structure of the Ric c 1-TMA and Ric c 3-TMA complexes. Our biological and theoretical results revealed that Ric c 1 and Ric c 3 are a new class of α-amylase inhibitors. They could potentially be used to help design inhibitors that would be useful in diverse fields, ranging from diabetes treatment to crop protection.     

Growth and regeneration of plantlets in callus cultures of pineapple

Callus cultures were established from the basal region of in vitro-obtained shoot buds on MS medium supplemented with CH + CW + NAA. Such callus cultures, when grown on MS medium devoid of any growth regulators, regenerated shoot buds and optimum regeneration was obtained on MS + CW (5% v/v) medium. Addition of BA did not enhance shoot bud regeneration, but two variants (albino types) were observed among the BA-induced regenerants. The callus-regenerated shoot buds produced multiple shoots when transferred to MS + NAA + IBA + K medium. The plantlets were induced to root on a modified White's medium + NAA + IBA and subsequently transferred to soil.     

Diagnosis and treatment of a chronic atlanto-occipital subluxation in a dog

A 6-year-old Labrador retriever-cross was evaluated for an abnormal gait and head carriage 6 weeks after suffering trauma. The dog was presented with an ambulatory tetraparesis and was reluctant to move his head. Myelography and computed tomography demonstrated a subluxation of the atlanto-occipital joint with compression of the spinomedullary junction and the brain stem by the occipital bone. Removal of the compressive part of the occipital bone resulted in improvement of the clinical signs within 6 weeks, and resolution of clinical signs occurred 8 months after surgery.     

Increased Levels of Aluminium in Forest Soils: Effects on the Stores of Soil Organic Carbon

Acid atmospheric deposition results in increased levels of mobile aluminium (Al) in forest soils. Laboratory studies suggest that increased binding of Al to soil organic matter (SOM) in the forest floor results in decreased mobility of organic matter in soil water, viz. lower concentrations of dissolved organic carbon (DOC). Other laboratory studies indicate decreased decomposition rates of SOM as a result of Al binding. So far, little field evidence supporting these effects of Al on the lability of SOM have been reported. Here we present a field manipulation experiment in mature Norway spruce forest in Norway, where the content of Al in soil and soil water was increased. Increased Al in the forest floor caused a pronounced decrease in the leaching of DOC. Simultaneously, the decomposition rate of SOM decreased by 30% to 40%. This suggests that elevated Al in the forest floor stimulates accumulation of SOM. In a companion paper we present the effect of increased Al on forest vitality.     

Endosperm structure affects the malting quality of barley (Hordeum vulgare L.)

Twenty-seven barley (Hordeum vulgare L.) samples collected from growing sites in Scandinavia in 2001 and 2002 were examined to study the effect of endosperm structure on malting behavior. Samples were micromalted, and several malt characteristics were measured. Samples were classified as having a mealier or steelier endosperm on the basis of light transflectance (LTm). Because endosperm structure is greatly dependent on protein content, three barley sample pairs with similar protein contents were chosen for further analysis. During malting, the steelier barley samples produced less root mass, but showed higher respiration losses and higher activities of starch-hydrolyzing enzymes. Malts made from steelier barley had a less friable structure, with more urea-soluble D hordein and more free amino nitrogen and soluble protein. The reason for these differences may lie in the structure or localization of the hordeins as well as the possible effects of endosperm packing on water uptake and movement of enzymes.     

Two latent variable risk assessment approaches for mixed continuous and discrete outcomes from developmental toxicity data

Measurements of both continuous and discrete outcomes are encountered in many statistical problems. Here we consider the particular context of teratology studies, where quantitative risk asessment is aimed at determining the effect of dose on the probability that an individual fetus is malformed or of low birth weight, both being important measures of teratogenicity. We will introduce two different joint marginal mean models for outcomes of a mixed nature. First, we propose the Plackett-Dale approach, where for each binary outcome it is assumed that there exists an underlying glatent variable. The latent malformation outcomes are then assumed to follow a Plackett distribution. The second approach we consider is a probit approach. Here it is assumed that there exists an underlying continuous variable for each binary outcome, so the joint distribution for weight and malformation can be assumed to follow a multivariate normal distribution. In both cases, specification of the full distribution will be avoided using pseudolikelihood and generalized estimating equations methodology, respectively. Quantitative risk assessment is illustrated using data from two developmental toxicology experiments.